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The map label for this gene is hemK [H]

Identifier: 55980410

GI number: 55980410

Start: 414139

End: 415641

Strand: Reverse

Name: hemK [H]

Synonym: TTHA0441

Alternate gene names: 55980410

Gene position: 415641-414139 (Counterclockwise)

Preceding gene: 55980411

Following gene: 55980407

Centisome position: 22.47

GC content: 74.65

Gene sequence:

>1503_bases
ATGCCGGAGGCCCCGGGGGATACGCCGCTCGCCCTGGACCTGCTCCGCCGCCTGAGGCGGGCCCTCGGCGCCGCCGGGCT
TCCCGAGCTCGAGGCCTGGGACCTCCTCGCCGCGGCCACGGGCTGGCCCCGAAAGGCCCTATATGGCCGCCTCACCTCCT
CCCTGCCCCAAGAGGCCCTTGACCGGGCCGAGGCGCTCCTCAAGCGGCGCCTCCAGGGCTACCCCCTCCAGTACCTGGTG
GGGGAGGTGGAGTTCTTCGGCCTGCCCCTGCGGGTGGAGGAAGGGGTCCTCATCCCCCGCCCCGAGACGGAAGGGCTGGT
GGAGCTCGCCCTAGGCCTCCCCCTGCCCCCCGCCCCCCGGATCCTGGACGTGGGCACGGGCACGGGAGCCATCGCCCTGG
CCCTGAAGCGGGCCCTGCCCGAGGCGGAGGTCTACGCCACGGAGGTGGACCCCAAGGCCCTCGCCCTCGCCCGGGAGAAC
GCCGAGCGGCTGGGCCTTTCCGTGGCCTTCCTCCCCGCGCCCCTCACCGGGGGGCTTAGGGACCTGGACCTGGTGGTCTC
CAACCCCCCCTACCTCCCCGAGGCCTACCGGGAAAAGGCCCCGAGGGAGCTCGGCTACGAAAGCCCCCTGGCCCTCTACG
CCGGGCCGGAAGGCCTCTCGGTGGCCCGCCCCCTGGCGGAGGAGGCCCGCCGATCCCTGAAGCCCGGGGGGTACCTCCTC
CTGGAACTCGCCCCGGAAAACGTCCACCTCCTCGCCCGGGAGCTCCGGGAAAAGGGCTGGAAGGAGGTGGCGGTCCTACC
GGACCTCGCGGGTCGGGACCGGTACCTCCGGGCCCGGAGGCCCCCCGAGGAGGCGGAAGGCGAGGAGGGGCGGCTCCACC
GTCCACACCTCCCGCAAAGCCACCACCCCCACCCGGACCACCCCGGACCGCCCCCGGAGGAGGAGGAGGCCCTGGGCCAG
GTCCGTCTCCGTGGGGAAGCGGGCGTACTCCGGGGGCACGCCCTCCTCCAGAAGGCGCTTCCGGACCCGTTCCCCCACCT
CCAGGAGGACGGCCCGGGCCCGGGGCTCCCCCGTGTTGAGGAAGGTGGCGCTGGCGAGAACCTCCCCCGCGGGCCACAGG
CGCTTTTCCGGGTAAAAGCGGGCCCGGACGAGGAGGACGCCGTCCTCGTACCCTGCCCCTTCCAGAACAGCAAGCCCCGG
GCCCTTGGCCCCGCCGAGAAGCCGCACGCCCTTGAAGCCCAGGAGGAGGGCCCGCCTCCGGGCCGCCTCTTCCAGCGCCA
CGGGGCCCTCCTCCGGGCCCAGGCGCACCTCCCCCAGGTAGAGCCCCTGGCTCAGGCGGGCGAGGCCTTCCGCGAGCTGG
CCCTTCTGCCCCTCCAGCACCCGGGCCTGGACGAGGAGGCCCTGGACCCGCCTCTCCTCCTCGGCGAGCTCCTGGCGTAC
CCGGGCCAGGGCCTCCCGGAGCCGGGAGGCCTCCTCCTCCAGAACCTCCCGGGAGCGCCTTAA

Upstream 100 bases:

>100_bases
GAACCACCCCCGGGTCACCACGGAAAGCCAAGGGGAGGGAGAGGAGCGGCACGTGGTGGTCTACCCCAGGCGCGAGGCCC
AAGGCTGAGGGCCCGAGGCC

Downstream 100 bases:

>100_bases
GTCCAACACCCGTCCCTGGAGGGCCACGAGGTCGGCCTCGAGCCGCTCCCTCTCCCCCAGGAGGGCTTCCCGCTCCTTTC
GGGCCTGCGCCAGCTCCGCC

Product: HemK family methyltransferase

Products: NA

Alternate protein names: M.HindHemKP [H]

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MPEAPGDTPLALDLLRRLRRALGAAGLPELEAWDLLAAATGWPRKALYGRLTSSLPQEALDRAEALLKRRLQGYPLQYLV
GEVEFFGLPLRVEEGVLIPRPETEGLVELALGLPLPPAPRILDVGTGTGAIALALKRALPEAEVYATEVDPKALALAREN
AERLGLSVAFLPAPLTGGLRDLDLVVSNPPYLPEAYREKAPRELGYESPLALYAGPEGLSVARPLAEEARRSLKPGGYLL
LELAPENVHLLARELREKGWKEVAVLPDLAGRDRYLRARRPPEEAEGEEGRLHRPHLPQSHHPHPDHPGPPPEEEEALGQ
VRLRGEAGVLRGHALLQKALPDPFPHLQEDGPGPGLPRVEEGGAGENLPRGPQALFRVKAGPDEEDAVLVPCPFQNSKPR
ALGPAEKPHALEAQEEGPPPGRLFQRHGALLRAQAHLPQVEPLAQAGEAFRELALLPLQHPGLDEEALDPPLLLGELLAY
PGQGLPEPGGLLLQNLPGAP

Sequences:

>Translated_500_residues
MPEAPGDTPLALDLLRRLRRALGAAGLPELEAWDLLAAATGWPRKALYGRLTSSLPQEALDRAEALLKRRLQGYPLQYLV
GEVEFFGLPLRVEEGVLIPRPETEGLVELALGLPLPPAPRILDVGTGTGAIALALKRALPEAEVYATEVDPKALALAREN
AERLGLSVAFLPAPLTGGLRDLDLVVSNPPYLPEAYREKAPRELGYESPLALYAGPEGLSVARPLAEEARRSLKPGGYLL
LELAPENVHLLARELREKGWKEVAVLPDLAGRDRYLRARRPPEEAEGEEGRLHRPHLPQSHHPHPDHPGPPPEEEEALGQ
VRLRGEAGVLRGHALLQKALPDPFPHLQEDGPGPGLPRVEEGGAGENLPRGPQALFRVKAGPDEEDAVLVPCPFQNSKPR
ALGPAEKPHALEAQEEGPPPGRLFQRHGALLRAQAHLPQVEPLAQAGEAFRELALLPLQHPGLDEEALDPPLLLGELLAY
PGQGLPEPGGLLLQNLPGAP
>Mature_499_residues
PEAPGDTPLALDLLRRLRRALGAAGLPELEAWDLLAAATGWPRKALYGRLTSSLPQEALDRAEALLKRRLQGYPLQYLVG
EVEFFGLPLRVEEGVLIPRPETEGLVELALGLPLPPAPRILDVGTGTGAIALALKRALPEAEVYATEVDPKALALARENA
ERLGLSVAFLPAPLTGGLRDLDLVVSNPPYLPEAYREKAPRELGYESPLALYAGPEGLSVARPLAEEARRSLKPGGYLLL
ELAPENVHLLARELREKGWKEVAVLPDLAGRDRYLRARRPPEEAEGEEGRLHRPHLPQSHHPHPDHPGPPPEEEEALGQV
RLRGEAGVLRGHALLQKALPDPFPHLQEDGPGPGLPRVEEGGAGENLPRGPQALFRVKAGPDEEDAVLVPCPFQNSKPRA
LGPAEKPHALEAQEEGPPPGRLFQRHGALLRAQAHLPQVEPLAQAGEAFRELALLPLQHPGLDEEALDPPLLLGELLAYP
GQGLPEPGGLLLQNLPGAP

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=250, Percent_Identity=36, Blast_Score=119, Evalue=8e-27,
Organism=Escherichia coli, GI1787463, Length=269, Percent_Identity=35.6877323420074, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI87082085, Length=209, Percent_Identity=34.4497607655502, Blast_Score=107, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24582226, Length=234, Percent_Identity=33.7606837606838, Blast_Score=108, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 53921; Mature: 53790

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
0.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEAPGDTPLALDLLRRLRRALGAAGLPELEAWDLLAAATGWPRKALYGRLTSSLPQEAL
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCHHHHH
DRAEALLKRRLQGYPLQYLVGEVEFFGLPLRVEEGVLIPRPETEGLVELALGLPLPPAPR
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEECCCEECCCCCCCCHHHHHHCCCCCCCCC
ILDVGTGTGAIALALKRALPEAEVYATEVDPKALALARENAERLGLSVAFLPAPLTGGLR
EEECCCCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCC
DLDLVVSNPPYLPEAYREKAPRELGYESPLALYAGPEGLSVARPLAEEARRSLKPGGYLL
CEEEEECCCCCCCHHHHHCCCHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCEEE
LELAPENVHLLARELREKGWKEVAVLPDLAGRDRYLRARRPPEEAEGEEGRLHRPHLPQS
EEECCCHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHCCCCCHHCCCCCCCCCCCCCCCC
HHPHPDHPGPPPEEEEALGQVRLRGEAGVLRGHALLQKALPDPFPHLQEDGPGPGLPRVE
CCCCCCCCCCCCCHHHHHHHEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
EGGAGENLPRGPQALFRVKAGPDEEDAVLVPCPFQNSKPRALGPAEKPHALEAQEEGPPP
CCCCCCCCCCCHHHHEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCH
GRLFQRHGALLRAQAHLPQVEPLAQAGEAFRELALLPLQHPGLDEEALDPPLLLGELLAY
HHHHHHHCHHEEHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCHHHHHHHHHC
PGQGLPEPGGLLLQNLPGAP
CCCCCCCCCCHHHCCCCCCC
>Mature Secondary Structure 
PEAPGDTPLALDLLRRLRRALGAAGLPELEAWDLLAAATGWPRKALYGRLTSSLPQEAL
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCHHHHH
DRAEALLKRRLQGYPLQYLVGEVEFFGLPLRVEEGVLIPRPETEGLVELALGLPLPPAPR
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEECCCEECCCCCCCCHHHHHHCCCCCCCCC
ILDVGTGTGAIALALKRALPEAEVYATEVDPKALALARENAERLGLSVAFLPAPLTGGLR
EEECCCCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCC
DLDLVVSNPPYLPEAYREKAPRELGYESPLALYAGPEGLSVARPLAEEARRSLKPGGYLL
CEEEEECCCCCCCHHHHHCCCHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCEEE
LELAPENVHLLARELREKGWKEVAVLPDLAGRDRYLRARRPPEEAEGEEGRLHRPHLPQS
EEECCCHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHCCCCCHHCCCCCCCCCCCCCCCC
HHPHPDHPGPPPEEEEALGQVRLRGEAGVLRGHALLQKALPDPFPHLQEDGPGPGLPRVE
CCCCCCCCCCCCCHHHHHHHEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
EGGAGENLPRGPQALFRVKAGPDEEDAVLVPCPFQNSKPRALGPAEKPHALEAQEEGPPP
CCCCCCCCCCCHHHHEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCH
GRLFQRHGALLRAQAHLPQVEPLAQAGEAFRELALLPLQHPGLDEEALDPPLLLGELLAY
HHHHHHHCHHEEHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCHHHHHHHHHC
PGQGLPEPGGLLLQNLPGAP
CCCCCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]