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The map label for this gene is pilT [H]

Identifier: 55980334

GI number: 55980334

Start: 344653

End: 345738

Strand: Direct

Name: pilT [H]

Synonym: TTHA0365

Alternate gene names: 55980334

Gene position: 344653-345738 (Clockwise)

Preceding gene: 55980333

Following gene: 55980335

Centisome position: 18.63

GC content: 65.75

Gene sequence:

>1086_bases
ATGGCCAAAGCCCCAGACGTGGTGGACCTTTTGGCGCTCGCGGTGGAGCGGGGGGCGAGCGACCTGGTGATCACCGTGGG
CCTCCCCCCCATGCTGAAGATCGACGGGGAGTTCCACCCCACGGAGTACGAGCCCCTCACCCCCCAGGAGACCCGCCGCC
TCATGTACGCCCTCATGGACGAGAAGCAGCAGCGGATCTTTGAGGAGGAGAAGGAGCTGGACTTCTCCTTCAGCCTCCCC
GGGAAGGGGCGGTACCGGGTCAACGTCTTCCTGCAAAGGGGAAGCGTGGGGGGGGTCCTAAGGGTCGTGCCGAGCGCCGT
GAAGAGCTTTGAGGAGCTCGGCCTCCCCAAGAACATCGCCGAGATCGCCATGAGCCCAAGGGGCCTCGTCCTGGTCACGG
GGCCCACAGGGTCCGGCAAGAGCACCACCCTGGCCTCCATGATTGACTACATCAACGAACGCAAGGCCTGCCACATCGTC
ACCATTGAGGACCCCATAGAGTTCTTCCACAAGCACAAGCGGGCCATCGTGAACCAACGGGAGATCGGCTCCGACACCAA
GAGCTTCCACAAGGCGCTCCGCAGCGTCCTCCGCCAGGCCCCGGACGTGATCCTCGTGGGGGAGATGCGGGACTACGAGA
CCATCGCCGCCGCCATCACCGCCGCCGAGACGGGGCACCTGGTCATGGGCACCCTCCACACCAACTCCGCCCCGGAGACC
ATAGACCGCATCGTGGACGTCTTCCCGGAAAACCAGCAGGAACAGGTCCGGGTGCAGCTCTCCAACAACCTGGTGGCCGT
CCTCACGCAACAGCTCCTGCCCAAGGCCTTTGGCGGCGGGCGGGTCCTGGCCTACGAGCTCATGATCGCTACCCCAGCCG
TGAGGGCCCTCATCCGGGAGGGGAAGACCCACCAGTTAAGGAGCGTGATCCAGACGGGCGGCCAGTACGGGATGATCACC
ATGGACGCCTGCCTCGCCGACCTCTACCGGCGCAAGCTCATCACCTACGAGATGGGGCTCGCCAGGGCCGTGGACCCCAA
GGAGTTCATGAGGCTCGCGGGCGCGCCCGAGGGCGCCAGGCGGTAA

Upstream 100 bases:

>100_bases
GATGAAGACCCTGAGGGAGGACGGCCTCTACAAGGCCCTCCAAGGGATCACCACCCTCGAGGAGGTCCTGGCGCGTACCA
TTGAGTAAAGGAGGAAAGCG

Downstream 100 bases:

>100_bases
ACTTTCCCCGATGTACGAAGCCGTCATCGGCCTCGAGGTCCACCTGCACCTCAAGACCCGCACCAAGATGTTCTGCGGCT
GCCGGGCGGACTACTTCGGG

Product: type IV pilus assembly protein, pilus retraction protein PilT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MAKAPDVVDLLALAVERGASDLVITVGLPPMLKIDGEFHPTEYEPLTPQETRRLMYALMDEKQQRIFEEEKELDFSFSLP
GKGRYRVNVFLQRGSVGGVLRVVPSAVKSFEELGLPKNIAEIAMSPRGLVLVTGPTGSGKSTTLASMIDYINERKACHIV
TIEDPIEFFHKHKRAIVNQREIGSDTKSFHKALRSVLRQAPDVILVGEMRDYETIAAAITAAETGHLVMGTLHTNSAPET
IDRIVDVFPENQQEQVRVQLSNNLVAVLTQQLLPKAFGGGRVLAYELMIATPAVRALIREGKTHQLRSVIQTGGQYGMIT
MDACLADLYRRKLITYEMGLARAVDPKEFMRLAGAPEGARR

Sequences:

>Translated_361_residues
MAKAPDVVDLLALAVERGASDLVITVGLPPMLKIDGEFHPTEYEPLTPQETRRLMYALMDEKQQRIFEEEKELDFSFSLP
GKGRYRVNVFLQRGSVGGVLRVVPSAVKSFEELGLPKNIAEIAMSPRGLVLVTGPTGSGKSTTLASMIDYINERKACHIV
TIEDPIEFFHKHKRAIVNQREIGSDTKSFHKALRSVLRQAPDVILVGEMRDYETIAAAITAAETGHLVMGTLHTNSAPET
IDRIVDVFPENQQEQVRVQLSNNLVAVLTQQLLPKAFGGGRVLAYELMIATPAVRALIREGKTHQLRSVIQTGGQYGMIT
MDACLADLYRRKLITYEMGLARAVDPKEFMRLAGAPEGARR
>Mature_360_residues
AKAPDVVDLLALAVERGASDLVITVGLPPMLKIDGEFHPTEYEPLTPQETRRLMYALMDEKQQRIFEEEKELDFSFSLPG
KGRYRVNVFLQRGSVGGVLRVVPSAVKSFEELGLPKNIAEIAMSPRGLVLVTGPTGSGKSTTLASMIDYINERKACHIVT
IEDPIEFFHKHKRAIVNQREIGSDTKSFHKALRSVLRQAPDVILVGEMRDYETIAAAITAAETGHLVMGTLHTNSAPETI
DRIVDVFPENQQEQVRVQLSNNLVAVLTQQLLPKAFGGGRVLAYELMIATPAVRALIREGKTHQLRSVIQTGGQYGMITM
DACLADLYRRKLITYEMGLARAVDPKEFMRLAGAPEGARR

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=313, Percent_Identity=46.3258785942492, Blast_Score=275, Evalue=4e-75,
Organism=Escherichia coli, GI1786296, Length=378, Percent_Identity=32.010582010582, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI1789723, Length=242, Percent_Identity=32.2314049586777, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 39949; Mature: 39818

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAPDVVDLLALAVERGASDLVITVGLPPMLKIDGEFHPTEYEPLTPQETRRLMYALMD
CCCCCHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHH
EKQQRIFEEEKELDFSFSLPGKGRYRVNVFLQRGSVGGVLRVVPSAVKSFEELGLPKNIA
HHHHHHHHHHHCCCEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHH
EIAMSPRGLVLVTGPTGSGKSTTLASMIDYINERKACHIVTIEDPIEFFHKHKRAIVNQR
HHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHH
EIGSDTKSFHKALRSVLRQAPDVILVGEMRDYETIAAAITAAETGHLVMGTLHTNSAPET
HCCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHH
IDRIVDVFPENQQEQVRVQLSNNLVAVLTQQLLPKAFGGGRVLAYELMIATPAVRALIRE
HHHHHHHCCCCCHHHEEEHHCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHC
GKTHQLRSVIQTGGQYGMITMDACLADLYRRKLITYEMGLARAVDPKEFMRLAGAPEGAR
CCHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHCHHHCCCHHHHHHHHCCCCCCC
R
C
>Mature Secondary Structure 
AKAPDVVDLLALAVERGASDLVITVGLPPMLKIDGEFHPTEYEPLTPQETRRLMYALMD
CCCCHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHH
EKQQRIFEEEKELDFSFSLPGKGRYRVNVFLQRGSVGGVLRVVPSAVKSFEELGLPKNIA
HHHHHHHHHHHCCCEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHH
EIAMSPRGLVLVTGPTGSGKSTTLASMIDYINERKACHIVTIEDPIEFFHKHKRAIVNQR
HHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHH
EIGSDTKSFHKALRSVLRQAPDVILVGEMRDYETIAAAITAAETGHLVMGTLHTNSAPET
HCCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHH
IDRIVDVFPENQQEQVRVQLSNNLVAVLTQQLLPKAFGGGRVLAYELMIATPAVRALIRE
HHHHHHHCCCCCHHHEEEHHCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHC
GKTHQLRSVIQTGGQYGMITMDACLADLYRRKLITYEMGLARAVDPKEFMRLAGAPEGAR
CCHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHCHHHCCCHHHHHHHHCCCCCCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]