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The map label for this gene is folC [H]

Identifier: 55980312

GI number: 55980312

Start: 324874

End: 326118

Strand: Reverse

Name: folC [H]

Synonym: TTHA0343

Alternate gene names: 55980312

Gene position: 326118-324874 (Counterclockwise)

Preceding gene: 55980313

Following gene: 55980308

Centisome position: 17.63

GC content: 73.33

Gene sequence:

>1245_bases
ATGGACCCCCTCTCCTGGCTCTACGCCCGGCAGGGCCGCTTTGCCCCGGGCCTGGAGCGGATCCGGGCCTTCCTCGCCCG
CCTTGGCCACCCCGAGGAGGCCTATCCCGTGGCCCTCGTGGGGGGGACGAACGGGAAGGGCACCACCGCCCGGGCCCTCG
CCGCCATCCTGGAGGAGGCGGGGTTTAGGGTGGGCCTCTACACCAGCCCCCACCTGGTGGACTTCCGGGAGCGCATCCAG
GTCCAGGGAAGGCCGATCCCCGAGGAGAAGCTCCTCGCGCTTTTGGCGGAGCTCCGTCCCCACGCCGAGCGCCTGGAGGC
GAGCTTCTTTGAGGTTGCCACCGCCCTCGCCCTCCTCCACTTCGCCCGGGAGGGGGTGGAGTTCGCCGTCCTCGAGGTGG
GCCTGGGGGGGCGCCTGGACGCCACCAACGCCACAGAACCCGTCCTCTCCGTGGTCACCAACGTCGGCCACGACCACCTC
GAGGTCCTGGGCCCCACCCTGAAGGACGTGGCCCGGGAGAAGGCGGGGATCTTCCGCGGGGGCGTCCCCGCCCTCACCGC
CGCCCGGGGGGAGGGGCTTGGGGTCTTGCGGGCCGAGGCCTTGGCCCGCAAGACCCCCTTGTGGGTCCTGGGGGAGGACT
TCCACGCCGAGGGGGTGGAGGCCCTGCCCCAGGGCCTCGCCTTCACCCTGCGCCTGGAGAGGACGGGGGAGGCCCTGAGG
CTCACCGCCCGCCTCCTCGGCCCCCATCAGGCGGAGAACCTGGCCCTGGCCGCCCTTTCGGGCCGCCTCCTCGGGGCGGG
CTGGGAGGCGGTGAAGCGGGGCGTGGCCCGGGCGGAAAACCCGGGGAGGCTCCAGCGGCTTCTTTGGCGGGGGAGGGAGC
TCCTTCTGGACGGGGCCCACAACCCGGAGGGGGCCTTGGCCCTGAGGGAGGCCCTACGCTTCCACGGTCTGCTTCCCGCC
GCCTTCGTCCTGGGCTTCAGCCGGGACAAGGACCACGCCGCCATGGCCGAGGCCCTGAGGGGCTTGGGCCCCGTGGTCCT
CACCCGCTACGCCTCCCCGAGAAGCGAGGACCCCAGGGCCCTCCTTCCCCTCTTCCCGGGGGCCAAGGTGGTGGAGGACC
CCCTCGAGGCCCTGGAGGAGGCCTTCTCCCGGGAGGGCCGGGTGGTGGTGGCGGGAAGCCTCTACCTGGTGGGGGAGGTT
CTCCGCCGCCTTGAGGGGCTTCCCGCCGAGGAGCGTTGGCAGTAG

Upstream 100 bases:

>100_bases
CGCCCTCGCCCGGGAGGCCCACCCCGGGGTGGAGGTGCGGGAGGCCGAGGTGACCTTCAAGGGGCTCTGCCCCGCCTGCA
AGGCCGCCCTCAAGGGCTAG

Downstream 100 bases:

>100_bases
GCTGGGCGCTTAGTCCAGGGCCTCGCGGATGCGCTTGGCCGCCTCGAGGAGCTCTTCCAGGGGCCGCACCAGGGCGATGC
GGACGAACCCTTTTCCTCCA

Product: folyl-polyglutamate synthetase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MDPLSWLYARQGRFAPGLERIRAFLARLGHPEEAYPVALVGGTNGKGTTARALAAILEEAGFRVGLYTSPHLVDFRERIQ
VQGRPIPEEKLLALLAELRPHAERLEASFFEVATALALLHFAREGVEFAVLEVGLGGRLDATNATEPVLSVVTNVGHDHL
EVLGPTLKDVAREKAGIFRGGVPALTAARGEGLGVLRAEALARKTPLWVLGEDFHAEGVEALPQGLAFTLRLERTGEALR
LTARLLGPHQAENLALAALSGRLLGAGWEAVKRGVARAENPGRLQRLLWRGRELLLDGAHNPEGALALREALRFHGLLPA
AFVLGFSRDKDHAAMAEALRGLGPVVLTRYASPRSEDPRALLPLFPGAKVVEDPLEALEEAFSREGRVVVAGSLYLVGEV
LRRLEGLPAEERWQ

Sequences:

>Translated_414_residues
MDPLSWLYARQGRFAPGLERIRAFLARLGHPEEAYPVALVGGTNGKGTTARALAAILEEAGFRVGLYTSPHLVDFRERIQ
VQGRPIPEEKLLALLAELRPHAERLEASFFEVATALALLHFAREGVEFAVLEVGLGGRLDATNATEPVLSVVTNVGHDHL
EVLGPTLKDVAREKAGIFRGGVPALTAARGEGLGVLRAEALARKTPLWVLGEDFHAEGVEALPQGLAFTLRLERTGEALR
LTARLLGPHQAENLALAALSGRLLGAGWEAVKRGVARAENPGRLQRLLWRGRELLLDGAHNPEGALALREALRFHGLLPA
AFVLGFSRDKDHAAMAEALRGLGPVVLTRYASPRSEDPRALLPLFPGAKVVEDPLEALEEAFSREGRVVVAGSLYLVGEV
LRRLEGLPAEERWQ
>Mature_414_residues
MDPLSWLYARQGRFAPGLERIRAFLARLGHPEEAYPVALVGGTNGKGTTARALAAILEEAGFRVGLYTSPHLVDFRERIQ
VQGRPIPEEKLLALLAELRPHAERLEASFFEVATALALLHFAREGVEFAVLEVGLGGRLDATNATEPVLSVVTNVGHDHL
EVLGPTLKDVAREKAGIFRGGVPALTAARGEGLGVLRAEALARKTPLWVLGEDFHAEGVEALPQGLAFTLRLERTGEALR
LTARLLGPHQAENLALAALSGRLLGAGWEAVKRGVARAENPGRLQRLLWRGRELLLDGAHNPEGALALREALRFHGLLPA
AFVLGFSRDKDHAAMAEALRGLGPVVLTRYASPRSEDPRALLPLFPGAKVVEDPLEALEEAFSREGRVVVAGSLYLVGEV
LRRLEGLPAEERWQ

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=340, Percent_Identity=33.2352941176471, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI66932990, Length=340, Percent_Identity=33.2352941176471, Blast_Score=126, Evalue=3e-29,
Organism=Escherichia coli, GI1788654, Length=407, Percent_Identity=33.1695331695332, Blast_Score=167, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI71984923, Length=333, Percent_Identity=30.9309309309309, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17553150, Length=333, Percent_Identity=30.9309309309309, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17553148, Length=333, Percent_Identity=30.9309309309309, Blast_Score=129, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6323760, Length=424, Percent_Identity=29.9528301886792, Blast_Score=169, Evalue=9e-43,
Organism=Saccharomyces cerevisiae, GI6324815, Length=311, Percent_Identity=30.8681672025724, Blast_Score=126, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6322718, Length=430, Percent_Identity=23.953488372093, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24641571, Length=298, Percent_Identity=31.5436241610738, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24581568, Length=290, Percent_Identity=27.5862068965517, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 44800; Mature: 44800

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPLSWLYARQGRFAPGLERIRAFLARLGHPEEAYPVALVGGTNGKGTTARALAAILEEA
CCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHC
GFRVGLYTSPHLVDFRERIQVQGRPIPEEKLLALLAELRPHAERLEASFFEVATALALLH
CCEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
FAREGVEFAVLEVGLGGRLDATNATEPVLSVVTNVGHDHLEVLGPTLKDVAREKAGIFRG
HHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHCCCCC
GVPALTAARGEGLGVLRAEALARKTPLWVLGEDFHAEGVEALPQGLAFTLRLERTGEALR
CCCHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCHHHHCCCCEEEEEEECCCHHHH
LTARLLGPHQAENLALAALSGRLLGAGWEAVKRGVARAENPGRLQRLLWRGRELLLDGAH
HHHHHHCCCCHHHHHHHHHCCCHHCCCHHHHHHHHHCCCCHHHHHHHHHCCCCEEEECCC
NPEGALALREALRFHGLLPAAFVLGFSRDKDHAAMAEALRGLGPVVLTRYASPRSEDPRA
CCHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHCCCCCCCCCCE
LLPLFPGAKVVEDPLEALEEAFSREGRVVVAGSLYLVGEVLRRLEGLPAEERWQ
EECCCCCHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MDPLSWLYARQGRFAPGLERIRAFLARLGHPEEAYPVALVGGTNGKGTTARALAAILEEA
CCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHC
GFRVGLYTSPHLVDFRERIQVQGRPIPEEKLLALLAELRPHAERLEASFFEVATALALLH
CCEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
FAREGVEFAVLEVGLGGRLDATNATEPVLSVVTNVGHDHLEVLGPTLKDVAREKAGIFRG
HHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHCCCCC
GVPALTAARGEGLGVLRAEALARKTPLWVLGEDFHAEGVEALPQGLAFTLRLERTGEALR
CCCHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCHHHHCCCCEEEEEEECCCHHHH
LTARLLGPHQAENLALAALSGRLLGAGWEAVKRGVARAENPGRLQRLLWRGRELLLDGAH
HHHHHHCCCCHHHHHHHHHCCCHHCCCHHHHHHHHHCCCCHHHHHHHHHCCCCEEEECCC
NPEGALALREALRFHGLLPAAFVLGFSRDKDHAAMAEALRGLGPVVLTRYASPRSEDPRA
CCHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHCCCCCCCCCCE
LLPLFPGAKVVEDPLEALEEAFSREGRVVVAGSLYLVGEVLRRLEGLPAEERWQ
EECCCCCHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]