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The map label for this gene is ribH

Identifier: 55980305

GI number: 55980305

Start: 319340

End: 319813

Strand: Direct

Name: ribH

Synonym: TTHA0336

Alternate gene names: 55980305

Gene position: 319340-319813 (Clockwise)

Preceding gene: 55980300

Following gene: 55980309

Centisome position: 17.26

GC content: 70.04

Gene sequence:

>474_bases
ATGAAGCCCAAGACCCTCTCCCCCATCCTCACCGCCAAGGGGGTACGGCTCGCCATCGCCGTGGGCCGCTTCAACGAGCG
GGTGACCAAGCTCCTTTTGGAGGGGGCCCTCGAGGCCTACGCCCGCCTGGGCGGGGACCCCGCCGAGGTCCTGGTGGCCT
GGGTCCCCGGCTCCTTTGAGCTTCCCCTGGTGGCCAAGCGCCTGGCCCAAAGGCCCGACGTGGACGCGGTGGTGGCCCTG
GGGGCGGTGATCCGGGGCGAGACCCCCCACTTTGAGTACGTGGCGGGCCAGGCGGCGAGCGGCCTCATGCAGGCCATGCT
CCAGACGGAGAAGCCCATCGTCTTCGGCGTCCTCACCACCAACACCCCCGAGGAGGCCCAGGAGCGCGCCGGGGGCAAGG
CGGGCAACAAAGGCGCCGAGGCGGTCTTCACCGCCATTGAGATGGTGCGCCTTCTGGAGGCTATTTCCCGGTGA

Upstream 100 bases:

>100_bases
CCCTGGACCAAGGCCCCGAGGCCGTAGCGCAAAAGCGAGCCCAACGCCCCGCCCAGGGCCACCAGGAGGTATCGCTCCAC
CCCCAAAGTATATTGGGGCC

Downstream 100 bases:

>100_bases
AGAGGGCGCGGGCGTAGCGGAGGTGGAGGTAGAGGAAGCAGCCGAGGCAGAAGCCGAAGGCCAGGTTGACGAAGGCGAGG
AGGGCCACGAGGAGGGCGAG

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MKPKTLSPILTAKGVRLAIAVGRFNERVTKLLLEGALEAYARLGGDPAEVLVAWVPGSFELPLVAKRLAQRPDVDAVVAL
GAVIRGETPHFEYVAGQAASGLMQAMLQTEKPIVFGVLTTNTPEEAQERAGGKAGNKGAEAVFTAIEMVRLLEAISR

Sequences:

>Translated_157_residues
MKPKTLSPILTAKGVRLAIAVGRFNERVTKLLLEGALEAYARLGGDPAEVLVAWVPGSFELPLVAKRLAQRPDVDAVVAL
GAVIRGETPHFEYVAGQAASGLMQAMLQTEKPIVFGVLTTNTPEEAQERAGGKAGNKGAEAVFTAIEMVRLLEAISR
>Mature_157_residues
MKPKTLSPILTAKGVRLAIAVGRFNERVTKLLLEGALEAYARLGGDPAEVLVAWVPGSFELPLVAKRLAQRPDVDAVVAL
GAVIRGETPHFEYVAGQAASGLMQAMLQTEKPIVFGVLTTNTPEEAQERAGGKAGNKGAEAVFTAIEMVRLLEAISR

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=141, Percent_Identity=55.3191489361702, Blast_Score=151, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6324429, Length=143, Percent_Identity=34.2657342657343, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_THET2 (P61728)

Other databases:

- EMBL:   AE017221
- RefSeq:   YP_005616.1
- ProteinModelPortal:   P61728
- SMR:   P61728
- STRING:   P61728
- GeneID:   2775973
- GenomeReviews:   AE017221_GR
- eggNOG:   COG0054
- HOGENOM:   HBG311126
- OMA:   KAGNKGW
- PhylomeDB:   P61728
- ProtClustDB:   PRK00061
- BioCyc:   TTHE262724:TT_C1647-MONOMER
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 16648; Mature: 16648

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPKTLSPILTAKGVRLAIAVGRFNERVTKLLLEGALEAYARLGGDPAEVLVAWVPGSFE
CCCCCCCHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEECCCCCC
LPLVAKRLAQRPDVDAVVALGAVIRGETPHFEYVAGQAASGLMQAMLQTEKPIVFGVLTT
CCHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
NTPEEAQERAGGKAGNKGAEAVFTAIEMVRLLEAISR
CCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKPKTLSPILTAKGVRLAIAVGRFNERVTKLLLEGALEAYARLGGDPAEVLVAWVPGSFE
CCCCCCCHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEECCCCCC
LPLVAKRLAQRPDVDAVVALGAVIRGETPHFEYVAGQAASGLMQAMLQTEKPIVFGVLTT
CCHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
NTPEEAQERAGGKAGNKGAEAVFTAIEMVRLLEAISR
CCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA