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The map label for this gene is cwlS [H]

Identifier: 55980235

GI number: 55980235

Start: 253473

End: 254249

Strand: Direct

Name: cwlS [H]

Synonym: TTHA0266

Alternate gene names: 55980235

Gene position: 253473-254249 (Clockwise)

Preceding gene: 55980234

Following gene: 55980239

Centisome position: 13.7

GC content: 70.01

Gene sequence:

>777_bases
ATGCGCCGCGCCTTCCTCCTCGCCTTCCTGGGCCTCGCCCTGGCCCAGGCCACCTACACCGTGGCCCCCGGGGACACGCT
CTACTCCATCGCCCGGCGCTACGGCACCACGGTGGAGGAGCTCATGCGCTTAAACGGGCTGGAAAGCTTCCTCCTCCAGC
CGGGGCAGGTCCTCAAGCTCCCCTCGAGGGAAAGGACCCACGTGGTGGCCCCGGGGGACACCCTCTTTTCCCTGGCGCGC
CGCTACGGCACCACCGTGGAAGCCCTCATGCGCTTAAACGGCCTCTCCTCCCCGGAGATCAAGGTGGGGCAGGTCCTGAG
GCTTCCCGAAGAGGGCGAGGCGCCTCCGCCCCCTCCCCCGGAGCCGGAGGCCTTGGACCCGGAAAGCCCCCTCCTCCGGG
CCGTCCTCCGCTACCTGGGGGTGCCCTACAAGTACGGGGCGAACTCCCCCTTGGCCCTGGACTGCTCCGCCTTCGTGGCC
CAGGTCTACGCCGAGCTGGGCGTGGCCCTCCCCCGGACCACCAAGGAGCAGTACCAGGCCTTCCCCCCTGTAGAGGCCCC
GCGCCCGGGGGACCTGGTCTTCTTCAGCTTCGGCGGGAAGGAGGTAGACCACGTGGGGATCTACCTGGGCCGGGGGGTCT
TCGCCCACGCCTCTAGCTACGGAAGCCGGGTGGTCATAGAGAGCCTCGAGGCCCCCTTCTACCGGAAGGTCTACCGGGGG
GCGAGGCGGGTCATGGCCAGTCCGGAACCTCCCCCCGCGCCTTCCGCAACGCCATGA

Upstream 100 bases:

>100_bases
GTTCCGCCCCCTCCTCGCCGACCTGGCCCGCCGCTACCGCAACCTTTTTCCCCAGGAGCCCCTGCCCGAAGGGGTGCGGA
GCCTGCTCAAGGAGGGGTGA

Downstream 100 bases:

>100_bases
GGAGGGCCTCCCCCGGCCTCCCCTCCCCCCGGAAGGCCCGGGCCTCCTCCTCCAGCCAGACCTCCCCCTTGCGGAAGAGC
ACCCCCCGGGCCTCCGCCAA

Product: cell wall-binding endopeptidase

Products: NA

Alternate protein names: Cell wall lytic enzyme associated with cell separation [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MRRAFLLAFLGLALAQATYTVAPGDTLYSIARRYGTTVEELMRLNGLESFLLQPGQVLKLPSRERTHVVAPGDTLFSLAR
RYGTTVEALMRLNGLSSPEIKVGQVLRLPEEGEAPPPPPPEPEALDPESPLLRAVLRYLGVPYKYGANSPLALDCSAFVA
QVYAELGVALPRTTKEQYQAFPPVEAPRPGDLVFFSFGGKEVDHVGIYLGRGVFAHASSYGSRVVIESLEAPFYRKVYRG
ARRVMASPEPPPAPSATP

Sequences:

>Translated_258_residues
MRRAFLLAFLGLALAQATYTVAPGDTLYSIARRYGTTVEELMRLNGLESFLLQPGQVLKLPSRERTHVVAPGDTLFSLAR
RYGTTVEALMRLNGLSSPEIKVGQVLRLPEEGEAPPPPPPEPEALDPESPLLRAVLRYLGVPYKYGANSPLALDCSAFVA
QVYAELGVALPRTTKEQYQAFPPVEAPRPGDLVFFSFGGKEVDHVGIYLGRGVFAHASSYGSRVVIESLEAPFYRKVYRG
ARRVMASPEPPPAPSATP
>Mature_258_residues
MRRAFLLAFLGLALAQATYTVAPGDTLYSIARRYGTTVEELMRLNGLESFLLQPGQVLKLPSRERTHVVAPGDTLFSLAR
RYGTTVEALMRLNGLSSPEIKVGQVLRLPEEGEAPPPPPPEPEALDPESPLLRAVLRYLGVPYKYGANSPLALDCSAFVA
QVYAELGVALPRTTKEQYQAFPPVEAPRPGDLVFFSFGGKEVDHVGIYLGRGVFAHASSYGSRVVIESLEAPFYRKVYRG
ARRVMASPEPPPAPSATP

Specific function: Probably functions as a cell separation enzyme in addition to lytE and lytF [H]

COG id: COG0791

COG function: function code M; Cell wall-associated hydrolases (invasion-associated proteins)

Gene ontology:

Cell location: Cell surface. Note=Localized at cell separation sites and cell poles during late vegetative phase [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1788501, Length=125, Percent_Identity=38.4, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1787944, Length=121, Percent_Identity=37.1900826446281, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788001, Length=136, Percent_Identity=33.8235294117647, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786421, Length=114, Percent_Identity=36.8421052631579, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064
- InterPro:   IPR018392
- InterPro:   IPR002482 [H]

Pfam domain/function: PF01476 LysM; PF00877 NLPC_P60 [H]

EC number: =3.4.19.11 [H]

Molecular weight: Translated: 28096; Mature: 28096

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRAFLLAFLGLALAQATYTVAPGDTLYSIARRYGTTVEELMRLNGLESFLLQPGQVLKL
CCHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHCCCHHHHHHHCCHHHHHHCCCCEEEC
PSRERTHVVAPGDTLFSLARRYGTTVEALMRLNGLSSPEIKVGQVLRLPEEGEAPPPPPP
CCCCCCEEECCCHHHHHHHHHHCHHHHHHHHHCCCCCCCCCHHHEEECCCCCCCCCCCCC
EPEALDPESPLLRAVLRYLGVPYKYGANSPLALDCSAFVAQVYAELGVALPRTTKEQYQA
CCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEECHHHHHHHHHHHHHCCCCCCCHHHHHC
FPPVEAPRPGDLVFFSFGGKEVDHVGIYLGRGVFAHASSYGSRVVIESLEAPFYRKVYRG
CCCCCCCCCCCEEEEECCCCCCHHHHHHHCCCHHHHHHHCCCEEHHHHHCCHHHHHHHHH
ARRVMASPEPPPAPSATP
HHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MRRAFLLAFLGLALAQATYTVAPGDTLYSIARRYGTTVEELMRLNGLESFLLQPGQVLKL
CCHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHCCCHHHHHHHCCHHHHHHCCCCEEEC
PSRERTHVVAPGDTLFSLARRYGTTVEALMRLNGLSSPEIKVGQVLRLPEEGEAPPPPPP
CCCCCCEEECCCHHHHHHHHHHCHHHHHHHHHCCCCCCCCCHHHEEECCCCCCCCCCCCC
EPEALDPESPLLRAVLRYLGVPYKYGANSPLALDCSAFVAQVYAELGVALPRTTKEQYQA
CCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEECHHHHHHHHHHHHHCCCCCCCHHHHHC
FPPVEAPRPGDLVFFSFGGKEVDHVGIYLGRGVFAHASSYGSRVVIESLEAPFYRKVYRG
CCCCCCCCCCCEEEEECCCCCCHHHHHHHCCCHHHHHHHCCCEEHHHHHCCHHHHHHHHH
ARRVMASPEPPPAPSATP
HHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9734814; 9384377 [H]