Click here to switch to the map view.

The map label for this gene is birA [H]

Identifier: 55980194

GI number: 55980194

Start: 219655

End: 220539

Strand: Direct

Name: birA [H]

Synonym: TTHA0225

Alternate gene names: 55980194

Gene position: 219655-220539 (Clockwise)

Preceding gene: 55980193

Following gene: 55980195

Centisome position: 11.87

GC content: 74.58

Gene sequence:

>885_bases
ATGCCCGGGCTTTTGGACCTCCTCACGGAGTCCTGGCAAAGCGGCGAGGCCCTGGCCGAGCGGCTCGGGGTGAGCCGGGC
CGCCGTCTCCAAGGAGGCCAGGCGGCTCCGGGCGGAAGGCTACCCCGTGGAGGTCTCCCGCCGGGGCTACCGCATCCTCC
CCGGGACCCCCCTCCCCCACCTCTTCCACCCCCGGGGCAGGCTGGGGAGGCCCTACCGCTACCTGGGCCGGGTGGGGAGC
ACCCAGGACGTCCTAAGGGCCTGGGCCGAGGAGGGCGCCCCCGAGGGGGCCCTGGTCCTCGCCGAGGTCCAGGAAAGGGG
CCGGGGCAGGCGGGGAAGGCCCTGGGAAAGCCGCCCCGGGGGAAGCCTCACCTTCTCCCTCCTCCTAAGGCCAGGCCTTC
CCCTCTCCGCCCTGGGGCTTTTGCCCCTCCTCGCGGGCCTTGCCCTGCGGGAGGCGGTGGGGGTGGGGGGGCTCAAGTGG
CCCAACGACCTCCTCGCCCCGGACGGCGGCAAGCTCGCGGGGGTCCTCCTCGAGGCCCGGGCCGAGGGGGAGGAGGTGAG
TTACGCCCTCCTGGGCGTGGGGGTGAACGTGGCCTGGGCGCCGGAGGGGGCGGCCTTCCTCCAGGCGTACGCCCCCCTTT
CCCGCCGGGAGGTGCTGGAGGGGTTCCTCCTGAAGCTGGAGGCCCTCCTGCCCCTCCTGCAGACCCCGGAGGCCCTCCTG
GAGCGCTACCGCCAGGCCTCCCTCACCCTGGGCCGAAAGGTGCGGGTGCACACGGGCAAAGGGGTGGTGGAGGGCCTGGC
CGAGGACGTCCTCCCCGACGGGAGCCTCCTCGTGGGCGGGGTGCGGGTGGGAGCGGGGGAGGTGGAGCTTCTGCCCCTCC
TTTGA

Upstream 100 bases:

>100_bases
CATCCTCGGCGTCCGGGCCGAAGTGAAGAGCCGGGACGGCCTCCCCCGGGCCCACCGCCTCATCCTCGGGGACCTCTCCC
AGGTGGAGCTAGCCTGAGGG

Downstream 100 bases:

>100_bases
CCCACCCCATGCGGGCTTGCGCTGGCATGGGGCCCCCAAGGGTATGAGTGAGGTAGGCTTAGATTTCCCCAAGCAAATGT
GCTAGGATGGGGGCAGGTAT

Product: biotin operon repressor/biotin-[acetyl-CoA-carboxylase] synthetase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MPGLLDLLTESWQSGEALAERLGVSRAAVSKEARRLRAEGYPVEVSRRGYRILPGTPLPHLFHPRGRLGRPYRYLGRVGS
TQDVLRAWAEEGAPEGALVLAEVQERGRGRRGRPWESRPGGSLTFSLLLRPGLPLSALGLLPLLAGLALREAVGVGGLKW
PNDLLAPDGGKLAGVLLEARAEGEEVSYALLGVGVNVAWAPEGAAFLQAYAPLSRREVLEGFLLKLEALLPLLQTPEALL
ERYRQASLTLGRKVRVHTGKGVVEGLAEDVLPDGSLLVGGVRVGAGEVELLPLL

Sequences:

>Translated_294_residues
MPGLLDLLTESWQSGEALAERLGVSRAAVSKEARRLRAEGYPVEVSRRGYRILPGTPLPHLFHPRGRLGRPYRYLGRVGS
TQDVLRAWAEEGAPEGALVLAEVQERGRGRRGRPWESRPGGSLTFSLLLRPGLPLSALGLLPLLAGLALREAVGVGGLKW
PNDLLAPDGGKLAGVLLEARAEGEEVSYALLGVGVNVAWAPEGAAFLQAYAPLSRREVLEGFLLKLEALLPLLQTPEALL
ERYRQASLTLGRKVRVHTGKGVVEGLAEDVLPDGSLLVGGVRVGAGEVELLPLL
>Mature_293_residues
PGLLDLLTESWQSGEALAERLGVSRAAVSKEARRLRAEGYPVEVSRRGYRILPGTPLPHLFHPRGRLGRPYRYLGRVGST
QDVLRAWAEEGAPEGALVLAEVQERGRGRRGRPWESRPGGSLTFSLLLRPGLPLSALGLLPLLAGLALREAVGVGGLKWP
NDLLAPDGGKLAGVLLEARAEGEEVSYALLGVGVNVAWAPEGAAFLQAYAPLSRREVLEGFLLKLEALLPLLQTPEALLE
RYRQASLTLGRKVRVHTGKGVVEGLAEDVLPDGSLLVGGVRVGAGEVELLPLL

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=285, Percent_Identity=31.2280701754386, Blast_Score=85, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 31458; Mature: 31327

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGLLDLLTESWQSGEALAERLGVSRAAVSKEARRLRAEGYPVEVSRRGYRILPGTPLPH
CCHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEECCCCEEECCCCCCCC
LFHPRGRLGRPYRYLGRVGSTQDVLRAWAEEGAPEGALVLAEVQERGRGRRGRPWESRPG
CCCCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCCEEEHHHHHHCCCCCCCCCCCCCC
GSLTFSLLLRPGLPLSALGLLPLLAGLALREAVGVGGLKWPNDLLAPDGGKLAGVLLEAR
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHH
AEGEEVSYALLGVGVNVAWAPEGAAFLQAYAPLSRREVLEGFLLKLEALLPLLQTPEALL
CCCCHHEEEEEEECCEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHH
ERYRQASLTLGRKVRVHTGKGVVEGLAEDVLPDGSLLVGGVRVGAGEVELLPLL
HHHHHHHHHHCCEEEEECCCHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEECC
>Mature Secondary Structure 
PGLLDLLTESWQSGEALAERLGVSRAAVSKEARRLRAEGYPVEVSRRGYRILPGTPLPH
CHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEECCCCEEECCCCCCCC
LFHPRGRLGRPYRYLGRVGSTQDVLRAWAEEGAPEGALVLAEVQERGRGRRGRPWESRPG
CCCCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCCEEEHHHHHHCCCCCCCCCCCCCC
GSLTFSLLLRPGLPLSALGLLPLLAGLALREAVGVGGLKWPNDLLAPDGGKLAGVLLEAR
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHH
AEGEEVSYALLGVGVNVAWAPEGAAFLQAYAPLSRREVLEGFLLKLEALLPLLQTPEALL
CCCCHHEEEEEEECCEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHH
ERYRQASLTLGRKVRVHTGKGVVEGLAEDVLPDGSLLVGGVRVGAGEVELLPLL
HHHHHHHHHHCCEEEEECCCHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]