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The map label for this gene is prfB [H]

Identifier: 55980111

GI number: 55980111

Start: 138665

End: 139801

Strand: Direct

Name: prfB [H]

Synonym: TTHA0142

Alternate gene names: 55980111

Gene position: 138665-139801 (Clockwise)

Preceding gene: 55980109

Following gene: 55980116

Centisome position: 7.5

GC content: 67.28

Gene sequence:

>1137_bases
TTGCGCCTCGCTTCGCAATCTGCTATCCTGGTAAAGGTATGGACCTGGAACGCCTCGCGCAACGCCTGGAAGGCCTCAGG
GGGTATCTTTGACATCCCCCAAAAGGAAACCCGTCTAAAAGAGCTGGAGCGGCGCCTCGAGGACCCCTCCCTCTGGAACG
ATCCCGAGGCCGCCCGCAAGGTGAGCCAGGAGGCCGCCCGCCTCCGGCGCACCGTGGACACCTTCCGCTCCCTGGAAAGC
GACCTCCAGGGCCTTTTGGAGCTCATGGAGGAGCTTCCCGCCGAGGAACGGGAGGCCCTCAAGCCCGAGCTGGAGGAGGC
CGCGAAGAAGCTGGACGAGCTCTACCACCAGACCCTCCTCAACTTCCCCCACGCGGAGAAGAACGCCATCCTCACCATCC
AGCCCGGGGCCGGGGGCACGGAGGCCTGCGACTGGGCGGAGATGCTCCTAAGGATGTACACCCGCTTCGCCGAGCGCCAG
GGCTTCCAGGTGGAGGTGGTGGACCTCACCCCTGGGCCCGAGGCGGGCATTGACTACGCCCAGATCCTGGTCAAGGGGGA
GAACGCCTACGGCCTCCTTTCCCCCGAGGCCGGGGTGCACCGCCTGGTGCGCCCTTCCCCCTTTGACGCCTCGGGCCGCC
GCCACACCTCCTTCGCCGGGGTGGAGGTGATCCCCGAGGTGGACGAGGAGGTGGAGGTGGTGCTCAAGCCCGAGGAGCTC
CGCATTGACGTGATGCGGGCCTCGGGGCCCGGGGGCCAGGGGGTGAACACCACGGACTCGGCGGTGCGGGTGGTCCACCT
GCCCACGGGGATCACCGTGACCTGCCAGACCACGCGGAGCCAGATCAAGAACAAGGAACTCGCCCTCAAGATCCTCAAGG
CCCGCCTCTACGAGCTGGAGCGGAAGAAGCGGGAGGAAGAGCTCAAGGCCCTGAGGGGCGAGGTGCGGCCCATAGAGTGG
GGAAGCCAGATCCGGAGCTACGTCCTGGACAAGAACTACGTCAAGGACCACCGCACCGGGCTCATGCGCCACGACCCGGA
AAACGTCCTGGACGGGGACCTCATGGACCTGATCTGGGCGGGCCTGGAGTGGAAGGCGGGCCGCCGCCAGGGGACGGAGG
AGGTGGAGGCGGAGTAG

Upstream 100 bases:

>100_bases
CAGAAGGCCCCTGGGGTACTCCTCCTTCAGCGCTTGCGCCCACCGCTCCACATTCATGTCTTTCACTATAAAACGATGAG
CGTACCTATATCAAGACACC

Downstream 100 bases:

>100_bases
CGCCTCAGTAGACGTCCTCCGGGGCCTTGTACACCTCACCGGTAAGCCAGTAGATGACCCTTTCGGCGATGTTTTCCAGG
TGGTCCCCGAGCCTTTCGTA

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MRLASQSAILVKVWTWNASRNAWKASGGIFDIPQKETRLKELERRLEDPSLWNDPEAARKVSQEAARLRRTVDTFRSLES
DLQGLLELMEELPAEEREALKPELEEAAKKLDELYHQTLLNFPHAEKNAILTIQPGAGGTEACDWAEMLLRMYTRFAERQ
GFQVEVVDLTPGPEAGIDYAQILVKGENAYGLLSPEAGVHRLVRPSPFDASGRRHTSFAGVEVIPEVDEEVEVVLKPEEL
RIDVMRASGPGGQGVNTTDSAVRVVHLPTGITVTCQTTRSQIKNKELALKILKARLYELERKKREEELKALRGEVRPIEW
GSQIRSYVLDKNYVKDHRTGLMRHDPENVLDGDLMDLIWAGLEWKAGRRQGTEEVEAE

Sequences:

>Translated_378_residues
MRLASQSAILVKVWTWNASRNAWKASGGIFDIPQKETRLKELERRLEDPSLWNDPEAARKVSQEAARLRRTVDTFRSLES
DLQGLLELMEELPAEEREALKPELEEAAKKLDELYHQTLLNFPHAEKNAILTIQPGAGGTEACDWAEMLLRMYTRFAERQ
GFQVEVVDLTPGPEAGIDYAQILVKGENAYGLLSPEAGVHRLVRPSPFDASGRRHTSFAGVEVIPEVDEEVEVVLKPEEL
RIDVMRASGPGGQGVNTTDSAVRVVHLPTGITVTCQTTRSQIKNKELALKILKARLYELERKKREEELKALRGEVRPIEW
GSQIRSYVLDKNYVKDHRTGLMRHDPENVLDGDLMDLIWAGLEWKAGRRQGTEEVEAE
>Mature_378_residues
MRLASQSAILVKVWTWNASRNAWKASGGIFDIPQKETRLKELERRLEDPSLWNDPEAARKVSQEAARLRRTVDTFRSLES
DLQGLLELMEELPAEEREALKPELEEAAKKLDELYHQTLLNFPHAEKNAILTIQPGAGGTEACDWAEMLLRMYTRFAERQ
GFQVEVVDLTPGPEAGIDYAQILVKGENAYGLLSPEAGVHRLVRPSPFDASGRRHTSFAGVEVIPEVDEEVEVVLKPEEL
RIDVMRASGPGGQGVNTTDSAVRVVHLPTGITVTCQTTRSQIKNKELALKILKARLYELERKKREEELKALRGEVRPIEW
GSQIRSYVLDKNYVKDHRTGLMRHDPENVLDGDLMDLIWAGLEWKAGRRQGTEEVEAE

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=279, Percent_Identity=38.3512544802867, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI34577120, Length=352, Percent_Identity=33.2386363636364, Blast_Score=161, Evalue=9e-40,
Organism=Homo sapiens, GI166795305, Length=188, Percent_Identity=37.2340425531915, Blast_Score=116, Evalue=3e-26,
Organism=Escherichia coli, GI2367172, Length=345, Percent_Identity=47.536231884058, Blast_Score=304, Evalue=6e-84,
Organism=Escherichia coli, GI1787462, Length=331, Percent_Identity=37.4622356495468, Blast_Score=177, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17542784, Length=245, Percent_Identity=36.3265306122449, Blast_Score=115, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6321295, Length=309, Percent_Identity=36.5695792880259, Blast_Score=162, Evalue=8e-41,
Organism=Drosophila melanogaster, GI19921226, Length=293, Percent_Identity=37.2013651877133, Blast_Score=157, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 42734; Mature: 42734

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLASQSAILVKVWTWNASRNAWKASGGIFDIPQKETRLKELERRLEDPSLWNDPEAARK
CCCCCCCEEEEEEEEECCCCCCEECCCCEECCCCHHHHHHHHHHHCCCCCCCCCHHHHHH
VSQEAARLRRTVDTFRSLESDLQGLLELMEELPAEEREALKPELEEAAKKLDELYHQTLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHHHHHHHH
NFPHAEKNAILTIQPGAGGTEACDWAEMLLRMYTRFAERQGFQVEVVDLTPGPEAGIDYA
CCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHH
QILVKGENAYGLLSPEAGVHRLVRPSPFDASGRRHTSFAGVEVIPEVDEEVEVVLKPEEL
HEEEECCCCCEEECCCHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCEEEEECCHHE
RIDVMRASGPGGQGVNTTDSAVRVVHLPTGITVTCQTTRSQIKNKELALKILKARLYELE
EEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
RKKREEELKALRGEVRPIEWGSQIRSYVLDKNYVKDHRTGLMRHDPENVLDGDLMDLIWA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHHHCCCCCCCCHHHCCHHHHHHHHC
GLEWKAGRRQGTEEVEAE
CCCCCCCCCCCCHHCCCC
>Mature Secondary Structure
MRLASQSAILVKVWTWNASRNAWKASGGIFDIPQKETRLKELERRLEDPSLWNDPEAARK
CCCCCCCEEEEEEEEECCCCCCEECCCCEECCCCHHHHHHHHHHHCCCCCCCCCHHHHHH
VSQEAARLRRTVDTFRSLESDLQGLLELMEELPAEEREALKPELEEAAKKLDELYHQTLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHHHHHHHH
NFPHAEKNAILTIQPGAGGTEACDWAEMLLRMYTRFAERQGFQVEVVDLTPGPEAGIDYA
CCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHH
QILVKGENAYGLLSPEAGVHRLVRPSPFDASGRRHTSFAGVEVIPEVDEEVEVVLKPEEL
HEEEECCCCCEEECCCHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCEEEEECCHHE
RIDVMRASGPGGQGVNTTDSAVRVVHLPTGITVTCQTTRSQIKNKELALKILKARLYELE
EEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
RKKREEELKALRGEVRPIEWGSQIRSYVLDKNYVKDHRTGLMRHDPENVLDGDLMDLIWA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHHHCCCCCCCCHHHCCHHHHHHHHC
GLEWKAGRRQGTEEVEAE
CCCCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]