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The map label for this gene is priA [H]

Identifier: 55980032

GI number: 55980032

Start: 59652

End: 61763

Strand: Reverse

Name: priA [H]

Synonym: TTHA0063

Alternate gene names: 55980032

Gene position: 61763-59652 (Counterclockwise)

Preceding gene: 55980036

Following gene: 55980031

Centisome position: 3.34

GC content: 73.15

Gene sequence:

>2112_bases
GTGGCCCTTCCCCTGCCGCTTCCGCCCATGAGCTACCTCCCTCCCTTGGGGCAGGAGGGAGAGGAGGCCTTGGGGCGGCG
GGTGGCCGTTCCTTTTCGGGGGGAGGTCCAGGTGGGGGTGGTGGTGGGGGAAGGGGGGCGGCCCTCCCTGAACCTCCGCC
ACGCCATCGCCTACCTGGACCCCGCCCCCTACCTGCGCCCGGAGGAGATCCTCTTTTTGGAAGAGGCGGCCCGCTACCTT
TTCGCCCCCCTGGGCCAGGTCCTGGCGGACTTCCTCCCCCCCTTTCCCCCCTTGCGCCACCGGGTCCGCCTCTACCCGGG
GGCGGACCCGAAGGTCCTGCCCCCGGGGCTTGGGGCCCTGGTGGACTGGCGGGAGGCCCGGGGCTTTGACCCCAAGCTTT
TGGACCTCCTGCGGGAGGCGGGGATGCTGGAGGAGGAGCTCGCCTTCCGGGAGGCGCGGGGGGTGCTGGTGCCCCTGAAG
CCCGCCCACCCCGATCCCCAGCTGGACCGCGTCCTTCAGGTCCTGCGGGAGCTGGGCTTTGCCGAAAGCCAGGCGGCCCT
GGCCCGGGCGGCGGGGGTGGGGGTGGGCCGGGTGCGCCGCCTTGTCCAGGAGGGGTACATCGGCACCGCGTCCCCGGAGG
AGGCCGCCCCGCCCCCGGCGGACGGGGTGGACGTGGCGCCCCTCCACCTCCCCGAGAGGCCCGAGCGGGTCAACGGCGGG
AGGTTTCTGGAACGCGTGCGGGTGCTCAAGGGGCTTTTGGGGGAGGGGGACCACCTGGTCCTCTTCCCCGAGGTGAGCCT
CTTGGAGCGGTTTCTCGCCCACTTCCCCGGGGCCACGCCCTACCACGGGGGGCTTTCCGGCCCGGTCCGGGAGCGGTTTT
TCCGGAGGCCGCGCGGCCTGGTCTTCGCCACCTACGGCGGGCTCCTCCTCCCCTTCACCCCCCGCTCTTTGGTGGTGGTG
GAGGAGGGGAGCGAGAGCTACAAGCTTCCCTCGGGGAGCCGGGCCTTCGTCCCCCCGCTTGCGGAGCTTAGGGCCCGCCT
CCTCGGGGTGCCCCTCACCTACCTCTCCCTGGTGCCCGCGGTGGAGGTTTTGGAGCGAAAGGGTTTCGCCCTGCCCGTGC
CCAAGCCCCGCCTTCTCCTCTTGGACCTCCGGCGGGAGCGGGGCTTTCCCGTCACGGGGCGGGCCCTCGCCCTCCTCCGC
CAGGTGGAGGAGCGGGGGCGGCAGGCCGTGGTCCTCTCCGCCCGCAAGGGGTACAGCGCCCTCCTCCTCTGCCAGGACTG
CGGCTTCCGGCCCATGTGCCCCGACTGCGCCTTGCCCCTGCGGTACCACCGGGAGGGGAAGGGGGCGCTCGTCTGCCACC
AGTGCGGCCACCGCGAGGACCCGCCCCTCCTCTGCCCCCGGTGCGGCTCCCCCCTCCTCGCCCCCAAGGGGCCCGGGGTG
GACTGGATCCGGGAGGCCCTGGCGGAGAGGCTTTCCCTTCCCGTCTACCGCTACGCCGGCGACGGGAAGGACGACCTCAC
CCCCCTCCTCGAGGGCCGGCCGGGGGTGGTGGTGGGGACCACGGCCCTCCTCCGGGGGCCTAGGCTTCCCGACCTCGCCC
TCGTCCTCCTCCCTTTGGCGGACGGCTTCCTCCTGGAGTCGGACTTCCGGGCGGCGGAGCGGTACCACCGCCTTCTCTGG
GCCCTCACGGAGCTCAGGCCCGGGCGGAGGCCCCTTCTCGTCCTCCAAACCTTCACCCCCGAGCACCCCGTGCACCGGGC
CCTCGAGGCGGGGGAGGTGGAGGCCTACCTGTGGCAGGAGAAGGCCCTGCGGGAGGCTCTCAACTACCCGCCGCGGGTGC
GCATGGTGAAGCTTGAGGTGCGCCACCGGAAGGAGGAGCGGGCCCGGGAAAAGGCCTTCGCCCTCCTGGAGGCCTTGCGG
GCCGAGGCGGAGGAGGGGGAGGTCCTGGGCCCCGCCCCCGCTCCCGTGCCCCGGGTGAAGGGGCATTACGTCTTCCACCT
CCTCCTTCGGGGAAGCACGGAGCGGCTCGCCCGCCTCCTCGGCCTCCTGGACCGGCGGCAGTTCAGGCTGGACCCCGACC
CCTTCCACTTCGTGGGGCTTTTGGAGGACTGA

Upstream 100 bases:

>100_bases
AGGAGGGGGACCACCGCGTAGAGGGCGCTGTAGAGGGCCACGCCCGAGAAGAGCACGGCGAACCGCACCCCCTTATGCTA
GGCCTGTGCGGGTGCTTCAG

Downstream 100 bases:

>100_bases
GCGCCCCCAAGGGCGTGCGCCCGGGCGGGTATCCTAAAGGGGTGCAGGCCCGGGCCTGGGTGGAGGTGGACCTCGCCGCC
CTCTGGGCGAACTACCGGCT

Product: primosomal protein N

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 703; Mature: 702

Protein sequence:

>703_residues
MALPLPLPPMSYLPPLGQEGEEALGRRVAVPFRGEVQVGVVVGEGGRPSLNLRHAIAYLDPAPYLRPEEILFLEEAARYL
FAPLGQVLADFLPPFPPLRHRVRLYPGADPKVLPPGLGALVDWREARGFDPKLLDLLREAGMLEEELAFREARGVLVPLK
PAHPDPQLDRVLQVLRELGFAESQAALARAAGVGVGRVRRLVQEGYIGTASPEEAAPPPADGVDVAPLHLPERPERVNGG
RFLERVRVLKGLLGEGDHLVLFPEVSLLERFLAHFPGATPYHGGLSGPVRERFFRRPRGLVFATYGGLLLPFTPRSLVVV
EEGSESYKLPSGSRAFVPPLAELRARLLGVPLTYLSLVPAVEVLERKGFALPVPKPRLLLLDLRRERGFPVTGRALALLR
QVEERGRQAVVLSARKGYSALLLCQDCGFRPMCPDCALPLRYHREGKGALVCHQCGHREDPPLLCPRCGSPLLAPKGPGV
DWIREALAERLSLPVYRYAGDGKDDLTPLLEGRPGVVVGTTALLRGPRLPDLALVLLPLADGFLLESDFRAAERYHRLLW
ALTELRPGRRPLLVLQTFTPEHPVHRALEAGEVEAYLWQEKALREALNYPPRVRMVKLEVRHRKEERAREKAFALLEALR
AEAEEGEVLGPAPAPVPRVKGHYVFHLLLRGSTERLARLLGLLDRRQFRLDPDPFHFVGLLED

Sequences:

>Translated_703_residues
MALPLPLPPMSYLPPLGQEGEEALGRRVAVPFRGEVQVGVVVGEGGRPSLNLRHAIAYLDPAPYLRPEEILFLEEAARYL
FAPLGQVLADFLPPFPPLRHRVRLYPGADPKVLPPGLGALVDWREARGFDPKLLDLLREAGMLEEELAFREARGVLVPLK
PAHPDPQLDRVLQVLRELGFAESQAALARAAGVGVGRVRRLVQEGYIGTASPEEAAPPPADGVDVAPLHLPERPERVNGG
RFLERVRVLKGLLGEGDHLVLFPEVSLLERFLAHFPGATPYHGGLSGPVRERFFRRPRGLVFATYGGLLLPFTPRSLVVV
EEGSESYKLPSGSRAFVPPLAELRARLLGVPLTYLSLVPAVEVLERKGFALPVPKPRLLLLDLRRERGFPVTGRALALLR
QVEERGRQAVVLSARKGYSALLLCQDCGFRPMCPDCALPLRYHREGKGALVCHQCGHREDPPLLCPRCGSPLLAPKGPGV
DWIREALAERLSLPVYRYAGDGKDDLTPLLEGRPGVVVGTTALLRGPRLPDLALVLLPLADGFLLESDFRAAERYHRLLW
ALTELRPGRRPLLVLQTFTPEHPVHRALEAGEVEAYLWQEKALREALNYPPRVRMVKLEVRHRKEERAREKAFALLEALR
AEAEEGEVLGPAPAPVPRVKGHYVFHLLLRGSTERLARLLGLLDRRQFRLDPDPFHFVGLLED
>Mature_702_residues
ALPLPLPPMSYLPPLGQEGEEALGRRVAVPFRGEVQVGVVVGEGGRPSLNLRHAIAYLDPAPYLRPEEILFLEEAARYLF
APLGQVLADFLPPFPPLRHRVRLYPGADPKVLPPGLGALVDWREARGFDPKLLDLLREAGMLEEELAFREARGVLVPLKP
AHPDPQLDRVLQVLRELGFAESQAALARAAGVGVGRVRRLVQEGYIGTASPEEAAPPPADGVDVAPLHLPERPERVNGGR
FLERVRVLKGLLGEGDHLVLFPEVSLLERFLAHFPGATPYHGGLSGPVRERFFRRPRGLVFATYGGLLLPFTPRSLVVVE
EGSESYKLPSGSRAFVPPLAELRARLLGVPLTYLSLVPAVEVLERKGFALPVPKPRLLLLDLRRERGFPVTGRALALLRQ
VEERGRQAVVLSARKGYSALLLCQDCGFRPMCPDCALPLRYHREGKGALVCHQCGHREDPPLLCPRCGSPLLAPKGPGVD
WIREALAERLSLPVYRYAGDGKDDLTPLLEGRPGVVVGTTALLRGPRLPDLALVLLPLADGFLLESDFRAAERYHRLLWA
LTELRPGRRPLLVLQTFTPEHPVHRALEAGEVEAYLWQEKALREALNYPPRVRMVKLEVRHRKEERAREKAFALLEALRA
EAEEGEVLGPAPAPVPRVKGHYVFHLLLRGSTERLARLLGLLDRRQFRLDPDPFHFVGLLED

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=765, Percent_Identity=26.797385620915, Blast_Score=169, Evalue=6e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 77941; Mature: 77810

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALPLPLPPMSYLPPLGQEGEEALGRRVAVPFRGEVQVGVVVGEGGRPSLNLRHAIAYLD
CCCCCCCCCHHHCCCCCCCCHHHHCCEEECCCCCCEEEEEEECCCCCCCCCHHHHEEEEC
PAPYLRPEEILFLEEAARYLFAPLGQVLADFLPPFPPLRHRVRLYPGADPKVLPPGLGAL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCHH
VDWREARGFDPKLLDLLREAGMLEEELAFREARGVLVPLKPAHPDPQLDRVLQVLRELGF
HHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHCCC
AESQAALARAAGVGVGRVRRLVQEGYIGTASPEEAAPPPADGVDVAPLHLPERPERVNGG
CHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
RFLERVRVLKGLLGEGDHLVLFPEVSLLERFLAHFPGATPYHGGLSGPVRERFFRRPRGL
HHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCE
VFATYGGLLLPFTPRSLVVVEEGSESYKLPSGSRAFVPPLAELRARLLGVPLTYLSLVPA
EEEECCCEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHH
VEVLERKGFALPVPKPRLLLLDLRRERGFPVTGRALALLRQVEERGRQAVVLSARKGYSA
HHHHHHCCCCCCCCCCCEEEEEEHHHCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCEE
LLLCQDCGFRPMCPDCALPLRYHREGKGALVCHQCGHREDPPLLCPRCGSPLLAPKGPGV
EEEEECCCCCCCCCCCCCCHHEECCCCCEEEEECCCCCCCCCEECCCCCCCCCCCCCCCH
DWIREALAERLSLPVYRYAGDGKDDLTPLLEGRPGVVVGTTALLRGPRLPDLALVLLPLA
HHHHHHHHHHHCCCEEEECCCCCCCCCHHHCCCCCEEEECHHHHCCCCCCHHHHHHHHHC
DGFLLESDFRAAERYHRLLWALTELRPGRRPLLVLQTFTPEHPVHRALEAGEVEAYLWQE
CCCEECHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHCCCHHHHHHHH
KALREALNYPPRVRMVKLEVRHRKEERAREKAFALLEALRAEAEEGEVLGPAPAPVPRVK
HHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCC
GHYVFHLLLRGSTERLARLLGLLDRRQFRLDPDPFHFVGLLED
CCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECC
>Mature Secondary Structure 
ALPLPLPPMSYLPPLGQEGEEALGRRVAVPFRGEVQVGVVVGEGGRPSLNLRHAIAYLD
CCCCCCCCHHHCCCCCCCCHHHHCCEEECCCCCCEEEEEEECCCCCCCCCHHHHEEEEC
PAPYLRPEEILFLEEAARYLFAPLGQVLADFLPPFPPLRHRVRLYPGADPKVLPPGLGAL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCHH
VDWREARGFDPKLLDLLREAGMLEEELAFREARGVLVPLKPAHPDPQLDRVLQVLRELGF
HHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHCCC
AESQAALARAAGVGVGRVRRLVQEGYIGTASPEEAAPPPADGVDVAPLHLPERPERVNGG
CHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
RFLERVRVLKGLLGEGDHLVLFPEVSLLERFLAHFPGATPYHGGLSGPVRERFFRRPRGL
HHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCE
VFATYGGLLLPFTPRSLVVVEEGSESYKLPSGSRAFVPPLAELRARLLGVPLTYLSLVPA
EEEECCCEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHH
VEVLERKGFALPVPKPRLLLLDLRRERGFPVTGRALALLRQVEERGRQAVVLSARKGYSA
HHHHHHCCCCCCCCCCCEEEEEEHHHCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCEE
LLLCQDCGFRPMCPDCALPLRYHREGKGALVCHQCGHREDPPLLCPRCGSPLLAPKGPGV
EEEEECCCCCCCCCCCCCCHHEECCCCCEEEEECCCCCCCCCEECCCCCCCCCCCCCCCH
DWIREALAERLSLPVYRYAGDGKDDLTPLLEGRPGVVVGTTALLRGPRLPDLALVLLPLA
HHHHHHHHHHHCCCEEEECCCCCCCCCHHHCCCCCEEEECHHHHCCCCCCHHHHHHHHHC
DGFLLESDFRAAERYHRLLWALTELRPGRRPLLVLQTFTPEHPVHRALEAGEVEAYLWQE
CCCEECHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHCCCHHHHHHHH
KALREALNYPPRVRMVKLEVRHRKEERAREKAFALLEALRAEAEEGEVLGPAPAPVPRVK
HHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCC
GHYVFHLLLRGSTERLARLLGLLDRRQFRLDPDPFHFVGLLED
CCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]