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The map label for this gene is nrgA [H]

Identifier: 55980025

GI number: 55980025

Start: 54673

End: 56013

Strand: Direct

Name: nrgA [H]

Synonym: TTHA0056

Alternate gene names: 55980025

Gene position: 54673-56013 (Clockwise)

Preceding gene: 55980022

Following gene: 55980026

Centisome position: 2.96

GC content: 70.84

Gene sequence:

>1341_bases
ATGCGGAAAGGAACCCTCAACGGCATCGCGGCCCTAGGTTTATCGGGACTGGCCCTGGCGGAGGGGGTAGACGGGGCGGA
CACGGCCTGGATGCTGGTCTCCACGGCCCTGGTCCTCCTCATGACCCCGGCCCTGGCCTTCTTCTACGGGGGGCTCGTCC
GGAGCAAGAACGCCTTGAACACCATGATGATGAGTTTTGCCGCCCTGGCCTTCGTGGGCGTGGGCTGGGCGCTTCTCGGG
TACACCCTGGCCTTTGGGGAGGGGGGCCCCCTCCTGGGGGGTCTCGGGCACCTCTTCCTCAGGGGGGTGGGCCTCGAGGC
CCAGGGCACCATCCCCCACGTCCTCTTCCTGGCCTTCCAGGGGACCTTCGCCATTATCACCGCCGCCCTGGTCTCGGGCG
CCCTGGTGGAGAGGATGCGCTTTCCCGCCTACCTGGCCTTCCTCACCCTCTGGGGGCTTTTCGTCTACGCTCCCCTGGCC
CACTGGGTCTGGGGCGGGGGGTTTTTGGGGGCCCTGGGCGCCCTGGACTTCGCCGGAGGGACGGTGGTGCACATCAACGC
CGGCGTCGCCGCCCTGGTGGGCGCCCTGGTCCTAGGCGCGCGGAAGGACTACGGGCGGCAGGCCATCCTGCCCCATAACG
TCCCCTTCACCCTCCTGGGGGCAGCCCTCCTCTGGTTCGGCTGGTTCGGCTTCAACGGGGGAAGCGCCCTGGCGGCCAAC
GCCTCGGCGGCCCTGGCCTTCGCGAACACCATGCTGGCCCCCGCGGCCACCCTCCTGGTCTGGACCCTCCTGGACCTCCT
TCGCACCGGCAAGGCCACGGCGGTGGGGGCGGCCACGGCCATCGTGGTGGGCCTGGTGGCCGTGACCCCGGCCGCAGGCT
TCGTCTCCCCTCTCTCCGCCCTCCTCATCGGGGCGCTTGCCGCCTTCCCCAGCTACTACGTCCTCCTCTGGCGGGCGAGG
ACCCGGCTGGACGACAGCCTGGACGTCTTCGCCGGCCACGGGGTCGGGGGGATCACCGGGGCCCTCCTCACCGGCGTCTT
CGCCGAGAAGGCCTGGAACGGGGTGGCGGACGGCCTCCTCTTCGGCAACCCCATGCAGCTCGGGATCCAGGCGGTGGCGG
TCCTGGCCGCCGTCGTGTACTCCGCCTTGGGAACCTTCGCCCTCCTCAAGCTGGTGGGCCTCCTCACCCCCTTGCGGGCA
GGCCCCAAGGAGGAAGGGGTGGGCCTGGACGTGACCCAGCACGGCGAGGAGGCCTACACCTCGGGCGAGGGGGCGATCCT
CGTCCTCTCGGAAAAGGCCCCCGCGGTCCCGAGCCTCAGGCCCCAGGGAGGTGAGGCATGA

Upstream 100 bases:

>100_bases
CAAGTCTTCTTGTAACGTTCGGATAGCTATTGCAATTTGTCGCAGGATCTATTGACATTTTGCATCCCATCCTCTACCCT
TGGCCCTAAGGGGGTGGAGG

Downstream 100 bases:

>100_bases
AGCTCATCGTGGCCATCGTCCGTCCGGAGAAGCTCAACGAGGTGCTGGAGGCCCTTTTCCAGGCGGAGGTCCGCGGGCTC
ACCCTAAGCCGCGTCCAGGG

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Membrane protein NrgA; Protein AmtB [H]

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MRKGTLNGIAALGLSGLALAEGVDGADTAWMLVSTALVLLMTPALAFFYGGLVRSKNALNTMMMSFAALAFVGVGWALLG
YTLAFGEGGPLLGGLGHLFLRGVGLEAQGTIPHVLFLAFQGTFAIITAALVSGALVERMRFPAYLAFLTLWGLFVYAPLA
HWVWGGGFLGALGALDFAGGTVVHINAGVAALVGALVLGARKDYGRQAILPHNVPFTLLGAALLWFGWFGFNGGSALAAN
ASAALAFANTMLAPAATLLVWTLLDLLRTGKATAVGAATAIVVGLVAVTPAAGFVSPLSALLIGALAAFPSYYVLLWRAR
TRLDDSLDVFAGHGVGGITGALLTGVFAEKAWNGVADGLLFGNPMQLGIQAVAVLAAVVYSALGTFALLKLVGLLTPLRA
GPKEEGVGLDVTQHGEEAYTSGEGAILVLSEKAPAVPSLRPQGGEA

Sequences:

>Translated_446_residues
MRKGTLNGIAALGLSGLALAEGVDGADTAWMLVSTALVLLMTPALAFFYGGLVRSKNALNTMMMSFAALAFVGVGWALLG
YTLAFGEGGPLLGGLGHLFLRGVGLEAQGTIPHVLFLAFQGTFAIITAALVSGALVERMRFPAYLAFLTLWGLFVYAPLA
HWVWGGGFLGALGALDFAGGTVVHINAGVAALVGALVLGARKDYGRQAILPHNVPFTLLGAALLWFGWFGFNGGSALAAN
ASAALAFANTMLAPAATLLVWTLLDLLRTGKATAVGAATAIVVGLVAVTPAAGFVSPLSALLIGALAAFPSYYVLLWRAR
TRLDDSLDVFAGHGVGGITGALLTGVFAEKAWNGVADGLLFGNPMQLGIQAVAVLAAVVYSALGTFALLKLVGLLTPLRA
GPKEEGVGLDVTQHGEEAYTSGEGAILVLSEKAPAVPSLRPQGGEA
>Mature_446_residues
MRKGTLNGIAALGLSGLALAEGVDGADTAWMLVSTALVLLMTPALAFFYGGLVRSKNALNTMMMSFAALAFVGVGWALLG
YTLAFGEGGPLLGGLGHLFLRGVGLEAQGTIPHVLFLAFQGTFAIITAALVSGALVERMRFPAYLAFLTLWGLFVYAPLA
HWVWGGGFLGALGALDFAGGTVVHINAGVAALVGALVLGARKDYGRQAILPHNVPFTLLGAALLWFGWFGFNGGSALAAN
ASAALAFANTMLAPAATLLVWTLLDLLRTGKATAVGAATAIVVGLVAVTPAAGFVSPLSALLIGALAAFPSYYVLLWRAR
TRLDDSLDVFAGHGVGGITGALLTGVFAEKAWNGVADGLLFGNPMQLGIQAVAVLAAVVYSALGTFALLKLVGLLTPLRA
GPKEEGVGLDVTQHGEEAYTSGEGAILVLSEKAPAVPSLRPQGGEA

Specific function: Functions as an ammonium and methylammonium transporter in the absence of glutamine. Required for ammonium utilization at low concentrations or at low pH values, when ammonium is the single nitrogen source. Required for binding of NrgB to the membrane [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=403, Percent_Identity=45.409429280397, Blast_Score=328, Evalue=5e-91,
Organism=Caenorhabditis elegans, GI17550182, Length=446, Percent_Identity=31.8385650224215, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17550180, Length=433, Percent_Identity=30.0230946882217, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI133931100, Length=422, Percent_Identity=28.1990521327014, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI32564042, Length=417, Percent_Identity=27.5779376498801, Blast_Score=123, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324187, Length=424, Percent_Identity=37.5, Blast_Score=232, Evalue=8e-62,
Organism=Saccharomyces cerevisiae, GI6325396, Length=421, Percent_Identity=33.729216152019, Blast_Score=211, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6321559, Length=419, Percent_Identity=31.7422434367542, Blast_Score=199, Evalue=7e-52,
Organism=Drosophila melanogaster, GI161078314, Length=407, Percent_Identity=32.4324324324324, Blast_Score=139, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 45498; Mature: 45498

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKGTLNGIAALGLSGLALAEGVDGADTAWMLVSTALVLLMTPALAFFYGGLVRSKNALN
CCCCCCCHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
TMMMSFAALAFVGVGWALLGYTLAFGEGGPLLGGLGHLFLRGVGLEAQGTIPHVLFLAFQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHC
GTFAIITAALVSGALVERMRFPAYLAFLTLWGLFVYAPLAHWVWGGGFLGALGALDFAGG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCC
TVVHINAGVAALVGALVLGARKDYGRQAILPHNVPFTLLGAALLWFGWFGFNGGSALAAN
EEEEECCCHHHHHHHHHHHCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEECCC
ASAALAFANTMLAPAATLLVWTLLDLLRTGKATAVGAATAIVVGLVAVTPAAGFVSPLSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LLIGALAAFPSYYVLLWRARTRLDDSLDVFAGHGVGGITGALLTGVFAEKAWNGVADGLL
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHH
FGNPMQLGIQAVAVLAAVVYSALGTFALLKLVGLLTPLRAGPKEEGVGLDVTQHGEEAYT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCHHHC
SGEGAILVLSEKAPAVPSLRPQGGEA
CCCCEEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRKGTLNGIAALGLSGLALAEGVDGADTAWMLVSTALVLLMTPALAFFYGGLVRSKNALN
CCCCCCCHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
TMMMSFAALAFVGVGWALLGYTLAFGEGGPLLGGLGHLFLRGVGLEAQGTIPHVLFLAFQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHC
GTFAIITAALVSGALVERMRFPAYLAFLTLWGLFVYAPLAHWVWGGGFLGALGALDFAGG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCC
TVVHINAGVAALVGALVLGARKDYGRQAILPHNVPFTLLGAALLWFGWFGFNGGSALAAN
EEEEECCCHHHHHHHHHHHCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEECCC
ASAALAFANTMLAPAATLLVWTLLDLLRTGKATAVGAATAIVVGLVAVTPAAGFVSPLSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LLIGALAAFPSYYVLLWRARTRLDDSLDVFAGHGVGGITGALLTGVFAEKAWNGVADGLL
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHH
FGNPMQLGIQAVAVLAAVVYSALGTFALLKLVGLLTPLRAGPKEEGVGLDVTQHGEEAYT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCHHHC
SGEGAILVLSEKAPAVPSLRPQGGEA
CCCCEEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8282685; 9353933; 9384377 [H]