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The map label for this gene is mltB [H]

Identifier: 54295756

GI number: 54295756

Start: 3250215

End: 3251177

Strand: Reverse

Name: mltB [H]

Synonym: lpl2846

Alternate gene names: 54295756

Gene position: 3251177-3250215 (Counterclockwise)

Preceding gene: 54295759

Following gene: 54295755

Centisome position: 97.18

GC content: 46.11

Gene sequence:

>963_bases
ATGAAAAAGTGGGGGCTCGCTATTGTTGCACTGATGTTATTTAACCAAATGACCTATGCAAGTCAAAGCTGGAATCAATG
GGTTGCCGGGGTGCGTGAGGAAGCCTTACAACAAGGGATTCCACCCGGTTTATTTGATGAAGCCTTTGCCGGCATTCATG
AACCAAGCCGGAAAATAAAAGGTTTTTTACGCTCTCAACCTGAACACAGGTTAACGTTCATGAAATATCGTAATACCAGA
GCAGACAATTACCGAATTGTGATTGGAAGAAAAGAGTACAAGAGAAACAAGGAAATACTGGAAGAGATTGCCCAACAATA
CGGAGTTAATCCTTGCTTTATTGTGTCGTTTTGGGGTATGGAAACCAGTTACGGCTCCTACATGGGAAATTTCCCGGTAA
TCAATGCGCTGGCTACTTTGGCCTATGATTCAAACCGTAAAGACTTTTTCCGTAAGGAATTGTTCCTCGCTTTACACATT
CTGAAGGGCGGGCATGTCAGCCTGGCTGATTTCAAGGGCGAGTGGGCTGGCGCCTCTGGCCAACCTCAATTTTTACCATC
CAGCTGGGTCAAATACGCTGTAGACTACGATGGGGATGGACGAAAAGACATATGGAAGAGCAAGCCCGACGTTTTTGCCT
CCATAGCGAATTACATGAAACAAAACGGCTGGCAAGCAGGTGAGCCTTGGGCTATTCAAGTGAAGCTTCCTGCCAAGTTT
GATATGAAACTGGAAGGTAAAAGCATTGTCAAACCAGTCAGCGAATGGAATGCTTTAGGTGTGCGCACGGAAAGCGGTGA
ACCCCTGCCCTATCAAAATTTGCAGGCCAGTATCGTACAGCCTTACGGTGGCCCGACCTTCCTGGCGTTTCCCAACTACA
AGATGATCCTGCGTTATAATAACTCCATTTATTATGCCGGGGCTATCGGGTATATGGCTGATAAAATTTGCCAGGGTCAA
TGA

Upstream 100 bases:

>100_bases
GTATGTTTTGAGCGGATTGTTTATTGTAACAATTAAAGAGAAAGAAACGCTGGGCGAAATACATATTATTAAACAATAAA
TATAACTGAGGGGAAGAGCT

Downstream 100 bases:

>100_bases
TTTGCTGGAGACATAAATATCTACTGATGAATTCCACTGGGACAGGCGTTCAGTGGAGTTCTCATAGGCCAAGCTTTTTA
AGCTTTATAAATCATAGGTA

Product: hypothetical protein

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MKKWGLAIVALMLFNQMTYASQSWNQWVAGVREEALQQGIPPGLFDEAFAGIHEPSRKIKGFLRSQPEHRLTFMKYRNTR
ADNYRIVIGRKEYKRNKEILEEIAQQYGVNPCFIVSFWGMETSYGSYMGNFPVINALATLAYDSNRKDFFRKELFLALHI
LKGGHVSLADFKGEWAGASGQPQFLPSSWVKYAVDYDGDGRKDIWKSKPDVFASIANYMKQNGWQAGEPWAIQVKLPAKF
DMKLEGKSIVKPVSEWNALGVRTESGEPLPYQNLQASIVQPYGGPTFLAFPNYKMILRYNNSIYYAGAIGYMADKICQGQ

Sequences:

>Translated_320_residues
MKKWGLAIVALMLFNQMTYASQSWNQWVAGVREEALQQGIPPGLFDEAFAGIHEPSRKIKGFLRSQPEHRLTFMKYRNTR
ADNYRIVIGRKEYKRNKEILEEIAQQYGVNPCFIVSFWGMETSYGSYMGNFPVINALATLAYDSNRKDFFRKELFLALHI
LKGGHVSLADFKGEWAGASGQPQFLPSSWVKYAVDYDGDGRKDIWKSKPDVFASIANYMKQNGWQAGEPWAIQVKLPAKF
DMKLEGKSIVKPVSEWNALGVRTESGEPLPYQNLQASIVQPYGGPTFLAFPNYKMILRYNNSIYYAGAIGYMADKICQGQ
>Mature_320_residues
MKKWGLAIVALMLFNQMTYASQSWNQWVAGVREEALQQGIPPGLFDEAFAGIHEPSRKIKGFLRSQPEHRLTFMKYRNTR
ADNYRIVIGRKEYKRNKEILEEIAQQYGVNPCFIVSFWGMETSYGSYMGNFPVINALATLAYDSNRKDFFRKELFLALHI
LKGGHVSLADFKGEWAGASGQPQFLPSSWVKYAVDYDGDGRKDIWKSKPDVFASIANYMKQNGWQAGEPWAIQVKLPAKF
DMKLEGKSIVKPVSEWNALGVRTESGEPLPYQNLQASIVQPYGGPTFLAFPNYKMILRYNNSIYYAGAIGYMADKICQGQ

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=245, Percent_Identity=32.2448979591837, Blast_Score=108, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 36410; Mature: 36410

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWGLAIVALMLFNQMTYASQSWNQWVAGVREEALQQGIPPGLFDEAFAGIHEPSRKIK
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHH
GFLRSQPEHRLTFMKYRNTRADNYRIVIGRKEYKRNKEILEEIAQQYGVNPCFIVSFWGM
HHHHCCCCHHEEEEEECCCCCCCEEEEEECHHHHCCHHHHHHHHHHHCCCEEEEEEECCC
ETSYGSYMGNFPVINALATLAYDSNRKDFFRKELFLALHILKGGHVSLADFKGEWAGASG
CCCCCHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCC
QPQFLPSSWVKYAVDYDGDGRKDIWKSKPDVFASIANYMKQNGWQAGEPWAIQVKLPAKF
CCCCCCCCHHEEEEECCCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCC
DMKLEGKSIVKPVSEWNALGVRTESGEPLPYQNLQASIVQPYGGPTFLAFPNYKMILRYN
CEEECCHHHHHHHHHHCCEEEECCCCCCCCCCCCHHHEECCCCCCEEEECCCEEEEEEEC
NSIYYAGAIGYMADKICQGQ
CEEEEEEHHHHHHHHHHCCC
>Mature Secondary Structure
MKKWGLAIVALMLFNQMTYASQSWNQWVAGVREEALQQGIPPGLFDEAFAGIHEPSRKIK
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHH
GFLRSQPEHRLTFMKYRNTRADNYRIVIGRKEYKRNKEILEEIAQQYGVNPCFIVSFWGM
HHHHCCCCHHEEEEEECCCCCCCEEEEEECHHHHCCHHHHHHHHHHHCCCEEEEEEECCC
ETSYGSYMGNFPVINALATLAYDSNRKDFFRKELFLALHILKGGHVSLADFKGEWAGASG
CCCCCHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCC
QPQFLPSSWVKYAVDYDGDGRKDIWKSKPDVFASIANYMKQNGWQAGEPWAIQVKLPAKF
CCCCCCCCHHEEEEECCCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCC
DMKLEGKSIVKPVSEWNALGVRTESGEPLPYQNLQASIVQPYGGPTFLAFPNYKMILRYN
CEEECCHHHHHHHHHHCCEEEECCCCCCCCCCCCHHHEECCCCCCEEEECCCEEEEEEEC
NSIYYAGAIGYMADKICQGQ
CEEEEEEHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]