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The map label for this gene is ptsP [H]

Identifier: 54295696

GI number: 54295696

Start: 3181125

End: 3183419

Strand: Reverse

Name: ptsP [H]

Synonym: lpl2784

Alternate gene names: 54295696

Gene position: 3183419-3181125 (Counterclockwise)

Preceding gene: 54295697

Following gene: 54295695

Centisome position: 95.15

GC content: 42.92

Gene sequence:

>2295_bases
ATGCTTAAAATACTTAAACGGATAGTACAAGATGTTACCGCTGCCAGTGATTTGAAAGAAGCACTTGGCATTTTGGTGCA
ACGTATTGATACAGCCATTAATGCAGAGGCTGTTTCCGTTTATCTTATTGATAATAAAAATGCTGAATACGTCTTGATAG
CCACGGAAGGTTTGAACAAGCAGGCAGAGTTTAGAGTCAGAATTGCTCTTGATTGTGGCCTTATCGGGTTGATAGGACGA
AGAGAGGAACCTATCAATATAGAAAATGCTCCCTCTCATCCAGACTTTTATCATAATCCATTAATTGGTGAAGATCACCT
GAACGCTTTTTTAGGCGTCCCTATTATTCAACATAGAAAATTATACGGTGTTTTAGTTGTTCAACAGCTTGAACAGCGTC
ATTTTGATGATGCTGAAGAATCATTTCTTATTACCTTGGCGGCCCAATTAGGCGGGATTATTGCTCATGCAGAGGCAACA
GGTGAATTGGCCGAGTTAACACAACCTAAAAAGGGTATAGGAGTCGACAAGGTAGAAGCGGCTTATACTGCACTGACTGG
AATAGGCAGTGTTCCTGGTATAGGAATTGGTACTGCCGTGGTTGTGTACCCGCCTGCGGATATTGATGCTGTCCCTCGTA
ATCCGGTTGAGGAATTGGAAGATGAAATCAATGCGTTTTATGAAGCGTTGGAAACGACAAGAGATGATATCAGGCGTTTA
AGTAAACGCATGAAAGAAACTGTTGCTGAGGAAGAGCATGCTTTATTCGATGTGTATTTAAGAATTTTGGATAGGGATAG
CTTGGGTGCTGAAGTAGAGCATGTGATTCGTGAAGAGAAAATCAGCGCTCAAGCTGCCCTGGCTACAGTGATCAAGAAAC
ACGTGCAGCAATTTGAAAGCATGGGCGATGATTATCTGCGCGAACGAGCCAGCGATTTCCGTGATTTGGGCCGAAGAGTT
TTGGCGGAATTACAACGTACCCAAAGAGAAGAAATTGTTTATCCGAAGAGAACCATTTTAATAGGAGAAGAAATTACTGC
TGCCGCTCTGGCAGAAGTTCCAGAAGGGCAATTGGCTGGTGTCGTTTCAGCTAAAGGCTCCAATAACTCCCACGTTGCTA
TTTTAGCGAGAGCTCTCGGTGTACCTACTGTGATGGGGTTGAGAGGATTAAAAGTAGAATTGGTATCGCGTCGGGCTTTG
ATTGTTGATGGCTATTATGGGCATGTTTATATCTCTCCTTCCAAATCATTATTAGCGGAATTTAAGCAATTAGCTCAAGA
AGAACAGGAATTAAACCAAAGCCTGGTAAGCTTGCGTGATAAACCAGCTGAAACTACTGATAACTACAGAGTCTCTTTGC
AAGTCAATACCGGATTGGCAATGGATGCCGGTTTATCAATGAGTGTTGGCGCTGAAGGAGTTGGTTTATACCGTTCAGAA
GTACCATTTATGAGTCGAGATCGATTCCCTTCAGAAGATGAGCAGACCATTATTTACAGACAGACTCTCAAGGCCTTTGC
TCCACGTTTGGTAACTATGCGTACCCTGGATATTGGTGGAGACAAAATTCTACCTTATTTTCCAGTTGAAGAGGAAAACC
CTTATTTAGGATGGCGTGGAATAAGGATAACTCTTGATCATCCAGATGTCTTCCTGATTCAGGTTCGTGCCATGATGCGT
GCTAGTGAGGAACTGGATAATTTACGTATCATGTTGCCTATGGTCACAACACTTAGCGAAGTAGAAGAAGCCGTTTATTT
GATTGAGCAGGCTTTTGACGAGCTGGTGGAAGAGGGGTGCGAAATAGTAAAACCCAAAATTGGTGTGATGATTGAAGTGC
CTGCCGCTGCTTATCTGGCAAGAGAAATGGCGAAACGAGTAGATTTTATTTCGGTGGGTAGTAACGATCTTACTCAATAT
TTATTGGCTGTGGATAGAAATAATGCCCGAGTTGCCGCACTTTATGATCCCTTGCATCCAGCCATGTTGCGTACATTGCT
AAAAATAGTCGAAGGAGGGCATGCAGCTGGTGTTGAGGTGAGTATTTGTGGCGAAATGGCCAGCGATCCTTTAGCGGTGA
TCTTGTTAATTGCTATGGGTTTTGACACCTTAAGCATGAATTCCGCCAGTTTACCCAGGGTGAAATGGGTTATTCGCAAT
TTTGCTATCGCGAATGCCCGTAAAATTCTGGCAGAAGTTTTGGAATTTGAACATCCTGCTGAAATACGATTTCATTTGCA
AAAGGCTCTAGAAGAGGAAGGATTAGGGGGATTAATCAGAGCAGGAAAGTCATGA

Upstream 100 bases:

>100_bases
GGTTTATATTCAAGCGTTAAAAGAATTAGAACCTTTGTTAAAAAAAGAACGCCGTACCCCTTATGGCTTAAAACGTAAAA
GAGGTAATCAAAGAGCTTAG

Downstream 100 bases:

>100_bases
TATCCGGTGAGTTACTGATTAATGGAATGATTTACATCATTATTGGTGCTTTTGCAGGAATAATGGCGGGTGCTCTGGGC
GTTGGTGGCGGGCTTATTGT

Product: phosphoenolpyruvate-protein phosphotransferase PtsP

Products: NA

Alternate protein names: Enzyme I-Ntr; Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 764; Mature: 764

Protein sequence:

>764_residues
MLKILKRIVQDVTAASDLKEALGILVQRIDTAINAEAVSVYLIDNKNAEYVLIATEGLNKQAEFRVRIALDCGLIGLIGR
REEPINIENAPSHPDFYHNPLIGEDHLNAFLGVPIIQHRKLYGVLVVQQLEQRHFDDAEESFLITLAAQLGGIIAHAEAT
GELAELTQPKKGIGVDKVEAAYTALTGIGSVPGIGIGTAVVVYPPADIDAVPRNPVEELEDEINAFYEALETTRDDIRRL
SKRMKETVAEEEHALFDVYLRILDRDSLGAEVEHVIREEKISAQAALATVIKKHVQQFESMGDDYLRERASDFRDLGRRV
LAELQRTQREEIVYPKRTILIGEEITAAALAEVPEGQLAGVVSAKGSNNSHVAILARALGVPTVMGLRGLKVELVSRRAL
IVDGYYGHVYISPSKSLLAEFKQLAQEEQELNQSLVSLRDKPAETTDNYRVSLQVNTGLAMDAGLSMSVGAEGVGLYRSE
VPFMSRDRFPSEDEQTIIYRQTLKAFAPRLVTMRTLDIGGDKILPYFPVEEENPYLGWRGIRITLDHPDVFLIQVRAMMR
ASEELDNLRIMLPMVTTLSEVEEAVYLIEQAFDELVEEGCEIVKPKIGVMIEVPAAAYLAREMAKRVDFISVGSNDLTQY
LLAVDRNNARVAALYDPLHPAMLRTLLKIVEGGHAAGVEVSICGEMASDPLAVILLIAMGFDTLSMNSASLPRVKWVIRN
FAIANARKILAEVLEFEHPAEIRFHLQKALEEEGLGGLIRAGKS

Sequences:

>Translated_764_residues
MLKILKRIVQDVTAASDLKEALGILVQRIDTAINAEAVSVYLIDNKNAEYVLIATEGLNKQAEFRVRIALDCGLIGLIGR
REEPINIENAPSHPDFYHNPLIGEDHLNAFLGVPIIQHRKLYGVLVVQQLEQRHFDDAEESFLITLAAQLGGIIAHAEAT
GELAELTQPKKGIGVDKVEAAYTALTGIGSVPGIGIGTAVVVYPPADIDAVPRNPVEELEDEINAFYEALETTRDDIRRL
SKRMKETVAEEEHALFDVYLRILDRDSLGAEVEHVIREEKISAQAALATVIKKHVQQFESMGDDYLRERASDFRDLGRRV
LAELQRTQREEIVYPKRTILIGEEITAAALAEVPEGQLAGVVSAKGSNNSHVAILARALGVPTVMGLRGLKVELVSRRAL
IVDGYYGHVYISPSKSLLAEFKQLAQEEQELNQSLVSLRDKPAETTDNYRVSLQVNTGLAMDAGLSMSVGAEGVGLYRSE
VPFMSRDRFPSEDEQTIIYRQTLKAFAPRLVTMRTLDIGGDKILPYFPVEEENPYLGWRGIRITLDHPDVFLIQVRAMMR
ASEELDNLRIMLPMVTTLSEVEEAVYLIEQAFDELVEEGCEIVKPKIGVMIEVPAAAYLAREMAKRVDFISVGSNDLTQY
LLAVDRNNARVAALYDPLHPAMLRTLLKIVEGGHAAGVEVSICGEMASDPLAVILLIAMGFDTLSMNSASLPRVKWVIRN
FAIANARKILAEVLEFEHPAEIRFHLQKALEEEGLGGLIRAGKS
>Mature_764_residues
MLKILKRIVQDVTAASDLKEALGILVQRIDTAINAEAVSVYLIDNKNAEYVLIATEGLNKQAEFRVRIALDCGLIGLIGR
REEPINIENAPSHPDFYHNPLIGEDHLNAFLGVPIIQHRKLYGVLVVQQLEQRHFDDAEESFLITLAAQLGGIIAHAEAT
GELAELTQPKKGIGVDKVEAAYTALTGIGSVPGIGIGTAVVVYPPADIDAVPRNPVEELEDEINAFYEALETTRDDIRRL
SKRMKETVAEEEHALFDVYLRILDRDSLGAEVEHVIREEKISAQAALATVIKKHVQQFESMGDDYLRERASDFRDLGRRV
LAELQRTQREEIVYPKRTILIGEEITAAALAEVPEGQLAGVVSAKGSNNSHVAILARALGVPTVMGLRGLKVELVSRRAL
IVDGYYGHVYISPSKSLLAEFKQLAQEEQELNQSLVSLRDKPAETTDNYRVSLQVNTGLAMDAGLSMSVGAEGVGLYRSE
VPFMSRDRFPSEDEQTIIYRQTLKAFAPRLVTMRTLDIGGDKILPYFPVEEENPYLGWRGIRITLDHPDVFLIQVRAMMR
ASEELDNLRIMLPMVTTLSEVEEAVYLIEQAFDELVEEGCEIVKPKIGVMIEVPAAAYLAREMAKRVDFISVGSNDLTQY
LLAVDRNNARVAALYDPLHPAMLRTLLKIVEGGHAAGVEVSICGEMASDPLAVILLIAMGFDTLSMNSASLPRVKWVIRN
FAIANARKILAEVLEFEHPAEIRFHLQKALEEEGLGGLIRAGKS

Specific function: Component of the phosphoenolpyruvate-dependent nitrogen- metabolic phosphotransferase system (nitrogen-metabolic PTS), that seems to be involved in regulating nitrogen metabolism. Enzyme I- Ntr transfers the phosphoryl group from phosphoenolpyruvate (PEP)

COG id: COG3605

COG function: function code T; Signal transduction protein containing GAF and PtsI domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GAF domain [H]

Homologues:

Organism=Escherichia coli, GI1789193, Length=768, Percent_Identity=44.2708333333333, Blast_Score=614, Evalue=1e-177,
Organism=Escherichia coli, GI1788756, Length=552, Percent_Identity=35.6884057971014, Blast_Score=350, Evalue=1e-97,
Organism=Escherichia coli, GI1788726, Length=554, Percent_Identity=33.3935018050542, Blast_Score=280, Evalue=2e-76,
Organism=Escherichia coli, GI48994992, Length=482, Percent_Identity=31.9502074688797, Blast_Score=251, Evalue=1e-67,
Organism=Escherichia coli, GI1787994, Length=397, Percent_Identity=26.1964735516373, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI226510935, Length=163, Percent_Identity=28.2208588957055, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003018
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF01590 GAF; PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 84472; Mature: 84472

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKILKRIVQDVTAASDLKEALGILVQRIDTAINAEAVSVYLIDNKNAEYVLIATEGLNK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEECCCCC
QAEFRVRIALDCGLIGLIGRREEPINIENAPSHPDFYHNPLIGEDHLNAFLGVPIIQHRK
CCEEEEEEEECCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHH
LYGVLVVQQLEQRHFDDAEESFLITLAAQLGGIIAHAEATGELAELTQPKKGIGVDKVEA
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEECCCCCHHHHHCCCCCCCCCCHHHH
AYTALTGIGSVPGIGIGTAVVVYPPADIDAVPRNPVEELEDEINAFYEALETTRDDIRRL
HHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SKRMKETVAEEEHALFDVYLRILDRDSLGAEVEHVIREEKISAQAALATVIKKHVQQFES
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGDDYLRERASDFRDLGRRVLAELQRTQREEIVYPKRTILIGEEITAAALAEVPEGQLAG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHCCCCCEEE
VVSAKGSNNSHVAILARALGVPTVMGLRGLKVELVSRRALIVDGYYGHVYISPSKSLLAE
EEEECCCCCCCCEEHHHHHCCCHHHCCCCCEEEEECCCEEEEECCEEEEEECCCHHHHHH
FKQLAQEEQELNQSLVSLRDKPAETTDNYRVSLQVNTGLAMDAGLSMSVGAEGVGLYRSE
HHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCEEECCCCCEECCCCCCCCHHHC
VPFMSRDRFPSEDEQTIIYRQTLKAFAPRLVTMRTLDIGGDKILPYFPVEEENPYLGWRG
CCCCCCCCCCCCCCCHHHHHHHHHHHCCHHEEEEEEECCCCCCCCCCCCCCCCCCCCEEE
IRITLDHPDVFLIQVRAMMRASEELDNLRIMLPMVTTLSEVEEAVYLIEQAFDELVEEGC
EEEEECCCCEEHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIVKPKIGVMIEVPAAAYLAREMAKRVDFISVGSNDLTQYLLAVDRNNARVAALYDPLHP
HHCCCCCCEEEECCHHHHHHHHHHHHCCEEECCCHHHHHHHHHEECCCCEEEEEECCCCH
AMLRTLLKIVEGGHAAGVEVSICGEMASDPLAVILLIAMGFDTLSMNSASLPRVKWVIRN
HHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
FAIANARKILAEVLEFEHPAEIRFHLQKALEEEGLGGLIRAGKS
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHCCCCC
>Mature Secondary Structure
MLKILKRIVQDVTAASDLKEALGILVQRIDTAINAEAVSVYLIDNKNAEYVLIATEGLNK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEECCCCC
QAEFRVRIALDCGLIGLIGRREEPINIENAPSHPDFYHNPLIGEDHLNAFLGVPIIQHRK
CCEEEEEEEECCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHH
LYGVLVVQQLEQRHFDDAEESFLITLAAQLGGIIAHAEATGELAELTQPKKGIGVDKVEA
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEECCCCCHHHHHCCCCCCCCCCHHHH
AYTALTGIGSVPGIGIGTAVVVYPPADIDAVPRNPVEELEDEINAFYEALETTRDDIRRL
HHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SKRMKETVAEEEHALFDVYLRILDRDSLGAEVEHVIREEKISAQAALATVIKKHVQQFES
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGDDYLRERASDFRDLGRRVLAELQRTQREEIVYPKRTILIGEEITAAALAEVPEGQLAG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHCCCCCEEE
VVSAKGSNNSHVAILARALGVPTVMGLRGLKVELVSRRALIVDGYYGHVYISPSKSLLAE
EEEECCCCCCCCEEHHHHHCCCHHHCCCCCEEEEECCCEEEEECCEEEEEECCCHHHHHH
FKQLAQEEQELNQSLVSLRDKPAETTDNYRVSLQVNTGLAMDAGLSMSVGAEGVGLYRSE
HHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCEEECCCCCEECCCCCCCCHHHC
VPFMSRDRFPSEDEQTIIYRQTLKAFAPRLVTMRTLDIGGDKILPYFPVEEENPYLGWRG
CCCCCCCCCCCCCCCHHHHHHHHHHHCCHHEEEEEEECCCCCCCCCCCCCCCCCCCCEEE
IRITLDHPDVFLIQVRAMMRASEELDNLRIMLPMVTTLSEVEEAVYLIEQAFDELVEEGC
EEEEECCCCEEHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIVKPKIGVMIEVPAAAYLAREMAKRVDFISVGSNDLTQYLLAVDRNNARVAALYDPLHP
HHCCCCCCEEEECCHHHHHHHHHHHHCCEEECCCHHHHHHHHHEECCCCEEEEEECCCCH
AMLRTLLKIVEGGHAAGVEVSICGEMASDPLAVILLIAMGFDTLSMNSASLPRVKWVIRN
HHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
FAIANARKILAEVLEFEHPAEIRFHLQKALEEEGLGGLIRAGKS
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 7896715; 8973315 [H]