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The map label for this gene is rrmJ [H]

Identifier: 54295625

GI number: 54295625

Start: 3096328

End: 3096948

Strand: Reverse

Name: rrmJ [H]

Synonym: lpl2712

Alternate gene names: 54295625

Gene position: 3096948-3096328 (Counterclockwise)

Preceding gene: 54295627

Following gene: 54295624

Centisome position: 92.57

GC content: 39.77

Gene sequence:

>621_bases
ATGAATCGTACTAAAAGCAGTAAACGTTGGCTGCAAGAACATTTTGATGACGTTTATGTAAAAAAAGCGCAGGCTGAAGG
ATATCGAAGTCGAGCCGTATATAAACTCAAAGAAATAGATGACAAAGAGTCACTCATTAAACCAGGCATGACAGTGGTTG
ATTTAGGGGCTTCTCCTGGTGGCTGGACCCAATACGCCTCAGAAAAGATGAAAGGAAGTGGTCGTCTCGTTGCATTAGAT
ATTTTACCCATGGATGCTTTGCCTAATGTGGAGTTTATCCTTGGGGATTTTCGTGAAGATAATGTCTTGCAAGAATTAAT
TAATTTGATTCCTCAAAGGACTTTAGACTTGTTATTATCGGATATGGCCCCAAATATGAGTGGAAGCTCAGCAATAGATA
TTCCAAGAGCCATGTATCTGGTAGAGCTGGCTTTTGATTTCGCGGAAAAAATGTTAAAACCCGGTGGTAATATGCTAGTT
AAAATTTTTCATGGCTCGGGTTTTGATGAATTAGTCAAACAGGCACGAGCGTCTTTTGAAAAAGTGGTAATCCGTAAGCC
ATCCGCTTCAAGATCTCGTTCAAAAGAAACCTATTTGCTGGCAAAGGGCTATAATTTATAG

Upstream 100 bases:

>100_bases
TGATATCCCAGATAATAAAATGGTAAATTATTGCACGTTTTGCTGGGAAAAGGTAGTAAAATTCTGTATCATAGGAAAAA
TTGTTTTATCTTTATTCGTT

Downstream 100 bases:

>100_bases
TAGTTTTATATATATTTATAAATATGCTTGGTGTTGTTTTGGATGATATGAGCTGTGCAAGAGCTATTTTGGGTCTCGCA
ACAAAATTGTAAGTAACTAA

Product: ribosomal RNA large subunit methyltransferase (cell division protein FtsJ)

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase [H]

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MNRTKSSKRWLQEHFDDVYVKKAQAEGYRSRAVYKLKEIDDKESLIKPGMTVVDLGASPGGWTQYASEKMKGSGRLVALD
ILPMDALPNVEFILGDFREDNVLQELINLIPQRTLDLLLSDMAPNMSGSSAIDIPRAMYLVELAFDFAEKMLKPGGNMLV
KIFHGSGFDELVKQARASFEKVVIRKPSASRSRSKETYLLAKGYNL

Sequences:

>Translated_206_residues
MNRTKSSKRWLQEHFDDVYVKKAQAEGYRSRAVYKLKEIDDKESLIKPGMTVVDLGASPGGWTQYASEKMKGSGRLVALD
ILPMDALPNVEFILGDFREDNVLQELINLIPQRTLDLLLSDMAPNMSGSSAIDIPRAMYLVELAFDFAEKMLKPGGNMLV
KIFHGSGFDELVKQARASFEKVVIRKPSASRSRSKETYLLAKGYNL
>Mature_206_residues
MNRTKSSKRWLQEHFDDVYVKKAQAEGYRSRAVYKLKEIDDKESLIKPGMTVVDLGASPGGWTQYASEKMKGSGRLVALD
ILPMDALPNVEFILGDFREDNVLQELINLIPQRTLDLLLSDMAPNMSGSSAIDIPRAMYLVELAFDFAEKMLKPGGNMLV
KIFHGSGFDELVKQARASFEKVVIRKPSASRSRSKETYLLAKGYNL

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit [H]

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family [H]

Homologues:

Organism=Homo sapiens, GI7019377, Length=213, Percent_Identity=34.2723004694836, Blast_Score=144, Evalue=6e-35,
Organism=Homo sapiens, GI29029591, Length=207, Percent_Identity=34.7826086956522, Blast_Score=123, Evalue=9e-29,
Organism=Homo sapiens, GI29029589, Length=207, Percent_Identity=34.7826086956522, Blast_Score=123, Evalue=9e-29,
Organism=Homo sapiens, GI7110661, Length=207, Percent_Identity=34.7826086956522, Blast_Score=123, Evalue=1e-28,
Organism=Homo sapiens, GI194097365, Length=188, Percent_Identity=30.3191489361702, Blast_Score=104, Evalue=5e-23,
Organism=Escherichia coli, GI1789569, Length=202, Percent_Identity=53.4653465346535, Blast_Score=243, Evalue=6e-66,
Organism=Caenorhabditis elegans, GI17554650, Length=208, Percent_Identity=31.25, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17553474, Length=214, Percent_Identity=32.7102803738318, Blast_Score=103, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI71987550, Length=188, Percent_Identity=30.3191489361702, Blast_Score=101, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71987561, Length=188, Percent_Identity=30.3191489361702, Blast_Score=100, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17553860, Length=188, Percent_Identity=30.3191489361702, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6319535, Length=199, Percent_Identity=33.1658291457286, Blast_Score=116, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6319796, Length=189, Percent_Identity=30.6878306878307, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321302, Length=118, Percent_Identity=33.0508474576271, Blast_Score=72, Evalue=7e-14,
Organism=Drosophila melanogaster, GI21356387, Length=211, Percent_Identity=34.5971563981043, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI18859957, Length=188, Percent_Identity=28.7234042553192, Blast_Score=97, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24647580, Length=216, Percent_Identity=27.3148148148148, Blast_Score=93, Evalue=9e-20,
Organism=Drosophila melanogaster, GI24648639, Length=214, Percent_Identity=25.7009345794392, Blast_Score=89, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015507
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: =2.1.1.166 [H]

Molecular weight: Translated: 23136; Mature: 23136

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRTKSSKRWLQEHFDDVYVKKAQAEGYRSRAVYKLKEIDDKESLIKPGMTVVDLGASPG
CCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHCCCCEEEECCCCCC
GWTQYASEKMKGSGRLVALDILPMDALPNVEFILGDFREDNVLQELINLIPQRTLDLLLS
CHHHHHHHHHCCCCCEEEEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHH
DMAPNMSGSSAIDIPRAMYLVELAFDFAEKMLKPGGNMLVKIFHGSGFDELVKQARASFE
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHH
KVVIRKPSASRSRSKETYLLAKGYNL
HHEEECCCCCCCCCCCEEEEECCCCC
>Mature Secondary Structure
MNRTKSSKRWLQEHFDDVYVKKAQAEGYRSRAVYKLKEIDDKESLIKPGMTVVDLGASPG
CCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHCCCCEEEECCCCCC
GWTQYASEKMKGSGRLVALDILPMDALPNVEFILGDFREDNVLQELINLIPQRTLDLLLS
CHHHHHHHHHCCCCCEEEEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHH
DMAPNMSGSSAIDIPRAMYLVELAFDFAEKMLKPGGNMLVKIFHGSGFDELVKQARASFE
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHH
KVVIRKPSASRSRSKETYLLAKGYNL
HHEEECCCCCCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA