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The map label for this gene is nuoB

Identifier: 54295617

GI number: 54295617

Start: 3085965

End: 3086441

Strand: Reverse

Name: nuoB

Synonym: lpl2704

Alternate gene names: 54295617

Gene position: 3086441-3085965 (Counterclockwise)

Preceding gene: 54295618

Following gene: 54295616

Centisome position: 92.25

GC content: 46.96

Gene sequence:

>477_bases
ATGGCGGTTGCAGAGCTAGAAAAAAAAGGTTTTGAGTTGACTACGGTCGAAAAATTGGTTGGTTGGGCACGCAGTGGCTC
CATGTGGCCAATGACATTTGGCTTGGCTTGCTGTGCCGTGGAAATGATGCACGTGGGAGCAGCACGCTATGATTTGGACA
GGTTTGGTATTATTTTCCGCCCCAGTCCACGCCAGTCAGATGTCATGATTGTAGCAGGCACTTTATGTAACAAAATGGCC
CCGGCACTCAGAAAAGTTTATGATCAGATGCCAGAACCACGCTGGGTTATTTCAATGGGGTCTTGTGCCAATGGTGGGGG
ATACTATCATTATTCCTATTCAGTGGTTCGTGGCTGCGATCGTATTGTGCCAGTCGATGTCTATGTTCCTGGCTGTCCAC
CAACAGCAGAAGCGTTGTTATATGGCATTATTCAACTACAGAATAAAATCAGGCGGAAACCTGTTTTAGAGGCATAA

Upstream 100 bases:

>100_bases
CATCCTTTGCTAAAACGATAGCTGAGATTATATTTTAAGCCATTGTAATAATGGTTATGTAAACTGGATTATTAATTATT
TAACTGCTTAGAGGCGAACT

Downstream 100 bases:

>100_bases
GCTCATTAAGGGTGCAGATTAGATGACAAAAAATGAGTATTTGATTGAAAAGCTACAAGTTGATTTAGCAAATCATATTA
CCGAGTTAACCTCCGCATAT

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MAVAELEKKGFELTTVEKLVGWARSGSMWPMTFGLACCAVEMMHVGAARYDLDRFGIIFRPSPRQSDVMIVAGTLCNKMA
PALRKVYDQMPEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALLYGIIQLQNKIRRKPVLEA

Sequences:

>Translated_158_residues
MAVAELEKKGFELTTVEKLVGWARSGSMWPMTFGLACCAVEMMHVGAARYDLDRFGIIFRPSPRQSDVMIVAGTLCNKMA
PALRKVYDQMPEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALLYGIIQLQNKIRRKPVLEA
>Mature_157_residues
AVAELEKKGFELTTVEKLVGWARSGSMWPMTFGLACCAVEMMHVGAARYDLDRFGIIFRPSPRQSDVMIVAGTLCNKMAP
ALRKVYDQMPEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALLYGIIQLQNKIRRKPVLEA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=147, Percent_Identity=78.9115646258503, Blast_Score=258, Evalue=2e-69,
Organism=Escherichia coli, GI1788624, Length=154, Percent_Identity=50.6493506493506, Blast_Score=161, Evalue=2e-41,
Organism=Escherichia coli, GI1789074, Length=111, Percent_Identity=45.045045045045, Blast_Score=102, Evalue=1e-23,
Organism=Escherichia coli, GI1788834, Length=122, Percent_Identity=40.1639344262295, Blast_Score=91, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17509685, Length=147, Percent_Identity=69.3877551020408, Blast_Score=241, Evalue=8e-65,
Organism=Drosophila melanogaster, GI18859983, Length=147, Percent_Identity=75.5102040816327, Blast_Score=259, Evalue=5e-70,
Organism=Drosophila melanogaster, GI24642371, Length=147, Percent_Identity=75.5102040816327, Blast_Score=259, Evalue=5e-70,
Organism=Drosophila melanogaster, GI24651058, Length=142, Percent_Identity=77.4647887323944, Blast_Score=251, Evalue=1e-67,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_LEGPA (Q5X1A8)

Other databases:

- EMBL:   CR628336
- RefSeq:   YP_125140.1
- HSSP:   Q56218
- ProteinModelPortal:   Q5X1A8
- SMR:   Q5X1A8
- STRING:   Q5X1A8
- GeneID:   3119391
- GenomeReviews:   CR628336_GR
- KEGG:   lpp:lpp2835
- LegioList:   lpp2835
- eggNOG:   COG0377
- HOGENOM:   HBG553221
- OMA:   GKPIGSN
- ProtClustDB:   PRK06411
- BioCyc:   LPNE297246:LPP2835-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 17539; Mature: 17408

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
9.5 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
8.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVAELEKKGFELTTVEKLVGWARSGSMWPMTFGLACCAVEMMHVGAARYDLDRFGIIFR
CCCCHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCHHHCCHHHCCEEEC
PSPRQSDVMIVAGTLCNKMAPALRKVYDQMPEPRWVISMGSCANGGGYYHYSYSVVRGCD
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEHHHHHCCC
RIVPVDVYVPGCPPTAEALLYGIIQLQNKIRRKPVLEA
CEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AVAELEKKGFELTTVEKLVGWARSGSMWPMTFGLACCAVEMMHVGAARYDLDRFGIIFR
CCCHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCHHHCCHHHCCEEEC
PSPRQSDVMIVAGTLCNKMAPALRKVYDQMPEPRWVISMGSCANGGGYYHYSYSVVRGCD
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEHHHHHCCC
RIVPVDVYVPGCPPTAEALLYGIIQLQNKIRRKPVLEA
CEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA