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The map label for this gene is truB [H]

Identifier: 54295600

GI number: 54295600

Start: 3066085

End: 3066996

Strand: Reverse

Name: truB [H]

Synonym: lpl2687

Alternate gene names: 54295600

Gene position: 3066996-3066085 (Counterclockwise)

Preceding gene: 54295601

Following gene: 54295599

Centisome position: 91.67

GC content: 39.25

Gene sequence:

>912_bases
ATGACAACAATTGAATCGAAGTGTTCCATAGATGGAATCCTGCTATTAAATAAACCACAAGGAATGACCTCTAACGCCGC
TCTTCAGAAAGCCAAACATTTGTTTGGTGCTAAAAAAGCTGGTCACACAGGCAGCTTGGATCCTTTAGCAACCGGCATGT
TACCTCTATGCTTCGGAGAAGCGACTAAAATATGTCAGTATCTCCTTAATGCGGATAAAAGCTATGAAACAATTGGAAGG
CTGGGCAGCAAGACAAATACTGCTGATTGTACTGGCGAAGTGATATTTTGCATTGAAAATTATACTGTTAGCCATGAGGA
ATTGATTGCAACCCTAGAAAAATATAAGGGTAAAACCAAGCAAATTCCATCCATGTTTTCTGCTTTAAAGCACAAAGGAA
CCCCTTTGTATCGACTTGCCAGAGAAGGTATAGAGATAGAACGTAAGGCCAGGGATATAGTAATCAGCCAATTGAAGTTG
GAACAATTTGATGGTGAATGTTTTACATTAACGGTATCATGCAGCAAAGGAACCTATATACGTAATTTAGTGGAAGATAT
TGGTGATACGTTGAAGGCAGGAGCTCATATGACAAAATTGCATAGGCTCTACACTGCAGGGTTTGAAAATAATCGAATGT
ACACTCTCGATGAATTACAGGATATGCCTTTATCACAAAGGCTTGCTTGCTTAATCCCTATTGATCAGGCCGTTCAACAT
TTAACACCAGTAATTCTTTCAGACTCTGAGGTAACAGCAATTCGCCAGGGCAAGGTAATTTCAAATAAAACTAGTGCTGT
TGAAGGAGAAGATTTGCGCCTTTATGGCGAGCAATCCCAATTTATTGGCATTGGCCAAGCTTTAATTCATGGTGATATTA
AAGCAAAAAGGTTAGTTTCTTTTGCGTTGTAA

Upstream 100 bases:

>100_bases
ACTGTGCCGCAACTTCATTTTATTTATGATGAATCGATTGAGTATGGACAACGGTTAAGCCGATTAATTGATGAAGTAAA
TCCCCCTGATTCAAGTAGTG

Downstream 100 bases:

>100_bases
TTACAATTTTTGTAAGAATTGCCGCCTACTTTGTAATAAGTATGAAATTTTTTACACAAATTTATATCCACTACTTGTTT
CATTTGACATGCCTTGGTAG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MTTIESKCSIDGILLLNKPQGMTSNAALQKAKHLFGAKKAGHTGSLDPLATGMLPLCFGEATKICQYLLNADKSYETIGR
LGSKTNTADCTGEVIFCIENYTVSHEELIATLEKYKGKTKQIPSMFSALKHKGTPLYRLAREGIEIERKARDIVISQLKL
EQFDGECFTLTVSCSKGTYIRNLVEDIGDTLKAGAHMTKLHRLYTAGFENNRMYTLDELQDMPLSQRLACLIPIDQAVQH
LTPVILSDSEVTAIRQGKVISNKTSAVEGEDLRLYGEQSQFIGIGQALIHGDIKAKRLVSFAL

Sequences:

>Translated_303_residues
MTTIESKCSIDGILLLNKPQGMTSNAALQKAKHLFGAKKAGHTGSLDPLATGMLPLCFGEATKICQYLLNADKSYETIGR
LGSKTNTADCTGEVIFCIENYTVSHEELIATLEKYKGKTKQIPSMFSALKHKGTPLYRLAREGIEIERKARDIVISQLKL
EQFDGECFTLTVSCSKGTYIRNLVEDIGDTLKAGAHMTKLHRLYTAGFENNRMYTLDELQDMPLSQRLACLIPIDQAVQH
LTPVILSDSEVTAIRQGKVISNKTSAVEGEDLRLYGEQSQFIGIGQALIHGDIKAKRLVSFAL
>Mature_302_residues
TTIESKCSIDGILLLNKPQGMTSNAALQKAKHLFGAKKAGHTGSLDPLATGMLPLCFGEATKICQYLLNADKSYETIGRL
GSKTNTADCTGEVIFCIENYTVSHEELIATLEKYKGKTKQIPSMFSALKHKGTPLYRLAREGIEIERKARDIVISQLKLE
QFDGECFTLTVSCSKGTYIRNLVEDIGDTLKAGAHMTKLHRLYTAGFENNRMYTLDELQDMPLSQRLACLIPIDQAVQHL
TPVILSDSEVTAIRQGKVISNKTSAVEGEDLRLYGEQSQFIGIGQALIHGDIKAKRLVSFAL

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=259, Percent_Identity=31.6602316602317, Blast_Score=118, Evalue=6e-27,
Organism=Homo sapiens, GI215599015, Length=260, Percent_Identity=26.5384615384615, Blast_Score=65, Evalue=6e-11,
Organism=Homo sapiens, GI4503337, Length=260, Percent_Identity=26.5384615384615, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI2367200, Length=300, Percent_Identity=43, Blast_Score=250, Evalue=8e-68,
Organism=Saccharomyces cerevisiae, GI6324037, Length=191, Percent_Identity=30.3664921465969, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323204, Length=257, Percent_Identity=26.0700389105058, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI281364189, Length=264, Percent_Identity=24.6212121212121, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281364187, Length=264, Percent_Identity=24.6212121212121, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281364185, Length=264, Percent_Identity=24.6212121212121, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281364183, Length=264, Percent_Identity=24.6212121212121, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI62471759, Length=264, Percent_Identity=24.6212121212121, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI17975520, Length=264, Percent_Identity=24.6212121212121, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240 [H]

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 33359; Mature: 33228

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTIESKCSIDGILLLNKPQGMTSNAALQKAKHLFGAKKAGHTGSLDPLATGMLPLCFGE
CCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHHHHH
ATKICQYLLNADKSYETIGRLGSKTNTADCTGEVIFCIENYTVSHEELIATLEKYKGKTK
HHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCHH
QIPSMFSALKHKGTPLYRLAREGIEIERKARDIVISQLKLEQFDGECFTLTVSCSKGTYI
HHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHH
RNLVEDIGDTLKAGAHMTKLHRLYTAGFENNRMYTLDELQDMPLSQRLACLIPIDQAVQH
HHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCEEEEHHHHHCCCHHHHHEEEECHHHHHHH
LTPVILSDSEVTAIRQGKVISNKTSAVEGEDLRLYGEQSQFIGIGQALIHGDIKAKRLVS
HCCEEECCCCEEHHHCCCEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCHHHHHHHH
FAL
HCC
>Mature Secondary Structure 
TTIESKCSIDGILLLNKPQGMTSNAALQKAKHLFGAKKAGHTGSLDPLATGMLPLCFGE
CCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHHHHH
ATKICQYLLNADKSYETIGRLGSKTNTADCTGEVIFCIENYTVSHEELIATLEKYKGKTK
HHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCHH
QIPSMFSALKHKGTPLYRLAREGIEIERKARDIVISQLKLEQFDGECFTLTVSCSKGTYI
HHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHH
RNLVEDIGDTLKAGAHMTKLHRLYTAGFENNRMYTLDELQDMPLSQRLACLIPIDQAVQH
HHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCEEEEHHHHHCCCHHHHHEEEECHHHHHHH
LTPVILSDSEVTAIRQGKVISNKTSAVEGEDLRLYGEQSQFIGIGQALIHGDIKAKRLVS
HCCEEECCCCEEHHHCCCEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCHHHHHHHH
FAL
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA