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The map label for this gene is topA [H]

Identifier: 54295436

GI number: 54295436

Start: 2875877

End: 2878156

Strand: Direct

Name: topA [H]

Synonym: lpl2522

Alternate gene names: 54295436

Gene position: 2875877-2878156 (Clockwise)

Preceding gene: 54295435

Following gene: 54295439

Centisome position: 85.96

GC content: 41.84

Gene sequence:

>2280_bases
ATGAGTAAACACTTGGTGATCGTAGAGTCGCCCGCGAAAGCTAAAACAATACAGAAATATTTAGGAAATGACTATGACGT
GTTAGCGTCTTATGGTCATGTGCGTGATCTGCCTGCTCGTAAAGGGTCAGTAAACCCTGAAAAACATTTTTCCATGACTT
ATGTTCCTATTGAAAAAAATGCGAGACACATTGATACGATAGCAAAAACTCTTAAAAAATCAGACTCTCTACTGCTGGCA
ACTGACCCTGATAGAGAGGGAGAAGCTATCTCGTGGCATATTTTTGAATTAATGAAAGAACGAAACCTTTTAAAGGACAA
ATCAGTTCATCGAATCTTTTTTAATGAAATTACAAAAGCAGCAATCCAGGATGCAATTAATCATCCTCGCTCCATATCCA
TGGATTTGGTGAATGCTCAACAAGCCCGACGCGCTCTTGATTATCTGGTTGGCTTTAACCTGTCCCCTTTGCTATGGAAA
AAGATTCGGCGGGGATTATCGGCCGGACGAGTACAAAGCCCTGCCCTTAGGTTAATTGTTGAGCGAGAAGAGGAAATTGA
ACGTTTCATTGCTCAGGAATACTGGAAAATAATCGCAAAATGCGCTCATGCCAGCACTGAATTTGAAGCACGACTGACCC
ATTATAACGAAGAGAAACTTCAACAGTTTTCAGTCACTCAACAAGAACAAGCCCATGAAATAAAAAAGCAACTGATAGCA
CAGGCACAAGGCTTTTTAACCGTCGCTCAAATTGATAAAAAACAGCGTAAACGTAAACCATCCCCCCCCTTCATTACCTC
TACCTTGCAACAGGAAGCCGCCAGAAAATTAGGTTTCACCGCACGAAAAACCATGATGGTCGCTCAGCAATTATATGAAG
GTATTGATATAGGCACAGGAACCGTGGGTTTAATCACTTATATGCGTACTGACTCAGTCAACCTGGCAAAGGAAGCGATT
GACGAAATACGCGACTATATCACACAACGTTATAAGGGAGATAATTGCCCGAATTCTCCCAGAATTTACAAAACTAAATC
CAAAAACGCACAGGAAGCACATGAAGCCATTAGACCAACTTCCATCAAAAGAACTCCAGAAATGGTGCAAGGTTCCCTGA
CAAGCGATCAATTGAAACTCTATAGCTTAATCTGGAAACGCACAGTGGCCTCACAGATGGCTGATGCCATCCTGGATACG
GTGAGCGTGGATTTTAGCTGTGGTAAAGGGAATACCTTCAGAGCCAATGGTTCAACAATAGCCTTTCCTGGCTTCCTTTC
TGTCTATGAAGAAGGACGAGATGATTCTAAAGACGAAGACAATGAAGATAAAATTTTACCTGCTTTCAATGTAGGTGAAA
AAATAAAAGTATCGGATATTGAAACCAATCAGCATTTTACCGAGCCACCTCCGAGATATTCTGAAGCAACTTTGGTTAAA
GCTCTTGAAGAATACGATATAGGCAGACCGTCTACTTATGCCTCTATCATTCATACTTTGCAGCAACGCGAATACGTGGT
AGTTGAAAAAAAACGTTTCTTGCCCACTGACGTGGGTAGAATTGTCAATCGATTTCTAACCAACTATTTTACTCGCTATG
TCGACTATCAGTTTACCGCTGGCCTTGAAGACACACTTGATGCCATCGCGCGTGGAGAAAAGGATTGGATCCCTGTTCTG
GAAGAGTTCTGGCAACCTTTCGTCCAGCAAATTCAGAATATAGATGAACAAGTGCAAAGAAAGGATGTTACTACGGAATT
ATTAGATGAAAAATGTCCTAAATGTCAAAAACCATTGTCCATTAGGCTCGGCAAGAGAGGGCGCTTTATTGGCTGCACAG
GATATCCGGATTGCGATTACACTCAGGATATAAGCAATCCAGAAGGAGAAAAATCAGAACCTGAAGTCGTGGAAGGCAGA
AGCTGCCCTCTGTGTCATGGAGCCTTACATATTAAAACCGGTCGCTATGGAAAATTTATTGGATGCAGCAATTATCCTGA
ATGCAAGCATATGGAACCTTTAGAAAAACCCTCCGATACTGGCGTCACATGCCCTAAATGCAGCGAGGCAAAAATTCTGC
AGCGGAAATCAAGAAAAGGGAAAATCTTTTATTCCTGCGGAAATTACCCAAAATGCGATTACGCTCTGTGGAATGAACCC
GTTGATTTGCCTTGTCCTAAATGTGCATGGCCAATCTTAACCGTAAAAGAAAGTAAAAAATTTGGTCGTCAAATTCTTTG
TCCTCGTGAAGGTTGTGATTACTCTGCCAAGGAAGACTAG

Upstream 100 bases:

>100_bases
AGATGAATTAACAATTCACTAATTAATGAGTGTACTGATATCAACCCATTCATGAACTGTTAGTGAGCCACACAACCCAA
AAGGATAAGGTTTATTTGAT

Downstream 100 bases:

>100_bases
GTACTTTGTTACCCCGGGGCCTACGCCCAAGCCTTTCAGACTATTGGATAGCGAATAATATGTCTTTTTTATTCGCTATC
CATTAATCCAGCAAATTATC

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 759; Mature: 758

Protein sequence:

>759_residues
MSKHLVIVESPAKAKTIQKYLGNDYDVLASYGHVRDLPARKGSVNPEKHFSMTYVPIEKNARHIDTIAKTLKKSDSLLLA
TDPDREGEAISWHIFELMKERNLLKDKSVHRIFFNEITKAAIQDAINHPRSISMDLVNAQQARRALDYLVGFNLSPLLWK
KIRRGLSAGRVQSPALRLIVEREEEIERFIAQEYWKIIAKCAHASTEFEARLTHYNEEKLQQFSVTQQEQAHEIKKQLIA
QAQGFLTVAQIDKKQRKRKPSPPFITSTLQQEAARKLGFTARKTMMVAQQLYEGIDIGTGTVGLITYMRTDSVNLAKEAI
DEIRDYITQRYKGDNCPNSPRIYKTKSKNAQEAHEAIRPTSIKRTPEMVQGSLTSDQLKLYSLIWKRTVASQMADAILDT
VSVDFSCGKGNTFRANGSTIAFPGFLSVYEEGRDDSKDEDNEDKILPAFNVGEKIKVSDIETNQHFTEPPPRYSEATLVK
ALEEYDIGRPSTYASIIHTLQQREYVVVEKKRFLPTDVGRIVNRFLTNYFTRYVDYQFTAGLEDTLDAIARGEKDWIPVL
EEFWQPFVQQIQNIDEQVQRKDVTTELLDEKCPKCQKPLSIRLGKRGRFIGCTGYPDCDYTQDISNPEGEKSEPEVVEGR
SCPLCHGALHIKTGRYGKFIGCSNYPECKHMEPLEKPSDTGVTCPKCSEAKILQRKSRKGKIFYSCGNYPKCDYALWNEP
VDLPCPKCAWPILTVKESKKFGRQILCPREGCDYSAKED

Sequences:

>Translated_759_residues
MSKHLVIVESPAKAKTIQKYLGNDYDVLASYGHVRDLPARKGSVNPEKHFSMTYVPIEKNARHIDTIAKTLKKSDSLLLA
TDPDREGEAISWHIFELMKERNLLKDKSVHRIFFNEITKAAIQDAINHPRSISMDLVNAQQARRALDYLVGFNLSPLLWK
KIRRGLSAGRVQSPALRLIVEREEEIERFIAQEYWKIIAKCAHASTEFEARLTHYNEEKLQQFSVTQQEQAHEIKKQLIA
QAQGFLTVAQIDKKQRKRKPSPPFITSTLQQEAARKLGFTARKTMMVAQQLYEGIDIGTGTVGLITYMRTDSVNLAKEAI
DEIRDYITQRYKGDNCPNSPRIYKTKSKNAQEAHEAIRPTSIKRTPEMVQGSLTSDQLKLYSLIWKRTVASQMADAILDT
VSVDFSCGKGNTFRANGSTIAFPGFLSVYEEGRDDSKDEDNEDKILPAFNVGEKIKVSDIETNQHFTEPPPRYSEATLVK
ALEEYDIGRPSTYASIIHTLQQREYVVVEKKRFLPTDVGRIVNRFLTNYFTRYVDYQFTAGLEDTLDAIARGEKDWIPVL
EEFWQPFVQQIQNIDEQVQRKDVTTELLDEKCPKCQKPLSIRLGKRGRFIGCTGYPDCDYTQDISNPEGEKSEPEVVEGR
SCPLCHGALHIKTGRYGKFIGCSNYPECKHMEPLEKPSDTGVTCPKCSEAKILQRKSRKGKIFYSCGNYPKCDYALWNEP
VDLPCPKCAWPILTVKESKKFGRQILCPREGCDYSAKED
>Mature_758_residues
SKHLVIVESPAKAKTIQKYLGNDYDVLASYGHVRDLPARKGSVNPEKHFSMTYVPIEKNARHIDTIAKTLKKSDSLLLAT
DPDREGEAISWHIFELMKERNLLKDKSVHRIFFNEITKAAIQDAINHPRSISMDLVNAQQARRALDYLVGFNLSPLLWKK
IRRGLSAGRVQSPALRLIVEREEEIERFIAQEYWKIIAKCAHASTEFEARLTHYNEEKLQQFSVTQQEQAHEIKKQLIAQ
AQGFLTVAQIDKKQRKRKPSPPFITSTLQQEAARKLGFTARKTMMVAQQLYEGIDIGTGTVGLITYMRTDSVNLAKEAID
EIRDYITQRYKGDNCPNSPRIYKTKSKNAQEAHEAIRPTSIKRTPEMVQGSLTSDQLKLYSLIWKRTVASQMADAILDTV
SVDFSCGKGNTFRANGSTIAFPGFLSVYEEGRDDSKDEDNEDKILPAFNVGEKIKVSDIETNQHFTEPPPRYSEATLVKA
LEEYDIGRPSTYASIIHTLQQREYVVVEKKRFLPTDVGRIVNRFLTNYFTRYVDYQFTAGLEDTLDAIARGEKDWIPVLE
EFWQPFVQQIQNIDEQVQRKDVTTELLDEKCPKCQKPLSIRLGKRGRFIGCTGYPDCDYTQDISNPEGEKSEPEVVEGRS
CPLCHGALHIKTGRYGKFIGCSNYPECKHMEPLEKPSDTGVTCPKCSEAKILQRKSRKGKIFYSCGNYPKCDYALWNEPV
DLPCPKCAWPILTVKESKKFGRQILCPREGCDYSAKED

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=643, Percent_Identity=25.6609642301711, Blast_Score=148, Evalue=2e-35,
Organism=Homo sapiens, GI4507635, Length=562, Percent_Identity=23.8434163701068, Blast_Score=117, Evalue=5e-26,
Organism=Escherichia coli, GI1787529, Length=816, Percent_Identity=36.8872549019608, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI48994931, Length=165, Percent_Identity=43.6363636363636, Blast_Score=151, Evalue=2e-37,
Organism=Escherichia coli, GI1788061, Length=598, Percent_Identity=25.5852842809365, Blast_Score=141, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI32563869, Length=695, Percent_Identity=24.3165467625899, Blast_Score=135, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI17555378, Length=531, Percent_Identity=25.2354048964218, Blast_Score=122, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6323263, Length=496, Percent_Identity=23.5887096774194, Blast_Score=104, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24585251, Length=636, Percent_Identity=24.8427672955975, Blast_Score=152, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24640096, Length=575, Percent_Identity=22.6086956521739, Blast_Score=102, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 86545; Mature: 86413

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKHLVIVESPAKAKTIQKYLGNDYDVLASYGHVRDLPARKGSVNPEKHFSMTYVPIEKN
CCCCEEEEECCHHHHHHHHHHCCCHHHHHCCCCHHCCCCCCCCCCCCCCCEEEEEECCCC
ARHIDTIAKTLKKSDSLLLATDPDREGEAISWHIFELMKERNLLKDKSVHRIFFNEITKA
CHHHHHHHHHHHCCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIQDAINHPRSISMDLVNAQQARRALDYLVGFNLSPLLWKKIRRGLSAGRVQSPALRLIV
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCHHHHHH
EREEEIERFIAQEYWKIIAKCAHASTEFEARLTHYNEEKLQQFSVTQQEQAHEIKKQLIA
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
QAQGFLTVAQIDKKQRKRKPSPPFITSTLQQEAARKLGFTARKTMMVAQQLYEGIDIGTG
HHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCC
TVGLITYMRTDSVNLAKEAIDEIRDYITQRYKGDNCPNSPRIYKTKSKNAQEAHEAIRPT
HHHHHEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHCCC
SIKRTPEMVQGSLTSDQLKLYSLIWKRTVASQMADAILDTVSVDFSCGKGNTFRANGSTI
CCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEECCCCEE
AFPGFLSVYEEGRDDSKDEDNEDKILPAFNVGEKIKVSDIETNQHFTEPPPRYSEATLVK
ECCHHHHHHHCCCCCCCCCCCCCCEEECCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHH
ALEEYDIGRPSTYASIIHTLQQREYVVVEKKRFLPTDVGRIVNRFLTNYFTRYVDYQFTA
HHHHHCCCCCHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
GLEDTLDAIARGEKDWIPVLEEFWQPFVQQIQNIDEQVQRKDVTTELLDEKCPKCQKPLS
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
IRLGKRGRFIGCTGYPDCDYTQDISNPEGEKSEPEVVEGRSCPLCHGALHIKTGRYGKFI
EEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCEEEEECCCCCCEE
GCSNYPECKHMEPLEKPSDTGVTCPKCSEAKILQRKSRKGKIFYSCGNYPKCDYALWNEP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCHHHCCCC
VDLPCPKCAWPILTVKESKKFGRQILCPREGCDYSAKED
CCCCCCCCCCCCEEEHHHHHHCCEECCCCCCCCCCCCCC
>Mature Secondary Structure 
SKHLVIVESPAKAKTIQKYLGNDYDVLASYGHVRDLPARKGSVNPEKHFSMTYVPIEKN
CCCEEEEECCHHHHHHHHHHCCCHHHHHCCCCHHCCCCCCCCCCCCCCCEEEEEECCCC
ARHIDTIAKTLKKSDSLLLATDPDREGEAISWHIFELMKERNLLKDKSVHRIFFNEITKA
CHHHHHHHHHHHCCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIQDAINHPRSISMDLVNAQQARRALDYLVGFNLSPLLWKKIRRGLSAGRVQSPALRLIV
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCHHHHHH
EREEEIERFIAQEYWKIIAKCAHASTEFEARLTHYNEEKLQQFSVTQQEQAHEIKKQLIA
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
QAQGFLTVAQIDKKQRKRKPSPPFITSTLQQEAARKLGFTARKTMMVAQQLYEGIDIGTG
HHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCC
TVGLITYMRTDSVNLAKEAIDEIRDYITQRYKGDNCPNSPRIYKTKSKNAQEAHEAIRPT
HHHHHEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHCCC
SIKRTPEMVQGSLTSDQLKLYSLIWKRTVASQMADAILDTVSVDFSCGKGNTFRANGSTI
CCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEECCCCEE
AFPGFLSVYEEGRDDSKDEDNEDKILPAFNVGEKIKVSDIETNQHFTEPPPRYSEATLVK
ECCHHHHHHHCCCCCCCCCCCCCCEEECCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHH
ALEEYDIGRPSTYASIIHTLQQREYVVVEKKRFLPTDVGRIVNRFLTNYFTRYVDYQFTA
HHHHHCCCCCHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
GLEDTLDAIARGEKDWIPVLEEFWQPFVQQIQNIDEQVQRKDVTTELLDEKCPKCQKPLS
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
IRLGKRGRFIGCTGYPDCDYTQDISNPEGEKSEPEVVEGRSCPLCHGALHIKTGRYGKFI
EEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCEEEEECCCCCCEE
GCSNYPECKHMEPLEKPSDTGVTCPKCSEAKILQRKSRKGKIFYSCGNYPKCDYALWNEP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCHHHCCCC
VDLPCPKCAWPILTVKESKKFGRQILCPREGCDYSAKED
CCCCCCCCCCCCEEEHHHHHHCCEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]