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The map label for this gene is lcfB [H]

Identifier: 54295075

GI number: 54295075

Start: 2463708

End: 2465087

Strand: Direct

Name: lcfB [H]

Synonym: lpl2155

Alternate gene names: 54295075

Gene position: 2463708-2465087 (Clockwise)

Preceding gene: 54295074

Following gene: 54295076

Centisome position: 73.64

GC content: 38.91

Gene sequence:

>1380_bases
ATGCTGTTTTTGGCATCAGATGTTCCAAACAAAGTAGCAATTACCCATGGCAATCGAGTAATAACTTTTGATGAATTAAC
ACATGAAGTTTTAGCAAGTTCAAAGCAATTCCTGAAACTTCCAAAAACCATCATTGTCCTCCATGCAACACCTGAGATTG
GATTTATTATTCAACTATTAGCCTGTTTGGAAACTGGTCGCCCCATTGCCCTTTTCCCCAATTCCATTTCAGAGGAAGAA
AAGCAAAGACGACTATCTCTTTTGGGTAATGCCGTAATGATAGATGAAGAAGGAGAATTGCAGGAAATATTTGAAAATAA
AACGATCGAGCCCCATCCCCAGACAGCACTTATTCTCTTCACCTCAGGAAGCACAGGAAAAGTTAAAGCAGTTCAACTAT
CAAGCATCAATATAAAAGCCAACTGCCATGCGGTCATAAAAGCGCTGGAATTTAAAAAAATTCAAGATCAATTGCTCTTT
TTACCTTTATCCTATTCTTTTGGCCTTCTGGGCCAATTGCTCCCTGGTTTAATGTTTGGCCTGACCACTCGACTAATCAC
TCATTTTACAGACATTAAAATGCTAATGGAGCAAAGTACCGTTCCTCAAATGTGGAGTGGCGTACCTTCTCACTGGGTAG
CTATCAATGCCATAGCAAAACGATACCCTGATAGCGCAGCCAAAATTAACGCGATTGTTTCTGCTGGCGCTCCTTTATCC
ACTGCACTCAGAACGGATTTGAAACAAATATTTCCTAACGCTATCATCTATAACAATTACGGCTTAACTGAAGCGTCTCC
GAGAGTACTGACTTATTCCAGTAAGGATCCTTTATTCACAGAAAATTATGCGGGTTATCCTGTTGGCGATTGGAAGGTCA
AACTTTCTGCTGAACATGAATTATTAATTAGCGGCACTCAAATGATGCTGGGTTATCTTGGTGAAAAGGATAGTACTAAA
ATACAAAATGGCTGGTTATCAACAGGAGATATTGCAGAAATATTACCCAATGGGCTAGTCGCAATCAAAGGCCGTCGAGA
CAGCCTTGTTAATATTGGGGGAGAAAAAGTTAACCTCTCTGAGATCGAGGAAAAAATTTGCCAAATTGATGTGATAAAAG
AGGCCATTGTTATCCCTCAGGAAGACAAGATATATGGGACACGATTAATTATCTGTATCGAAAAAAAATCGCTGCCTTCT
TCCATTTCTGAACAAAATTTAATAGAAAAAATACAAACGCATCTACTACCCAGGAAATTGCCCATTCAATTACAGCTCAT
GTCATCCCTACCCAGAAATCAACATGGGAAGCTTGATAGAAAAGCGTTGGTTTTGAATATTGATGGTTTAGAGAATAAGG
AAAAAAATAATGCTAAGTAA

Upstream 100 bases:

>100_bases
AGCGGAAAAATTCGCAGGAATTTTTGCCGAAAACATCTTTTGGATAAAACTCTGCCAATAGTGGCTACCTGGCAACTCAA
TAAAATTGAGGAATAAAATC

Downstream 100 bases:

>100_bases
TCGAATTGAACAAGTACTGGAACGAATTGGTCTTACCAATCTTCCTGAAAACAAACAGGCAAAACTTGAGCAAGGGGGCT
TGGATAGTTTGATGCTGGCT

Product: hypothetical protein

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MLFLASDVPNKVAITHGNRVITFDELTHEVLASSKQFLKLPKTIIVLHATPEIGFIIQLLACLETGRPIALFPNSISEEE
KQRRLSLLGNAVMIDEEGELQEIFENKTIEPHPQTALILFTSGSTGKVKAVQLSSINIKANCHAVIKALEFKKIQDQLLF
LPLSYSFGLLGQLLPGLMFGLTTRLITHFTDIKMLMEQSTVPQMWSGVPSHWVAINAIAKRYPDSAAKINAIVSAGAPLS
TALRTDLKQIFPNAIIYNNYGLTEASPRVLTYSSKDPLFTENYAGYPVGDWKVKLSAEHELLISGTQMMLGYLGEKDSTK
IQNGWLSTGDIAEILPNGLVAIKGRRDSLVNIGGEKVNLSEIEEKICQIDVIKEAIVIPQEDKIYGTRLIICIEKKSLPS
SISEQNLIEKIQTHLLPRKLPIQLQLMSSLPRNQHGKLDRKALVLNIDGLENKEKNNAK

Sequences:

>Translated_459_residues
MLFLASDVPNKVAITHGNRVITFDELTHEVLASSKQFLKLPKTIIVLHATPEIGFIIQLLACLETGRPIALFPNSISEEE
KQRRLSLLGNAVMIDEEGELQEIFENKTIEPHPQTALILFTSGSTGKVKAVQLSSINIKANCHAVIKALEFKKIQDQLLF
LPLSYSFGLLGQLLPGLMFGLTTRLITHFTDIKMLMEQSTVPQMWSGVPSHWVAINAIAKRYPDSAAKINAIVSAGAPLS
TALRTDLKQIFPNAIIYNNYGLTEASPRVLTYSSKDPLFTENYAGYPVGDWKVKLSAEHELLISGTQMMLGYLGEKDSTK
IQNGWLSTGDIAEILPNGLVAIKGRRDSLVNIGGEKVNLSEIEEKICQIDVIKEAIVIPQEDKIYGTRLIICIEKKSLPS
SISEQNLIEKIQTHLLPRKLPIQLQLMSSLPRNQHGKLDRKALVLNIDGLENKEKNNAK
>Mature_459_residues
MLFLASDVPNKVAITHGNRVITFDELTHEVLASSKQFLKLPKTIIVLHATPEIGFIIQLLACLETGRPIALFPNSISEEE
KQRRLSLLGNAVMIDEEGELQEIFENKTIEPHPQTALILFTSGSTGKVKAVQLSSINIKANCHAVIKALEFKKIQDQLLF
LPLSYSFGLLGQLLPGLMFGLTTRLITHFTDIKMLMEQSTVPQMWSGVPSHWVAINAIAKRYPDSAAKINAIVSAGAPLS
TALRTDLKQIFPNAIIYNNYGLTEASPRVLTYSSKDPLFTENYAGYPVGDWKVKLSAEHELLISGTQMMLGYLGEKDSTK
IQNGWLSTGDIAEILPNGLVAIKGRRDSLVNIGGEKVNLSEIEEKICQIDVIKEAIVIPQEDKIYGTRLIICIEKKSLPS
SISEQNLIEKIQTHLLPRKLPIQLQLMSSLPRNQHGKLDRKALVLNIDGLENKEKNNAK

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=380, Percent_Identity=26.3157894736842, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI187761343, Length=380, Percent_Identity=26.3157894736842, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI156151445, Length=353, Percent_Identity=26.628895184136, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI145693145, Length=344, Percent_Identity=25, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1788107, Length=347, Percent_Identity=27.0893371757925, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1786801, Length=541, Percent_Identity=25.1386321626617, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1786810, Length=396, Percent_Identity=20.7070707070707, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1788595, Length=358, Percent_Identity=26.2569832402235, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17558820, Length=403, Percent_Identity=25.0620347394541, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17531443, Length=366, Percent_Identity=26.5027322404372, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI32563687, Length=486, Percent_Identity=22.0164609053498, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17557194, Length=362, Percent_Identity=26.2430939226519, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17538037, Length=360, Percent_Identity=25.5555555555556, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17559526, Length=366, Percent_Identity=25.9562841530055, Blast_Score=79, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17560308, Length=301, Percent_Identity=24.9169435215947, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17556356, Length=242, Percent_Identity=26.8595041322314, Blast_Score=65, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=344, Percent_Identity=25.5813953488372, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI18859661, Length=458, Percent_Identity=26.2008733624454, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24581924, Length=357, Percent_Identity=28.0112044817927, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21355181, Length=375, Percent_Identity=26.9333333333333, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24648676, Length=379, Percent_Identity=23.4828496042216, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24653035, Length=362, Percent_Identity=22.6519337016575, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 50872; Mature: 50872

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFLASDVPNKVAITHGNRVITFDELTHEVLASSKQFLKLPKTIIVLHATPEIGFIIQLL
CEEEECCCCCEEEEECCCEEEEHHHHHHHHHHCCHHHHCCCCEEEEEECCCCHHHHHHHH
ACLETGRPIALFPNSISEEEKQRRLSLLGNAVMIDEEGELQEIFENKTIEPHPQTALILF
HHHHCCCCEEECCCCCCHHHHHHHHHHHCCEEEECCCCHHHHHHCCCCCCCCCCEEEEEE
TSGSTGKVKAVQLSSINIKANCHAVIKALEFKKIQDQLLFLPLSYSFGLLGQLLPGLMFG
ECCCCCEEEEEEEEEEEEECHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHH
LTTRLITHFTDIKMLMEQSTVPQMWSGVPSHWVAINAIAKRYPDSAAKINAIVSAGAPLS
HHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHEECCCCHH
TALRTDLKQIFPNAIIYNNYGLTEASPRVLTYSSKDPLFTENYAGYPVGDWKVKLSAEHE
HHHHHHHHHHCCCEEEEECCCCCCCCCCEEEECCCCCCEECCCCCCCCCCEEEEEECCCE
LLISGTQMMLGYLGEKDSTKIQNGWLSTGDIAEILPNGLVAIKGRRDSLVNIGGEKVNLS
EEEECHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCEEEEECCCCCEEECCCCEEEHH
EIEEKICQIDVIKEAIVIPQEDKIYGTRLIICIEKKSLPSSISEQNLIEKIQTHLLPRKL
HHHHHHHHHHHHHHHHCCCCCCCEECEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCC
PIQLQLMSSLPRNQHGKLDRKALVLNIDGLENKEKNNAK
CEEEEHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MLFLASDVPNKVAITHGNRVITFDELTHEVLASSKQFLKLPKTIIVLHATPEIGFIIQLL
CEEEECCCCCEEEEECCCEEEEHHHHHHHHHHCCHHHHCCCCEEEEEECCCCHHHHHHHH
ACLETGRPIALFPNSISEEEKQRRLSLLGNAVMIDEEGELQEIFENKTIEPHPQTALILF
HHHHCCCCEEECCCCCCHHHHHHHHHHHCCEEEECCCCHHHHHHCCCCCCCCCCEEEEEE
TSGSTGKVKAVQLSSINIKANCHAVIKALEFKKIQDQLLFLPLSYSFGLLGQLLPGLMFG
ECCCCCEEEEEEEEEEEEECHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHH
LTTRLITHFTDIKMLMEQSTVPQMWSGVPSHWVAINAIAKRYPDSAAKINAIVSAGAPLS
HHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHEECCCCHH
TALRTDLKQIFPNAIIYNNYGLTEASPRVLTYSSKDPLFTENYAGYPVGDWKVKLSAEHE
HHHHHHHHHHCCCEEEEECCCCCCCCCCEEEECCCCCCEECCCCCCCCCCEEEEEECCCE
LLISGTQMMLGYLGEKDSTKIQNGWLSTGDIAEILPNGLVAIKGRRDSLVNIGGEKVNLS
EEEECHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCEEEEECCCCCEEECCCCEEEHH
EIEEKICQIDVIKEAIVIPQEDKIYGTRLIICIEKKSLPSSISEQNLIEKIQTHLLPRKL
HHHHHHHHHHHHHHHHCCCCCCCEECEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCC
PIQLQLMSSLPRNQHGKLDRKALVLNIDGLENKEKNNAK
CEEEEHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]