Click here to switch to the map view.

The map label for this gene is fadD25 [H]

Identifier: 54295074

GI number: 54295074

Start: 2461958

End: 2463703

Strand: Direct

Name: fadD25 [H]

Synonym: lpl2154

Alternate gene names: 54295074

Gene position: 2461958-2463703 (Clockwise)

Preceding gene: 54295073

Following gene: 54295075

Centisome position: 73.59

GC content: 40.89

Gene sequence:

>1746_bases
GTGAAAAAAGAATATTTGCAGTGCCAGTCCCTGGTTGATGTCGTAAGGTTACGTGCTTTACACAGCCCTAAGAAGAAAAG
CTGTACTTTTCTGAACAAAGAGTTGGAAGAGATAATGACTTATGAGCAACTGGATCAACACGCCAAAGCTATTGCGGCAA
CTTTGCAAGCGGAAGGAGCAAAACCTGGTGACAGGGTATTGCTGTTATTTGCGCCTGGATTGCCCCTTATCCAGGCCTTT
TTGGGCTGTCTTTACGCAGGTTGCATTGCTGTACCCATTTACCCACCAGCCCAAGAAAAATTATTGGACAAGGCACAACG
CATAGTTACCAACTCAAAACCGGTCATAGTACTGATGATTGCGGATCACATCAAAAAATTCACCGCAGACGAATTAAATA
CAAATCCCAAATTCCTGAAAATTCCTGCTATTGCGCTCGAGAGCATTGAGTTAAACAGAAGTAGTAGTTGGCAACCAACC
TCCATTAAGAGCAATGACATTGCGTTTTTACAATACACTTCCGGCTCAACCATGCACCCTAAAGGAGTGATGGTAAGCCA
CCATAATTTACTGGATAATCTGAATAAAATTTTTACCTCTTTTCATATGAATGATGAAACCATTATTTTCAGCTGGCTAC
CCCCACATCATGATATGGGTTTGATTGGCTGCATTCTGACACCCATCTATGGTGGAATTCAGGCAATCATGATGTCCCCT
TTCTCATTTTTACAAAACCCGCTTTCCTGGTTAAAACATATTACTAAATACAGAGCCACTATTAGTGGCAGCCCCAACTT
CGCTTACGATTATTGTGTCAAACGAATCAGGGAAGAAAAAAAAGAAGGTCTGGATTTAAGTTCATGGGTGACCGCTTTCA
ACGGTGCTGAGCCAGTACGAGCAGAAACCATGGAACATTTTTATCAGGCATTTAAAGAGTTTGGATTTCGCAAAGAAGCC
TTCTATCCATGCTATGGCCTGGCTGAGGCTACTTTGTTAGTGACGGGAGGAACACCAGGAAGTCCATACAAAACATTAAC
TCTGGCCAAAGAACAATTTCAAGATCATCGCGTACATTTTGCTGACGATAACAGTCCAGGCAGTTACAAGTTAGTCAGCA
GTGGTAATCCCATTCAAGCAGTTAAAATTATAGATCCTGATACCTTGATCCCATGTGATTTTGACCAGGTTGGTGAAATT
TGGGTACAAAGTAACAGTGTCGCCAAAGGATATTGGAATCAACCCGAAGAAACAGAGCATGCATTCTCAGGGAAAATTAA
AGAAAATGAGAATAGCCCAATCTATTTAAGAACCGGGGACTTGGGCTTTCTCCATGAAAATGAGTTATACGTTACTGGAC
GCATTAAAGACTTAATTATTATTTATGGTAAAAATCATTATCCTCAGGACATAGAGTTCAGCCTGATGCACTCTCCTCTC
CATCACGTATTGGGCAAATGCGCTGCTTTTGTGATTCAGGAAGAGCATGAATATAAACTGACTGTGATGTGTGAAGTAAA
AAATCGATTCATGGATGACGTAGCTCAAGACAATTTATTCAATGAGATTTTTGAGCTTGTTTACGAAAACCACCAATTGG
AGGTACATACTATAGTCCTGATTCCTCTTAAAGCAATGCCACATACCACCAGCGGAAAAATTCGCAGGAATTTTTGCCGA
AAACATCTTTTGGATAAAACTCTGCCAATAGTGGCTACCTGGCAACTCAATAAAATTGAGGAATAA

Upstream 100 bases:

>100_bases
CAGGAAAAATTCTCCTGATGAGTGTTGGTGGTGGTCTGTCCTATGCAGGACAAGTACTAAACTATCAAAAAGCCTCTTTA
AGCAATTAAGGTGTTTTATA

Downstream 100 bases:

>100_bases
AATCATGCTGTTTTTGGCATCAGATGTTCCAAACAAAGTAGCAATTACCCATGGCAATCGAGTAATAACTTTTGATGAAT
TAACACATGAAGTTTTAGCA

Product: hypothetical protein

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 581; Mature: 581

Protein sequence:

>581_residues
MKKEYLQCQSLVDVVRLRALHSPKKKSCTFLNKELEEIMTYEQLDQHAKAIAATLQAEGAKPGDRVLLLFAPGLPLIQAF
LGCLYAGCIAVPIYPPAQEKLLDKAQRIVTNSKPVIVLMIADHIKKFTADELNTNPKFLKIPAIALESIELNRSSSWQPT
SIKSNDIAFLQYTSGSTMHPKGVMVSHHNLLDNLNKIFTSFHMNDETIIFSWLPPHHDMGLIGCILTPIYGGIQAIMMSP
FSFLQNPLSWLKHITKYRATISGSPNFAYDYCVKRIREEKKEGLDLSSWVTAFNGAEPVRAETMEHFYQAFKEFGFRKEA
FYPCYGLAEATLLVTGGTPGSPYKTLTLAKEQFQDHRVHFADDNSPGSYKLVSSGNPIQAVKIIDPDTLIPCDFDQVGEI
WVQSNSVAKGYWNQPEETEHAFSGKIKENENSPIYLRTGDLGFLHENELYVTGRIKDLIIIYGKNHYPQDIEFSLMHSPL
HHVLGKCAAFVIQEEHEYKLTVMCEVKNRFMDDVAQDNLFNEIFELVYENHQLEVHTIVLIPLKAMPHTTSGKIRRNFCR
KHLLDKTLPIVATWQLNKIEE

Sequences:

>Translated_581_residues
MKKEYLQCQSLVDVVRLRALHSPKKKSCTFLNKELEEIMTYEQLDQHAKAIAATLQAEGAKPGDRVLLLFAPGLPLIQAF
LGCLYAGCIAVPIYPPAQEKLLDKAQRIVTNSKPVIVLMIADHIKKFTADELNTNPKFLKIPAIALESIELNRSSSWQPT
SIKSNDIAFLQYTSGSTMHPKGVMVSHHNLLDNLNKIFTSFHMNDETIIFSWLPPHHDMGLIGCILTPIYGGIQAIMMSP
FSFLQNPLSWLKHITKYRATISGSPNFAYDYCVKRIREEKKEGLDLSSWVTAFNGAEPVRAETMEHFYQAFKEFGFRKEA
FYPCYGLAEATLLVTGGTPGSPYKTLTLAKEQFQDHRVHFADDNSPGSYKLVSSGNPIQAVKIIDPDTLIPCDFDQVGEI
WVQSNSVAKGYWNQPEETEHAFSGKIKENENSPIYLRTGDLGFLHENELYVTGRIKDLIIIYGKNHYPQDIEFSLMHSPL
HHVLGKCAAFVIQEEHEYKLTVMCEVKNRFMDDVAQDNLFNEIFELVYENHQLEVHTIVLIPLKAMPHTTSGKIRRNFCR
KHLLDKTLPIVATWQLNKIEE
>Mature_581_residues
MKKEYLQCQSLVDVVRLRALHSPKKKSCTFLNKELEEIMTYEQLDQHAKAIAATLQAEGAKPGDRVLLLFAPGLPLIQAF
LGCLYAGCIAVPIYPPAQEKLLDKAQRIVTNSKPVIVLMIADHIKKFTADELNTNPKFLKIPAIALESIELNRSSSWQPT
SIKSNDIAFLQYTSGSTMHPKGVMVSHHNLLDNLNKIFTSFHMNDETIIFSWLPPHHDMGLIGCILTPIYGGIQAIMMSP
FSFLQNPLSWLKHITKYRATISGSPNFAYDYCVKRIREEKKEGLDLSSWVTAFNGAEPVRAETMEHFYQAFKEFGFRKEA
FYPCYGLAEATLLVTGGTPGSPYKTLTLAKEQFQDHRVHFADDNSPGSYKLVSSGNPIQAVKIIDPDTLIPCDFDQVGEI
WVQSNSVAKGYWNQPEETEHAFSGKIKENENSPIYLRTGDLGFLHENELYVTGRIKDLIIIYGKNHYPQDIEFSLMHSPL
HHVLGKCAAFVIQEEHEYKLTVMCEVKNRFMDDVAQDNLFNEIFELVYENHQLEVHTIVLIPLKAMPHTTSGKIRRNFCR
KHLLDKTLPIVATWQLNKIEE

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI225735629, Length=602, Percent_Identity=27.4086378737542, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI45827692, Length=602, Percent_Identity=27.4086378737542, Blast_Score=173, Evalue=4e-43,
Organism=Homo sapiens, GI44888818, Length=596, Percent_Identity=26.3422818791946, Blast_Score=168, Evalue=1e-41,
Organism=Homo sapiens, GI55749758, Length=514, Percent_Identity=27.431906614786, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI225735625, Length=596, Percent_Identity=22.9865771812081, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI45827694, Length=546, Percent_Identity=23.0769230769231, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI225735627, Length=494, Percent_Identity=23.4817813765182, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI45827696, Length=494, Percent_Identity=23.4817813765182, Blast_Score=100, Evalue=6e-21,
Organism=Homo sapiens, GI45827698, Length=466, Percent_Identity=23.8197424892704, Blast_Score=89, Evalue=1e-17,
Organism=Escherichia coli, GI1788107, Length=519, Percent_Identity=26.3969171483622, Blast_Score=119, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI193202679, Length=537, Percent_Identity=23.463687150838, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17531443, Length=580, Percent_Identity=24.1379310344828, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI71994690, Length=372, Percent_Identity=24.4623655913978, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71994694, Length=372, Percent_Identity=24.4623655913978, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71994703, Length=372, Percent_Identity=24.4623655913978, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24654656, Length=466, Percent_Identity=27.6824034334764, Blast_Score=157, Evalue=3e-38,
Organism=Drosophila melanogaster, GI18859661, Length=557, Percent_Identity=23.6983842010772, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 65973; Mature: 65973

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKEYLQCQSLVDVVRLRALHSPKKKSCTFLNKELEEIMTYEQLDQHAKAIAATLQAEGA
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KPGDRVLLLFAPGLPLIQAFLGCLYAGCIAVPIYPPAQEKLLDKAQRIVTNSKPVIVLMI
CCCCEEEEEEECCHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEE
ADHIKKFTADELNTNPKFLKIPAIALESIELNRSSSWQPTSIKSNDIAFLQYTSGSTMHP
HHHHHHHHHHHCCCCCCEEEECCEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCC
KGVMVSHHNLLDNLNKIFTSFHMNDETIIFSWLPPHHDMGLIGCILTPIYGGIQAIMMSP
CCEEEEHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCH
FSFLQNPLSWLKHITKYRATISGSPNFAYDYCVKRIREEKKEGLDLSSWVTAFNGAEPVR
HHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHH
AETMEHFYQAFKEFGFRKEAFYPCYGLAEATLLVTGGTPGSPYKTLTLAKEQFQDHRVHF
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEHHHHHHHHHCCCEEE
ADDNSPGSYKLVSSGNPIQAVKIIDPDTLIPCDFDQVGEIWVQSNSVAKGYWNQPEETEH
ECCCCCCCEEEEECCCCEEEEEEECCCCCCCCCHHHHCCEEEECCCEECCCCCCCCHHHH
AFSGKIKENENSPIYLRTGDLGFLHENELYVTGRIKDLIIIYGKNHYPQDIEFSLMHSPL
HHCCCCCCCCCCEEEEEECCCCEEECCCEEEEEEEEEEEEEECCCCCCCCCCHHHHHHHH
HHVLGKCAAFVIQEEHEYKLTVMCEVKNRFMDDVAQDNLFNEIFELVYENHQLEVHTIVL
HHHHHHHHHHHEECCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEE
IPLKAMPHTTSGKIRRNFCRKHLLDKTLPIVATWQLNKIEE
EEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCC
>Mature Secondary Structure
MKKEYLQCQSLVDVVRLRALHSPKKKSCTFLNKELEEIMTYEQLDQHAKAIAATLQAEGA
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KPGDRVLLLFAPGLPLIQAFLGCLYAGCIAVPIYPPAQEKLLDKAQRIVTNSKPVIVLMI
CCCCEEEEEEECCHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEE
ADHIKKFTADELNTNPKFLKIPAIALESIELNRSSSWQPTSIKSNDIAFLQYTSGSTMHP
HHHHHHHHHHHCCCCCCEEEECCEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCC
KGVMVSHHNLLDNLNKIFTSFHMNDETIIFSWLPPHHDMGLIGCILTPIYGGIQAIMMSP
CCEEEEHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCH
FSFLQNPLSWLKHITKYRATISGSPNFAYDYCVKRIREEKKEGLDLSSWVTAFNGAEPVR
HHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHH
AETMEHFYQAFKEFGFRKEAFYPCYGLAEATLLVTGGTPGSPYKTLTLAKEQFQDHRVHF
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEHHHHHHHHHCCCEEE
ADDNSPGSYKLVSSGNPIQAVKIIDPDTLIPCDFDQVGEIWVQSNSVAKGYWNQPEETEH
ECCCCCCCEEEEECCCCEEEEEEECCCCCCCCCHHHHCCEEEECCCEECCCCCCCCHHHH
AFSGKIKENENSPIYLRTGDLGFLHENELYVTGRIKDLIIIYGKNHYPQDIEFSLMHSPL
HHCCCCCCCCCCEEEEEECCCCEEECCCEEEEEEEEEEEEEECCCCCCCCCCHHHHHHHH
HHVLGKCAAFVIQEEHEYKLTVMCEVKNRFMDDVAQDNLFNEIFELVYENHQLEVHTIVL
HHHHHHHHHHHEECCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEE
IPLKAMPHTTSGKIRRNFCRKHLLDKTLPIVATWQLNKIEE
EEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]