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The map label for this gene is rlmN [H]

Identifier: 54294410

GI number: 54294410

Start: 1647541

End: 1648689

Strand: Direct

Name: rlmN [H]

Synonym: lpl1479

Alternate gene names: 54294410

Gene position: 1647541-1648689 (Clockwise)

Preceding gene: 54294409

Following gene: 54294411

Centisome position: 49.24

GC content: 36.55

Gene sequence:

>1149_bases
ATGGACCAACAAAAAGTGAATCTATTAAATTATAATTACTTACAGCTACGAGAATTATTGATGGCGTGGGATGAAAAGCC
TTTCAGAGCTCAACAGCTATTTCAATGGATTCATCAAGTTGGCATACGTGACTTTGCCCAGATGACTAATTTAGGGAAAG
TTTTAAGAAATAAACTTTCCCAATTAGCTTGTATAGATTTACCTGAAATCGTTGCATGTCAAAAATCAGCTGATGGCACA
CATAAGTGGCTATTAAAGCTGGAATGTGGGAATTGTATTGAAACAGTGTTCATTCCAGAAGCAAATCGAGGGACACTTTG
TGTTTCATCACAAGTAGGTTGTGCTTTAAATTGTTCTTTTTGTTCCACAGCAAAGCAAGGATTTAACAGAAATTTATCAA
CAGCTGAAATAATTGGTCAAGTATGGCTGGCAGCACGCGAACTATCAGATAATGATGGCACTCACGATAAAAAAATAACT
AACGTCGTGATGATGGGAATGGGTGAACCGTTACTTAATTTCGATAATGTTGTTTCTGCTATGAACATCATGATGGATGA
TTTAGCTTATGGACTATCTAAAAGACGGGTTACATTAAGTACTTCGGGTGTTATACCGGAAATGGAGCGACTCAGAGAAG
TCAGTCCTGTCGCGTTGGCAGTATCCTTACATGCACCAACAGATGAACTACGTAACGAGCTGGTACCAATTAATAAAAAA
TATCCTCTCTCTCAATTAATATCACTATGTAAACGATATTTTAAAGACGAACCAAGAAGAAAAGTTACTTTTGAATACGT
CATGCTAAAAGGGGTTAATGATCAACCTGAACATGCTAGTCAATTAATTAAACTTTTGCATAATGTGCCTGCAAAAGTTA
ATTTAATTCCTTTTAACCCATTTCCTTTAACTCAATATCAGAGATCATCCCGGGAAACAATTGATGCCTTTCGAGATAAA
TTAATGAAACATGGTATTAATACTATTACTCGCAAAACTCGTGGAGATGATATTGATGCAGCATGCGGCCAACTTGCTGG
CGAGGTAAAAGATAAAACGAGCAGATCACAACGATGGCAAAAACTTCATTTCATGAGCAAAACAGACAAATCAACTGAAT
TGACCATTTCTTCGGAAGAAATTGCATAA

Upstream 100 bases:

>100_bases
TGCATGGTTCTGACAGCCTTGAAAATGCTGCTCGTGAAATAGCATTTTTCTTTGAACCGCATGAGTTGTGCAATAGATAA
AGGCAGGCTTGGGAGCGGTA

Downstream 100 bases:

>100_bases
CACAGTCTTGTATAATAACCGAGAGCCAATTAAGAACTTTCTCCAGTATTCCTGGTTGGTGATTATGTATAATTAAGGCG
TTTTCACCTGCAGACTCACA

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MDQQKVNLLNYNYLQLRELLMAWDEKPFRAQQLFQWIHQVGIRDFAQMTNLGKVLRNKLSQLACIDLPEIVACQKSADGT
HKWLLKLECGNCIETVFIPEANRGTLCVSSQVGCALNCSFCSTAKQGFNRNLSTAEIIGQVWLAARELSDNDGTHDKKIT
NVVMMGMGEPLLNFDNVVSAMNIMMDDLAYGLSKRRVTLSTSGVIPEMERLREVSPVALAVSLHAPTDELRNELVPINKK
YPLSQLISLCKRYFKDEPRRKVTFEYVMLKGVNDQPEHASQLIKLLHNVPAKVNLIPFNPFPLTQYQRSSRETIDAFRDK
LMKHGINTITRKTRGDDIDAACGQLAGEVKDKTSRSQRWQKLHFMSKTDKSTELTISSEEIA

Sequences:

>Translated_382_residues
MDQQKVNLLNYNYLQLRELLMAWDEKPFRAQQLFQWIHQVGIRDFAQMTNLGKVLRNKLSQLACIDLPEIVACQKSADGT
HKWLLKLECGNCIETVFIPEANRGTLCVSSQVGCALNCSFCSTAKQGFNRNLSTAEIIGQVWLAARELSDNDGTHDKKIT
NVVMMGMGEPLLNFDNVVSAMNIMMDDLAYGLSKRRVTLSTSGVIPEMERLREVSPVALAVSLHAPTDELRNELVPINKK
YPLSQLISLCKRYFKDEPRRKVTFEYVMLKGVNDQPEHASQLIKLLHNVPAKVNLIPFNPFPLTQYQRSSRETIDAFRDK
LMKHGINTITRKTRGDDIDAACGQLAGEVKDKTSRSQRWQKLHFMSKTDKSTELTISSEEIA
>Mature_382_residues
MDQQKVNLLNYNYLQLRELLMAWDEKPFRAQQLFQWIHQVGIRDFAQMTNLGKVLRNKLSQLACIDLPEIVACQKSADGT
HKWLLKLECGNCIETVFIPEANRGTLCVSSQVGCALNCSFCSTAKQGFNRNLSTAEIIGQVWLAARELSDNDGTHDKKIT
NVVMMGMGEPLLNFDNVVSAMNIMMDDLAYGLSKRRVTLSTSGVIPEMERLREVSPVALAVSLHAPTDELRNELVPINKK
YPLSQLISLCKRYFKDEPRRKVTFEYVMLKGVNDQPEHASQLIKLLHNVPAKVNLIPFNPFPLTQYQRSSRETIDAFRDK
LMKHGINTITRKTRGDDIDAACGQLAGEVKDKTSRSQRWQKLHFMSKTDKSTELTISSEEIA

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=356, Percent_Identity=56.7415730337079, Blast_Score=412, Evalue=1e-116,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 43306; Mature: 43306

Theoretical pI: Translated: 8.55; Mature: 8.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQQKVNLLNYNYLQLRELLMAWDEKPFRAQQLFQWIHQVGIRDFAQMTNLGKVLRNKLS
CCCCCCCHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLACIDLPEIVACQKSADGTHKWLLKLECGNCIETVFIPEANRGTLCVSSQVGCALNCSF
HHHHCCCHHHHHHCCCCCCCEEEEEEEECCCEEEEEEECCCCCCCEEEECCCCCEEECHH
CSTAKQGFNRNLSTAEIIGQVWLAARELSDNDGTHDKKITNVVMMGMGEPLLNFDNVVSA
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHH
MNIMMDDLAYGLSKRRVTLSTSGVIPEMERLREVSPVALAVSLHAPTDELRNELVPINKK
HHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHCCCCEEEEEEECCCHHHHHHCCCCCCCC
YPLSQLISLCKRYFKDEPRRKVTFEYVMLKGVNDQPEHASQLIKLLHNVPAKVNLIPFNP
CCHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCC
FPLTQYQRSSRETIDAFRDKLMKHGINTITRKTRGDDIDAACGQLAGEVKDKTSRSQRWQ
CCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KLHFMSKTDKSTELTISSEEIA
HHHHHHCCCCCCEEEECHHCCC
>Mature Secondary Structure
MDQQKVNLLNYNYLQLRELLMAWDEKPFRAQQLFQWIHQVGIRDFAQMTNLGKVLRNKLS
CCCCCCCHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLACIDLPEIVACQKSADGTHKWLLKLECGNCIETVFIPEANRGTLCVSSQVGCALNCSF
HHHHCCCHHHHHHCCCCCCCEEEEEEEECCCEEEEEEECCCCCCCEEEECCCCCEEECHH
CSTAKQGFNRNLSTAEIIGQVWLAARELSDNDGTHDKKITNVVMMGMGEPLLNFDNVVSA
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHH
MNIMMDDLAYGLSKRRVTLSTSGVIPEMERLREVSPVALAVSLHAPTDELRNELVPINKK
HHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHCCCCEEEEEEECCCHHHHHHCCCCCCCC
YPLSQLISLCKRYFKDEPRRKVTFEYVMLKGVNDQPEHASQLIKLLHNVPAKVNLIPFNP
CCHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCC
FPLTQYQRSSRETIDAFRDKLMKHGINTITRKTRGDDIDAACGQLAGEVKDKTSRSQRWQ
CCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KLHFMSKTDKSTELTISSEEIA
HHHHHHCCCCCCEEEECHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA