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The map label for this gene is miaE [H]

Identifier: 54294407

GI number: 54294407

Start: 1645379

End: 1646002

Strand: Reverse

Name: miaE [H]

Synonym: lpl1476

Alternate gene names: 54294407

Gene position: 1646002-1645379 (Counterclockwise)

Preceding gene: 54294417

Following gene: 54294403

Centisome position: 49.2

GC content: 36.06

Gene sequence:

>624_bases
ATGTTAAAACGATTAGATAGTGATTTACAAACGTTAAATGATTTTTTAAAAGTTAAAACGCCGGAAACGTGGTTACATTA
TGCTGCTGCTAATATACCTCTTTTATTGCTCGATCATGCTCATTGTGAACGTAAGGCAGCTGGAACAGCAATTAATTTCA
TAAGCAAGTATCCAGAAAAAGCAGAGTTAATAGCAATTATGGCACCATTGGCAAGAGAAGAGTTGCTACACTTTGAAAAA
GTCATTGATATCATGAAGCAAAAAGGAATTTTTTATGCTCCTTTGCAGCCATCAGACTATGCATCCAATTTGCATAAACA
TGTCACAAATAAAGATGGAATTGAGCGGTTGTGTGATCAGTTGATTATAGGCGCTATCATTGAGGCTAGATCTTGCGAGC
GTTTTAATTCGATAATTCCGTATATAGAAGATCAGGACTTAGCCAAATTTTATAAAACATTGGTTAAATCAGAATGCAGA
CATTTTGAAGAATATTTGCATTTGGCCAAGCTCTATGGCGGTTCTGTTGATGAGCGTATTTCGGAGTTTTTAACTATAGA
AAATTCGTTTATCTTATCAGACGATGTGGTATTTCGCTTTCATAGTGGCTTGCCTCCGGCTTGA

Upstream 100 bases:

>100_bases
TTCATCATCTTTTTTGATCTCGTCCATTTTTTATCCAATTTAAATTCTTTAAAGTATGTGATATAAGGGCTATTTATTTG
AAATTATAGTGTATTTTATC

Downstream 100 bases:

>100_bases
TGCCTTAAAACAGAGTCAACAGATTTTTCAGCACTAGCCCATAAGTATTATATACAGGCTAGTGTAACCCAATATTTCTC
TTGCTTAATCAAGGTCTGCT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MLKRLDSDLQTLNDFLKVKTPETWLHYAAANIPLLLLDHAHCERKAAGTAINFISKYPEKAELIAIMAPLAREELLHFEK
VIDIMKQKGIFYAPLQPSDYASNLHKHVTNKDGIERLCDQLIIGAIIEARSCERFNSIIPYIEDQDLAKFYKTLVKSECR
HFEEYLHLAKLYGGSVDERISEFLTIENSFILSDDVVFRFHSGLPPA

Sequences:

>Translated_207_residues
MLKRLDSDLQTLNDFLKVKTPETWLHYAAANIPLLLLDHAHCERKAAGTAINFISKYPEKAELIAIMAPLAREELLHFEK
VIDIMKQKGIFYAPLQPSDYASNLHKHVTNKDGIERLCDQLIIGAIIEARSCERFNSIIPYIEDQDLAKFYKTLVKSECR
HFEEYLHLAKLYGGSVDERISEFLTIENSFILSDDVVFRFHSGLPPA
>Mature_207_residues
MLKRLDSDLQTLNDFLKVKTPETWLHYAAANIPLLLLDHAHCERKAAGTAINFISKYPEKAELIAIMAPLAREELLHFEK
VIDIMKQKGIFYAPLQPSDYASNLHKHVTNKDGIERLCDQLIIGAIIEARSCERFNSIIPYIEDQDLAKFYKTLVKSECR
HFEEYLHLAKLYGGSVDERISEFLTIENSFILSDDVVFRFHSGLPPA

Specific function: Production of the modified nucleoside 2-methylthio-cis- ribozeatin (MS[2]IO[6]A) found in some tRNAs. Catalyzes the oxygen-dependent transformation of MS[2]I[6]A into MS[2]IO[6]A [H]

COG id: COG4445

COG function: function code FJ; Hydroxylase for synthesis of 2-methylthio-cis-ribozeatin in tRNA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012347
- InterPro:   IPR010386 [H]

Pfam domain/function: PF06175 MiaE [H]

EC number: NA

Molecular weight: Translated: 23720; Mature: 23720

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRLDSDLQTLNDFLKVKTPETWLHYAAANIPLLLLDHAHCERKAAGTAINFISKYPEK
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHCCCH
AELIAIMAPLAREELLHFEKVIDIMKQKGIFYAPLQPSDYASNLHKHVTNKDGIERLCDQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHCCCHHHHHHHHHH
LIIGAIIEARSCERFNSIIPYIEDQDLAKFYKTLVKSECRHFEEYLHLAKLYGGSVDERI
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
SEFLTIENSFILSDDVVFRFHSGLPPA
HHHHHHCCCEEECHHHHHHCCCCCCCC
>Mature Secondary Structure
MLKRLDSDLQTLNDFLKVKTPETWLHYAAANIPLLLLDHAHCERKAAGTAINFISKYPEK
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHCCCH
AELIAIMAPLAREELLHFEKVIDIMKQKGIFYAPLQPSDYASNLHKHVTNKDGIERLCDQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHCCCHHHHHHHHHH
LIIGAIIEARSCERFNSIIPYIEDQDLAKFYKTLVKSECRHFEEYLHLAKLYGGSVDERI
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
SEFLTIENSFILSDDVVFRFHSGLPPA
HHHHHHCCCEEECHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253666; 11677609 [H]