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The map label for this gene is lpxH [H]

Identifier: 54294405

GI number: 54294405

Start: 1643819

End: 1644547

Strand: Direct

Name: lpxH [H]

Synonym: lpl1474

Alternate gene names: 54294405

Gene position: 1643819-1644547 (Clockwise)

Preceding gene: 54294404

Following gene: 54294406

Centisome position: 49.13

GC content: 34.84

Gene sequence:

>729_bases
ATGATTGACTCCGTTTTTATATCCGATTTACATCTGCATCCAGAAGATAATGAAATTCAAAGCAGATTTGATCATTTTGT
AAATTGGGCTCGGCTATCTGTAAAAAGGATATACATACTAGGTGATTTTTTTCATGCCTGGTCAGGTGATGATTCAATTA
ACGCATGGAGTGCTGCTATTGCAGAACAAATAGAATCATTGACTCAAAGCGGCATTGAAATTTATTTTATGACTGGAAAT
CGTGATTTTTTACTCGGCTCATTATTTGCCAAGCGAACAGGTTGGAAAATATTAACAGAGCCAACCATCATTACACTAGG
AAATCACAAGATCTTGCTGGTTCATGGCGATCGTTATTGTACTAATGACAGATCACACCAACGATTCCGATTATTAACGA
GAAACAGGTGGTTCTCATTTCTTTTTTTAAGACTTCCTTTATCATTTCGCAAACGATTAGTTCACGGAGTTCGCAAGATT
AGTCTAAAAAATCACCATAAATCAATCGAACAGATGGATATTGTTACAGAGGCTGCAATTCGACATATGTCTGAAAATAA
TACAGAAATACTGATTCATGGACACACGCATAAACCTGGCATAACAAATTACACTTATGAAGAAAAAATACTGACAAGAT
ATGTTCTAAGTGATTGGGATGACATACCCAAACTATTGTGTTATGATAATACAAAAGGATTCTATTTCACCCAGTATGAT
ATAAATTGA

Upstream 100 bases:

>100_bases
TATCTATAGCGGGTTTCTATCGAATAAGTGATGCTATTGAACCAGTTAATGGGCCTTGTCAGATCTACTTGGCAAATGAA
CATATCGTTGTTGAACCATT

Downstream 100 bases:

>100_bases
GGAGTTACCCAATGTCTAAACCATCAAATAAAATGAAAGAGGAACTCGAACAAATTCGAAAAACTGAAATAGAAAATGCT
ATAGCAAAAGAACGAGAAGA

Product: UDP-2,3-diacylglucosamine hydrolase

Products: 2,3-bis(3-hydroxymyristoyl)-beta-D-glucosaminyl 1; UMP [C]

Alternate protein names: NA

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MIDSVFISDLHLHPEDNEIQSRFDHFVNWARLSVKRIYILGDFFHAWSGDDSINAWSAAIAEQIESLTQSGIEIYFMTGN
RDFLLGSLFAKRTGWKILTEPTIITLGNHKILLVHGDRYCTNDRSHQRFRLLTRNRWFSFLFLRLPLSFRKRLVHGVRKI
SLKNHHKSIEQMDIVTEAAIRHMSENNTEILIHGHTHKPGITNYTYEEKILTRYVLSDWDDIPKLLCYDNTKGFYFTQYD
IN

Sequences:

>Translated_242_residues
MIDSVFISDLHLHPEDNEIQSRFDHFVNWARLSVKRIYILGDFFHAWSGDDSINAWSAAIAEQIESLTQSGIEIYFMTGN
RDFLLGSLFAKRTGWKILTEPTIITLGNHKILLVHGDRYCTNDRSHQRFRLLTRNRWFSFLFLRLPLSFRKRLVHGVRKI
SLKNHHKSIEQMDIVTEAAIRHMSENNTEILIHGHTHKPGITNYTYEEKILTRYVLSDWDDIPKLLCYDNTKGFYFTQYD
IN
>Mature_242_residues
MIDSVFISDLHLHPEDNEIQSRFDHFVNWARLSVKRIYILGDFFHAWSGDDSINAWSAAIAEQIESLTQSGIEIYFMTGN
RDFLLGSLFAKRTGWKILTEPTIITLGNHKILLVHGDRYCTNDRSHQRFRLLTRNRWFSFLFLRLPLSFRKRLVHGVRKI
SLKNHHKSIEQMDIVTEAAIRHMSENNTEILIHGHTHKPGITNYTYEEKILTRYVLSDWDDIPKLLCYDNTKGFYFTQYD
IN

Specific function: Catalyzes the hydrolysis of the pyrophosphate bond of UDP-2,3-diacylglucosamine to yield 2,3-diacylglucosamine 1- phosphate (lipid X) and UMP [H]

COG id: COG2908

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lpxH family [H]

Homologues:

Organism=Escherichia coli, GI1786735, Length=219, Percent_Identity=37.8995433789954, Blast_Score=164, Evalue=5e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR010138 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 28545; Mature: 28545

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDSVFISDLHLHPEDNEIQSRFDHFVNWARLSVKRIYILGDFFHAWSGDDSINAWSAAI
CCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHEEEEEEEEEEHHHHCCCCCCCHHHHHHH
AEQIESLTQSGIEIYFMTGNRDFLLGSLFAKRTGWKILTEPTIITLGNHKILLVHGDRYC
HHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCCEEEECCEEEEECCEEEEEEECCEEC
TNDRSHQRFRLLTRNRWFSFLFLRLPLSFRKRLVHGVRKISLKNHHKSIEQMDIVTEAAI
CCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHMSENNTEILIHGHTHKPGITNYTYEEKILTRYVLSDWDDIPKLLCYDNTKGFYFTQYD
HHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEEE
IN
CC
>Mature Secondary Structure
MIDSVFISDLHLHPEDNEIQSRFDHFVNWARLSVKRIYILGDFFHAWSGDDSINAWSAAI
CCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHEEEEEEEEEEHHHHCCCCCCCHHHHHHH
AEQIESLTQSGIEIYFMTGNRDFLLGSLFAKRTGWKILTEPTIITLGNHKILLVHGDRYC
HHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCCEEEECCEEEEECCEEEEEEECCEEC
TNDRSHQRFRLLTRNRWFSFLFLRLPLSFRKRLVHGVRKISLKNHHKSIEQMDIVTEAAI
CCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHMSENNTEILIHGHTHKPGITNYTYEEKILTRYVLSDWDDIPKLLCYDNTKGFYFTQYD
HHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEEE
IN
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; UDP-2,3-bis(3-hydroxymyristoyl)glucosamine [C]

Specific reaction: H2O + UDP-2,3-bis(3-hydroxymyristoyl)glucosamine = 2,3-bis(3-hydroxymyristoyl)-beta-D-glucosaminyl 1 + UMP [C]

General reaction: Lipid A biosynthesis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA