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The map label for this gene is ruvC [H]

Identifier: 54294186

GI number: 54294186

Start: 1393372

End: 1393896

Strand: Direct

Name: ruvC [H]

Synonym: lpl1250

Alternate gene names: 54294186

Gene position: 1393372-1393896 (Clockwise)

Preceding gene: 54294185

Following gene: 54294187

Centisome position: 41.65

GC content: 41.52

Gene sequence:

>525_bases
ATGACGATTATATTAGGGATAGACCCAGGCTCACGGGTTACAGGATATGGATTGATTAAAGAATCTGACCGTAAAATCGC
GTACATCGATAGCGGTTGTATTCGCACATCAAACGATGTGGAATTAAGTCATAAATTATTGCAAATATATGATGGAATAT
GTGAGCTAATGGATCACTATTCCCCAACTGAGGTTGCTATTGAGCAGATATTTATGCATAACAATCCTAATTCAGCTTTG
AAATTAGGGCATGCGCGGGGGGTTGCCATGGTAGCAGCAGCCTCGCATCGTGTGAAAATCTGTGAGTATTCAGCAAGAGA
AATAAAACAAAGTGTGGTTGGTTATGGGGCCGCTGAAAAAGATCAAGTAAGTCATATGGTTGTAGAGCTTCTTCAGCTCA
ATCGAGCTCCTCAAAAAGACGCTGCCGATGCTTTGGCAATAGCAATATGCCATAGTCATATGAGGAATGGTTTGTCAAAA
TTAATCGGTTCAAGAGGAACGAAACGCAGGAGGATGAGACTATGA

Upstream 100 bases:

>100_bases
GTTTAATTAAACTGATTGATATGCTGGAAGATTTGGATGATGTCCAAGAAGTTTACAGCAATGCTGAATTTTCTGAAAAA
ATATTAGAATCAATGAATTA

Downstream 100 bases:

>100_bases
TAGGCTGGCTCCATGGTCAAATTATAGATAAGCATCAGCCAGGAAAATTGGTTCTTGATGTCAATGGCGTAGGATATGAT
GTAGAAACATCTTTGAATAC

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC [H]

Number of amino acids: Translated: 174; Mature: 173

Protein sequence:

>174_residues
MTIILGIDPGSRVTGYGLIKESDRKIAYIDSGCIRTSNDVELSHKLLQIYDGICELMDHYSPTEVAIEQIFMHNNPNSAL
KLGHARGVAMVAAASHRVKICEYSAREIKQSVVGYGAAEKDQVSHMVVELLQLNRAPQKDAADALAIAICHSHMRNGLSK
LIGSRGTKRRRMRL

Sequences:

>Translated_174_residues
MTIILGIDPGSRVTGYGLIKESDRKIAYIDSGCIRTSNDVELSHKLLQIYDGICELMDHYSPTEVAIEQIFMHNNPNSAL
KLGHARGVAMVAAASHRVKICEYSAREIKQSVVGYGAAEKDQVSHMVVELLQLNRAPQKDAADALAIAICHSHMRNGLSK
LIGSRGTKRRRMRL
>Mature_173_residues
TIILGIDPGSRVTGYGLIKESDRKIAYIDSGCIRTSNDVELSHKLLQIYDGICELMDHYSPTEVAIEQIFMHNNPNSALK
LGHARGVAMVAAASHRVKICEYSAREIKQSVVGYGAAEKDQVSHMVVELLQLNRAPQKDAADALAIAICHSHMRNGLSKL
IGSRGTKRRRMRL

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family [H]

Homologues:

Organism=Escherichia coli, GI1788170, Length=174, Percent_Identity=54.0229885057471, Blast_Score=164, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176 [H]

Pfam domain/function: PF02075 RuvC [H]

EC number: =3.1.22.4 [H]

Molecular weight: Translated: 19198; Mature: 19066

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIILGIDPGSRVTGYGLIKESDRKIAYIDSGCIRTSNDVELSHKLLQIYDGICELMDHY
CEEEEECCCCCCEEEEEEEECCCCEEEEEECCCEECCCCHHHHHHHHHHHHHHHHHHHCC
SPTEVAIEQIFMHNNPNSALKLGHARGVAMVAAASHRVKICEYSAREIKQSVVGYGAAEK
CHHHHHHHHHHHCCCCCHHEEECCCCCCHHEECCCCCEEHHHHHHHHHHHHHHCCCCCCH
DQVSHMVVELLQLNRAPQKDAADALAIAICHSHMRNGLSKLIGSRGTKRRRMRL
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCC
>Mature Secondary Structure 
TIILGIDPGSRVTGYGLIKESDRKIAYIDSGCIRTSNDVELSHKLLQIYDGICELMDHY
EEEEECCCCCCEEEEEEEECCCCEEEEEECCCEECCCCHHHHHHHHHHHHHHHHHHHCC
SPTEVAIEQIFMHNNPNSALKLGHARGVAMVAAASHRVKICEYSAREIKQSVVGYGAAEK
CHHHHHHHHHHHCCCCCHHEEECCCCCCHHEECCCCCEEHHHHHHHHHHHHHHCCCCCCH
DQVSHMVVELLQLNRAPQKDAADALAIAICHSHMRNGLSKLIGSRGTKRRRMRL
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA