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The map label for this gene is ribA [H]

Identifier: 54294124

GI number: 54294124

Start: 1326769

End: 1327977

Strand: Direct

Name: ribA [H]

Synonym: lpl1188

Alternate gene names: 54294124

Gene position: 1326769-1327977 (Clockwise)

Preceding gene: 54294123

Following gene: 54294125

Centisome position: 39.66

GC content: 40.53

Gene sequence:

>1209_bases
ATGAAACATTCATTAGCAACAATAGAAGAGGCTGTGGCGACACTTAAAGCCGGTAAAATGATTATTCTAATGGATGATGA
AGATAGAGAGAATGAAGGTGATTTGGTGATTGCTGCTGAGCACGCTACTCCCGAAGCTATTAATTTTATGTCGCGTCTTG
GTTGTGGTTTAATTTGTTTGCCTATGGCAGAGGAATTGATAGATAAGCTTGAGTTGCCCATGATGGCTCGGCATAACAAA
TCGCCGTATGGAACGGCGTTTACAGTTTCGATTGAAGCAGCTAATGGTGTCTCCACAGGCATCTCAGCAAGAGACAGGGC
TCGAACAATTCAAGTAGCTATTGATCCAAAAAGCGGGCCATCTGATATCATTTCACCTGGACATGTTTTTCCTTTACGGG
CTAGAAAGAGAGGAGTTTTAGAAAGACCGGGTCAAACAGAAGGCAGTGTTGATTTGGTAAAACTTGCCGGATTGACCCCG
GCCGCAGTGATTTGTGAAATTATTAATGAAGATGGAACAATGAGCAGACGTGATGAATTAGCTCTTTTTTCAAAAAAACA
TCAAATCCCTTTAGTCACCATTAAAGATTTAATTCAGTACAGAGTACGGCATGAAAATCTGATTCATCCCGTTGCAACCA
CACAAATTCCTCTGGAGGAGAACGGTGTTTTTACTATGACTGTTTTTGAGAATCAGATAGATTCCTGTGAACATTTTGTC
TTGGTTAAACCCCCTTTGTATGGTAATCAAGTGCCTTTGGTTCGAATTCATTCTGAATGCATAACAGGTGATGTTTTTGG
CTCCAGAAAATGTGATTGTGGAAAGCAACTGGAGTTGTCTTTATCCCAGATCGGAGCTGAAGGCGGTATATTAATTTATT
TGCGTCAGGAAGGCCGCGGAATAGGTTTGGCAAACAAATTAAAGGCGTATGCTTTGCAAGAGCAGGGGTTTGATACAGTC
GATGCTAATCTGGAATTAGGATTGCCCGCTGACGATAGAGATTATGCCGTAGCATACCAAATACTGAAGTATTTAGGGGT
TGAAGCATTAAGGTTGCTGACAAATAATCCTTTAAAAATCGCTTCTCTTGAGCGTTATGGAATGAAGATTACTCAACGCA
TTCCTTTGGAAATTGAGCCCTCCAGAGAAAATCACAGTTATTTAAAAACCAAAAAAATAAAACTGGGGCATTTATTAGCC
ATTGATTAG

Upstream 100 bases:

>100_bases
TGAGAGTGGGCCAGCAAGTCAATATTGAATTTGATTATCTTACCCGTATTGTGGCTCATCAGCTTCAAATTATCGGGAAA
TTGAACCAAGAGGAAGTGTC

Downstream 100 bases:

>100_bases
GATAAACATGAGAGAGATAAAAACAATTGTTAGTGATGGTGTCACGTTTCCAATAGCGCTAGTTGTAAGTACTTTTAATG
AACCGATAACCTCTGCATTA

Product: riboflavin biosynthesis protein RibA

Products: NA

Alternate protein names: 3,4-dihydroxy-2-butanone 4-phosphate synthase; DHBP synthase; GTP cyclohydrolase-2; GTP cyclohydrolase II [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MKHSLATIEEAVATLKAGKMIILMDDEDRENEGDLVIAAEHATPEAINFMSRLGCGLICLPMAEELIDKLELPMMARHNK
SPYGTAFTVSIEAANGVSTGISARDRARTIQVAIDPKSGPSDIISPGHVFPLRARKRGVLERPGQTEGSVDLVKLAGLTP
AAVICEIINEDGTMSRRDELALFSKKHQIPLVTIKDLIQYRVRHENLIHPVATTQIPLEENGVFTMTVFENQIDSCEHFV
LVKPPLYGNQVPLVRIHSECITGDVFGSRKCDCGKQLELSLSQIGAEGGILIYLRQEGRGIGLANKLKAYALQEQGFDTV
DANLELGLPADDRDYAVAYQILKYLGVEALRLLTNNPLKIASLERYGMKITQRIPLEIEPSRENHSYLKTKKIKLGHLLA
ID

Sequences:

>Translated_402_residues
MKHSLATIEEAVATLKAGKMIILMDDEDRENEGDLVIAAEHATPEAINFMSRLGCGLICLPMAEELIDKLELPMMARHNK
SPYGTAFTVSIEAANGVSTGISARDRARTIQVAIDPKSGPSDIISPGHVFPLRARKRGVLERPGQTEGSVDLVKLAGLTP
AAVICEIINEDGTMSRRDELALFSKKHQIPLVTIKDLIQYRVRHENLIHPVATTQIPLEENGVFTMTVFENQIDSCEHFV
LVKPPLYGNQVPLVRIHSECITGDVFGSRKCDCGKQLELSLSQIGAEGGILIYLRQEGRGIGLANKLKAYALQEQGFDTV
DANLELGLPADDRDYAVAYQILKYLGVEALRLLTNNPLKIASLERYGMKITQRIPLEIEPSRENHSYLKTKKIKLGHLLA
ID
>Mature_402_residues
MKHSLATIEEAVATLKAGKMIILMDDEDRENEGDLVIAAEHATPEAINFMSRLGCGLICLPMAEELIDKLELPMMARHNK
SPYGTAFTVSIEAANGVSTGISARDRARTIQVAIDPKSGPSDIISPGHVFPLRARKRGVLERPGQTEGSVDLVKLAGLTP
AAVICEIINEDGTMSRRDELALFSKKHQIPLVTIKDLIQYRVRHENLIHPVATTQIPLEENGVFTMTVFENQIDSCEHFV
LVKPPLYGNQVPLVRIHSECITGDVFGSRKCDCGKQLELSLSQIGAEGGILIYLRQEGRGIGLANKLKAYALQEQGFDTV
DANLELGLPADDRDYAVAYQILKYLGVEALRLLTNNPLKIASLERYGMKITQRIPLEIEPSRENHSYLKTKKIKLGHLLA
ID

Specific function: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate [H]

COG id: COG0108

COG function: function code H; 3,4-dihydroxy-2-butanone 4-phosphate synthase

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the GTP cyclohydrolase II family [H]

Homologues:

Organism=Escherichia coli, GI1789420, Length=198, Percent_Identity=51.5151515151515, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI1787533, Length=190, Percent_Identity=49.4736842105263, Blast_Score=180, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6320695, Length=201, Percent_Identity=44.7761194029851, Blast_Score=186, Evalue=7e-48,
Organism=Saccharomyces cerevisiae, GI6319438, Length=170, Percent_Identity=45.2941176470588, Blast_Score=129, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017945
- InterPro:   IPR000422
- InterPro:   IPR000926
- InterPro:   IPR016299 [H]

Pfam domain/function: PF00926 DHBP_synthase; PF00925 GTP_cyclohydro2 [H]

EC number: =4.1.99.12; =3.5.4.25 [H]

Molecular weight: Translated: 44320; Mature: 44320

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHSLATIEEAVATLKAGKMIILMDDEDRENEGDLVIAAEHATPEAINFMSRLGCGLICL
CCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCEEEH
PMAEELIDKLELPMMARHNKSPYGTAFTVSIEAANGVSTGISARDRARTIQVAIDPKSGP
HHHHHHHHHHCCCHHHHCCCCCCCEEEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCC
SDIISPGHVFPLRARKRGVLERPGQTEGSVDLVKLAGLTPAAVICEIINEDGTMSRRDEL
HHCCCCCCEEEECHHHCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCHHHH
ALFSKKHQIPLVTIKDLIQYRVRHENLIHPVATTQIPLEENGVFTMTVFENQIDSCEHFV
HHHHHCCCCCEEEHHHHHHHHHHHCCCCCCCCCEECEECCCCEEEEEEECCCCCCCCEEE
LVKPPLYGNQVPLVRIHSECITGDVFGSRKCDCGKQLELSLSQIGAEGGILIYLRQEGRG
EECCCCCCCCCCEEEEECHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEECCCCC
IGLANKLKAYALQEQGFDTVDANLELGLPADDRDYAVAYQILKYLGVEALRLLTNNPLKI
CCHHHHHHHHHHHHCCCCEECCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
ASLERYGMKITQRIPLEIEPSRENHSYLKTKKIKLGHLLAID
EEHHHHCCEEEEECCEEECCCCCCCCEEEEEEEEECEEEEEC
>Mature Secondary Structure
MKHSLATIEEAVATLKAGKMIILMDDEDRENEGDLVIAAEHATPEAINFMSRLGCGLICL
CCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCEEEH
PMAEELIDKLELPMMARHNKSPYGTAFTVSIEAANGVSTGISARDRARTIQVAIDPKSGP
HHHHHHHHHHCCCHHHHCCCCCCCEEEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCC
SDIISPGHVFPLRARKRGVLERPGQTEGSVDLVKLAGLTPAAVICEIINEDGTMSRRDEL
HHCCCCCCEEEECHHHCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCHHHH
ALFSKKHQIPLVTIKDLIQYRVRHENLIHPVATTQIPLEENGVFTMTVFENQIDSCEHFV
HHHHHCCCCCEEEHHHHHHHHHHHCCCCCCCCCEECEECCCCEEEEEEECCCCCCCCEEE
LVKPPLYGNQVPLVRIHSECITGDVFGSRKCDCGKQLELSLSQIGAEGGILIYLRQEGRG
EECCCCCCCCCCEEEEECHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEECCCCC
IGLANKLKAYALQEQGFDTVDANLELGLPADDRDYAVAYQILKYLGVEALRLLTNNPLKI
CCHHHHHHHHHHHHCCCCEECCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
ASLERYGMKITQRIPLEIEPSRENHSYLKTKKIKLGHLLAID
EEHHHHCCEEEEECCEEECCCCCCCCEEEEEEEEECEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA