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The map label for this gene is birA [H]

Identifier: 54293766

GI number: 54293766

Start: 930867

End: 931868

Strand: Reverse

Name: birA [H]

Synonym: lpl0822

Alternate gene names: 54293766

Gene position: 931868-930867 (Counterclockwise)

Preceding gene: 54293767

Following gene: 54293765

Centisome position: 27.85

GC content: 36.23

Gene sequence:

>1002_bases
ATGTTTCAATTTAATACTTCTCAACATACTTTAATGCAAATACTGGGCGATGGGGCATGTCATAGTGGGAGTGAATTAGG
AAACGCTTTAAAAATCAGTCGCTCAGCAGTATGGAAACAAATCAATCAGCTTAATGATTTAGGCATACCTATTATCCGCA
TACCACATCAAGGATACCAATTGGAAAAGCCTTTAATCCTGCTAGACTCTGATGAAATTACCAAGCAATTACACAGCAAA
GGATTTAGCCAACCGTTTAACCTGCATTTATTTACATCGATCGACTCAACCAACCGCTACCTGAAAGATCTGCCCTTAAG
CTCAGCCGTCGAAATTTGCTGCACTGAAATGCAAACTCAAGGGAGAGGGCGCTTTGGACGACACTGGCACTCTCCTTTTG
GAGAGAATATTTACTGCTCTACCCGGTGGAGTTTTAGTTGTGACATATCTAAGCTATCGGGTCTCAGTTTAGTCATAAGT
TTAGCAACAGTTCTCACTATAAATGAATTCATTGTATCTGATTCTGATAAAGTAAAAATAAAATGGCCAAATGATATCCT
GTGGAATAATAAAAAGTTATGTGGGATTTTAGTAGAGATTATTGCAGAATCTAACGCAACAGCACAATTTATTATAGGTG
TTGGTTTAAATATCAACTCTGATACAAAAAACAATCCTCTACCAGATAAATCCTGGTGTTCATTTTTTGAAATAACTAAT
CAAAATTACAATCGGAATTCGATTGTTACAAGGCTATTAATTAACCTGGAAAAATATCTATCAAAATTTATTAATAACGA
TCTAAATGCATTTAAGGATGACTGGAATAAGTATGACTACCTTGTTGGAAAAAAAGTTAAAGTGTCCCAAGCTTTGAATT
CGATTTCAGGGATTGCCTTGGGCATTAATTCGTTTGGCCAACTGATTCTAGAAGATGAATTTGGAAATAAGCATGAGCTC
TCTTCGGGCGATACATCACTTGCCCTAAGTAATTTTGAATAG

Upstream 100 bases:

>100_bases
AGTATGCTGCAGTGGCGCTTTATGCTACCACCCTTCTGTTAGAGAAATTCGTCATGGGATCGTCCAGCTTCAACAAAATA
TTAGTTTAAAATTTTAAATC

Downstream 100 bases:

>100_bases
GGTTTTTAACAGAAAGGAAGTTTTAGAGTAAAATTTCAAATTTAATATTTTAATATTTTAATATTTTAATATTTTAATAT
TTTAATATTTTAATATTTTA

Product: biotin-[acetylCoA carboxylase] holoenzyme synthetase and biotin operon repressor

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MFQFNTSQHTLMQILGDGACHSGSELGNALKISRSAVWKQINQLNDLGIPIIRIPHQGYQLEKPLILLDSDEITKQLHSK
GFSQPFNLHLFTSIDSTNRYLKDLPLSSAVEICCTEMQTQGRGRFGRHWHSPFGENIYCSTRWSFSCDISKLSGLSLVIS
LATVLTINEFIVSDSDKVKIKWPNDILWNNKKLCGILVEIIAESNATAQFIIGVGLNINSDTKNNPLPDKSWCSFFEITN
QNYNRNSIVTRLLINLEKYLSKFINNDLNAFKDDWNKYDYLVGKKVKVSQALNSISGIALGINSFGQLILEDEFGNKHEL
SSGDTSLALSNFE

Sequences:

>Translated_333_residues
MFQFNTSQHTLMQILGDGACHSGSELGNALKISRSAVWKQINQLNDLGIPIIRIPHQGYQLEKPLILLDSDEITKQLHSK
GFSQPFNLHLFTSIDSTNRYLKDLPLSSAVEICCTEMQTQGRGRFGRHWHSPFGENIYCSTRWSFSCDISKLSGLSLVIS
LATVLTINEFIVSDSDKVKIKWPNDILWNNKKLCGILVEIIAESNATAQFIIGVGLNINSDTKNNPLPDKSWCSFFEITN
QNYNRNSIVTRLLINLEKYLSKFINNDLNAFKDDWNKYDYLVGKKVKVSQALNSISGIALGINSFGQLILEDEFGNKHEL
SSGDTSLALSNFE
>Mature_333_residues
MFQFNTSQHTLMQILGDGACHSGSELGNALKISRSAVWKQINQLNDLGIPIIRIPHQGYQLEKPLILLDSDEITKQLHSK
GFSQPFNLHLFTSIDSTNRYLKDLPLSSAVEICCTEMQTQGRGRFGRHWHSPFGENIYCSTRWSFSCDISKLSGLSLVIS
LATVLTINEFIVSDSDKVKIKWPNDILWNNKKLCGILVEIIAESNATAQFIIGVGLNINSDTKNNPLPDKSWCSFFEITN
QNYNRNSIVTRLLINLEKYLSKFINNDLNAFKDDWNKYDYLVGKKVKVSQALNSISGIALGINSFGQLILEDEFGNKHEL
SSGDTSLALSNFE

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=311, Percent_Identity=34.7266881028939, Blast_Score=189, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR004409
- InterPro:   IPR003142
- InterPro:   IPR004143
- InterPro:   IPR013196
- InterPro:   IPR008988
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB; PF08279 HTH_11 [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 37444; Mature: 37444

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQFNTSQHTLMQILGDGACHSGSELGNALKISRSAVWKQINQLNDLGIPIIRIPHQGYQ
CCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCC
LEKPLILLDSDEITKQLHSKGFSQPFNLHLFTSIDSTNRYLKDLPLSSAVEICCTEMQTQ
CCCCEEEECCHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHCCHHHHHHHHHHHHHHC
GRGRFGRHWHSPFGENIYCSTRWSFSCDISKLSGLSLVISLATVLTINEFIVSDSDKVKI
CCCCCCCCCCCCCCCCEEEEECCCEEECHHHHCHHHHHHHHHHHHHHHHHHCCCCCEEEE
KWPNDILWNNKKLCGILVEIIAESNATAQFIIGVGLNINSDTKNNPLPDKSWCSFFEITN
ECCCCEEECCCHHHHHHHHHHHCCCCCEEEEEEECEEECCCCCCCCCCCHHHHHHHHCCC
QNYNRNSIVTRLLINLEKYLSKFINNDLNAFKDDWNKYDYLVGKKVKVSQALNSISGIAL
CCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEE
GINSFGQLILEDEFGNKHELSSGDTSLALSNFE
EHHHHCCEEEECCCCCCCCCCCCCCEEEECCCC
>Mature Secondary Structure
MFQFNTSQHTLMQILGDGACHSGSELGNALKISRSAVWKQINQLNDLGIPIIRIPHQGYQ
CCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCC
LEKPLILLDSDEITKQLHSKGFSQPFNLHLFTSIDSTNRYLKDLPLSSAVEICCTEMQTQ
CCCCEEEECCHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHCCHHHHHHHHHHHHHHC
GRGRFGRHWHSPFGENIYCSTRWSFSCDISKLSGLSLVISLATVLTINEFIVSDSDKVKI
CCCCCCCCCCCCCCCCEEEEECCCEEECHHHHCHHHHHHHHHHHHHHHHHHCCCCCEEEE
KWPNDILWNNKKLCGILVEIIAESNATAQFIIGVGLNINSDTKNNPLPDKSWCSFFEITN
ECCCCEEECCCHHHHHHHHHHHCCCCCEEEEEEECEEECCCCCCCCCCCHHHHHHHHCCC
QNYNRNSIVTRLLINLEKYLSKFINNDLNAFKDDWNKYDYLVGKKVKVSQALNSISGIAL
CCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEE
GINSFGQLILEDEFGNKHELSSGDTSLALSNFE
EHHHHCCEEEECCCCCCCCCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3899863; 3536662; 8265357; 9278503; 1311302; 1328157; 1409631; 11353844 [H]