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The map label for this gene is artJ [H]

Identifier: 54293661

GI number: 54293661

Start: 800590

End: 801342

Strand: Reverse

Name: artJ [H]

Synonym: lpl0714

Alternate gene names: 54293661

Gene position: 801342-800590 (Counterclockwise)

Preceding gene: 54293668

Following gene: 54293659

Centisome position: 23.95

GC content: 38.38

Gene sequence:

>753_bases
ATGAAATTTATTCAGGGATTATTGCTTTCTTTCTTTTTTCTAGTTTCACCTCTCCACGCTCAAAACGAGCCTATACCTTT
AATAGTAGGTGTAGGAGGCTTTTACCCTCCCTTTATCATGCAAGGAACCAATAATGAGCTTTTTGGCTATGATATCGCCA
CCATGACAGAACTCTGTAAGATAATGAAACGCCAATGTAATTTTCAAGTCATGAGATTCAGACAATTAATTCCTGCTCTG
ATTAACAAACAAATTGATGTGGCGATCAGCGCCATGTCTATTACTTCAGAACGTTCCAGCCTGGTTAATTTCAGCATGCC
CTATTTATTGAGTTACTCCAGGTTTCTTACAAATAAGATACACAATACTCATCAACCGTTTAATTTAATGCTCTTAAAAG
GTAAACGAATTGGAGTGGAACAAAATACTGTCTTTAGTCAGGAAATCAGAAGGATGGGGATAATAGACCCTGTCATAGTG
GAGTTTGACAGAACCGAAGAAATGCTTGAGGCGTTGAGCGCAAATAAAATTGACTTTGTTCTGATGGATAATCCTGCGGC
TTTATACTGGTCCGCTAATTCGTCGGGAAGACTTCTGGTAATAGGCAGTCCCATGTTGGTTGGTTATGGTTTGGGAATTG
CAGTCAATCGGGAAAACTCCGTATTACTCAAATCTCTGAATGATGCCTTGGTAGAATACCAACGTTCTGACAAATACAAG
GAAAACTACAATAAATACATGATGCATTTTTAA

Upstream 100 bases:

>100_bases
CATAGGATTAATCAATTAATTTTACATTTAATACTTTAAAACTCCTATTGGACGTTATACTCTTATAAAAGACTCTAATA
ATATTTTAGTGAAGATACCA

Downstream 100 bases:

>100_bases
GAGTAATGTGGTTGGGGCAATTGCTACAAAAATTGCTCTTAATCATTCATCAATAGCTGAATAATCGTTTTAAGGCGTAA
TAAGTCAACGCCCTGATATT

Product: hypothetical protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MKFIQGLLLSFFFLVSPLHAQNEPIPLIVGVGGFYPPFIMQGTNNELFGYDIATMTELCKIMKRQCNFQVMRFRQLIPAL
INKQIDVAISAMSITSERSSLVNFSMPYLLSYSRFLTNKIHNTHQPFNLMLLKGKRIGVEQNTVFSQEIRRMGIIDPVIV
EFDRTEEMLEALSANKIDFVLMDNPAALYWSANSSGRLLVIGSPMLVGYGLGIAVNRENSVLLKSLNDALVEYQRSDKYK
ENYNKYMMHF

Sequences:

>Translated_250_residues
MKFIQGLLLSFFFLVSPLHAQNEPIPLIVGVGGFYPPFIMQGTNNELFGYDIATMTELCKIMKRQCNFQVMRFRQLIPAL
INKQIDVAISAMSITSERSSLVNFSMPYLLSYSRFLTNKIHNTHQPFNLMLLKGKRIGVEQNTVFSQEIRRMGIIDPVIV
EFDRTEEMLEALSANKIDFVLMDNPAALYWSANSSGRLLVIGSPMLVGYGLGIAVNRENSVLLKSLNDALVEYQRSDKYK
ENYNKYMMHF
>Mature_250_residues
MKFIQGLLLSFFFLVSPLHAQNEPIPLIVGVGGFYPPFIMQGTNNELFGYDIATMTELCKIMKRQCNFQVMRFRQLIPAL
INKQIDVAISAMSITSERSSLVNFSMPYLLSYSRFLTNKIHNTHQPFNLMLLKGKRIGVEQNTVFSQEIRRMGIIDPVIV
EFDRTEEMLEALSANKIDFVLMDNPAALYWSANSSGRLLVIGSPMLVGYGLGIAVNRENSVLLKSLNDALVEYQRSDKYK
ENYNKYMMHF

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=244, Percent_Identity=34.4262295081967, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788228, Length=234, Percent_Identity=28.6324786324786, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1787088, Length=218, Percent_Identity=30.2752293577982, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI1788648, Length=232, Percent_Identity=31.0344827586207, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI1788649, Length=251, Percent_Identity=28.2868525896414, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1787031, Length=240, Percent_Identity=27.5, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28572; Mature: 28572

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFIQGLLLSFFFLVSPLHAQNEPIPLIVGVGGFYPPFIMQGTNNELFGYDIATMTELCK
CHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEEEECCCCCEEECHHHHHHHHHH
IMKRQCNFQVMRFRQLIPALINKQIDVAISAMSITSERSSLVNFSMPYLLSYSRFLTNKI
HHHHHCCHHHHHHHHHHHHHHCCCHHEEEEEEEEHHHHHHHHCCCCHHHHHHHHHHHHHH
HNTHQPFNLMLLKGKRIGVEQNTVFSQEIRRMGIIDPVIVEFDRTEEMLEALSANKIDFV
CCCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCCHHEEEECCHHHHHHHHCCCCEEEE
LMDNPAALYWSANSSGRLLVIGSPMLVGYGLGIAVNRENSVLLKSLNDALVEYQRSDKYK
EECCCEEEEEECCCCCEEEEEECCHHEEECCEEEECCCCCHHHHHHHHHHHHHHHCCHHH
ENYNKYMMHF
HHHHHHCCCC
>Mature Secondary Structure
MKFIQGLLLSFFFLVSPLHAQNEPIPLIVGVGGFYPPFIMQGTNNELFGYDIATMTELCK
CHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEEEECCCCCEEECHHHHHHHHHH
IMKRQCNFQVMRFRQLIPALINKQIDVAISAMSITSERSSLVNFSMPYLLSYSRFLTNKI
HHHHHCCHHHHHHHHHHHHHHCCCHHEEEEEEEEHHHHHHHHCCCCHHHHHHHHHHHHHH
HNTHQPFNLMLLKGKRIGVEQNTVFSQEIRRMGIIDPVIVEFDRTEEMLEALSANKIDFV
CCCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCCHHEEEECCHHHHHHHHCCCCEEEE
LMDNPAALYWSANSSGRLLVIGSPMLVGYGLGIAVNRENSVLLKSLNDALVEYQRSDKYK
EECCCEEEEEECCCCCEEEEEECCHHEEECCEEEECCCCCHHHHHHHHHHHHHHHCCHHH
ENYNKYMMHF
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]