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The map label for this gene is ytbE [H]

Identifier: 54293507

GI number: 54293507

Start: 604443

End: 605273

Strand: Reverse

Name: ytbE [H]

Synonym: lpl0556

Alternate gene names: 54293507

Gene position: 605273-604443 (Counterclockwise)

Preceding gene: 54293508

Following gene: 54293492

Centisome position: 18.09

GC content: 37.06

Gene sequence:

>831_bases
ATGGACCTTGGCTTTCCAACTTTTATATATGGAACCGCCTGGAAAGAAAAGCGTACAAGCGAACTGGTCGAGCTTGCATT
AACTACCGGATTTCAGGGTATAGATACTGCAAACCAAAGAAAACACTATTATGAAGAAGGTGTTGGATATGCCTTGCAAC
AATACTATAAAAAATCAGGTATCTTGCGTGATGATATTTTTTTGCAATCCAAATTCACCTATGCTCATGGCCAGGATCAT
CGCAAACCTTACGATGATACAGCAAGCCTGACTATTCAAGTCCAGCAATCTTTTGCAAGCAGCCTTGAACATTTAAATAC
TGATTATTTAAATTCCTATATTTTGCATGGCCCTTATTATCAATATCATTTTTCTGATGAAGATAAAGAAGTCTGGAACA
AAATGGAATCCCTGCATAAAGATGAGAAAGTAAAACACCTGGGCATATCCAATGTGAGTTATGAACAGCTAGTCGAACTC
ATTTCATTTGCTTCGGTAAAACCCAAGTTTGTTCAAAATAGATGCTTTGCCAGGCTATTATGGGATAAACAAATTCGTAA
CTATTGCCAGGCTCATGAAATTATTTATCAGGGGTTTTCTCTGTTAACGGCCAATCAATATGAGTTAAGGCATCCGAAAC
TTGAGAATCTCAGCAAAAAGTACGAGAAATCGATTGAGCAAATCATTTTTAGATTTTCACAACAGATAGGAATGTTGCCT
TTAACTGGCACGACAAATTTGGATCACATGAAAGAAGATCTTGCCATCCATCAGTTCAGGCTGACTGATGATGAAATCAA
TTTCATTGAAAACATTGCCTTTATCCCATAA

Upstream 100 bases:

>100_bases
AAAACCCTATGCAAGACACACAGTCAGCTATGGTTTGCTCTGTTTTTTGAGTCAAAATAGAGTCTGTTTGCAGATAATAA
ATCGAGAAGGAGATTGAACG

Downstream 100 bases:

>100_bases
AATTTTTTTTCGATTTGACTGGGAAATTCATATTGTAATCCCCTGTAAAAATGGCATTCCCTAACCAAAAAAAATGAACT
TCATGATGAATGCTTCCTGA

Product: hypothetical protein

Products: 2-keto-L-gulonate; NADP [C]

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MDLGFPTFIYGTAWKEKRTSELVELALTTGFQGIDTANQRKHYYEEGVGYALQQYYKKSGILRDDIFLQSKFTYAHGQDH
RKPYDDTASLTIQVQQSFASSLEHLNTDYLNSYILHGPYYQYHFSDEDKEVWNKMESLHKDEKVKHLGISNVSYEQLVEL
ISFASVKPKFVQNRCFARLLWDKQIRNYCQAHEIIYQGFSLLTANQYELRHPKLENLSKKYEKSIEQIIFRFSQQIGMLP
LTGTTNLDHMKEDLAIHQFRLTDDEINFIENIAFIP

Sequences:

>Translated_276_residues
MDLGFPTFIYGTAWKEKRTSELVELALTTGFQGIDTANQRKHYYEEGVGYALQQYYKKSGILRDDIFLQSKFTYAHGQDH
RKPYDDTASLTIQVQQSFASSLEHLNTDYLNSYILHGPYYQYHFSDEDKEVWNKMESLHKDEKVKHLGISNVSYEQLVEL
ISFASVKPKFVQNRCFARLLWDKQIRNYCQAHEIIYQGFSLLTANQYELRHPKLENLSKKYEKSIEQIIFRFSQQIGMLP
LTGTTNLDHMKEDLAIHQFRLTDDEINFIENIAFIP
>Mature_276_residues
MDLGFPTFIYGTAWKEKRTSELVELALTTGFQGIDTANQRKHYYEEGVGYALQQYYKKSGILRDDIFLQSKFTYAHGQDH
RKPYDDTASLTIQVQQSFASSLEHLNTDYLNSYILHGPYYQYHFSDEDKEVWNKMESLHKDEKVKHLGISNVSYEQLVEL
ISFASVKPKFVQNRCFARLLWDKQIRNYCQAHEIIYQGFSLLTANQYELRHPKLENLSKKYEKSIEQIIFRFSQQIGMLP
LTGTTNLDHMKEDLAIHQFRLTDDEINFIENIAFIP

Specific function: Reduction [C]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI5174695, Length=305, Percent_Identity=25.5737704918033, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI223468663, Length=293, Percent_Identity=25.938566552901, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI45446745, Length=298, Percent_Identity=25.1677852348993, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI4503285, Length=298, Percent_Identity=25.1677852348993, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI310109920, Length=298, Percent_Identity=24.8322147651007, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI5453543, Length=298, Percent_Identity=24.8322147651007, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI291291012, Length=280, Percent_Identity=25.3571428571429, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI24497585, Length=297, Percent_Identity=25.5892255892256, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI24497583, Length=298, Percent_Identity=24.1610738255034, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI310109922, Length=283, Percent_Identity=25.0883392226148, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI24497577, Length=292, Percent_Identity=24.6575342465753, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI5174391, Length=292, Percent_Identity=24.6575342465753, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI300116273, Length=283, Percent_Identity=24.3816254416961, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4502049, Length=287, Percent_Identity=22.9965156794425, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI87082198, Length=251, Percent_Identity=26.6932270916335, Blast_Score=86, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71998625, Length=250, Percent_Identity=28.8, Blast_Score=101, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17564128, Length=303, Percent_Identity=27.3927392739274, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17562292, Length=261, Percent_Identity=26.0536398467433, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17566692, Length=296, Percent_Identity=23.3108108108108, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17561298, Length=257, Percent_Identity=26.8482490272374, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17537079, Length=305, Percent_Identity=23.9344262295082, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17552492, Length=274, Percent_Identity=23.3576642335766, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17538386, Length=265, Percent_Identity=24.9056603773585, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17561300, Length=261, Percent_Identity=23.7547892720307, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17537077, Length=257, Percent_Identity=24.124513618677, Blast_Score=68, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6320079, Length=255, Percent_Identity=29.0196078431373, Blast_Score=89, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6322556, Length=268, Percent_Identity=23.5074626865672, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324694, Length=296, Percent_Identity=25, Blast_Score=70, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320576, Length=296, Percent_Identity=25, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24657054, Length=285, Percent_Identity=27.0175438596491, Blast_Score=94, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24663317, Length=292, Percent_Identity=24.6575342465753, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24662789, Length=299, Percent_Identity=26.4214046822742, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI20129731, Length=286, Percent_Identity=23.7762237762238, Blast_Score=87, Evalue=9e-18,
Organism=Drosophila melanogaster, GI21356425, Length=261, Percent_Identity=24.904214559387, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI45553081, Length=286, Percent_Identity=27.6223776223776, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI281366140, Length=260, Percent_Identity=27.3076923076923, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: NA

Molecular weight: Translated: 32480; Mature: 32480

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLGFPTFIYGTAWKEKRTSELVELALTTGFQGIDTANQRKHYYEEGVGYALQQYYKKSG
CCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
ILRDDIFLQSKFTYAHGQDHRKPYDDTASLTIQVQQSFASSLEHLNTDYLNSYILHGPYY
CCHHHHHHHHCCCCCCCCCCCCCCCCCHHEEEEEHHHHHHHHHHHCHHHHHHHHCCCCEE
QYHFSDEDKEVWNKMESLHKDEKVKHLGISNVSYEQLVELISFASVKPKFVQNRCFARLL
EEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
WDKQIRNYCQAHEIIYQGFSLLTANQYELRHPKLENLSKKYEKSIEQIIFRFSQQIGMLP
HHHHHHHHHHHHHHHHHCHHHHCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEE
LTGTTNLDHMKEDLAIHQFRLTDDEINFIENIAFIP
CCCCCCHHHHHHHHHHHHEECCCHHHHHHHHHCCCC
>Mature Secondary Structure
MDLGFPTFIYGTAWKEKRTSELVELALTTGFQGIDTANQRKHYYEEGVGYALQQYYKKSG
CCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
ILRDDIFLQSKFTYAHGQDHRKPYDDTASLTIQVQQSFASSLEHLNTDYLNSYILHGPYY
CCHHHHHHHHCCCCCCCCCCCCCCCCCHHEEEEEHHHHHHHHHHHCHHHHHHHHCCCCEE
QYHFSDEDKEVWNKMESLHKDEKVKHLGISNVSYEQLVELISFASVKPKFVQNRCFARLL
EEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
WDKQIRNYCQAHEIIYQGFSLLTANQYELRHPKLENLSKKYEKSIEQIIFRFSQQIGMLP
HHHHHHHHHHHHHHHHHCHHHHCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEE
LTGTTNLDHMKEDLAIHQFRLTDDEINFIENIAFIP
CCCCCCHHHHHHHHHHHHEECCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,5-diketo-D-gluconate; NADPH [C]

Specific reaction: 2,5-diketo-D-gluconate + NADPH = 2-keto-L-gulonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]