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The map label for this gene is trmD [H]

Identifier: 54293391

GI number: 54293391

Start: 485953

End: 486705

Strand: Reverse

Name: trmD [H]

Synonym: lpl0440

Alternate gene names: 54293391

Gene position: 486705-485953 (Counterclockwise)

Preceding gene: 54293392

Following gene: 54293390

Centisome position: 14.55

GC content: 42.5

Gene sequence:

>753_bases
GTGGCATTACATCTTGGAATCATCACCCTGTTGCCTGAAATAATACAAGGCATCCATTATGGGGTAACCGGCCGAGCTAT
TGAACAAGGCTTGGTTAAAATTGATTGCTGGAATCCTAGGGATTGGTCATCAAGACCTTATAAGCAAGTGGATGACAAGC
CCTATGGTGGTGGACCAGGAATGGTCATAATGTATGAGCCATTGCATGCTGCAATAAAGCATGCGCGCAGTGAAATGAAG
GAAAACTGCAAAACAATTTACTTAAGCCCTCAAGGCAAAGTAGTTAGACAGAACGATTTAAAACAAATAGCAGCCCAAAA
ACAATCCTTGCTATTTGTTGCAGGGAGATATGAAGGAATTGATGAGCGAATCATTAGTCACCACGTAGATGAAGAATGGT
CGCTAGGGGATTTTGTATTAAGTGGCGGTGAATTGGCCGCAATGGTATTTATTGATGCGATAATTCGCTTGATACCTGGA
AGCCTTGGGCATTTGGGTTCAGCAGTACAAGATTCATTTATGAATGGATTACTGGACTGCCCACATTATACTCGGCCAGC
AACTATAAATGGACTGGATGTACCTGATGTTTTATTAGGAGGAAACCATAAAGAAATAGAACGCTGGCGTAGGAAACAAT
CCCTGGGTAAAACCTGGCTCAAACGCCCGGATTTACTTGAGAAAGTACAATTAAGTGAAACAGACAAGCAATTGCTTGCT
GAGTTTAAATGCGAACACGGCGATTCCTGCTGA

Upstream 100 bases:

>100_bases
GTGAAAAAAGGCATTTAATCCCCTATCTTCCAGGACAATTTGTAATCAATATTGATGAAAGCCAGCAAGTAATCACTGTT
GATTGGGATATGAATTTTTA

Downstream 100 bases:

>100_bases
GGATGAGCCAATTTGAGGAGTAGTCTATGACTAATATTATTGACCAAATTAATGCCGAACAAATGCAAGGCAAAGAAATT
CCTGATTTCAACCCAGGCGA

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MALHLGIITLLPEIIQGIHYGVTGRAIEQGLVKIDCWNPRDWSSRPYKQVDDKPYGGGPGMVIMYEPLHAAIKHARSEMK
ENCKTIYLSPQGKVVRQNDLKQIAAQKQSLLFVAGRYEGIDERIISHHVDEEWSLGDFVLSGGELAAMVFIDAIIRLIPG
SLGHLGSAVQDSFMNGLLDCPHYTRPATINGLDVPDVLLGGNHKEIERWRRKQSLGKTWLKRPDLLEKVQLSETDKQLLA
EFKCEHGDSC

Sequences:

>Translated_250_residues
MALHLGIITLLPEIIQGIHYGVTGRAIEQGLVKIDCWNPRDWSSRPYKQVDDKPYGGGPGMVIMYEPLHAAIKHARSEMK
ENCKTIYLSPQGKVVRQNDLKQIAAQKQSLLFVAGRYEGIDERIISHHVDEEWSLGDFVLSGGELAAMVFIDAIIRLIPG
SLGHLGSAVQDSFMNGLLDCPHYTRPATINGLDVPDVLLGGNHKEIERWRRKQSLGKTWLKRPDLLEKVQLSETDKQLLA
EFKCEHGDSC
>Mature_249_residues
ALHLGIITLLPEIIQGIHYGVTGRAIEQGLVKIDCWNPRDWSSRPYKQVDDKPYGGGPGMVIMYEPLHAAIKHARSEMKE
NCKTIYLSPQGKVVRQNDLKQIAAQKQSLLFVAGRYEGIDERIISHHVDEEWSLGDFVLSGGELAAMVFIDAIIRLIPGS
LGHLGSAVQDSFMNGLLDCPHYTRPATINGLDVPDVLLGGNHKEIERWRRKQSLGKTWLKRPDLLEKVQLSETDKQLLAE
FKCEHGDSC

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=244, Percent_Identity=57.3770491803279, Blast_Score=287, Evalue=4e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 27989; Mature: 27857

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALHLGIITLLPEIIQGIHYGVTGRAIEQGLVKIDCWNPRDWSSRPYKQVDDKPYGGGPG
CCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCC
MVIMYEPLHAAIKHARSEMKENCKTIYLSPQGKVVRQNDLKQIAAQKQSLLFVAGRYEGI
EEEEECHHHHHHHHHHHHHHCCCCEEEECCCCCEECHHHHHHHHHHHHHEEEEECCCCCH
DERIISHHVDEEWSLGDFVLSGGELAAMVFIDAIIRLIPGSLGHLGSAVQDSFMNGLLDC
HHHHHHHHCCCCCCCHHHEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCC
PHYTRPATINGLDVPDVLLGGNHKEIERWRRKQSLGKTWLKRPDLLEKVQLSETDKQLLA
CCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
EFKCEHGDSC
HHHHCCCCCC
>Mature Secondary Structure 
ALHLGIITLLPEIIQGIHYGVTGRAIEQGLVKIDCWNPRDWSSRPYKQVDDKPYGGGPG
CCHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCC
MVIMYEPLHAAIKHARSEMKENCKTIYLSPQGKVVRQNDLKQIAAQKQSLLFVAGRYEGI
EEEEECHHHHHHHHHHHHHHCCCCEEEECCCCCEECHHHHHHHHHHHHHEEEEECCCCCH
DERIISHHVDEEWSLGDFVLSGGELAAMVFIDAIIRLIPGSLGHLGSAVQDSFMNGLLDC
HHHHHHHHCCCCCCCHHHEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCC
PHYTRPATINGLDVPDVLLGGNHKEIERWRRKQSLGKTWLKRPDLLEKVQLSETDKQLLA
CCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
EFKCEHGDSC
HHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA