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The map label for this gene is ycdT [H]

Identifier: 54293235

GI number: 54293235

Start: 316262

End: 318391

Strand: Reverse

Name: ycdT [H]

Synonym: lpl0283

Alternate gene names: 54293235

Gene position: 318391-316262 (Counterclockwise)

Preceding gene: 54293236

Following gene: 54293234

Centisome position: 9.52

GC content: 39.11

Gene sequence:

>2130_bases
ATGAATAGCACTTGGCATCAAACCGCCGCTGGAAACCGCTCCATTTTAAGTAATTTGCGATTAAGCAGAACTCTACTTAA
CGTCATCTTTATTATTGCCCTTTCCCTGCTTTTAATCATCAATATTATTTCTTATAACCAGGTCAAAAATTTAATCTTCG
CCAGTAACTGGGTTTCTCATAGTAATGAGGTCATCCAAACCATAGATACATGCCTTTATGATGTAGTAGAAATTGAATCT
CGCCAGAGATTTTACTTGATTCGAGGTTCAAGCCCTCAGTTTATGACTGATGTAGATGATATGAAATCAACATTACAGCT
GAATTTAGACAAACTTGAGCAATTAACCAAGGACAACCCAGAACAAAACAAACGAACCCATCGTTTTATTGATTTGGTTA
AACAAAGATTAAATTTGTTAAACCAACTTGTACAGTTAAAGGGAAGCGGCAAACTCGATACACAAGAAGGTTATGATTTA
CTTAACCAAAGTCAAGACATGTCGCATCAAGTTAAAAGCCTTGGACAAGAAATTAAATCTATTGAATTGGTGTTGCTATC
AGAAAGAAATACTGGTGCGGTTCGCTGGGCAGATACCAGTAGTTTTATTTTAATATCCGGAGGTATTACAAGCATCCTGT
TTTTAACGATCGCTTTTATTCTGGCAAATATTGAATTGTCAACACGCAGAAAAACTGAACTACAGAATCAAAATACGCAA
ATACGATTGAAGAAAATTATAGAAAGTGCTAGTGATATGATCGCCGCTTTTGACAAAGACCAGCGATTTATCACTTTTAA
TGAAGCTTATCAGCGTGAGTTTAAACGTCTTTTTGATAAATCCATAAGCATCAATATGTCTCTGGAAGAAGCGTTCGATG
ATGTTCCTGAAAACAAAAAAAAATTGGTGCAAACCTGGAAAGAATCTCTACAAAGGGATGAAGAAACTAAAAATATCGAA
GTGAATACAGAACAGGAAAAGACTATTTATGAGATGACCTCCAAACTCATCCAAAACGGTGACAATGAAATTAAAGGTGT
GGTACACAGCGTTCGCAATATTACCAAACGGGTACAAGAACATACCGAACTACAAGAATCGTATGAAAAACTGGCTAATG
GAATGAAAGAACTGCAAGAGAAAAATGAACAAATTACCCTGCTGGTTGAGATGAGTGACATCATGCTGGCTTGTGGCTCC
CAGGAAGAGCTAAGCGACGTAATGGCGAAATACTCCCAACGACTACTGCAATTTTCAAGCGGCTACCTGTTTATCATGCA
TCCTTCCAAAAATTATTTGGAAAAAGCCGCCAGTTGGGGTAATCCTCAACCGCACGATCTGACTTTTACCCCGGAGCAAT
GTTGGGCTATCCGGCTTGGTCGAATTCATTATGCAGGTTCATCGCGTATCGAGTTGATGTGTAGTCATACGATGTTTGCC
GAGCAACCCGAGTTGTCTCTGCTTTGTGTACCCCTAATGGCCCAAAACGACATTTACGGCTTGTTATACCTTGAAGTTGG
CCTAAAATTTGATGAAAACCAACAATTACTAATAACTGAATTCGCAGAGCTTACCGCCTTGGCTTTGGCAAATGTCCGCC
TGAGAGAAAATTTACGCTATCAATCTATCCGTGATCCACTCACTGGATTATACAATCGCCGTTATCTGGAAGATTTTCTA
TTTAAACAATTACATCAGGCTGAACGAACAAAAGCCTCATTTGCCATTTTAATGCTGGATTTGGATCATTTTAAAAAGAT
CAACGATACCTTTGGTCATGACGCAGGAGATCTTGTATTAAAAGAGCTAGGGCAAATTTTGAACAGTGATATTCGCCTTG
GTGATATTGCAGCCCGATATGGGGGAGAAGAATTTGTCCTGCTGCTCTATGATATTGATGCTCAAGCCGCTAAAATGAAA
GCCGAAAATTTGCGTTCGGCCATTTCCAATTTACAGGTTAAATACGGAGCGCAACCTGTTGGGCAGATAACGGCCTCAAT
CGGAATATCCGTCTATCCTGACGATGCCAAATCACCTGCTGAAGTGATTGAAGCAGCTGATAAAGCTTTATACCAAGCCA
AAAACAAAGGCAGGAATAAGGTAATTTTATTCTCGGAAATATCATCCTGA

Upstream 100 bases:

>100_bases
GTTGTTACTCCTGATGCCTCATAAGTAACTGTTTTTTGGACTATACTCTTATCAAGACCATGCAAAGCATCAAGCCATAA
AATTAGGATGATTCAATGCC

Downstream 100 bases:

>100_bases
TTTGGATTGCAGCTCCTGACCTAAATGTTGTGATGTTTCTTATGTCCGATAGAGAGGTTCATGCTTTATATGAGTTTTGA
GAGCGAAACAATAGATACTA

Product: hypothetical protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 709; Mature: 709

Protein sequence:

>709_residues
MNSTWHQTAAGNRSILSNLRLSRTLLNVIFIIALSLLLIINIISYNQVKNLIFASNWVSHSNEVIQTIDTCLYDVVEIES
RQRFYLIRGSSPQFMTDVDDMKSTLQLNLDKLEQLTKDNPEQNKRTHRFIDLVKQRLNLLNQLVQLKGSGKLDTQEGYDL
LNQSQDMSHQVKSLGQEIKSIELVLLSERNTGAVRWADTSSFILISGGITSILFLTIAFILANIELSTRRKTELQNQNTQ
IRLKKIIESASDMIAAFDKDQRFITFNEAYQREFKRLFDKSISINMSLEEAFDDVPENKKKLVQTWKESLQRDEETKNIE
VNTEQEKTIYEMTSKLIQNGDNEIKGVVHSVRNITKRVQEHTELQESYEKLANGMKELQEKNEQITLLVEMSDIMLACGS
QEELSDVMAKYSQRLLQFSSGYLFIMHPSKNYLEKAASWGNPQPHDLTFTPEQCWAIRLGRIHYAGSSRIELMCSHTMFA
EQPELSLLCVPLMAQNDIYGLLYLEVGLKFDENQQLLITEFAELTALALANVRLRENLRYQSIRDPLTGLYNRRYLEDFL
FKQLHQAERTKASFAILMLDLDHFKKINDTFGHDAGDLVLKELGQILNSDIRLGDIAARYGGEEFVLLLYDIDAQAAKMK
AENLRSAISNLQVKYGAQPVGQITASIGISVYPDDAKSPAEVIEAADKALYQAKNKGRNKVILFSEISS

Sequences:

>Translated_709_residues
MNSTWHQTAAGNRSILSNLRLSRTLLNVIFIIALSLLLIINIISYNQVKNLIFASNWVSHSNEVIQTIDTCLYDVVEIES
RQRFYLIRGSSPQFMTDVDDMKSTLQLNLDKLEQLTKDNPEQNKRTHRFIDLVKQRLNLLNQLVQLKGSGKLDTQEGYDL
LNQSQDMSHQVKSLGQEIKSIELVLLSERNTGAVRWADTSSFILISGGITSILFLTIAFILANIELSTRRKTELQNQNTQ
IRLKKIIESASDMIAAFDKDQRFITFNEAYQREFKRLFDKSISINMSLEEAFDDVPENKKKLVQTWKESLQRDEETKNIE
VNTEQEKTIYEMTSKLIQNGDNEIKGVVHSVRNITKRVQEHTELQESYEKLANGMKELQEKNEQITLLVEMSDIMLACGS
QEELSDVMAKYSQRLLQFSSGYLFIMHPSKNYLEKAASWGNPQPHDLTFTPEQCWAIRLGRIHYAGSSRIELMCSHTMFA
EQPELSLLCVPLMAQNDIYGLLYLEVGLKFDENQQLLITEFAELTALALANVRLRENLRYQSIRDPLTGLYNRRYLEDFL
FKQLHQAERTKASFAILMLDLDHFKKINDTFGHDAGDLVLKELGQILNSDIRLGDIAARYGGEEFVLLLYDIDAQAAKMK
AENLRSAISNLQVKYGAQPVGQITASIGISVYPDDAKSPAEVIEAADKALYQAKNKGRNKVILFSEISS
>Mature_709_residues
MNSTWHQTAAGNRSILSNLRLSRTLLNVIFIIALSLLLIINIISYNQVKNLIFASNWVSHSNEVIQTIDTCLYDVVEIES
RQRFYLIRGSSPQFMTDVDDMKSTLQLNLDKLEQLTKDNPEQNKRTHRFIDLVKQRLNLLNQLVQLKGSGKLDTQEGYDL
LNQSQDMSHQVKSLGQEIKSIELVLLSERNTGAVRWADTSSFILISGGITSILFLTIAFILANIELSTRRKTELQNQNTQ
IRLKKIIESASDMIAAFDKDQRFITFNEAYQREFKRLFDKSISINMSLEEAFDDVPENKKKLVQTWKESLQRDEETKNIE
VNTEQEKTIYEMTSKLIQNGDNEIKGVVHSVRNITKRVQEHTELQESYEKLANGMKELQEKNEQITLLVEMSDIMLACGS
QEELSDVMAKYSQRLLQFSSGYLFIMHPSKNYLEKAASWGNPQPHDLTFTPEQCWAIRLGRIHYAGSSRIELMCSHTMFA
EQPELSLLCVPLMAQNDIYGLLYLEVGLKFDENQQLLITEFAELTALALANVRLRENLRYQSIRDPLTGLYNRRYLEDFL
FKQLHQAERTKASFAILMLDLDHFKKINDTFGHDAGDLVLKELGQILNSDIRLGDIAARYGGEEFVLLLYDIDAQAAKMK
AENLRSAISNLQVKYGAQPVGQITASIGISVYPDDAKSPAEVIEAADKALYQAKNKGRNKVILFSEISS

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=161, Percent_Identity=43.4782608695652, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1788381, Length=172, Percent_Identity=36.6279069767442, Blast_Score=113, Evalue=4e-26,
Organism=Escherichia coli, GI145693134, Length=169, Percent_Identity=34.9112426035503, Blast_Score=112, Evalue=1e-25,
Organism=Escherichia coli, GI1786584, Length=249, Percent_Identity=29.3172690763052, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI87081881, Length=170, Percent_Identity=37.0588235294118, Blast_Score=106, Evalue=6e-24,
Organism=Escherichia coli, GI87082007, Length=170, Percent_Identity=35.2941176470588, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1787816, Length=199, Percent_Identity=32.6633165829146, Blast_Score=97, Evalue=5e-21,
Organism=Escherichia coli, GI1788085, Length=165, Percent_Identity=35.1515151515151, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1787541, Length=173, Percent_Identity=31.2138728323699, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI87081974, Length=179, Percent_Identity=33.5195530726257, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1788956, Length=161, Percent_Identity=34.7826086956522, Blast_Score=84, Evalue=4e-17,
Organism=Escherichia coli, GI87081977, Length=224, Percent_Identity=31.6964285714286, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1787802, Length=164, Percent_Identity=30.4878048780488, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 80984; Mature: 80984

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSTWHQTAAGNRSILSNLRLSRTLLNVIFIIALSLLLIINIISYNQVKNLIFASNWVSH
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SNEVIQTIDTCLYDVVEIESRQRFYLIRGSSPQFMTDVDDMKSTLQLNLDKLEQLTKDNP
CHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHCCHHHHHHHHHCCHHHHHHHHCCCC
EQNKRTHRFIDLVKQRLNLLNQLVQLKGSGKLDTQEGYDLLNQSQDMSHQVKSLGQEIKS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHH
IELVLLSERNTGAVRWADTSSFILISGGITSILFLTIAFILANIELSTRRKTELQNQNTQ
EEEEEEECCCCCEEEEECCCCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCH
IRLKKIIESASDMIAAFDKDQRFITFNEAYQREFKRLFDKSISINMSLEEAFDDVPENKK
HHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHCCCCEEEEEHHHHHCCCCCHHH
KLVQTWKESLQRDEETKNIEVNTEQEKTIYEMTSKLIQNGDNEIKGVVHSVRNITKRVQE
HHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
HTELQESYEKLANGMKELQEKNEQITLLVEMSDIMLACGSQEELSDVMAKYSQRLLQFSS
HHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
GYLFIMHPSKNYLEKAASWGNPQPHDLTFTPEQCWAIRLGRIHYAGSSRIELMCSHTMFA
CEEEEECCCHHHHHHHHCCCCCCCCCCEECHHHHHHEEECEEEECCCCCEEEEEEHHHHC
EQPELSLLCVPLMAQNDIYGLLYLEVGLKFDENQQLLITEFAELTALALANVRLRENLRY
CCCCCEEEEEEHHCCCCCEEEEEEEECCEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCH
QSIRDPLTGLYNRRYLEDFLFKQLHQAERTKASFAILMLDLDHFKKINDTFGHDAGDLVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEHHHHHHHHHHCCCCHHHHHH
KELGQILNSDIRLGDIAARYGGEEFVLLLYDIDAQAAKMKAENLRSAISNLQVKYGAQPV
HHHHHHHCCCCCCHHHHHHCCCCEEEEEEEECCHHHHHHHHHHHHHHHHCCEECCCCCHH
GQITASIGISVYPDDAKSPAEVIEAADKALYQAKNKGRNKVILFSEISS
HHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
>Mature Secondary Structure
MNSTWHQTAAGNRSILSNLRLSRTLLNVIFIIALSLLLIINIISYNQVKNLIFASNWVSH
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SNEVIQTIDTCLYDVVEIESRQRFYLIRGSSPQFMTDVDDMKSTLQLNLDKLEQLTKDNP
CHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHCCHHHHHHHHHCCHHHHHHHHCCCC
EQNKRTHRFIDLVKQRLNLLNQLVQLKGSGKLDTQEGYDLLNQSQDMSHQVKSLGQEIKS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHH
IELVLLSERNTGAVRWADTSSFILISGGITSILFLTIAFILANIELSTRRKTELQNQNTQ
EEEEEEECCCCCEEEEECCCCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCH
IRLKKIIESASDMIAAFDKDQRFITFNEAYQREFKRLFDKSISINMSLEEAFDDVPENKK
HHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHCCCCEEEEEHHHHHCCCCCHHH
KLVQTWKESLQRDEETKNIEVNTEQEKTIYEMTSKLIQNGDNEIKGVVHSVRNITKRVQE
HHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
HTELQESYEKLANGMKELQEKNEQITLLVEMSDIMLACGSQEELSDVMAKYSQRLLQFSS
HHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
GYLFIMHPSKNYLEKAASWGNPQPHDLTFTPEQCWAIRLGRIHYAGSSRIELMCSHTMFA
CEEEEECCCHHHHHHHHCCCCCCCCCCEECHHHHHHEEECEEEECCCCCEEEEEEHHHHC
EQPELSLLCVPLMAQNDIYGLLYLEVGLKFDENQQLLITEFAELTALALANVRLRENLRY
CCCCCEEEEEEHHCCCCCEEEEEEEECCEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCH
QSIRDPLTGLYNRRYLEDFLFKQLHQAERTKASFAILMLDLDHFKKINDTFGHDAGDLVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEHHHHHHHHHHCCCCHHHHHH
KELGQILNSDIRLGDIAARYGGEEFVLLLYDIDAQAAKMKAENLRSAISNLQVKYGAQPV
HHHHHHHCCCCCCHHHHHHCCCCEEEEEEEECCHHHHHHHHHHHHHHHHCCEECCCCCHH
GQITASIGISVYPDDAKSPAEVIEAADKALYQAKNKGRNKVILFSEISS
HHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]