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The map label for this gene is artJ [H]

Identifier: 54293050

GI number: 54293050

Start: 101520

End: 102248

Strand: Reverse

Name: artJ [H]

Synonym: lpl0087

Alternate gene names: 54293050

Gene position: 102248-101520 (Counterclockwise)

Preceding gene: 54293051

Following gene: 54293049

Centisome position: 3.06

GC content: 38.55

Gene sequence:

>729_bases
ATGAAACGATTCATGTTCATCCTTTTGATCTTTATAAATTTCCACGCCTTTGGAAAAGCACTCATTATTGGCGTTCCAGA
TTTTGCTCCACCATTTGTATCAAGATCTGGTGGAGGTGAGTTTTATTTCGGATTCAACATTGATTTGATGAACTCGATTT
GCAAGATTATTGAGGAAGAATGCCAATACAAGGGCATGAACATTCAACAGCTATATACCAATCTCAACACAGGAAAGGTT
GATATTATGCTGGCACCTACTCCAATCGTATCCACTGCTGGCACCAATTATATCTTCAGTCTTCCTTATTTGCCGAGCAA
TGCCCAAATTGTAACCCTGAAAACTAATGACCATATCAATGCCTTAACGGATCTAAAAGGTAAAAAAGTTGGTACTTTAA
AATACACTTTATACGGTGGCCTCCTCCAGGAGCATTTTCAGGATTATGTTGATTTGATGCAATTTTCCAAACTTCCCGAT
ATGGCAATCGCATTGTCAAATCGACAAATCGAAGCAGTAGTGCTTAATGCCAATGCAGCGAAATACGCGATGAGCAATTC
CGCGACTGAGCTCAAATTTGTGGGAGATCCCATTCCTATAGGTAATGGCTATGGAATACTAGCCCTCAAAAATAATGCCC
CGCTCATCATGAAAATCAACAAGGCTTTATTACAAATAGAAAAAGACGGAACCTATTTGGAAATATATAATACCTATTTC
GGTGATTAA

Upstream 100 bases:

>100_bases
AAAACAAATAAGACTTCAATTAAAACACATATTGACTTTAATTTGACAAAATCGATTGCTCCTCCTAAATTTTAAGTACA
TCCTAAATGGACTTAAAAAC

Downstream 100 bases:

>100_bases
GATATTACCCTAAGCAATTCACCAGGTATTTCAAAAACCCGAAACTAGATCCAACTCCAGAATTCAGGTTAAACTAATAC
CAATTGAATCTGTCTCTCAT

Product: hypothetical protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MKRFMFILLIFINFHAFGKALIIGVPDFAPPFVSRSGGGEFYFGFNIDLMNSICKIIEEECQYKGMNIQQLYTNLNTGKV
DIMLAPTPIVSTAGTNYIFSLPYLPSNAQIVTLKTNDHINALTDLKGKKVGTLKYTLYGGLLQEHFQDYVDLMQFSKLPD
MAIALSNRQIEAVVLNANAAKYAMSNSATELKFVGDPIPIGNGYGILALKNNAPLIMKINKALLQIEKDGTYLEIYNTYF
GD

Sequences:

>Translated_242_residues
MKRFMFILLIFINFHAFGKALIIGVPDFAPPFVSRSGGGEFYFGFNIDLMNSICKIIEEECQYKGMNIQQLYTNLNTGKV
DIMLAPTPIVSTAGTNYIFSLPYLPSNAQIVTLKTNDHINALTDLKGKKVGTLKYTLYGGLLQEHFQDYVDLMQFSKLPD
MAIALSNRQIEAVVLNANAAKYAMSNSATELKFVGDPIPIGNGYGILALKNNAPLIMKINKALLQIEKDGTYLEIYNTYF
GD
>Mature_242_residues
MKRFMFILLIFINFHAFGKALIIGVPDFAPPFVSRSGGGEFYFGFNIDLMNSICKIIEEECQYKGMNIQQLYTNLNTGKV
DIMLAPTPIVSTAGTNYIFSLPYLPSNAQIVTLKTNDHINALTDLKGKKVGTLKYTLYGGLLQEHFQDYVDLMQFSKLPD
MAIALSNRQIEAVVLNANAAKYAMSNSATELKFVGDPIPIGNGYGILALKNNAPLIMKINKALLQIEKDGTYLEIYNTYF
GD

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=247, Percent_Identity=29.5546558704453, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI1788649, Length=254, Percent_Identity=24.0157480314961, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787088, Length=215, Percent_Identity=26.5116279069767, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788228, Length=222, Percent_Identity=26.1261261261261, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787031, Length=235, Percent_Identity=27.2340425531915, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788648, Length=225, Percent_Identity=25.7777777777778, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 26922; Mature: 26922

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRFMFILLIFINFHAFGKALIIGVPDFAPPFVSRSGGGEFYFGFNIDLMNSICKIIEEE
CHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHH
CQYKGMNIQQLYTNLNTGKVDIMLAPTPIVSTAGTNYIFSLPYLPSNAQIVTLKTNDHIN
HHHCCCCHHHHHHCCCCCEEEEEECCCCEEECCCCCEEEECCCCCCCCEEEEEECCCCCC
ALTDLKGKKVGTLKYTLYGGLLQEHFQDYVDLMQFSKLPDMAIALSNRQIEAVVLNANAA
HHHCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEECCCC
KYAMSNSATELKFVGDPIPIGNGYGILALKNNAPLIMKINKALLQIEKDGTYLEIYNTYF
EEEECCCCEEEEECCCCCCCCCCEEEEEECCCCCEEEEECCEEEEEECCCCEEEEEEECC
GD
CC
>Mature Secondary Structure
MKRFMFILLIFINFHAFGKALIIGVPDFAPPFVSRSGGGEFYFGFNIDLMNSICKIIEEE
CHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHH
CQYKGMNIQQLYTNLNTGKVDIMLAPTPIVSTAGTNYIFSLPYLPSNAQIVTLKTNDHIN
HHHCCCCHHHHHHCCCCCEEEEEECCCCEEECCCCCEEEECCCCCCCCEEEEEECCCCCC
ALTDLKGKKVGTLKYTLYGGLLQEHFQDYVDLMQFSKLPDMAIALSNRQIEAVVLNANAA
HHHCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEECCCC
KYAMSNSATELKFVGDPIPIGNGYGILALKNNAPLIMKINKALLQIEKDGTYLEIYNTYF
EEEECCCCEEEEECCCCCCCCCCEEEEEECCCCCEEEEECCEEEEEECCCCEEEEEEECC
GD
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]