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The map label for this gene is rlmL [H]

Identifier: 54292987

GI number: 54292987

Start: 28123

End: 30246

Strand: Direct

Name: rlmL [H]

Synonym: lpl0023

Alternate gene names: 54292987

Gene position: 28123-30246 (Clockwise)

Preceding gene: 54292986

Following gene: 54292991

Centisome position: 0.84

GC content: 39.36

Gene sequence:

>2124_bases
ATGAATTACTCTCTATTCATTAGTTGTGCAAAAGGTTTGGAGTATTTGCTGGAAGATGAACTCAAGGGATTGGGGCTTCA
TGTGACTCAAGTGAGTCCTCAAGGTGTCTATGGTGAGGCTAGCCTGCCTGTAATTTATAATTTATGTTTATGGTCAAGGC
TTGCTAACAGAATACAGCTTATTTTATTTAGTGGCCATGCTGCAAAAGAACAAGCCGTACATCAGTTATGCACAGACTTT
CATTGGCAAACGGTTTTCACCCATGATAAAACCATAGCCATCGAATTTCATGGTGCATCAGAGCAAATTCGTAATACCAT
GTTTGGCGCTCAGATAGTAAAAGATGGCATAGTGGATCATTTTCGACGCTTGAATGGCTCAAGGCCTTCTGTCGACAAAG
AAAAACCGCAAATTTTAATTCATGCTCATTTGAAAAATGATATTTTAACGGTGAGCTTTGATTTGGTTGGATATAGCCTG
CATCAAAGAGGATATCGTAAAAAAGCAGGGAAGGCTCCTTTAAAGGAAAACGTTGCAGCAGCGATGTTATTAAGGGCCAA
ATGGCCAGAGTTGGCCGCACAGGGATATGGCTTACATGATCCGTTTTGCGGTTCAGGCACATTAGTTATCGAAGCAGCCA
TGATGGCAGCTCATATTGCTCCAGGCTTATTGCGGCAAGATCAATCCTTACAATATTGGGCTCGACATCAGAGTTCTTTA
TGGGAAAAATTACGAACTCAGGCATTACAGCAAGTGAAGCCGTTGGCAGTTAAGCTGGTTGGTACGGACACGGACAGCAA
AGTAGTTGCTCTTGCGCGTTCCAATGCTGAGAGAGCAGGCGTATTACCTTTGGTTGAATTTAATACATTATCGCTAAATG
CTTGTAGGCCAGGGACAAAAAGAGGATTAGTTGTTTGTAATCCACCTTACGGTGAGCGATTGGGGGAGGTAACTCAACTT
GTTCCTTTATATCAACAATTGGGTACTACATTACATACTTGTTATCAAGGTTGGCAAGCGGCTATTTTAACTTCCAGTCC
GGTTTTGGCTAAAGCATTAGGATTACGTGCTGATAAGCAATATACTCTATATAATGGCCCATTAGAGTGCAAACTCTATT
GTTTGACTCTTTCTGCTGCAAATAAACTAAAAAACACCCCAAATGCCCCGCTTTCTGATAATGCACAAATGCTATTTAAC
CGATTGGAAAAAAACAGAAATCATTTACAGAAATGGGCAAGAAAAAATCAAATCACCTGTTATCGAATTTACGATGCGGA
TTTACCCGAATATGCCTATGCCATAGACATATATAATGACTATGCTGTACTGCAAGAGTATGCGCCACCAGCCAGCATTC
CAGTACACAAGGCAGAAAAACGCAGCCTTGAGATGTTGCAAGTCGTTCCACGGGCTTTAGGTATTCACCCTGAAAAATTA
ATAGTTAAACAAAGAAAGCAGCAAAAGGGTTCAGAACAATATCAAAAAATAGGTAAAACCAGCCAAAGACAGATAGTGAC
AGAAGGTAAGGCAAAATTGATAGTGAATTTGTATGATTATCTTGATACAGGACTATTTCTGGATCATAGACTTATGCGCT
TGAAATTTGCTCAATTAGAGCAGGGAACTCGATTTTTGAACTGTTTTTGTTATACTGCCAGTGCAAGCGTCCATGCTGCC
TTGGCCGGTGCATTAACCACGAATGTTGATTTATCCAAAACGTATTTGCTTTGGGCGGAAGATAATTTTAGATTAAATGA
TATTAATTTATCCAAGCATCAATTTTTGCAATACGATTGTAAGGAATGGATGAAAACTACCAGGGATAAATTTGATGTTA
TTTTTTTGGATCCCCCCAGCTTTTCAAACTCAAAGCGTATGAGCGATATTTTAGACATTCAACGAGATCATGTTTCTTTG
ATTAACATGGCAATGCGGTTGTTAAATCCTGATGGTGTTTTATATTTCTCAACTAATCTGCGCCAATTTAAGTTAGAGCC
AATGCTAAAAGAGAAATATGCAGTACAAGACATTACACCACAGACCATCGATCAGGATTTTAAACGAAATAGCAAAATTC
ACCATTGTTTTAAAATTGTAATGCCGCATTTTGCGGATAACTGA

Upstream 100 bases:

>100_bases
AATTAAGGCAACTGATTAAAAAGGCATTAGATGATCAGATGAAAGAGAAAAATACGGGTGCTGCTAAAGCACTGTTTCGC
TATTTAAGGTCTTTAATAAA

Downstream 100 bases:

>100_bases
ATAATTCAGTTATTTATTATTAGATCTCTTGCCGAGCTTCCCTTTCATAAGCGTGAAGCTCGACTCTCTCTAAGATTTGG
ATTTTGTTTTGGTTTTGCTT

Product: 23S rRNA m(2)G2445 methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 707; Mature: 707

Protein sequence:

>707_residues
MNYSLFISCAKGLEYLLEDELKGLGLHVTQVSPQGVYGEASLPVIYNLCLWSRLANRIQLILFSGHAAKEQAVHQLCTDF
HWQTVFTHDKTIAIEFHGASEQIRNTMFGAQIVKDGIVDHFRRLNGSRPSVDKEKPQILIHAHLKNDILTVSFDLVGYSL
HQRGYRKKAGKAPLKENVAAAMLLRAKWPELAAQGYGLHDPFCGSGTLVIEAAMMAAHIAPGLLRQDQSLQYWARHQSSL
WEKLRTQALQQVKPLAVKLVGTDTDSKVVALARSNAERAGVLPLVEFNTLSLNACRPGTKRGLVVCNPPYGERLGEVTQL
VPLYQQLGTTLHTCYQGWQAAILTSSPVLAKALGLRADKQYTLYNGPLECKLYCLTLSAANKLKNTPNAPLSDNAQMLFN
RLEKNRNHLQKWARKNQITCYRIYDADLPEYAYAIDIYNDYAVLQEYAPPASIPVHKAEKRSLEMLQVVPRALGIHPEKL
IVKQRKQQKGSEQYQKIGKTSQRQIVTEGKAKLIVNLYDYLDTGLFLDHRLMRLKFAQLEQGTRFLNCFCYTASASVHAA
LAGALTTNVDLSKTYLLWAEDNFRLNDINLSKHQFLQYDCKEWMKTTRDKFDVIFLDPPSFSNSKRMSDILDIQRDHVSL
INMAMRLLNPDGVLYFSTNLRQFKLEPMLKEKYAVQDITPQTIDQDFKRNSKIHHCFKIVMPHFADN

Sequences:

>Translated_707_residues
MNYSLFISCAKGLEYLLEDELKGLGLHVTQVSPQGVYGEASLPVIYNLCLWSRLANRIQLILFSGHAAKEQAVHQLCTDF
HWQTVFTHDKTIAIEFHGASEQIRNTMFGAQIVKDGIVDHFRRLNGSRPSVDKEKPQILIHAHLKNDILTVSFDLVGYSL
HQRGYRKKAGKAPLKENVAAAMLLRAKWPELAAQGYGLHDPFCGSGTLVIEAAMMAAHIAPGLLRQDQSLQYWARHQSSL
WEKLRTQALQQVKPLAVKLVGTDTDSKVVALARSNAERAGVLPLVEFNTLSLNACRPGTKRGLVVCNPPYGERLGEVTQL
VPLYQQLGTTLHTCYQGWQAAILTSSPVLAKALGLRADKQYTLYNGPLECKLYCLTLSAANKLKNTPNAPLSDNAQMLFN
RLEKNRNHLQKWARKNQITCYRIYDADLPEYAYAIDIYNDYAVLQEYAPPASIPVHKAEKRSLEMLQVVPRALGIHPEKL
IVKQRKQQKGSEQYQKIGKTSQRQIVTEGKAKLIVNLYDYLDTGLFLDHRLMRLKFAQLEQGTRFLNCFCYTASASVHAA
LAGALTTNVDLSKTYLLWAEDNFRLNDINLSKHQFLQYDCKEWMKTTRDKFDVIFLDPPSFSNSKRMSDILDIQRDHVSL
INMAMRLLNPDGVLYFSTNLRQFKLEPMLKEKYAVQDITPQTIDQDFKRNSKIHHCFKIVMPHFADN
>Mature_707_residues
MNYSLFISCAKGLEYLLEDELKGLGLHVTQVSPQGVYGEASLPVIYNLCLWSRLANRIQLILFSGHAAKEQAVHQLCTDF
HWQTVFTHDKTIAIEFHGASEQIRNTMFGAQIVKDGIVDHFRRLNGSRPSVDKEKPQILIHAHLKNDILTVSFDLVGYSL
HQRGYRKKAGKAPLKENVAAAMLLRAKWPELAAQGYGLHDPFCGSGTLVIEAAMMAAHIAPGLLRQDQSLQYWARHQSSL
WEKLRTQALQQVKPLAVKLVGTDTDSKVVALARSNAERAGVLPLVEFNTLSLNACRPGTKRGLVVCNPPYGERLGEVTQL
VPLYQQLGTTLHTCYQGWQAAILTSSPVLAKALGLRADKQYTLYNGPLECKLYCLTLSAANKLKNTPNAPLSDNAQMLFN
RLEKNRNHLQKWARKNQITCYRIYDADLPEYAYAIDIYNDYAVLQEYAPPASIPVHKAEKRSLEMLQVVPRALGIHPEKL
IVKQRKQQKGSEQYQKIGKTSQRQIVTEGKAKLIVNLYDYLDTGLFLDHRLMRLKFAQLEQGTRFLNCFCYTASASVHAA
LAGALTTNVDLSKTYLLWAEDNFRLNDINLSKHQFLQYDCKEWMKTTRDKFDVIFLDPPSFSNSKRMSDILDIQRDHVSL
INMAMRLLNPDGVLYFSTNLRQFKLEPMLKEKYAVQDITPQTIDQDFKRNSKIHHCFKIVMPHFADN

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=709, Percent_Identity=43.441466854725, Blast_Score=572, Evalue=1e-164,
Organism=Escherichia coli, GI145693111, Length=257, Percent_Identity=29.5719844357977, Blast_Score=91, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 80205; Mature: 80205

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE ; PS01261 UPF0020

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYSLFISCAKGLEYLLEDELKGLGLHVTQVSPQGVYGEASLPVIYNLCLWSRLANRIQL
CCEEEEEEHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHEEE
ILFSGHAAKEQAVHQLCTDFHWQTVFTHDKTIAIEFHGASEQIRNTMFGAQIVKDGIVDH
EEECCCCHHHHHHHHHHHHCCCEEEEECCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
FRRLNGSRPSVDKEKPQILIHAHLKNDILTVSFDLVGYSLHQRGYRKKAGKAPLKENVAA
HHHHCCCCCCCCCCCCEEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHH
AMLLRAKWPELAAQGYGLHDPFCGSGTLVIEAAMMAAHIAPGLLRQDQSLQYWARHQSSL
HHHHHHCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHH
WEKLRTQALQQVKPLAVKLVGTDTDSKVVALARSNAERAGVLPLVEFNTLSLNACRPGTK
HHHHHHHHHHHCCCEEEEEEECCCCCEEEEEECCCCCCCCCCEEEEECEEEEECCCCCCC
RGLVVCNPPYGERLGEVTQLVPLYQQLGTTLHTCYQGWQAAILTSSPVLAKALGLRADKQ
CCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEEEECCCHHHHHHCCCCCCC
YTLYNGPLECKLYCLTLSAANKLKNTPNAPLSDNAQMLFNRLEKNRNHLQKWARKNQITC
EEEECCCCEEEEEEEEEHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEE
YRIYDADLPEYAYAIDIYNDYAVLQEYAPPASIPVHKAEKRSLEMLQVVPRALGIHPEKL
EEEECCCCCCEEEEEEECCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHH
IVKQRKQQKGSEQYQKIGKTSQRQIVTEGKAKLIVNLYDYLDTGLFLDHRLMRLKFAQLE
HHHHHHHHCCHHHHHHHCCCHHHHHHHCCCCEEEEEHHHHHCCCHHHHHHHHHHHHHHHH
QGTRFLNCFCYTASASVHAALAGALTTNVDLSKTYLLWAEDNFRLNDINLSKHQFLQYDC
CCCEEEEEEEEECCCHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEECCCCHHHHHHHHH
KEWMKTTRDKFDVIFLDPPSFSNSKRMSDILDIQRDHVSLINMAMRLLNPDGVLYFSTNL
HHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCC
RQFKLEPMLKEKYAVQDITPQTIDQDFKRNSKIHHCFKIVMPHFADN
EEEECCHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNYSLFISCAKGLEYLLEDELKGLGLHVTQVSPQGVYGEASLPVIYNLCLWSRLANRIQL
CCEEEEEEHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHEEE
ILFSGHAAKEQAVHQLCTDFHWQTVFTHDKTIAIEFHGASEQIRNTMFGAQIVKDGIVDH
EEECCCCHHHHHHHHHHHHCCCEEEEECCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
FRRLNGSRPSVDKEKPQILIHAHLKNDILTVSFDLVGYSLHQRGYRKKAGKAPLKENVAA
HHHHCCCCCCCCCCCCEEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHH
AMLLRAKWPELAAQGYGLHDPFCGSGTLVIEAAMMAAHIAPGLLRQDQSLQYWARHQSSL
HHHHHHCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHH
WEKLRTQALQQVKPLAVKLVGTDTDSKVVALARSNAERAGVLPLVEFNTLSLNACRPGTK
HHHHHHHHHHHCCCEEEEEEECCCCCEEEEEECCCCCCCCCCEEEEECEEEEECCCCCCC
RGLVVCNPPYGERLGEVTQLVPLYQQLGTTLHTCYQGWQAAILTSSPVLAKALGLRADKQ
CCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEEEECCCHHHHHHCCCCCCC
YTLYNGPLECKLYCLTLSAANKLKNTPNAPLSDNAQMLFNRLEKNRNHLQKWARKNQITC
EEEECCCCEEEEEEEEEHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEE
YRIYDADLPEYAYAIDIYNDYAVLQEYAPPASIPVHKAEKRSLEMLQVVPRALGIHPEKL
EEEECCCCCCEEEEEEECCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHH
IVKQRKQQKGSEQYQKIGKTSQRQIVTEGKAKLIVNLYDYLDTGLFLDHRLMRLKFAQLE
HHHHHHHHCCHHHHHHHCCCHHHHHHHCCCCEEEEEHHHHHCCCHHHHHHHHHHHHHHHH
QGTRFLNCFCYTASASVHAALAGALTTNVDLSKTYLLWAEDNFRLNDINLSKHQFLQYDC
CCCEEEEEEEEECCCHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEECCCCHHHHHHHHH
KEWMKTTRDKFDVIFLDPPSFSNSKRMSDILDIQRDHVSLINMAMRLLNPDGVLYFSTNL
HHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCC
RQFKLEPMLKEKYAVQDITPQTIDQDFKRNSKIHHCFKIVMPHFADN
EEEECCHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA