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The map label for this gene is pilR [H]

Identifier: 54297131

GI number: 54297131

Start: 1310755

End: 1312083

Strand: Direct

Name: pilR [H]

Synonym: lpp1176

Alternate gene names: 54297131

Gene position: 1310755-1312083 (Clockwise)

Preceding gene: 54297130

Following gene: 54297132

Centisome position: 37.41

GC content: 40.48

Gene sequence:

>1329_bases
ATGACCAAAAGTAAAGTGCTTGTTATAGATGACGAACCAGATATAAGAGAGTTATTAACTCTTACTTTATCACGTATGGG
TTTAACTTGCGATGCAGTGTCTGACTTTAAGCAAGGCATAGAACATATAAAACAAAATTATTATTCTTTGGTTTTAACCG
ATATGCGTTTACCTGATGGAGATGGTATAGAAATTGTTAAATTCATTCAGAAATACAAACCGCAGATGCCAGTTGCCGTC
ATTACTGCCTATGGAAATGTCGAGGGTGCGGTCAACACTTTAAAGGCGGGTGCATTTGATTACGTATCAAAACCTATAGA
CTTAAAAATGCTTAGAGATCTCGTTAAGACGGCATTATCCATGCAAAACCCGCCTACCGAGAAGCCTGATGAAGATCTAC
TGGTTGGTGAAAGTTTGGTAATGCAACAACTTCGTGAAAACATTCATAAATTAGCTCGCAGTCAGGCACCAGTTTTTATT
CAAGGAGCTTCAGGTGTTGGGAAAGAATTGGTCGCTCAGTTGATCCATGTTAATGGTCCCAGAAAGGATAAGCCATTTAT
TCCTGTAAACTGTGGCGCAATACCTGGTGAGTTGATGGAATCAGAGTTTTTTGGCCATAAAAAAGGGAGCTTTACAGGAG
CGATTTCTGATAAAACAGGGTTGTTTGTTGCGGCTCATGGTGGCACTTTGTTTCTTGATGAAATCGCCGAACTTCCTCTC
GCCATGCAGGTAAAATTGCTACGTGCCATTCAGGAGAAGGCAATTAAGCCGATAGGTGAGTTATATGAAGTTCCTGTGGA
TGTTCGCATCCTAAGTGCGAGTCATAAAAATTTGATTGAGGAAGTTAATGCAGGGGCATTTCGTCAAGATTTGTATTATC
GAATTAATGTGATTGAATTGCGTGTCCCAACTTTAATGGAAAGGCTTTCAGATATTCCTTGCCTGACAGAGGCGATATTA
AGAAAATTATCTAAAAATCAAAACAGAAACCTGGTGAAAATAAATCCATCCTGTTTTGAAATATTGAGTCGTTATCATTT
TCCAGGAAATGTCAGAGAACTGGAGAATATCCTCGAGCGTGCCATGGCTATGTGTGAAGGAGAGGTTATAGAACCATCCG
ATTTGCATTTGCCCAGAACAACAATAACCCCAGCTGTTGCCGTACCCTCCACATCAACTAACTTAAATGGTTACCTTCAG
GATCAGGAAAAAGAGTTAATTCTTGATGCGCTCGAGAAAACAAAATGGAACAGAACCGCTGCTGCCCAACTACTGGGGGT
CAGCTTCCGAACTCTCCGTTATCGATTAAAAAAACTGGGCCTTGATTAG

Upstream 100 bases:

>100_bases
GAGATTTATGTGAAATTAATCAAGCCAGATTAAATTTGGTAAAATCAAATAAAGGATGTTGTTTTGCGATCACTGTTAAT
CCATCGGATGAACTTTTAAT

Downstream 100 bases:

>100_bases
ATGTTGCGCTTTGAAGATTTTCAAAGGTTAAAATAGACCGCTTGCCTAGCCAGCCTTTTTCGTTTTATTGTAGCCCTGAA
AACGTTTCTGAGGTGCTCAT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MTKSKVLVIDDEPDIRELLTLTLSRMGLTCDAVSDFKQGIEHIKQNYYSLVLTDMRLPDGDGIEIVKFIQKYKPQMPVAV
ITAYGNVEGAVNTLKAGAFDYVSKPIDLKMLRDLVKTALSMQNPPTEKPDEDLLVGESLVMQQLRENIHKLARSQAPVFI
QGASGVGKELVAQLIHVNGPRKDKPFIPVNCGAIPGELMESEFFGHKKGSFTGAISDKTGLFVAAHGGTLFLDEIAELPL
AMQVKLLRAIQEKAIKPIGELYEVPVDVRILSASHKNLIEEVNAGAFRQDLYYRINVIELRVPTLMERLSDIPCLTEAIL
RKLSKNQNRNLVKINPSCFEILSRYHFPGNVRELENILERAMAMCEGEVIEPSDLHLPRTTITPAVAVPSTSTNLNGYLQ
DQEKELILDALEKTKWNRTAAAQLLGVSFRTLRYRLKKLGLD

Sequences:

>Translated_442_residues
MTKSKVLVIDDEPDIRELLTLTLSRMGLTCDAVSDFKQGIEHIKQNYYSLVLTDMRLPDGDGIEIVKFIQKYKPQMPVAV
ITAYGNVEGAVNTLKAGAFDYVSKPIDLKMLRDLVKTALSMQNPPTEKPDEDLLVGESLVMQQLRENIHKLARSQAPVFI
QGASGVGKELVAQLIHVNGPRKDKPFIPVNCGAIPGELMESEFFGHKKGSFTGAISDKTGLFVAAHGGTLFLDEIAELPL
AMQVKLLRAIQEKAIKPIGELYEVPVDVRILSASHKNLIEEVNAGAFRQDLYYRINVIELRVPTLMERLSDIPCLTEAIL
RKLSKNQNRNLVKINPSCFEILSRYHFPGNVRELENILERAMAMCEGEVIEPSDLHLPRTTITPAVAVPSTSTNLNGYLQ
DQEKELILDALEKTKWNRTAAAQLLGVSFRTLRYRLKKLGLD
>Mature_441_residues
TKSKVLVIDDEPDIRELLTLTLSRMGLTCDAVSDFKQGIEHIKQNYYSLVLTDMRLPDGDGIEIVKFIQKYKPQMPVAVI
TAYGNVEGAVNTLKAGAFDYVSKPIDLKMLRDLVKTALSMQNPPTEKPDEDLLVGESLVMQQLRENIHKLARSQAPVFIQ
GASGVGKELVAQLIHVNGPRKDKPFIPVNCGAIPGELMESEFFGHKKGSFTGAISDKTGLFVAAHGGTLFLDEIAELPLA
MQVKLLRAIQEKAIKPIGELYEVPVDVRILSASHKNLIEEVNAGAFRQDLYYRINVIELRVPTLMERLSDIPCLTEAILR
KLSKNQNRNLVKINPSCFEILSRYHFPGNVRELENILERAMAMCEGEVIEPSDLHLPRTTITPAVAVPSTSTNLNGYLQD
QEKELILDALEKTKWNRTAAAQLLGVSFRTLRYRLKKLGLD

Specific function: Member of the two-component regulatory system pilS/pilR that regulates the expression of type 4 fimbriae. When activated by pilS it acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=438, Percent_Identity=41.324200913242, Blast_Score=336, Evalue=2e-93,
Organism=Escherichia coli, GI1788550, Length=453, Percent_Identity=38.6313465783664, Blast_Score=332, Evalue=2e-92,
Organism=Escherichia coli, GI1788905, Length=433, Percent_Identity=37.8752886836028, Blast_Score=305, Evalue=6e-84,
Organism=Escherichia coli, GI1790299, Length=470, Percent_Identity=36.1702127659575, Blast_Score=294, Evalue=1e-80,
Organism=Escherichia coli, GI1789233, Length=336, Percent_Identity=41.3690476190476, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI87082117, Length=321, Percent_Identity=38.3177570093458, Blast_Score=224, Evalue=8e-60,
Organism=Escherichia coli, GI87082152, Length=325, Percent_Identity=40.3076923076923, Blast_Score=221, Evalue=7e-59,
Organism=Escherichia coli, GI1786524, Length=319, Percent_Identity=41.0658307210031, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI1789087, Length=314, Percent_Identity=38.2165605095541, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI1787583, Length=334, Percent_Identity=35.9281437125749, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI87081872, Length=318, Percent_Identity=35.5345911949685, Blast_Score=184, Evalue=8e-48,
Organism=Escherichia coli, GI87081858, Length=305, Percent_Identity=32.4590163934426, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1789828, Length=259, Percent_Identity=32.8185328185328, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1786599, Length=122, Percent_Identity=30.327868852459, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790552, Length=132, Percent_Identity=30.3030303030303, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87082012, Length=165, Percent_Identity=30.9090909090909, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1786911, Length=127, Percent_Identity=30.7086614173228, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786784, Length=115, Percent_Identity=33.0434782608696, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1789809, Length=118, Percent_Identity=28.8135593220339, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49299; Mature: 49168

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSKVLVIDDEPDIRELLTLTLSRMGLTCDAVSDFKQGIEHIKQNYYSLVLTDMRLPDG
CCCCCEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCC
DGIEIVKFIQKYKPQMPVAVITAYGNVEGAVNTLKAGAFDYVSKPIDLKMLRDLVKTALS
CCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHH
MQNPPTEKPDEDLLVGESLVMQQLRENIHKLARSQAPVFIQGASGVGKELVAQLIHVNGP
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCC
RKDKPFIPVNCGAIPGELMESEFFGHKKGSFTGAISDKTGLFVAAHGGTLFLDEIAELPL
CCCCCEEEEECCCCCHHHHHHHHCCCCCCCEEEEECCCCCEEEEECCCEEEHHHHHHCCH
AMQVKLLRAIQEKAIKPIGELYEVPVDVRILSASHKNLIEEVNAGAFRQDLYYRINVIEL
HHHHHHHHHHHHHHHCHHHHHHHCCCCEEEECCHHHHHHHHHCCCHHHHHHEEEEEEEEE
RVPTLMERLSDIPCLTEAILRKLSKNQNRNLVKINPSCFEILSRYHFPGNVRELENILER
EHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHCCCCCHHHHHHHHHH
AMAMCEGEVIEPSDLHLPRTTITPAVAVPSTSTNLNGYLQDQEKELILDALEKTKWNRTA
HHHHHCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCEECCHHHHHHHHHHHHHCCCHHH
AAQLLGVSFRTLRYRLKKLGLD
HHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKSKVLVIDDEPDIRELLTLTLSRMGLTCDAVSDFKQGIEHIKQNYYSLVLTDMRLPDG
CCCCEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCC
DGIEIVKFIQKYKPQMPVAVITAYGNVEGAVNTLKAGAFDYVSKPIDLKMLRDLVKTALS
CCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHH
MQNPPTEKPDEDLLVGESLVMQQLRENIHKLARSQAPVFIQGASGVGKELVAQLIHVNGP
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCC
RKDKPFIPVNCGAIPGELMESEFFGHKKGSFTGAISDKTGLFVAAHGGTLFLDEIAELPL
CCCCCEEEEECCCCCHHHHHHHHCCCCCCCEEEEECCCCCEEEEECCCEEEHHHHHHCCH
AMQVKLLRAIQEKAIKPIGELYEVPVDVRILSASHKNLIEEVNAGAFRQDLYYRINVIEL
HHHHHHHHHHHHHHHCHHHHHHHCCCCEEEECCHHHHHHHHHCCCHHHHHHEEEEEEEEE
RVPTLMERLSDIPCLTEAILRKLSKNQNRNLVKINPSCFEILSRYHFPGNVRELENILER
EHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHCCCCCHHHHHHHHHH
AMAMCEGEVIEPSDLHLPRTTITPAVAVPSTSTNLNGYLQDQEKELILDALEKTKWNRTA
HHHHHCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCEECCHHHHHHHHHHHHHCCCHHH
AAQLLGVSFRTLRYRLKKLGLD
HHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1317379; 8097014; 7911557; 10984043 [H]