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The map label for this gene is haeIIM [H]

Identifier: 54297036

GI number: 54297036

Start: 1195761

End: 1196723

Strand: Direct

Name: haeIIM [H]

Synonym: lpp1078

Alternate gene names: 54297036

Gene position: 1195761-1196723 (Clockwise)

Preceding gene: 54297035

Following gene: 54297044

Centisome position: 34.13

GC content: 33.23

Gene sequence:

>963_bases
GTGCGTAGGAAAGCATTTAAATTCATAGACTTATTTGCTGGAATTGGTGGTATTCGAATTCCATTTGATAAATTAAATGG
CGAATGTGTTTTCTCTTCCGAAATAGATCCATATTGTCAAAAGACATATGAAGCTAATTTTGGTGAGAAACCTTTTGGAG
ATATAACAAAGATATCGCCGCAAGATGTACCTGATCACGATATTTTACTTGGTGGTTTTCCCTGCCAAGCATTTTCCATA
ATTGGTAGACAAAAAGGTTTTTCTGATACAAGAGGAACTCTTTTCTTTAATATTGAACAAATCTTGAAAGTAAAACGCCC
TAGAGCTTTTTTGCTGGAAAATGTGAAGCAACTGTCTACACATGATGGAGGTCGTACTTTTCAAATAATTATTGAAACAC
TTAGAAACTTGGGATATACAGTCTATAGTACCATTTTAAATTCCTTGGATTTTGGAGTTCCCCAAAAAAGAGAGCGAACA
TATATTGTTGGATTTATTGATAATGTTCCGTTCGAATTTCCTAAAGGCGGGGTTCCATTTAATTTGGAATCCATACTTGA
AAAAGAGCAGCAAATACCGAATAAATATTATGCTTCAGATTATATTAGACAGAAAAGAATTGCGGCATTGAAAGGTGTCC
CCCCTATACCAAGTATTTGGCATGAAAATAAATCGGGAAATATTTCAACACTTCCTTATTCATGTGCTTTAAGAGCGACA
GCATCATATAGTTATTTGTTAGTTAATGGGGAAAGAAGATTAACTGAAAGAGAAATGCTTCGATTACAAGGATTTCCTGA
TACTTTTAAAATAGTTTGTAATTATACTCAAACTAGGAAGCAAGCTGGCAATGCTGTAACAGTTAATGTAATAGATGCAA
TTGCTAAAAAATTGATTGATTCGTTAGAAATGGCAAATGATATGAATAAAATTAAGTTTCATAAAACATTTGCTACAGCT
TGA

Upstream 100 bases:

>100_bases
GGATAACTCTGATAAAGAAAAGTAATAAAAAAATAAAATTGTTCCTAGCAACTCTAACAATAACTATAATATGGAATTAC
TTTCTCGGGAGAATAGCATC

Downstream 100 bases:

>100_bases
TTTTTATTCTCTGGGAACAATACGATAGGATTGTTCATTAAAGCCGATAGTCTCTAGGATTGACTGAGTTGAGATTGTTT
TAGGAGGGACAATTTCAATA

Product: hypothetical protein

Products: NA

Alternate protein names: M.HaeII; Cytosine-specific methyltransferase HaeII [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MRRKAFKFIDLFAGIGGIRIPFDKLNGECVFSSEIDPYCQKTYEANFGEKPFGDITKISPQDVPDHDILLGGFPCQAFSI
IGRQKGFSDTRGTLFFNIEQILKVKRPRAFLLENVKQLSTHDGGRTFQIIIETLRNLGYTVYSTILNSLDFGVPQKRERT
YIVGFIDNVPFEFPKGGVPFNLESILEKEQQIPNKYYASDYIRQKRIAALKGVPPIPSIWHENKSGNISTLPYSCALRAT
ASYSYLLVNGERRLTEREMLRLQGFPDTFKIVCNYTQTRKQAGNAVTVNVIDAIAKKLIDSLEMANDMNKIKFHKTFATA

Sequences:

>Translated_320_residues
MRRKAFKFIDLFAGIGGIRIPFDKLNGECVFSSEIDPYCQKTYEANFGEKPFGDITKISPQDVPDHDILLGGFPCQAFSI
IGRQKGFSDTRGTLFFNIEQILKVKRPRAFLLENVKQLSTHDGGRTFQIIIETLRNLGYTVYSTILNSLDFGVPQKRERT
YIVGFIDNVPFEFPKGGVPFNLESILEKEQQIPNKYYASDYIRQKRIAALKGVPPIPSIWHENKSGNISTLPYSCALRAT
ASYSYLLVNGERRLTEREMLRLQGFPDTFKIVCNYTQTRKQAGNAVTVNVIDAIAKKLIDSLEMANDMNKIKFHKTFATA
>Mature_320_residues
MRRKAFKFIDLFAGIGGIRIPFDKLNGECVFSSEIDPYCQKTYEANFGEKPFGDITKISPQDVPDHDILLGGFPCQAFSI
IGRQKGFSDTRGTLFFNIEQILKVKRPRAFLLENVKQLSTHDGGRTFQIIIETLRNLGYTVYSTILNSLDFGVPQKRERT
YIVGFIDNVPFEFPKGGVPFNLESILEKEQQIPNKYYASDYIRQKRIAALKGVPPIPSIWHENKSGNISTLPYSCALRAT
ASYSYLLVNGERRLTEREMLRLQGFPDTFKIVCNYTQTRKQAGNAVTVNVIDAIAKKLIDSLEMANDMNKIKFHKTFATA

Specific function: This methylase recognizes the double-stranded sequence RGCGCY, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the HaeII endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4758184, Length=183, Percent_Identity=26.775956284153, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1788271, Length=352, Percent_Identity=33.2386363636364, Blast_Score=152, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 36305; Mature: 36305

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRKAFKFIDLFAGIGGIRIPFDKLNGECVFSSEIDPYCQKTYEANFGEKPFGDITKISP
CCCHHHHHHHHHHCCCCCCCCHHHCCCCEEECCCCCHHHHHHHCCCCCCCCCCCCCCCCC
QDVPDHDILLGGFPCQAFSIIGRQKGFSDTRGTLFFNIEQILKVKRPRAFLLENVKQLST
CCCCCCCEEEECCCHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHCCCHHHHHHHHHHHHC
HDGGRTFQIIIETLRNLGYTVYSTILNSLDFGVPQKRERTYIVGFIDNVPFEFPKGGVPF
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCC
NLESILEKEQQIPNKYYASDYIRQKRIAALKGVPPIPSIWHENKSGNISTLPYSCALRAT
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCEEECCHHHEEEEE
ASYSYLLVNGERRLTEREMLRLQGFPDTFKIVCNYTQTRKQAGNAVTVNVIDAIAKKLID
CCEEEEEECCCHHHHHHHHHHHCCCCCHHHHEECCHHHHHHCCCEEEEHHHHHHHHHHHH
SLEMANDMNKIKFHKTFATA
HHHHHCCHHHHEEEHHHCCC
>Mature Secondary Structure
MRRKAFKFIDLFAGIGGIRIPFDKLNGECVFSSEIDPYCQKTYEANFGEKPFGDITKISP
CCCHHHHHHHHHHCCCCCCCCHHHCCCCEEECCCCCHHHHHHHCCCCCCCCCCCCCCCCC
QDVPDHDILLGGFPCQAFSIIGRQKGFSDTRGTLFFNIEQILKVKRPRAFLLENVKQLST
CCCCCCCEEEECCCHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHCCCHHHHHHHHHHHHC
HDGGRTFQIIIETLRNLGYTVYSTILNSLDFGVPQKRERTYIVGFIDNVPFEFPKGGVPF
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCC
NLESILEKEQQIPNKYYASDYIRQKRIAALKGVPPIPSIWHENKSGNISTLPYSCALRAT
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCEEECCHHHEEEEE
ASYSYLLVNGERRLTEREMLRLQGFPDTFKIVCNYTQTRKQAGNAVTVNVIDAIAKKLID
CCEEEEEECCCHHHHHHHHHHHCCCCCHHHHEECCHHHHHHCCCEEEEHHHHHHHHHHHH
SLEMANDMNKIKFHKTFATA
HHHHHCCHHHHEEEHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9628358 [H]