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The map label for this gene is pckA

Identifier: 54027453

GI number: 54027453

Start: 5803395

End: 5805227

Strand: Reverse

Name: pckA

Synonym: nfa54790

Alternate gene names: 54027453

Gene position: 5805227-5803395 (Counterclockwise)

Preceding gene: 54027454

Following gene: 54027452

Centisome position: 96.41

GC content: 67.65

Gene sequence:

>1833_bases
ATGACCTCAGCGACCATTCCTGGTCTTCGTGGATCCGACGGCCAGGCGCCGACCGAGCACAGCGAACTACTCGCCTGGGT
ACAGGAAGTCGCAGAACTCACCCAGCCGGAGCGGGTGGTCTGGGCGGACGGTTCCGACGAGGAGTGGGAGCGGCTGACCG
ATCAGCTGGTGGAGGCGGGCACCTTCAAGCGCCTCGACCCGAAGAAGAAGCCGAACTCGTTCCTGGCGCTCTCCGACCCG
TCCGACGTGGCCCGGGTGGAGTCGCGCACCTTCATCTGCTCCAGGTCCGAGGCCGACGCGGGCCCGACCAACAACTGGGT
CGACCCGGCCGAGATGCGCGCCACCATGACCGAGCTCTACCGCGGCTGCATGAAGGGCCGCACCATGTACGTGGTGCCGT
TCTGCATGGGCCCGCTCGGCGCCGAGGATCCCAAGCTCGGCGTGGAGATCACCGACTCGGAGTACGTCGTGGTCTCGATG
CGCGTGATGACCCGCATGGGCAAGGCCGCGCTGGAGAAGATGGGCACCGATCGCCCGTTCGTCAAGGCCCTGCATTCGGT
GGGCGCTCCGCTGGCCGAGGGCCAGCAGGACGTGCCGTGGCCGTGCAACGACACCAAGTACATCACCCACTTCCCGGAGG
ATCGCGAGATCTGGAGCTACGGCTCCGGTTACGGCGGCAACGCGCTGCTCGGCAAGAAGTGCTACTCGCTGCGCATCGCC
TCGGCGATGGCGCACGACGAGGGCTGGCTGGCCGAGCACATGCTGATCCTCAAGCTGATCTCCCCGGAGAACAAGGCCTA
CTACATCGCCGCCGCCTTCCCCAGCGCCTGCGGTAAGACCAACCTCGCGATGATCCAGCCCACGGTTCCGGGCTGGCGCG
CCGAGACCCTCGGTGACGACATCGCCTGGATGCGCTTCGGCAAGGACGGCCGCCTCTACGCGGTGAACCCGGAGTTCGGT
TTCTTCGGCGTCGCGCCGGGCACCAACCACAGCTCCAACCCGAACGCGATGGCGACCATGGAGGCGGGCAACACCGTCTA
CACCAACGTCGCGCTCACCGACGACAACGATGTGTGGTGGGAGGGCCTGGAGGGCGAGCCCGACCACCTGATCGACTGGA
AGGGCAACGACTGGTACCTGCGCGAGACGGAGACGCTGGCCGCGCACCCGAATTCGCGCTACTGCACCCCGATGTCGCAG
TGCCCGATCCTGGCCCCGGAATGGGACGACCCGCAGGGCGTGCCGATCTCGGCGATCCTGTTCGGCGGCCGCCGCAAGAC
CACGGTCCCGCTGGTGACCGAGTCCTTCGACTGGCAGCACGGCGTGTTCATGGGCGCCACCCTGTCCTCGGAGCAGACCG
CCGCGGCCGAGGGCAAGGTCGGCACCGTGCGCCGCGATCCGATGGCGATGCTGCCGTTCCTGGGCTACCACGTGGGCGAC
TACCTCGACCACTGGATCCAGCTCGGCAAGAGCGCCGACGCGACCAAGCTGCCGAAGATCTTCTACGTCAACTGGTTCCG
TCGCGGCGAGGACGGCCGCTTCCTGTGGCCGGGCTTCGGTGAGAACTCGCGCGTGCTCGAGTGGATCGTCGGCCGGATCG
AGGGCAGCGCCGACGCCGAGGAGACCGCGATCGGCAACGTGCCAAGGGCCGCGCATCTGGATCTCGACGGCCTCGACGTC
GACCCCGCCGACGTCGACGAGGCGCTGGCCGTGAAGGCCGAGGAATGGCGCAAGGAGATCCCGCTGATCGAGGAGTGGTT
CGAGTTCGTCGGCGACAAGCTGCCCAGCGGCGTGCGTGACGAATTCGAGGCGCTGAAGCAGCGTCTGGGCTGA

Upstream 100 bases:

>100_bases
GTGCCTTCCCGCCAACGGTGGCGCTCGGTGTGTTCGCGCTGGTCATGGGCCTTGTGCCAGGGCGGTGCATCGCGGAGCCG
CCCATCTCAGGAGAGTTTTC

Downstream 100 bases:

>100_bases
CCCATCCGGACCAGCGGCGGAACCCCGGAGCATCGTGCTCCGGGGTTCCGCCGTTTCCGGCGGTTGTTTGATCACCGCGA
CCGGCGGTATTCCCCGCTCA

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 610; Mature: 609

Protein sequence:

>610_residues
MTSATIPGLRGSDGQAPTEHSELLAWVQEVAELTQPERVVWADGSDEEWERLTDQLVEAGTFKRLDPKKKPNSFLALSDP
SDVARVESRTFICSRSEADAGPTNNWVDPAEMRATMTELYRGCMKGRTMYVVPFCMGPLGAEDPKLGVEITDSEYVVVSM
RVMTRMGKAALEKMGTDRPFVKALHSVGAPLAEGQQDVPWPCNDTKYITHFPEDREIWSYGSGYGGNALLGKKCYSLRIA
SAMAHDEGWLAEHMLILKLISPENKAYYIAAAFPSACGKTNLAMIQPTVPGWRAETLGDDIAWMRFGKDGRLYAVNPEFG
FFGVAPGTNHSSNPNAMATMEAGNTVYTNVALTDDNDVWWEGLEGEPDHLIDWKGNDWYLRETETLAAHPNSRYCTPMSQ
CPILAPEWDDPQGVPISAILFGGRRKTTVPLVTESFDWQHGVFMGATLSSEQTAAAEGKVGTVRRDPMAMLPFLGYHVGD
YLDHWIQLGKSADATKLPKIFYVNWFRRGEDGRFLWPGFGENSRVLEWIVGRIEGSADAEETAIGNVPRAAHLDLDGLDV
DPADVDEALAVKAEEWRKEIPLIEEWFEFVGDKLPSGVRDEFEALKQRLG

Sequences:

>Translated_610_residues
MTSATIPGLRGSDGQAPTEHSELLAWVQEVAELTQPERVVWADGSDEEWERLTDQLVEAGTFKRLDPKKKPNSFLALSDP
SDVARVESRTFICSRSEADAGPTNNWVDPAEMRATMTELYRGCMKGRTMYVVPFCMGPLGAEDPKLGVEITDSEYVVVSM
RVMTRMGKAALEKMGTDRPFVKALHSVGAPLAEGQQDVPWPCNDTKYITHFPEDREIWSYGSGYGGNALLGKKCYSLRIA
SAMAHDEGWLAEHMLILKLISPENKAYYIAAAFPSACGKTNLAMIQPTVPGWRAETLGDDIAWMRFGKDGRLYAVNPEFG
FFGVAPGTNHSSNPNAMATMEAGNTVYTNVALTDDNDVWWEGLEGEPDHLIDWKGNDWYLRETETLAAHPNSRYCTPMSQ
CPILAPEWDDPQGVPISAILFGGRRKTTVPLVTESFDWQHGVFMGATLSSEQTAAAEGKVGTVRRDPMAMLPFLGYHVGD
YLDHWIQLGKSADATKLPKIFYVNWFRRGEDGRFLWPGFGENSRVLEWIVGRIEGSADAEETAIGNVPRAAHLDLDGLDV
DPADVDEALAVKAEEWRKEIPLIEEWFEFVGDKLPSGVRDEFEALKQRLG
>Mature_609_residues
TSATIPGLRGSDGQAPTEHSELLAWVQEVAELTQPERVVWADGSDEEWERLTDQLVEAGTFKRLDPKKKPNSFLALSDPS
DVARVESRTFICSRSEADAGPTNNWVDPAEMRATMTELYRGCMKGRTMYVVPFCMGPLGAEDPKLGVEITDSEYVVVSMR
VMTRMGKAALEKMGTDRPFVKALHSVGAPLAEGQQDVPWPCNDTKYITHFPEDREIWSYGSGYGGNALLGKKCYSLRIAS
AMAHDEGWLAEHMLILKLISPENKAYYIAAAFPSACGKTNLAMIQPTVPGWRAETLGDDIAWMRFGKDGRLYAVNPEFGF
FGVAPGTNHSSNPNAMATMEAGNTVYTNVALTDDNDVWWEGLEGEPDHLIDWKGNDWYLRETETLAAHPNSRYCTPMSQC
PILAPEWDDPQGVPISAILFGGRRKTTVPLVTESFDWQHGVFMGATLSSEQTAAAEGKVGTVRRDPMAMLPFLGYHVGDY
LDHWIQLGKSADATKLPKIFYVNWFRRGEDGRFLWPGFGENSRVLEWIVGRIEGSADAEETAIGNVPRAAHLDLDGLDVD
PADVDEALAVKAEEWRKEIPLIEEWFEFVGDKLPSGVRDEFEALKQRLG

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family

Homologues:

Organism=Homo sapiens, GI187281517, Length=616, Percent_Identity=51.2987012987013, Blast_Score=607, Evalue=1e-174,
Organism=Homo sapiens, GI66346721, Length=621, Percent_Identity=50.8856682769726, Blast_Score=603, Evalue=1e-172,
Organism=Homo sapiens, GI66346723, Length=385, Percent_Identity=51.9480519480519, Blast_Score=380, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI71989645, Length=621, Percent_Identity=47.5040257648953, Blast_Score=584, Evalue=1e-167,
Organism=Caenorhabditis elegans, GI71989658, Length=621, Percent_Identity=47.5040257648953, Blast_Score=583, Evalue=1e-167,
Organism=Caenorhabditis elegans, GI17555492, Length=611, Percent_Identity=49.0998363338789, Blast_Score=578, Evalue=1e-165,
Organism=Caenorhabditis elegans, GI71989653, Length=592, Percent_Identity=48.3108108108108, Blast_Score=574, Evalue=1e-164,
Organism=Caenorhabditis elegans, GI32564411, Length=543, Percent_Identity=51.5653775322284, Blast_Score=549, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI32564407, Length=536, Percent_Identity=50.5597014925373, Blast_Score=533, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI71989649, Length=523, Percent_Identity=47.8011472275335, Blast_Score=497, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI32564409, Length=369, Percent_Identity=50.6775067750677, Blast_Score=367, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17540956, Length=492, Percent_Identity=23.9837398373984, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24655085, Length=619, Percent_Identity=50.4038772213247, Blast_Score=608, Evalue=1e-174,
Organism=Drosophila melanogaster, GI24655082, Length=599, Percent_Identity=51.5859766277129, Blast_Score=596, Evalue=1e-170,
Organism=Drosophila melanogaster, GI161077226, Length=556, Percent_Identity=52.8776978417266, Blast_Score=573, Evalue=1e-164,
Organism=Drosophila melanogaster, GI24655088, Length=504, Percent_Identity=53.1746031746032, Blast_Score=543, Evalue=1e-154,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PCKG_NOCFA (Q5YNB0)

Other databases:

- EMBL:   AP006618
- RefSeq:   YP_121695.1
- ProteinModelPortal:   Q5YNB0
- SMR:   Q5YNB0
- GeneID:   3111363
- GenomeReviews:   AP006618_GR
- KEGG:   nfa:nfa54790
- NMPDR:   fig|247156.1.peg.5266
- HOGENOM:   HBG484437
- OMA:   CGYHMGD
- ProtClustDB:   PRK04210
- BioCyc:   NFAR247156:NFA54790-MONOMER
- BRENDA:   4.1.1.32
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00452
- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PANTHER:   PTHR11561
- PIRSF:   PIRSF001348

Pfam domain/function: PF00821 PEPCK; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.32

Molecular weight: Translated: 67711; Mature: 67580

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00505 PEPCK_GTP

Important sites: ACT_SITE 277-277 BINDING 85-85 BINDING 226-226 BINDING 233-233 BINDING 275-275 BINDING 393-393 BINDING 424-424

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSATIPGLRGSDGQAPTEHSELLAWVQEVAELTQPERVVWADGSDEEWERLTDQLVEAG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCC
TFKRLDPKKKPNSFLALSDPSDVARVESRTFICSRSEADAGPTNNWVDPAEMRATMTELY
CCCCCCCCCCCCCEEEECCCHHHHHHHCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHH
RGCMKGRTMYVVPFCMGPLGAEDPKLGVEITDSEYVVVSMRVMTRMGKAALEKMGTDRPF
HHHHCCCEEEEEEEECCCCCCCCCCCCEEECCCCEEEEEHHHHHHHHHHHHHHCCCCCHH
VKALHSVGAPLAEGQQDVPWPCNDTKYITHFPEDREIWSYGSGYGGNALLGKKCYSLRIA
HHHHHHHCCCHHCCCCCCCCCCCCCCEEEECCCCCCHHCCCCCCCCCCHHHHHHHHHHHH
SAMAHDEGWLAEHMLILKLISPENKAYYIAAAFPSACGKTNLAMIQPTVPGWRAETLGDD
HHHHCCCCHHHHHHHHHHEECCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCHHHCCCC
IAWMRFGKDGRLYAVNPEFGFFGVAPGTNHSSNPNAMATMEAGNTVYTNVALTDDNDVWW
EEEEEECCCCCEEEECCCCCEEECCCCCCCCCCCCEEEEEECCCEEEEEEEEECCCCCEE
EGLEGEPDHLIDWKGNDWYLRETETLAAHPNSRYCTPMSQCPILAPEWDDPQGVPISAIL
ECCCCCCCCEEEECCCCEEEEECCEEEECCCCCCCCCHHHCCEECCCCCCCCCCEEEEEE
FGGRRKTTVPLVTESFDWQHGVFMGATLSSEQTAAAEGKVGTVRRDPMAMLPFLGYHVGD
ECCCCCCEEEEEECCCCCCCCEEEEEECCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHH
YLDHWIQLGKSADATKLPKIFYVNWFRRGEDGRFLWPGFGENSRVLEWIVGRIEGSADAE
HHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCEECCCCCCCCHHHHHHHHHHCCCCCCH
ETAIGNVPRAAHLDLDGLDVDPADVDEALAVKAEEWRKEIPLIEEWFEFVGDKLPSGVRD
HHHCCCCCCEEECCCCCCCCCCCCCHHHHHHCHHHHHHCCCHHHHHHHHHHHHCCCCHHH
EFEALKQRLG
HHHHHHHHCC
>Mature Secondary Structure 
TSATIPGLRGSDGQAPTEHSELLAWVQEVAELTQPERVVWADGSDEEWERLTDQLVEAG
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCC
TFKRLDPKKKPNSFLALSDPSDVARVESRTFICSRSEADAGPTNNWVDPAEMRATMTELY
CCCCCCCCCCCCCEEEECCCHHHHHHHCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHH
RGCMKGRTMYVVPFCMGPLGAEDPKLGVEITDSEYVVVSMRVMTRMGKAALEKMGTDRPF
HHHHCCCEEEEEEEECCCCCCCCCCCCEEECCCCEEEEEHHHHHHHHHHHHHHCCCCCHH
VKALHSVGAPLAEGQQDVPWPCNDTKYITHFPEDREIWSYGSGYGGNALLGKKCYSLRIA
HHHHHHHCCCHHCCCCCCCCCCCCCCEEEECCCCCCHHCCCCCCCCCCHHHHHHHHHHHH
SAMAHDEGWLAEHMLILKLISPENKAYYIAAAFPSACGKTNLAMIQPTVPGWRAETLGDD
HHHHCCCCHHHHHHHHHHEECCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCHHHCCCC
IAWMRFGKDGRLYAVNPEFGFFGVAPGTNHSSNPNAMATMEAGNTVYTNVALTDDNDVWW
EEEEEECCCCCEEEECCCCCEEECCCCCCCCCCCCEEEEEECCCEEEEEEEEECCCCCEE
EGLEGEPDHLIDWKGNDWYLRETETLAAHPNSRYCTPMSQCPILAPEWDDPQGVPISAIL
ECCCCCCCCEEEECCCCEEEEECCEEEECCCCCCCCCHHHCCEECCCCCCCCCCEEEEEE
FGGRRKTTVPLVTESFDWQHGVFMGATLSSEQTAAAEGKVGTVRRDPMAMLPFLGYHVGD
ECCCCCCEEEEEECCCCCCCCEEEEEECCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHH
YLDHWIQLGKSADATKLPKIFYVNWFRRGEDGRFLWPGFGENSRVLEWIVGRIEGSADAE
HHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCEECCCCCCCCHHHHHHHHHHCCCCCCH
ETAIGNVPRAAHLDLDGLDVDPADVDEALAVKAEEWRKEIPLIEEWFEFVGDKLPSGVRD
HHHCCCCCCEEECCCCCCCCCCCCCHHHHHHCHHHHHHCCCHHHHHHHHHHHHCCCCHHH
EFEALKQRLG
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA