Click here to switch to the map view.

The map label for this gene is purA

Identifier: 54027347

GI number: 54027347

Start: 5677992

End: 5679281

Strand: Reverse

Name: purA

Synonym: nfa53730

Alternate gene names: 54027347

Gene position: 5679281-5677992 (Counterclockwise)

Preceding gene: 54027350

Following gene: 54027346

Centisome position: 94.32

GC content: 68.14

Gene sequence:

>1290_bases
ATGCCGGCAATCGTCCTCATCGGCGCCCAATGGGGCGACGAGGGTAAGGGCAAAGCGACCGATCTGCTCGGTGGCCGGTT
GCAATGGGTGGTGCGGTACCAGGGCGGCAACAACGCCGGACACACCGTCGTGCTGCCCAACGGCGACAAGTTCGCCCTGC
ACCTCATCCCGTCCGGCATCCTGACCCCCGGCGTGCGCAACGTCATCGGCAACGGTGTCGTGGTCGACCCGGGCGTGCTG
CTCGACGAACTCGCCGGCCTCGAGGCCCGCGACGTCGACACCTCGGGCCTGCTGCTCTCCGCCGACGCGCACCTGATCAT
GCCGTACCACGTGGCCATCGATAAGGTCACCGAACGCTTCCTCGGCAACAAGAAGATCGGCACCACCGGCCGCGGCATCG
GCCCCTGCTACCAGGACAAGGTGGCGCGGGTCGGGGTGCGGGTGGCCGACGTGCTGGACGAGAAGATCCTCACCCAGAAG
GTCGAGGCCGCCCTGGAGTTCAAGAACCAGGTGCTGGTGAAGATCTACAACCGTCGCGCGCTGGACCCGCAGCAGGTGGT
CGACGAAGTGCTCGGTCAGGCCGAGAGCTTCGCCCACCGCATCGCCGACACCCGGTTGCAGCTCAACGAGGCGCTCGAGC
GCGGCGAGACCGTGCTGCTGGAAGGCTCGCAGGGCACCCTGCTCGACGTCGACCACGGCACCTATCCCTATGTGACGTCG
TCGAACCCGACCTCGGGCGGCGCGGCGGTCGGTTCCGGCATCGGGCCGAACAAGATCAGCACGGTGCTGGGCATCCTCAA
GTGCTACACCACCCGGGTCGGCTCCGGCCCGTTCCCGACCGAGTTGTTCGACCAGTACGGCGAGTTCCTCGCCAAGCAGG
GCGGCGAGGTCGGCGTCACCACCGGGCGCGCTCGCCGCTGCGGCTGGTTCGACGCGGTGATCGCGCGCTACGCCACCCGC
GTCAACGGCATCACCGACTACTTCCTCACCAAGCTCGACGTGCTCTCCAGCCTGGAGCGGGTGCCGATCTGCGTGGCCTA
CGAGATCGACGGGCAGCGGGTGGAGCAGATGCCGACCACGCAGACCGAATTCCACCACGCCAAGCCGATCTACGAGGAGA
TGCCGGGCTGGTGGGAGGACATCTCCGCGGCCCGCACCTTCGAGGAACTGCCCGCCAACGCGCAGGCCTACGTGCGCAGG
CTCGAGGAGCTGTCCGGTGCCCGGATCTCCTGCATCGGGGTGGGTCCGGGCCGCGATCAGACGATCGTGCGCCACGACGT
GCTCGGCTGA

Upstream 100 bases:

>100_bases
CGCGGGGCCATTGGGTAAGCTGTGCTTCCGGCCACCGCCTCAGTACGGCAGGTAGCCGCAGCACACGAGTGCTGCGTGTC
GATCCGTAGGAGACTCCACA

Downstream 100 bases:

>100_bases
GCCACGCCGGGCTCGGCCCGGCCTGCGCCGAGTTCCGCGCGCGCGGGGCGGTGCCGAACTACAGTTGGTACATGGCTCGG
AATTGGCCGATGATCGAGCG

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MPAIVLIGAQWGDEGKGKATDLLGGRLQWVVRYQGGNNAGHTVVLPNGDKFALHLIPSGILTPGVRNVIGNGVVVDPGVL
LDELAGLEARDVDTSGLLLSADAHLIMPYHVAIDKVTERFLGNKKIGTTGRGIGPCYQDKVARVGVRVADVLDEKILTQK
VEAALEFKNQVLVKIYNRRALDPQQVVDEVLGQAESFAHRIADTRLQLNEALERGETVLLEGSQGTLLDVDHGTYPYVTS
SNPTSGGAAVGSGIGPNKISTVLGILKCYTTRVGSGPFPTELFDQYGEFLAKQGGEVGVTTGRARRCGWFDAVIARYATR
VNGITDYFLTKLDVLSSLERVPICVAYEIDGQRVEQMPTTQTEFHHAKPIYEEMPGWWEDISAARTFEELPANAQAYVRR
LEELSGARISCIGVGPGRDQTIVRHDVLG

Sequences:

>Translated_429_residues
MPAIVLIGAQWGDEGKGKATDLLGGRLQWVVRYQGGNNAGHTVVLPNGDKFALHLIPSGILTPGVRNVIGNGVVVDPGVL
LDELAGLEARDVDTSGLLLSADAHLIMPYHVAIDKVTERFLGNKKIGTTGRGIGPCYQDKVARVGVRVADVLDEKILTQK
VEAALEFKNQVLVKIYNRRALDPQQVVDEVLGQAESFAHRIADTRLQLNEALERGETVLLEGSQGTLLDVDHGTYPYVTS
SNPTSGGAAVGSGIGPNKISTVLGILKCYTTRVGSGPFPTELFDQYGEFLAKQGGEVGVTTGRARRCGWFDAVIARYATR
VNGITDYFLTKLDVLSSLERVPICVAYEIDGQRVEQMPTTQTEFHHAKPIYEEMPGWWEDISAARTFEELPANAQAYVRR
LEELSGARISCIGVGPGRDQTIVRHDVLG
>Mature_428_residues
PAIVLIGAQWGDEGKGKATDLLGGRLQWVVRYQGGNNAGHTVVLPNGDKFALHLIPSGILTPGVRNVIGNGVVVDPGVLL
DELAGLEARDVDTSGLLLSADAHLIMPYHVAIDKVTERFLGNKKIGTTGRGIGPCYQDKVARVGVRVADVLDEKILTQKV
EAALEFKNQVLVKIYNRRALDPQQVVDEVLGQAESFAHRIADTRLQLNEALERGETVLLEGSQGTLLDVDHGTYPYVTSS
NPTSGGAAVGSGIGPNKISTVLGILKCYTTRVGSGPFPTELFDQYGEFLAKQGGEVGVTTGRARRCGWFDAVIARYATRV
NGITDYFLTKLDVLSSLERVPICVAYEIDGQRVEQMPTTQTEFHHAKPIYEEMPGWWEDISAARTFEELPANAQAYVRRL
EELSGARISCIGVGPGRDQTIVRHDVLG

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI34577063, Length=434, Percent_Identity=41.9354838709677, Blast_Score=314, Evalue=1e-85,
Organism=Homo sapiens, GI22748717, Length=426, Percent_Identity=42.018779342723, Blast_Score=311, Evalue=6e-85,
Organism=Homo sapiens, GI40316944, Length=397, Percent_Identity=40.8060453400504, Blast_Score=279, Evalue=4e-75,
Organism=Escherichia coli, GI1790620, Length=425, Percent_Identity=51.5294117647059, Blast_Score=437, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI25146553, Length=438, Percent_Identity=43.37899543379, Blast_Score=328, Evalue=3e-90,
Organism=Caenorhabditis elegans, GI25146555, Length=432, Percent_Identity=43.5185185185185, Blast_Score=328, Evalue=4e-90,
Organism=Saccharomyces cerevisiae, GI6324109, Length=430, Percent_Identity=39.5348837209302, Blast_Score=327, Evalue=2e-90,
Organism=Drosophila melanogaster, GI21356033, Length=425, Percent_Identity=42.1176470588235, Blast_Score=330, Evalue=8e-91,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_NOCFA (Q5YNL6)

Other databases:

- EMBL:   AP006618
- RefSeq:   YP_121589.1
- ProteinModelPortal:   Q5YNL6
- SMR:   Q5YNL6
- GeneID:   3111412
- GenomeReviews:   AP006618_GR
- KEGG:   nfa:nfa53730
- NMPDR:   fig|247156.1.peg.5166
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- ProtClustDB:   PRK01117
- BioCyc:   NFAR247156:NFA53730-MONOMER
- BRENDA:   6.3.4.4
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 46455; Mature: 46324

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 129-129 BINDING 143-143 BINDING 224-224 BINDING 239-239 BINDING 303-303 BINDING 305-305

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAIVLIGAQWGDEGKGKATDLLGGRLQWVVRYQGGNNAGHTVVLPNGDKFALHLIPSGI
CCEEEEEECCCCCCCCCCHHHHCCCEEEEEEEECCCCCCCCEEEEECCCEEEEEECCCCC
LTPGVRNVIGNGVVVDPGVLLDELAGLEARDVDTSGLLLSADAHLIMPYHVAIDKVTERF
CCCCHHHHHCCCEEECCCHHHHHHCCCCCCCCCCCCEEEECCCEEEEHHHHHHHHHHHHH
LGNKKIGTTGRGIGPCYQDKVARVGVRVADVLDEKILTQKVEAALEFKNQVLVKIYNRRA
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LDPQQVVDEVLGQAESFAHRIADTRLQLNEALERGETVLLEGSQGTLLDVDHGTYPYVTS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEEEC
SNPTSGGAAVGSGIGPNKISTVLGILKCYTTRVGSGPFPTELFDQYGEFLAKQGGEVGVT
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEC
TGRARRCGWFDAVIARYATRVNGITDYFLTKLDVLSSLERVPICVAYEIDGQRVEQMPTT
CCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHCCCC
QTEFHHAKPIYEEMPGWWEDISAARTFEELPANAQAYVRRLEELSGARISCIGVGPGRDQ
HHHHHCCCCHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCCCCCC
TIVRHDVLG
EEHHHCCCC
>Mature Secondary Structure 
PAIVLIGAQWGDEGKGKATDLLGGRLQWVVRYQGGNNAGHTVVLPNGDKFALHLIPSGI
CEEEEEECCCCCCCCCCHHHHCCCEEEEEEEECCCCCCCCEEEEECCCEEEEEECCCCC
LTPGVRNVIGNGVVVDPGVLLDELAGLEARDVDTSGLLLSADAHLIMPYHVAIDKVTERF
CCCCHHHHHCCCEEECCCHHHHHHCCCCCCCCCCCCEEEECCCEEEEHHHHHHHHHHHHH
LGNKKIGTTGRGIGPCYQDKVARVGVRVADVLDEKILTQKVEAALEFKNQVLVKIYNRRA
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LDPQQVVDEVLGQAESFAHRIADTRLQLNEALERGETVLLEGSQGTLLDVDHGTYPYVTS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEEEC
SNPTSGGAAVGSGIGPNKISTVLGILKCYTTRVGSGPFPTELFDQYGEFLAKQGGEVGVT
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEC
TGRARRCGWFDAVIARYATRVNGITDYFLTKLDVLSSLERVPICVAYEIDGQRVEQMPTT
CCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHCCCC
QTEFHHAKPIYEEMPGWWEDISAARTFEELPANAQAYVRRLEELSGARISCIGVGPGRDQ
HHHHHCCCCHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCCCCCC
TIVRHDVLG
EEHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA