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The map label for this gene is hemL

Identifier: 54027134

GI number: 54027134

Start: 5460382

End: 5461719

Strand: Reverse

Name: hemL

Synonym: nfa51600

Alternate gene names: 54027134

Gene position: 5461719-5460382 (Counterclockwise)

Preceding gene: 54027136

Following gene: 54027133

Centisome position: 90.71

GC content: 73.17

Gene sequence:

>1338_bases
GTGAGTGCTTCTCCGCGCGCGACCCATGCCTCGGCGGCGGCTTCGGCCCGGCTCTTCGACCGGGCCGCCCAGGTGATCCC
CGGCGGCGTCAACTCGCCGGTGCGGGCCTTCCGATCGGTCGGCGGCACCCCCCGCTTCATCGCTTCGGCCGACGGCTACA
CCCTCACCGACGCCGACGGCAACGAGTATGTCGACCTGGTGTGTTCGTGGGGCCCGATGATTCTCGGGCACGCGCACCCC
GCGGTGGTCGACGCGGTGCGGCGGGCGGCCACCGGCGGCCTGTCCTTCGGTGCGCCGACCGAGGCCGAGGTGGAGCTGGC
CGAGCACATCGTGGCGCGGGTCGCCCCGGTGGACCGCGTCCGGCTGGTCAACTCCGGCACCGAAGCCACCATGAGCGCGG
TCCGGCTGGCCCGCGGTTTCACCGGCCGCACCAAGATCATCAAGTTCGCCGGCTGCTACCACGGGCACGTGGACGCGCTG
CTCGCCGACGCGGGTTCGGGTGTGGCGACCCTCGGGCTACCGACCTCGCCCGGTGTCACCGGCGCCCAGGCGGCCGACAC
GATCGTGCTGCCCTACAACGACCTGGACGCCGTCGCCGCGGCCTTCGAGGCCAACCCCGGCGAGATCGCCTGCGTCATCA
CCGAGGCCGCCGCGGGCAACATGGGCGCGGTCGCACCACTGCCCGGTTTCAACGCGGGCCTGCGCGAACTCACCGAGAAC
CACGGCGCCCTGTTGATCATGGACGAGGTGATGACGGGCTTCCGGGTCAGCCGCTCGGGCTGGTACGGCCGCGAGGGCGT
GGCGGGCGACCTGTACACCTTCGGCAAGGTGATGAGCGGTGGACTGCCCGCCGCCGCGTTCGGCGGACGCGCCGAGGTGA
TGAACCAGCTGGCTCCGCTCGGGCCGGTGTACCAGGCGGGCACGCTGTCGGGTAACCCGGTGGCCGTCGCCGCGGGCCTG
GCCACGCTGCGCGCCGCCGACGACGCGGTCTACGCCGCGCTGGATCGCAACGCCGAGCGGCTCGGCGGACTGCTCACCGA
GGCGCTCACCGCGGCCGGGGTCGAGCACCAGGTCCAGTTCGCGGGCAATATGGTGAGCGTGTTCTTCAGCGACCGCCCGG
TCACCGACTACGCCACCGCCAAGGCCAGCCAGACCTGGCGTTTCCCCGCCTTCTTCCACGCGCTGCTCGACGGCGGTGTG
TACGCGCCGCCGAGCGCGTTCGAGGCGTGGTTCGTCTCCGCGGCGCTCGACGAGGACGCATTCGCCCGTATCGCCGCAGC
CCTGCCCGCCGCCGCTCGCGCGGCCGCGGCCGCCACCCCCGAAGGATCCCGCGCGTGA

Upstream 100 bases:

>100_bases
AATCGGCGGAAACGCCCACCCGGCGCGTCGCTGCGGTGCGGGTCACCTCTCCTACACCCTGTCGTCGACGGCTTTCCGGC
GGGCTGCGACACTGGAGGCC

Downstream 100 bases:

>100_bases
GTCCCGACCACGACCAGCCCGCCGAGCGCCGCGAACCACTCGAGGCCGACAACCCCGCCGCCGACACCACCGCCGACCTG
ACCGTCGCCCCGCCCGCCGC

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MSASPRATHASAAASARLFDRAAQVIPGGVNSPVRAFRSVGGTPRFIASADGYTLTDADGNEYVDLVCSWGPMILGHAHP
AVVDAVRRAATGGLSFGAPTEAEVELAEHIVARVAPVDRVRLVNSGTEATMSAVRLARGFTGRTKIIKFAGCYHGHVDAL
LADAGSGVATLGLPTSPGVTGAQAADTIVLPYNDLDAVAAAFEANPGEIACVITEAAAGNMGAVAPLPGFNAGLRELTEN
HGALLIMDEVMTGFRVSRSGWYGREGVAGDLYTFGKVMSGGLPAAAFGGRAEVMNQLAPLGPVYQAGTLSGNPVAVAAGL
ATLRAADDAVYAALDRNAERLGGLLTEALTAAGVEHQVQFAGNMVSVFFSDRPVTDYATAKASQTWRFPAFFHALLDGGV
YAPPSAFEAWFVSAALDEDAFARIAAALPAAARAAAAATPEGSRA

Sequences:

>Translated_445_residues
MSASPRATHASAAASARLFDRAAQVIPGGVNSPVRAFRSVGGTPRFIASADGYTLTDADGNEYVDLVCSWGPMILGHAHP
AVVDAVRRAATGGLSFGAPTEAEVELAEHIVARVAPVDRVRLVNSGTEATMSAVRLARGFTGRTKIIKFAGCYHGHVDAL
LADAGSGVATLGLPTSPGVTGAQAADTIVLPYNDLDAVAAAFEANPGEIACVITEAAAGNMGAVAPLPGFNAGLRELTEN
HGALLIMDEVMTGFRVSRSGWYGREGVAGDLYTFGKVMSGGLPAAAFGGRAEVMNQLAPLGPVYQAGTLSGNPVAVAAGL
ATLRAADDAVYAALDRNAERLGGLLTEALTAAGVEHQVQFAGNMVSVFFSDRPVTDYATAKASQTWRFPAFFHALLDGGV
YAPPSAFEAWFVSAALDEDAFARIAAALPAAARAAAAATPEGSRA
>Mature_444_residues
SASPRATHASAAASARLFDRAAQVIPGGVNSPVRAFRSVGGTPRFIASADGYTLTDADGNEYVDLVCSWGPMILGHAHPA
VVDAVRRAATGGLSFGAPTEAEVELAEHIVARVAPVDRVRLVNSGTEATMSAVRLARGFTGRTKIIKFAGCYHGHVDALL
ADAGSGVATLGLPTSPGVTGAQAADTIVLPYNDLDAVAAAFEANPGEIACVITEAAAGNMGAVAPLPGFNAGLRELTENH
GALLIMDEVMTGFRVSRSGWYGREGVAGDLYTFGKVMSGGLPAAAFGGRAEVMNQLAPLGPVYQAGTLSGNPVAVAAGLA
TLRAADDAVYAALDRNAERLGGLLTEALTAAGVEHQVQFAGNMVSVFFSDRPVTDYATAKASQTWRFPAFFHALLDGGVY
APPSAFEAWFVSAALDEDAFARIAAALPAAARAAAAATPEGSRA

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=319, Percent_Identity=28.8401253918495, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI37574042, Length=332, Percent_Identity=28.6144578313253, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI226442709, Length=301, Percent_Identity=28.9036544850498, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI226442705, Length=331, Percent_Identity=27.4924471299094, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI24119277, Length=359, Percent_Identity=26.4623955431755, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI284507298, Length=294, Percent_Identity=27.891156462585, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1786349, Length=405, Percent_Identity=57.5308641975309, Blast_Score=439, Evalue=1e-124,
Organism=Escherichia coli, GI1789016, Length=379, Percent_Identity=33.245382585752, Blast_Score=135, Evalue=7e-33,
Organism=Escherichia coli, GI1787560, Length=391, Percent_Identity=31.7135549872123, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1789759, Length=276, Percent_Identity=33.3333333333333, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI145693181, Length=344, Percent_Identity=27.6162790697674, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1788044, Length=278, Percent_Identity=31.6546762589928, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1786991, Length=251, Percent_Identity=27.8884462151394, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71992977, Length=409, Percent_Identity=27.1393643031785, Blast_Score=118, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI25144271, Length=357, Percent_Identity=26.6106442577031, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323470, Length=387, Percent_Identity=24.8062015503876, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324432, Length=313, Percent_Identity=26.517571884984, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21357415, Length=319, Percent_Identity=25.0783699059561, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_NOCFA (Q5YP79)

Other databases:

- EMBL:   AP006618
- RefSeq:   YP_121376.1
- ProteinModelPortal:   Q5YP79
- SMR:   Q5YP79
- GeneID:   3109563
- GenomeReviews:   AP006618_GR
- KEGG:   nfa:nfa51600
- NMPDR:   fig|247156.1.peg.4956
- HOGENOM:   HBG725944
- OMA:   VGCFGGK
- ProtClustDB:   PRK00062
- BioCyc:   NFAR247156:NFA51600-MONOMER
- BRENDA:   5.4.3.8
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 45485; Mature: 45353

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASPRATHASAAASARLFDRAAQVIPGGVNSPVRAFRSVGGTPRFIASADGYTLTDADG
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCEEECCCCCEEECCCC
NEYVDLVCSWGPMILGHAHPAVVDAVRRAATGGLSFGAPTEAEVELAEHIVARVAPVDRV
CCEEEEEECCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCHHHE
RLVNSGTEATMSAVRLARGFTGRTKIIKFAGCYHGHVDALLADAGSGVATLGLPTSPGVT
EEECCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHCCCCCEEEECCCCCCCCC
GAQAADTIVLPYNDLDAVAAAFEANPGEIACVITEAAAGNMGAVAPLPGFNAGLRELTEN
CCCCCCEEEECCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHC
HGALLIMDEVMTGFRVSRSGWYGREGVAGDLYTFGKVMSGGLPAAAFGGRAEVMNQLAPL
CCEEEEEHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCCHHHCCCHHHHHHHHCCC
GPVYQAGTLSGNPVAVAAGLATLRAADDAVYAALDRNAERLGGLLTEALTAAGVEHQVQF
CCCCCCCCCCCCCEEEHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHH
AGNMVSVFFSDRPVTDYATAKASQTWRFPAFFHALLDGGVYAPPSAFEAWFVSAALDEDA
CCCEEEEEECCCCCCHHHHHCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHH
FARIAAALPAAARAAAAATPEGSRA
HHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SASPRATHASAAASARLFDRAAQVIPGGVNSPVRAFRSVGGTPRFIASADGYTLTDADG
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCEEECCCCCEEECCCC
NEYVDLVCSWGPMILGHAHPAVVDAVRRAATGGLSFGAPTEAEVELAEHIVARVAPVDRV
CCEEEEEECCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCHHHE
RLVNSGTEATMSAVRLARGFTGRTKIIKFAGCYHGHVDALLADAGSGVATLGLPTSPGVT
EEECCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHCCCCCEEEECCCCCCCCC
GAQAADTIVLPYNDLDAVAAAFEANPGEIACVITEAAAGNMGAVAPLPGFNAGLRELTEN
CCCCCCEEEECCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHC
HGALLIMDEVMTGFRVSRSGWYGREGVAGDLYTFGKVMSGGLPAAAFGGRAEVMNQLAPL
CCEEEEEHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCCHHHCCCHHHHHHHHCCC
GPVYQAGTLSGNPVAVAAGLATLRAADDAVYAALDRNAERLGGLLTEALTAAGVEHQVQF
CCCCCCCCCCCCCEEEHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHH
AGNMVSVFFSDRPVTDYATAKASQTWRFPAFFHALLDGGVYAPPSAFEAWFVSAALDEDA
CCCEEEEEECCCCCCHHHHHCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHH
FARIAAALPAAARAAAAATPEGSRA
HHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA