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The map label for this gene is metB [H]

Identifier: 54026801

GI number: 54026801

Start: 5041407

End: 5042552

Strand: Reverse

Name: metB [H]

Synonym: nfa48270

Alternate gene names: 54026801

Gene position: 5042552-5041407 (Counterclockwise)

Preceding gene: 54026804

Following gene: 54026798

Centisome position: 83.75

GC content: 70.77

Gene sequence:

>1146_bases
ATGAGCGAGCTGGGATTCTCCACCAGGGCCGTGCACGCCGGATTCGATCCCGATCCCCAGACCGGCGCGGTGAACGTGCC
GATCTACGCGAGCTCGACGTTCGCCCAGGACGGTGTCGGCGGCCTGCGCGGCGGATTCGAGTACGCCCGGACCGGCAACC
CGACCCGCAGCGCCCTGGAGGCCAACCTGGCCGCCCTCGAATCGGGCGCGCACGGCCGGGCCTTCGCCTCCGGCATGGCC
GCCACCGACTGCGTGCTGCGCGCCACCCTGCGCCCCGGCGACCACATCGTCATCCCCGACGACGCCTACGGCGGCACCTT
CCGGCTGATCGACAAGGTCTTCAGCCAGTGGGGCATCGAGCACACCCCGGCCCACGTCTTCGACACCGACGCAATGCGCG
CGGCCATCCGCCCCAACACCAAGCTGGTGTGGGTGGAGACGCCGACCAATCCGCTGCTGAGCATCGGCGACATCCCGGCC
CTCGCCGAGATCGCGCACGCCGCGGGCGCGAAGCTGGTGGTGGACAACACCTTCGCGACCCCGTATCTGCAGCAGCCGCT
GGTGCTCGGCGCCGACGTGGTCGTGCACTCGACCACCAAGTACCTGGGCGGGCACTCCGACGTGGTGGGCGGCGCGCTGA
TCACCAACGATCCGGAGCTGGACGCGGCCTTCGGCTTCCTGCAGAACGGCGCGGGCGCGGTGCCCGGCCCGTTCGACGCC
TACCTCACCATGCGCGGCACCAAGACACTGGCCGTGCGGATGGAGCGGCACTGCGACAACGCCGAGGCCATCGCCGGTTT
CCTGGCGAAGCACCCGGCGGTATCCACGGTGATCTACCCGGGCCTGCCGGAGCATCCCGGCCACGCCGTCGCCGACAAGC
AGATGCGTCGTTTCGGCGGCATGATCTCGGTCCGGTTGCACGGCGGCCGGGACGCGGCACTGGACTTCTGCTCGCGGACG
AAGATCTTCACCCTCGCCGAGTCGCTGGGCGGGGTCGAGTCGCTGATCGAACACCCCGGCGCGATGACGCACGCCTCCAC
CGCGGGCTCCCAGCTCGAAGTGCCCGCGGACCTGGTGCGGCTGTCGGTCGGCATCGAGGACGCCGCGGATCTGCTCGCCG
ACGTCGAGCAGGCGCTGGCGAAGTAG

Upstream 100 bases:

>100_bases
GGCTGTCGATCGCGAAGAGTTCCTCCACCGTCACCATGCCCCGAGCCTAGAGTGGCCCCTCCGCCGTCGGGCGCCGATGG
CCGCCCTCTAGGCTGGTCGC

Downstream 100 bases:

>100_bases
GGGACCACGGAGATGACGAAGCGGCCGCACTCGAGACCGAGTGCGGCCGCTTGCGTACGGGCCGGGTTCGGGGGCTGTGA
CGGATCAGCCGTGGTAGGGC

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MSELGFSTRAVHAGFDPDPQTGAVNVPIYASSTFAQDGVGGLRGGFEYARTGNPTRSALEANLAALESGAHGRAFASGMA
ATDCVLRATLRPGDHIVIPDDAYGGTFRLIDKVFSQWGIEHTPAHVFDTDAMRAAIRPNTKLVWVETPTNPLLSIGDIPA
LAEIAHAAGAKLVVDNTFATPYLQQPLVLGADVVVHSTTKYLGGHSDVVGGALITNDPELDAAFGFLQNGAGAVPGPFDA
YLTMRGTKTLAVRMERHCDNAEAIAGFLAKHPAVSTVIYPGLPEHPGHAVADKQMRRFGGMISVRLHGGRDAALDFCSRT
KIFTLAESLGGVESLIEHPGAMTHASTAGSQLEVPADLVRLSVGIEDAADLLADVEQALAK

Sequences:

>Translated_381_residues
MSELGFSTRAVHAGFDPDPQTGAVNVPIYASSTFAQDGVGGLRGGFEYARTGNPTRSALEANLAALESGAHGRAFASGMA
ATDCVLRATLRPGDHIVIPDDAYGGTFRLIDKVFSQWGIEHTPAHVFDTDAMRAAIRPNTKLVWVETPTNPLLSIGDIPA
LAEIAHAAGAKLVVDNTFATPYLQQPLVLGADVVVHSTTKYLGGHSDVVGGALITNDPELDAAFGFLQNGAGAVPGPFDA
YLTMRGTKTLAVRMERHCDNAEAIAGFLAKHPAVSTVIYPGLPEHPGHAVADKQMRRFGGMISVRLHGGRDAALDFCSRT
KIFTLAESLGGVESLIEHPGAMTHASTAGSQLEVPADLVRLSVGIEDAADLLADVEQALAK
>Mature_380_residues
SELGFSTRAVHAGFDPDPQTGAVNVPIYASSTFAQDGVGGLRGGFEYARTGNPTRSALEANLAALESGAHGRAFASGMAA
TDCVLRATLRPGDHIVIPDDAYGGTFRLIDKVFSQWGIEHTPAHVFDTDAMRAAIRPNTKLVWVETPTNPLLSIGDIPAL
AEIAHAAGAKLVVDNTFATPYLQQPLVLGADVVVHSTTKYLGGHSDVVGGALITNDPELDAAFGFLQNGAGAVPGPFDAY
LTMRGTKTLAVRMERHCDNAEAIAGFLAKHPAVSTVIYPGLPEHPGHAVADKQMRRFGGMISVRLHGGRDAALDFCSRTK
IFTLAESLGGVESLIEHPGAMTHASTAGSQLEVPADLVRLSVGIEDAADLLADVEQALAK

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=379, Percent_Identity=49.0765171503958, Blast_Score=322, Evalue=4e-88,
Organism=Homo sapiens, GI299473759, Length=379, Percent_Identity=45.1187335092348, Blast_Score=278, Evalue=8e-75,
Organism=Homo sapiens, GI25453487, Length=378, Percent_Identity=42.5925925925926, Blast_Score=253, Evalue=3e-67,
Organism=Escherichia coli, GI1790375, Length=374, Percent_Identity=44.1176470588235, Blast_Score=296, Evalue=1e-81,
Organism=Escherichia coli, GI1789383, Length=401, Percent_Identity=29.1770573566085, Blast_Score=163, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17560056, Length=381, Percent_Identity=49.0813648293963, Blast_Score=338, Evalue=3e-93,
Organism=Caenorhabditis elegans, GI17538055, Length=381, Percent_Identity=48.5564304461942, Blast_Score=333, Evalue=9e-92,
Organism=Caenorhabditis elegans, GI71980708, Length=370, Percent_Identity=37.8378378378378, Blast_Score=249, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6319307, Length=386, Percent_Identity=49.2227979274611, Blast_Score=323, Evalue=2e-89,
Organism=Saccharomyces cerevisiae, GI6321254, Length=402, Percent_Identity=31.8407960199005, Blast_Score=168, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6321170, Length=341, Percent_Identity=31.3782991202346, Blast_Score=160, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6323333, Length=431, Percent_Identity=31.7865429234339, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6321904, Length=394, Percent_Identity=27.9187817258883, Blast_Score=136, Evalue=6e-33,
Organism=Drosophila melanogaster, GI19922554, Length=381, Percent_Identity=47.7690288713911, Blast_Score=348, Evalue=3e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 39935; Mature: 39803

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELGFSTRAVHAGFDPDPQTGAVNVPIYASSTFAQDGVGGLRGGFEYARTGNPTRSALE
CCCCCCCCEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCHHCCCCHHHCCCCCHHHHHH
ANLAALESGAHGRAFASGMAATDCVLRATLRPGDHIVIPDDAYGGTFRLIDKVFSQWGIE
HHHHHHHCCCCCHHHHCCCHHHHHHHEEECCCCCEEEECCCCCCCHHHHHHHHHHHCCCC
HTPAHVFDTDAMRAAIRPNTKLVWVETPTNPLLSIGDIPALAEIAHAAGAKLVVDNTFAT
CCCCCEECCHHHHHHCCCCCEEEEEECCCCCCEECCCCHHHHHHHHHCCCEEEEECCCCC
PYLQQPLVLGADVVVHSTTKYLGGHSDVVGGALITNDPELDAAFGFLQNGAGAVPGPFDA
HHHCCCHHCCCCEEEECHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHCCCCCCCCCCCE
YLTMRGTKTLAVRMERHCDNAEAIAGFLAKHPAVSTVIYPGLPEHPGHAVADKQMRRFGG
EEEECCCCEEEEEEHHCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHCC
MISVRLHGGRDAALDFCSRTKIFTLAESLGGVESLIEHPGAMTHASTAGSQLEVPADLVR
EEEEEEECCCHHHHHHHHCCHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCEECCHHHHH
LSVGIEDAADLLADVEQALAK
HHCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SELGFSTRAVHAGFDPDPQTGAVNVPIYASSTFAQDGVGGLRGGFEYARTGNPTRSALE
CCCCCCCEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCHHCCCCHHHCCCCCHHHHHH
ANLAALESGAHGRAFASGMAATDCVLRATLRPGDHIVIPDDAYGGTFRLIDKVFSQWGIE
HHHHHHHCCCCCHHHHCCCHHHHHHHEEECCCCCEEEECCCCCCCHHHHHHHHHHHCCCC
HTPAHVFDTDAMRAAIRPNTKLVWVETPTNPLLSIGDIPALAEIAHAAGAKLVVDNTFAT
CCCCCEECCHHHHHHCCCCCEEEEEECCCCCCEECCCCHHHHHHHHHCCCEEEEECCCCC
PYLQQPLVLGADVVVHSTTKYLGGHSDVVGGALITNDPELDAAFGFLQNGAGAVPGPFDA
HHHCCCHHCCCCEEEECHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHCCCCCCCCCCCE
YLTMRGTKTLAVRMERHCDNAEAIAGFLAKHPAVSTVIYPGLPEHPGHAVADKQMRRFGG
EEEECCCCEEEEEEHHCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHCC
MISVRLHGGRDAALDFCSRTKIFTLAESLGGVESLIEHPGAMTHASTAGSQLEVPADLVR
EEEEEEECCCHHHHHHHHCCHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCEECCHHHHH
LSVGIEDAADLLADVEQALAK
HHCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]