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The map label for this gene is ispH

Identifier: 54026769

GI number: 54026769

Start: 5007355

End: 5008365

Strand: Reverse

Name: ispH

Synonym: nfa47950

Alternate gene names: 54026769

Gene position: 5008365-5007355 (Counterclockwise)

Preceding gene: 54026773

Following gene: 54026767

Centisome position: 83.18

GC content: 70.62

Gene sequence:

>1011_bases
ATGTCTTCGGCCATCCCCTTGAACGTCGGAATCGCCCGCTCCGCGGGCACCGCGACAGTCGACGCCGACACCGCCAAGCG
CGTGCTGCTCGCCGAGCCACGCGGCTACTGCGCGGGCGTCGACCGCGCCGTCGAGACCGTGGAGAAGGCGCTGGAGAAGC
ACGGCGCGCCGATCTACGTGCGCAAGGAGATCGTGCACAACCGGCACGTGGTGGAGACCCTGCGCGAGCGCGGCGTCGTC
TTCGTCGACGACACCGCCGAGGTGCCCGAGGGCGCGGTCGTCGTGTTCTCCGCGCACGGCGTCTCACCCGCGGTGCACGA
GGCCGCCGCCGCCCGCAACCTGCACACCATCGACGCGACCTGTCCGCTGGTCACCAAGGTGCACCAGGAGGCCAAGCGCT
TCGCCCGCGACGACTACGACATCCTGCTGATCGGCCACGAAGGCCACGAGGAGGTCGAAGGCACCGCCGGTGAGGCGCCC
GAGCACGTGCAGCTCGTCGACGGACCCGACGCGGTGGACCGCGTGCGGGTGCGCGACGAGAACAAGGTGATCTGGCTGTC
CCAGACCACCCTGAGCGTCGACGAGACGATGGAGACCGTGCAGCGGCTGCGCGAGCGCTTCCCCTCGCTCCAGGACCCGC
CCAGCGACGACATCTGCTACGCCACCCAGAACCGCCAGGTCGCGGTGAAGGCGATGGCCCCGGAATGCGATCTCGTGATC
GTGGTCGGCTCGCGCAACTCCTCGAACTCGGTGCGCCTGGTCGAGGTCGCGCTCAACGCCGGCGCCAAGGCCGCCCACCT
GGTCGACTACGCCCGCGAGGTCGATCCGGCCTGGCTGGAGGGCGTGCGCACCATCGGCATCACCTCGGGCGCCTCGGTGC
CGGAGATCCTGGTGCGCGGCGTGCTGGACATGCTGGCCGAGCACGGATACGCCGACGTGCAGCCGGTCACCACCGCCAAC
GAGACCCTGGTCTTCGCTCTGCCGCGGGAACTGCGCACCACGCGCCGCTGA

Upstream 100 bases:

>100_bases
TGGTCGAAACATGCTCCTAGCCTGCCCGATGGGCTGTGATCTGCAAAGACCGCACCGCCGCCGGACGACCCGGCACCGGA
TGCACACGTAAGCTGTGGGC

Downstream 100 bases:

>100_bases
CCCTGCCCATCCGCTCGAACGGCCGCCCCGCATCCCGCGGGGCGGCCGTCGTCGTCACAGGCTCTCCGGCTTACGCCGCT
GCGGGCGTCCCGGCTCGCGA

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MSSAIPLNVGIARSAGTATVDADTAKRVLLAEPRGYCAGVDRAVETVEKALEKHGAPIYVRKEIVHNRHVVETLRERGVV
FVDDTAEVPEGAVVVFSAHGVSPAVHEAAAARNLHTIDATCPLVTKVHQEAKRFARDDYDILLIGHEGHEEVEGTAGEAP
EHVQLVDGPDAVDRVRVRDENKVIWLSQTTLSVDETMETVQRLRERFPSLQDPPSDDICYATQNRQVAVKAMAPECDLVI
VVGSRNSSNSVRLVEVALNAGAKAAHLVDYAREVDPAWLEGVRTIGITSGASVPEILVRGVLDMLAEHGYADVQPVTTAN
ETLVFALPRELRTTRR

Sequences:

>Translated_336_residues
MSSAIPLNVGIARSAGTATVDADTAKRVLLAEPRGYCAGVDRAVETVEKALEKHGAPIYVRKEIVHNRHVVETLRERGVV
FVDDTAEVPEGAVVVFSAHGVSPAVHEAAAARNLHTIDATCPLVTKVHQEAKRFARDDYDILLIGHEGHEEVEGTAGEAP
EHVQLVDGPDAVDRVRVRDENKVIWLSQTTLSVDETMETVQRLRERFPSLQDPPSDDICYATQNRQVAVKAMAPECDLVI
VVGSRNSSNSVRLVEVALNAGAKAAHLVDYAREVDPAWLEGVRTIGITSGASVPEILVRGVLDMLAEHGYADVQPVTTAN
ETLVFALPRELRTTRR
>Mature_335_residues
SSAIPLNVGIARSAGTATVDADTAKRVLLAEPRGYCAGVDRAVETVEKALEKHGAPIYVRKEIVHNRHVVETLRERGVVF
VDDTAEVPEGAVVVFSAHGVSPAVHEAAAARNLHTIDATCPLVTKVHQEAKRFARDDYDILLIGHEGHEEVEGTAGEAPE
HVQLVDGPDAVDRVRVRDENKVIWLSQTTLSVDETMETVQRLRERFPSLQDPPSDDICYATQNRQVAVKAMAPECDLVIV
VGSRNSSNSVRLVEVALNAGAKAAHLVDYAREVDPAWLEGVRTIGITSGASVPEILVRGVLDMLAEHGYADVQPVTTANE
TLVFALPRELRTTRR

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=313, Percent_Identity=51.4376996805112, Blast_Score=323, Evalue=8e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_NOCFA (Q5YQ94)

Other databases:

- EMBL:   AP006618
- RefSeq:   YP_121011.1
- GeneID:   3111229
- GenomeReviews:   AP006618_GR
- KEGG:   nfa:nfa47950
- NMPDR:   fig|247156.1.peg.4609
- HOGENOM:   HBG335228
- OMA:   HMQVARA
- ProtClustDB:   PRK01045
- BioCyc:   NFAR247156:NFA47950-MONOMER
- BRENDA:   1.17.1.2
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 36487; Mature: 36355

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSAIPLNVGIARSAGTATVDADTAKRVLLAEPRGYCAGVDRAVETVEKALEKHGAPIYV
CCCCCEEECEEECCCCCEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEE
RKEIVHNRHVVETLRERGVVFVDDTAEVPEGAVVVFSAHGVSPAVHEAAAARNLHTIDAT
EHHHHHCHHHHHHHHHCCCEEEECCCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEECCC
CPLVTKVHQEAKRFARDDYDILLIGHEGHEEVEGTAGEAPEHVQLVDGPDAVDRVRVRDE
CHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHCCCCCCCCCEEEEECCCCCHHHEEEECC
NKVIWLSQTTLSVDETMETVQRLRERFPSLQDPPSDDICYATQNRQVAVKAMAPECDLVI
CEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEEEECCCCCEEE
VVGSRNSSNSVRLVEVALNAGAKAAHLVDYAREVDPAWLEGVRTIGITSGASVPEILVRG
EECCCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCHHHHCCEEEEEECCCCCHHHHHHHH
VLDMLAEHGYADVQPVTTANETLVFALPRELRTTRR
HHHHHHHCCCCCCCCCCCCCCEEEEEECHHHHHCCC
>Mature Secondary Structure 
SSAIPLNVGIARSAGTATVDADTAKRVLLAEPRGYCAGVDRAVETVEKALEKHGAPIYV
CCCCEEECEEECCCCCEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEE
RKEIVHNRHVVETLRERGVVFVDDTAEVPEGAVVVFSAHGVSPAVHEAAAARNLHTIDAT
EHHHHHCHHHHHHHHHCCCEEEECCCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEECCC
CPLVTKVHQEAKRFARDDYDILLIGHEGHEEVEGTAGEAPEHVQLVDGPDAVDRVRVRDE
CHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHCCCCCCCCCEEEEECCCCCHHHEEEECC
NKVIWLSQTTLSVDETMETVQRLRERFPSLQDPPSDDICYATQNRQVAVKAMAPECDLVI
CEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEEEECCCCCEEE
VVGSRNSSNSVRLVEVALNAGAKAAHLVDYAREVDPAWLEGVRTIGITSGASVPEILVRG
EECCCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCHHHHCCEEEEEECCCCCHHHHHHHH
VLDMLAEHGYADVQPVTTANETLVFALPRELRTTRR
HHHHHHHCCCCCCCCCCCCCCEEEEEECHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA