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The map label for this gene is maeB [C]

Identifier: 54026682

GI number: 54026682

Start: 4920303

End: 4921499

Strand: Reverse

Name: maeB [C]

Synonym: nfa47090

Alternate gene names: 54026682

Gene position: 4921499-4920303 (Counterclockwise)

Preceding gene: 54026683

Following gene: 54026681

Centisome position: 81.74

GC content: 70.34

Gene sequence:

>1197_bases
GTGTCACCTGTGACTGACGCACCGAATGCCACCGCGAGTTTGTCCGAAATCACCCACGACGAAATTTTCGCCGGACACCT
CGGGGGCAAGCTCTCGGTCGAACTCTCCGCACCGCTGGAGACACAGCGCGACCTGTCCATCGCCTACACCCCCGGCGTCG
CGCAGGTCAGCCGCGCGATCGCGCAGGACGAGGCGCTCGCCAAGCGCTACACCTGGACCGACCGACTGGTCGTCGTCGTC
AGCGACGGCACCGCGGTGCTCGGCCTCGGTGACATCGGCCCGCGCGCCTCGCTGCCGGTGATGGAGGGCAAGGCCGCGCT
GTTCAAGAAGTTCGCCGGGCTGGACTCCATCCCGATCGTGCTGGACACCAAGGACGTCGACGAGATCGTCGAGACCCTGA
TCCGGCTGCGCCCCAGCTTCGGTGCGGTGAACCTGGAGGACATCTCCGCGCCGCGCTGCTTCGAGATCGAGAAGCGGGTC
ATCGAAGCCCTCGACTGCCCGGTCATGCACGACGACCAGCACGGCACCGCGATCGTGGTGCTGGCCGCCCTCAACGGCGC
GGCCAAGGTGCAGGGCCGCAGCATCGAGGGCCTCAAGGTCGTGGTGTCGGGCGCGGGCGCGGCGGGTGTCGCGTGCACCA
ACATCCTGCTGGCCGCCGGTGTCGCCGACGTGACGGTGCTGGACTCCAAGGGCATCCTCAGCCGCGAGCGTAGTGACCTC
AACGCGGTCAAGGCCGAGCTGGCCGCGCGCACCAACCCGCGCGGGCTGGTCGGCGGCGCCGCCGAGGCACTCGCGGGCGC
GGACGTGTTCCTCGGCCTGTCGGCGGGCCTGATCGCCGAGGAGCTGATCGCTTCGATGGCGCCGGAGTCGATCGTCTTCG
CGATGTCCAACCCGGACCCGGAGATCCACCCCGAGGTGGCGCGCAAGTACGCGGCCATCGTGGCCACCGGGCGCAGCGAC
TTCCCGAACCAGATCAACAACGTGCTCGCGTTCCCCGGCGTGTTCAAGGGCGCGCTCGACGCGGGCGCGCGCCGCATCAC
CGAGGGCATGAAGATCGCCGCCGCGGAGGCCATCCTCGGTGTGGTCGCCGACGAGCTCGGCCCGGACAAGATCGTGCCCA
GCCCGCTCGACCCGCGGGTCGCCCCGGCCGTGGCCGAGGCCGTCGCGGCCGCCGCTCGTGCGGAAGGCGTTGCGTAA

Upstream 100 bases:

>100_bases
TCCCGGTCGGCGCGCCCGATGAGCGGTAACAAGCATCACAAATCAACTACCCGGCGGTAACCGTCCCCCGGTTGAGCCCG
ACCCGAGCAGGAGTGAAATC

Downstream 100 bases:

>100_bases
CAGCTGAATACGCACGAAACGAGGCGCCCCGCTCGATGGTGAGCGGGGCGCCTCGGTGCTGTCCGGGCCGGGCGGCGGTA
GATTCTGCACGAGCCCGTCC

Product: putative malate dehydrogenase

Products: NA

Alternate protein names: NAD-ME [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MSPVTDAPNATASLSEITHDEIFAGHLGGKLSVELSAPLETQRDLSIAYTPGVAQVSRAIAQDEALAKRYTWTDRLVVVV
SDGTAVLGLGDIGPRASLPVMEGKAALFKKFAGLDSIPIVLDTKDVDEIVETLIRLRPSFGAVNLEDISAPRCFEIEKRV
IEALDCPVMHDDQHGTAIVVLAALNGAAKVQGRSIEGLKVVVSGAGAAGVACTNILLAAGVADVTVLDSKGILSRERSDL
NAVKAELAARTNPRGLVGGAAEALAGADVFLGLSAGLIAEELIASMAPESIVFAMSNPDPEIHPEVARKYAAIVATGRSD
FPNQINNVLAFPGVFKGALDAGARRITEGMKIAAAEAILGVVADELGPDKIVPSPLDPRVAPAVAEAVAAAARAEGVA

Sequences:

>Translated_398_residues
MSPVTDAPNATASLSEITHDEIFAGHLGGKLSVELSAPLETQRDLSIAYTPGVAQVSRAIAQDEALAKRYTWTDRLVVVV
SDGTAVLGLGDIGPRASLPVMEGKAALFKKFAGLDSIPIVLDTKDVDEIVETLIRLRPSFGAVNLEDISAPRCFEIEKRV
IEALDCPVMHDDQHGTAIVVLAALNGAAKVQGRSIEGLKVVVSGAGAAGVACTNILLAAGVADVTVLDSKGILSRERSDL
NAVKAELAARTNPRGLVGGAAEALAGADVFLGLSAGLIAEELIASMAPESIVFAMSNPDPEIHPEVARKYAAIVATGRSD
FPNQINNVLAFPGVFKGALDAGARRITEGMKIAAAEAILGVVADELGPDKIVPSPLDPRVAPAVAEAVAAAARAEGVA
>Mature_397_residues
SPVTDAPNATASLSEITHDEIFAGHLGGKLSVELSAPLETQRDLSIAYTPGVAQVSRAIAQDEALAKRYTWTDRLVVVVS
DGTAVLGLGDIGPRASLPVMEGKAALFKKFAGLDSIPIVLDTKDVDEIVETLIRLRPSFGAVNLEDISAPRCFEIEKRVI
EALDCPVMHDDQHGTAIVVLAALNGAAKVQGRSIEGLKVVVSGAGAAGVACTNILLAAGVADVTVLDSKGILSRERSDLN
AVKAELAARTNPRGLVGGAAEALAGADVFLGLSAGLIAEELIASMAPESIVFAMSNPDPEIHPEVARKYAAIVATGRSDF
PNQINNVLAFPGVFKGALDAGARRITEGMKIAAAEAILGVVADELGPDKIVPSPLDPRVAPAVAEAVAAAARAEGVA

Specific function: In addition to the NAD-dependent oxidative decarboxylation of L-malate, the enzyme catalyzes the decarboxylation of oxaloacetate [H]

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=393, Percent_Identity=24.9363867684478, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI62420882, Length=361, Percent_Identity=25.207756232687, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI62420880, Length=361, Percent_Identity=25.207756232687, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI239049447, Length=361, Percent_Identity=25.207756232687, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI4505145, Length=363, Percent_Identity=24.2424242424242, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1788806, Length=391, Percent_Identity=45.2685421994885, Blast_Score=319, Evalue=2e-88,
Organism=Escherichia coli, GI87081919, Length=392, Percent_Identity=26.0204081632653, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17537199, Length=401, Percent_Identity=22.6932668329177, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322823, Length=347, Percent_Identity=25.0720461095101, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24646386, Length=405, Percent_Identity=27.4074074074074, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24646388, Length=405, Percent_Identity=27.4074074074074, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21356279, Length=348, Percent_Identity=25.5747126436782, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI281362674, Length=348, Percent_Identity=25.5747126436782, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI281362672, Length=348, Percent_Identity=25.5747126436782, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI281363503, Length=402, Percent_Identity=23.6318407960199, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI281363505, Length=402, Percent_Identity=23.6318407960199, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI78707236, Length=402, Percent_Identity=23.6318407960199, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI78707232, Length=402, Percent_Identity=23.6318407960199, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI78707240, Length=356, Percent_Identity=24.1573033707865, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI78707242, Length=356, Percent_Identity=24.1573033707865, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI78707238, Length=356, Percent_Identity=24.1573033707865, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 40975; Mature: 40844

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPVTDAPNATASLSEITHDEIFAGHLGGKLSVELSAPLETQRDLSIAYTPGVAQVSRAI
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEECCCHHHHHHHH
AQDEALAKRYTWTDRLVVVVSDGTAVLGLGDIGPRASLPVMEGKAALFKKFAGLDSIPIV
HHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCEEE
LDTKDVDEIVETLIRLRPSFGAVNLEDISAPRCFEIEKRVIEALDCPVMHDDQHGTAIVV
EECCCHHHHHHHHHHHCCCCCCEEHHHCCCCCHHHHHHHHHHHHCCCCEECCCCCCEEEE
LAALNGAAKVQGRSIEGLKVVVSGAGAAGVACTNILLAAGVADVTVLDSKGILSRERSDL
EEECCCCCEECCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHH
NAVKAELAARTNPRGLVGGAAEALAGADVFLGLSAGLIAEELIASMAPESIVFAMSNPDP
HHHHHHHHHCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
EIHPEVARKYAAIVATGRSDFPNQINNVLAFPGVFKGALDAGARRITEGMKIAAAEAILG
CCCHHHHHHHHHHEECCCCCCCHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVADELGPDKIVPSPLDPRVAPAVAEAVAAAARAEGVA
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SPVTDAPNATASLSEITHDEIFAGHLGGKLSVELSAPLETQRDLSIAYTPGVAQVSRAI
CCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEECCCHHHHHHHH
AQDEALAKRYTWTDRLVVVVSDGTAVLGLGDIGPRASLPVMEGKAALFKKFAGLDSIPIV
HHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCEEE
LDTKDVDEIVETLIRLRPSFGAVNLEDISAPRCFEIEKRVIEALDCPVMHDDQHGTAIVV
EECCCHHHHHHHHHHHCCCCCCEEHHHCCCCCHHHHHHHHHHHHCCCCEECCCCCCEEEE
LAALNGAAKVQGRSIEGLKVVVSGAGAAGVACTNILLAAGVADVTVLDSKGILSRERSDL
EEECCCCCEECCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHH
NAVKAELAARTNPRGLVGGAAEALAGADVFLGLSAGLIAEELIASMAPESIVFAMSNPDP
HHHHHHHHHCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
EIHPEVARKYAAIVATGRSDFPNQINNVLAFPGVFKGALDAGARRITEGMKIAAAEAILG
CCCHHHHHHHHHHEECCCCCCCHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVADELGPDKIVPSPLDPRVAPAVAEAVAAAARAEGVA
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2644282 [H]