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The map label for this gene is rpoE [H]

Identifier: 54026557

GI number: 54026557

Start: 4766855

End: 4767508

Strand: Reverse

Name: rpoE [H]

Synonym: nfa45840

Alternate gene names: 54026557

Gene position: 4767508-4766855 (Counterclockwise)

Preceding gene: 54026560

Following gene: 54026556

Centisome position: 79.18

GC content: 67.58

Gene sequence:

>654_bases
GTGACGAGCGACGATGACGTGGTGTCCGACGCCGGCACGACGGACGCCGAGACGGCCGGCACCCCCGTCGACGAAGCCGA
GCTGGCGCAGCGGTTCGAGCGTGACGCGCTCCCGCTGCTCGACCAGCTCTACGGCGCGGCGCTGCGCATGACCAGGAACC
CGGCCGACGCCGAGGACCTCGTCCAGGAGACCTACGTCAAGGCGTTCCAGGGCTTCAAGTCCTTCAAGGAGGGCACCAAC
CTGCGTGCCTGGCTCTACCGGATCCTGACCAACACCTACATCAACTCCTACCGCAAGAAGCAGCGCCAGCCCGCCCAGTA
CCCGACCGACGAGATCACCGACTGGCAGCTGGCCGCGACCGCCGAGCACACCTCCACCGGCCTGCGCTCGGCCGAGGTGG
AGGCGCTGGACGCGCTGCCCGACGACGACATCAAGGCGGCGCTGCAGGAACTGCCCGAAGAGTTCCGGATGGCGGTGTAC
TACGCCGACGTGGAAGGTTTCCCCTACAAGGAGATCGCCGAGATCATGGGCACGCCCATCGGTACGGTGATGTCCCGGCT
GCACCGCGGCCGCAAGCAACTCAAGGGTTTGCTCGCCGACGTGGCGCGCGAACGGGGGTTCAACCGCAGTGAGCGCCAGG
AGGTCAAGCAGTGA

Upstream 100 bases:

>100_bases
GCGCCCGCTGTGGACCGCGGTGTCACCGACGGACGCCGGGTTCCCGCTCGATGGTGACTTGCCGGTAGATTTGGCAAGGA
GGCCGATCGAAGGGACAGGT

Downstream 100 bases:

>100_bases
GTGTCGAGCGCGACCCCGATATGGAGCTGGACTGCACGGCCGTGCTGGCCGACGTGTGGCTGATGCTCGACGGCGAATGC
GACGACGCCACCCGGGCCCG

Product: RNA polymerase sigma factor RpoE

Products: NA

Alternate protein names: Sigma-24 [H]

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MTSDDDVVSDAGTTDAETAGTPVDEAELAQRFERDALPLLDQLYGAALRMTRNPADAEDLVQETYVKAFQGFKSFKEGTN
LRAWLYRILTNTYINSYRKKQRQPAQYPTDEITDWQLAATAEHTSTGLRSAEVEALDALPDDDIKAALQELPEEFRMAVY
YADVEGFPYKEIAEIMGTPIGTVMSRLHRGRKQLKGLLADVARERGFNRSERQEVKQ

Sequences:

>Translated_217_residues
MTSDDDVVSDAGTTDAETAGTPVDEAELAQRFERDALPLLDQLYGAALRMTRNPADAEDLVQETYVKAFQGFKSFKEGTN
LRAWLYRILTNTYINSYRKKQRQPAQYPTDEITDWQLAATAEHTSTGLRSAEVEALDALPDDDIKAALQELPEEFRMAVY
YADVEGFPYKEIAEIMGTPIGTVMSRLHRGRKQLKGLLADVARERGFNRSERQEVKQ
>Mature_216_residues
TSDDDVVSDAGTTDAETAGTPVDEAELAQRFERDALPLLDQLYGAALRMTRNPADAEDLVQETYVKAFQGFKSFKEGTNL
RAWLYRILTNTYINSYRKKQRQPAQYPTDEITDWQLAATAEHTSTGLRSAEVEALDALPDDDIKAALQELPEEFRMAVYY
ADVEGFPYKEIAEIMGTPIGTVMSRLHRGRKQLKGLLADVARERGFNRSERQEVKQ

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=145, Percent_Identity=32.4137931034483, Blast_Score=81, Evalue=5e-17,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014293
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 24487; Mature: 24355

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSDDDVVSDAGTTDAETAGTPVDEAELAQRFERDALPLLDQLYGAALRMTRNPADAEDL
CCCCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
VQETYVKAFQGFKSFKEGTNLRAWLYRILTNTYINSYRKKQRQPAQYPTDEITDWQLAAT
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
AEHTSTGLRSAEVEALDALPDDDIKAALQELPEEFRMAVYYADVEGFPYKEIAEIMGTPI
HHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHCCCH
GTVMSRLHRGRKQLKGLLADVARERGFNRSERQEVKQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCC
>Mature Secondary Structure 
TSDDDVVSDAGTTDAETAGTPVDEAELAQRFERDALPLLDQLYGAALRMTRNPADAEDL
CCCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
VQETYVKAFQGFKSFKEGTNLRAWLYRILTNTYINSYRKKQRQPAQYPTDEITDWQLAAT
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
AEHTSTGLRSAEVEALDALPDDDIKAALQELPEEFRMAVYYADVEGFPYKEIAEIMGTPI
HHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHCCCH
GTVMSRLHRGRKQLKGLLADVARERGFNRSERQEVKQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]