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The map label for this gene is ispB [C]

Identifier: 54026371

GI number: 54026371

Start: 4560086

End: 4561195

Strand: Reverse

Name: ispB [C]

Synonym: nfa44000

Alternate gene names: 54026371

Gene position: 4561195-4560086 (Counterclockwise)

Preceding gene: 54026372

Following gene: 54026370

Centisome position: 75.75

GC content: 75.41

Gene sequence:

>1110_bases
ATGGCGGGCGAGACGATGATCGACCGGCCGGTGCATACCGAGGCGCCTCCGGTGCCCGAGCATCCGGCCGCGTGGTGCAC
CCGGATGCGCGACGACACCGCGCGCGTGCTCGACGATTTCGTGCGCGCGCGCCGCCGCGAGCACCTGGGCGCGCCCGAAA
CCGCCGTGCTGGGGCAGGTGATCGGCGACTTCGTCGCGGGCGGCAAGTACCTGCGTTCGGCGCTGCACCTGGCGGGCTGG
TCGACCGTGGCGCCGGTCGGCGCGGCCGCGATCCGCGCGGCGGCGAGCGCGGAACTGCTGCACTGCTTCGCGCTCATGCA
GGACGACGTGATGGACGAGTCCGCGCGGCGGCGCGGCCGGCCCGCAGCGCACGTCCGGTTCGCGCAGTGGCATGCGCGGC
AAGGATTTTCGGGATCGTCGGACCGCTTCGGCGAGTCCGCGGCGACGCTGGCGGCCGACCTGTGCCTGGTGTGGGCCGAG
CAGCTGCTGCGCGAGAGCGGGGTGGACGCCGCCGCACTGGCCAGGGCGTTGCCCCGCTACGACGCCATGCGTAGCGAACT
GGCGGTCGGGCAGTTCCGCGACCTGGTGAACGACGCGCGGCGGCGGCCGGCACTGGCCGATGTGCTGGCGGTGGCGCGGG
CGAAGTCGGGGCACTACACCGTGCGCCGACCGCTGGAGCTGGGGGCGGAGCTGGCCGGGGCACCGCCGGCGGTGCTCGCG
GCGCTCGGCCGGTACGGGGTCGCCATCGGGGAGGCGTTCCAGGTGCGCGACGACCTGCTCGGCGTCTTCGGCGATCCGGG
CGCGACCGGCAAGCCGGTCGGGGCGGACCTGCGCAACGGCAAGGCCACCACCGTGGTGGTGCTGGCCCGCGAATACGCCG
ACGGCGTGACGCGGCGGGAGCTGGCACGGCTGGCCGCACTGCCGGAACTCGGTGCCGAGGAGATCGCCCGGTACGTGCGG
GTGATCGCGGAGACCGGCGCGCCACGGCGGCTCGAACTGCTGATCGCGCACCGGCTGCGCGAAGGGCTCGCCGCCATCGC
CGGGCTCGAGCCGGGCGCCCGCGCGGTGCTCACCCATCTCGCCCACGCGTGCACCGACCGGTCGCGCTGA

Upstream 100 bases:

>100_bases
CGCGCTGCCGCGGTAACCCGGGCAGCCGTCGGGGCGGTCGGCCGGGTCCGGCCGCCTCGGCGTTTTCGATGCGAGACCGA
TGCGAGAACTGGAGGCCGAC

Downstream 100 bases:

>100_bases
CATCTCGGAGGAGGAATCGATGCGCACCGTGCGCGGGAGAACAGATCGCGTGGTCGTGATCGGGGCAGGGCTGGCGGGGC
TGTCGGCGGCACTGCACCTG

Product: putative polyprenyl synthase

Products: NA

Alternate protein names: GGPP synthase; GGPPSase; (2E,6E)-farnesyl diphosphate synthase; Dimethylallyltranstransferase; Farnesyl diphosphate synthase; Farnesyltranstransferase; Geranylgeranyl diphosphate synthase; Geranyltranstransferase [H]

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MAGETMIDRPVHTEAPPVPEHPAAWCTRMRDDTARVLDDFVRARRREHLGAPETAVLGQVIGDFVAGGKYLRSALHLAGW
STVAPVGAAAIRAAASAELLHCFALMQDDVMDESARRRGRPAAHVRFAQWHARQGFSGSSDRFGESAATLAADLCLVWAE
QLLRESGVDAAALARALPRYDAMRSELAVGQFRDLVNDARRRPALADVLAVARAKSGHYTVRRPLELGAELAGAPPAVLA
ALGRYGVAIGEAFQVRDDLLGVFGDPGATGKPVGADLRNGKATTVVVLAREYADGVTRRELARLAALPELGAEEIARYVR
VIAETGAPRRLELLIAHRLREGLAAIAGLEPGARAVLTHLAHACTDRSR

Sequences:

>Translated_369_residues
MAGETMIDRPVHTEAPPVPEHPAAWCTRMRDDTARVLDDFVRARRREHLGAPETAVLGQVIGDFVAGGKYLRSALHLAGW
STVAPVGAAAIRAAASAELLHCFALMQDDVMDESARRRGRPAAHVRFAQWHARQGFSGSSDRFGESAATLAADLCLVWAE
QLLRESGVDAAALARALPRYDAMRSELAVGQFRDLVNDARRRPALADVLAVARAKSGHYTVRRPLELGAELAGAPPAVLA
ALGRYGVAIGEAFQVRDDLLGVFGDPGATGKPVGADLRNGKATTVVVLAREYADGVTRRELARLAALPELGAEEIARYVR
VIAETGAPRRLELLIAHRLREGLAAIAGLEPGARAVLTHLAHACTDRSR
>Mature_368_residues
AGETMIDRPVHTEAPPVPEHPAAWCTRMRDDTARVLDDFVRARRREHLGAPETAVLGQVIGDFVAGGKYLRSALHLAGWS
TVAPVGAAAIRAAASAELLHCFALMQDDVMDESARRRGRPAAHVRFAQWHARQGFSGSSDRFGESAATLAADLCLVWAEQ
LLRESGVDAAALARALPRYDAMRSELAVGQFRDLVNDARRRPALADVLAVARAKSGHYTVRRPLELGAELAGAPPAVLAA
LGRYGVAIGEAFQVRDDLLGVFGDPGATGKPVGADLRNGKATTVVVLAREYADGVTRRELARLAALPELGAEEIARYVRV
IAETGAPRRLELLIAHRLREGLAAIAGLEPGARAVLTHLAHACTDRSR

Specific function: Catalyzes the trans-addition of the three molecules of IPP onto DMAPP to form geranylgeranyl pyrophosphate which is a precursor of the ether-linked lipids [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1789578, Length=221, Percent_Identity=29.4117647058824, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786623, Length=247, Percent_Identity=32.3886639676113, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17508563, Length=328, Percent_Identity=25.3048780487805, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.1; =2.5.1.29; =2.5.1.10 [H]

Molecular weight: Translated: 39488; Mature: 39356

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGETMIDRPVHTEAPPVPEHPAAWCTRMRDDTARVLDDFVRARRREHLGAPETAVLGQV
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
IGDFVAGGKYLRSALHLAGWSTVAPVGAAAIRAAASAELLHCFALMQDDVMDESARRRGR
HHHHHHCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PAAHVRFAQWHARQGFSGSSDRFGESAATLAADLCLVWAEQLLRESGVDAAALARALPRY
CHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
DAMRSELAVGQFRDLVNDARRRPALADVLAVARAKSGHYTVRRPLELGAELAGAPPAVLA
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEECHHHHCHHHCCCCHHHHH
ALGRYGVAIGEAFQVRDDLLGVFGDPGATGKPVGADLRNGKATTVVVLAREYADGVTRRE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHCCHHHHH
LARLAALPELGAEEIARYVRVIAETGAPRRLELLIAHRLREGLAAIAGLEPGARAVLTHL
HHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
AHACTDRSR
HHHHHCCCC
>Mature Secondary Structure 
AGETMIDRPVHTEAPPVPEHPAAWCTRMRDDTARVLDDFVRARRREHLGAPETAVLGQV
CCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
IGDFVAGGKYLRSALHLAGWSTVAPVGAAAIRAAASAELLHCFALMQDDVMDESARRRGR
HHHHHHCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PAAHVRFAQWHARQGFSGSSDRFGESAATLAADLCLVWAEQLLRESGVDAAALARALPRY
CHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
DAMRSELAVGQFRDLVNDARRRPALADVLAVARAKSGHYTVRRPLELGAELAGAPPAVLA
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEECHHHHCHHHCCCCHHHHH
ALGRYGVAIGEAFQVRDDLLGVFGDPGATGKPVGADLRNGKATTVVVLAREYADGVTRRE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHCCHHHHH
LARLAALPELGAEEIARYVRVIAETGAPRRLELLIAHRLREGLAAIAGLEPGARAVLTHL
HHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
AHACTDRSR
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]