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The map label for this gene is trmD

Identifier: 54026125

GI number: 54026125

Start: 4302656

End: 4303351

Strand: Reverse

Name: trmD

Synonym: nfa41540

Alternate gene names: 54026125

Gene position: 4303351-4302656 (Counterclockwise)

Preceding gene: 54026126

Following gene: 54026122

Centisome position: 71.47

GC content: 70.83

Gene sequence:

>696_bases
ATGAAACTCGACGTCGTCACCATCTTCCCCGAGTATCTGGAGCCGTTGCGCACGGCGCTGCTGGGCAAGGCCATCGACAA
GGGACTGATCTCCGTCGACGTGCACGACCTGCGCCGCTGGACGCACGACGTGCACAAGTCCGTCGACGACGCCCCCTACG
GCGGCGGACCCGGCATGGTCATGAAGCCGACCGTGTGGGGCGACGCGCTCGACGCGGTGTGTCCCGACGACGCGCTGCTC
GTCGTCCCCACCCCGGCCGGCGTGCCCTTCACCCAGGCCACCGCGCAGCGCTGGGCCGCCGAACGCCACCTGGTGTTCGC
GTGCGGTCGCTACGAGGGCATCGACCAGCGTGTCTTCGACGACGCCGCCCGGCGGGTGCGCGTCGAGGAGGTCAGCATCG
GCGACTACGTGCTGATCGGTGGCGAGGCGGCGGTGCTGGTGATGGTCGAGGCGGTCGTCCGGCTGCTGCCCGGGGTGCTC
GGGAATCAGCAGTCCCACGAACAGGATTCGTTCTCCGACGGCCTGCTCGAGGGGCCCAGCTACACCCGCCCGGTGAGCTG
GCGCGGCCTCGAGGTGCCGCCGATCCTGCTCTCCGGTGACCACGCCAAGGTCGCGGCGTGGCGGCGCGAGCAGTCGCTGG
CCCGCACCAGGGAACGCCGTCCCGACCTGTTGCCGCCGGAGTCGTCGTCGGACTGA

Upstream 100 bases:

>100_bases
CCGTTCGTCACCGCCATCGTGCCGACCGTCTCGGTCGCCGAGGGGTACGTCGTGATCGATCCGCCCGAGGGCCTGCTCGA
TCCGGAATGACACGGGCCGC

Downstream 100 bases:

>100_bases
CCGACCTCACTCGGCCGGAACCACCGCCACGGTGCCGTCCGCACCCGGCACCAGGAGTCCCCACGGCGCCATGGTGGGGT
TGTCGCGGCGGGCGAGTGCC

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MKLDVVTIFPEYLEPLRTALLGKAIDKGLISVDVHDLRRWTHDVHKSVDDAPYGGGPGMVMKPTVWGDALDAVCPDDALL
VVPTPAGVPFTQATAQRWAAERHLVFACGRYEGIDQRVFDDAARRVRVEEVSIGDYVLIGGEAAVLVMVEAVVRLLPGVL
GNQQSHEQDSFSDGLLEGPSYTRPVSWRGLEVPPILLSGDHAKVAAWRREQSLARTRERRPDLLPPESSSD

Sequences:

>Translated_231_residues
MKLDVVTIFPEYLEPLRTALLGKAIDKGLISVDVHDLRRWTHDVHKSVDDAPYGGGPGMVMKPTVWGDALDAVCPDDALL
VVPTPAGVPFTQATAQRWAAERHLVFACGRYEGIDQRVFDDAARRVRVEEVSIGDYVLIGGEAAVLVMVEAVVRLLPGVL
GNQQSHEQDSFSDGLLEGPSYTRPVSWRGLEVPPILLSGDHAKVAAWRREQSLARTRERRPDLLPPESSSD
>Mature_231_residues
MKLDVVTIFPEYLEPLRTALLGKAIDKGLISVDVHDLRRWTHDVHKSVDDAPYGGGPGMVMKPTVWGDALDAVCPDDALL
VVPTPAGVPFTQATAQRWAAERHLVFACGRYEGIDQRVFDDAARRVRVEEVSIGDYVLIGGEAAVLVMVEAVVRLLPGVL
GNQQSHEQDSFSDGLLEGPSYTRPVSWRGLEVPPILLSGDHAKVAAWRREQSLARTRERRPDLLPPESSSD

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=228, Percent_Identity=43.421052631579, Blast_Score=183, Evalue=7e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_NOCFA (Q5YS38)

Other databases:

- EMBL:   AP006618
- RefSeq:   YP_120367.1
- ProteinModelPortal:   Q5YS38
- SMR:   Q5YS38
- GeneID:   3111605
- GenomeReviews:   AP006618_GR
- KEGG:   nfa:nfa41540
- NMPDR:   fig|247156.1.peg.4008
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- ProtClustDB:   PRK00026
- BioCyc:   NFAR247156:NFA41540-MONOMER
- BRENDA:   2.1.1.31
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 25366; Mature: 25366

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: BINDING 109-109

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLDVVTIFPEYLEPLRTALLGKAIDKGLISVDVHDLRRWTHDVHKSVDDAPYGGGPGMV
CCCEEEEECHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCE
MKPTVWGDALDAVCPDDALLVVPTPAGVPFTQATAQRWAAERHLVFACGRYEGIDQRVFD
ECCCCCCCHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCEEEEEECCCCCCHHHHHH
DAARRVRVEEVSIGDYVLIGGEAAVLVMVEAVVRLLPGVLGNQQSHEQDSFSDGLLEGPS
HHHHHEEHEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
YTRPVSWRGLEVPPILLSGDHAKVAAWRREQSLARTRERRPDLLPPESSSD
CCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKLDVVTIFPEYLEPLRTALLGKAIDKGLISVDVHDLRRWTHDVHKSVDDAPYGGGPGMV
CCCEEEEECHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCE
MKPTVWGDALDAVCPDDALLVVPTPAGVPFTQATAQRWAAERHLVFACGRYEGIDQRVFD
ECCCCCCCHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCEEEEEECCCCCCHHHHHH
DAARRVRVEEVSIGDYVLIGGEAAVLVMVEAVVRLLPGVLGNQQSHEQDSFSDGLLEGPS
HHHHHEEHEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
YTRPVSWRGLEVPPILLSGDHAKVAAWRREQSLARTRERRPDLLPPESSSD
CCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA