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The map label for this gene is ispG

Identifier: 54026089

GI number: 54026089

Start: 4266999

End: 4268156

Strand: Reverse

Name: ispG

Synonym: nfa41180

Alternate gene names: 54026089

Gene position: 4268156-4266999 (Counterclockwise)

Preceding gene: 54026090

Following gene: 54026088

Centisome position: 70.89

GC content: 68.31

Gene sequence:

>1158_bases
GTGACCAGCACCATCGGATTGGGGATGCCGACCGCTCCGGCGGCCGTTCTCGCCCCCCGCCGCAAGACCCGCCAGCTGAT
GGTGGGCTCGGTCGGCGTGGGCAGCGATCACCCGGTCTCCGTGCAGTCCATGACCACCACGAAGACCCACGACGTCAACG
CCACGCTGCAGCAGATCGCGCAGCTCACCGCGTCCGGCTGTGACATCGTGCGGGTGGCGTGCCCGCGTCAGGAAGACGCC
GACGCGCTGGCGACGATCGCGAAGAAGAGCCAGATCCCGGTCATCGCCGACATCCACTTCCAGCCGCGCTACATCTTCGC
CGCGATCGATGCCGGGTGTGCCGCGGTGCGCGTGAATCCCGGCAACATCAAGGAATTCGACGGCCGGGTGAAAGAGGTCG
CCAAGGCGGCGGGCGCCGCGGGTATTCCGATCCGGATCGGCGTGAACGCCGGATCGCTGGACAAGCGGATGCTGGAGAAG
TACGGCAAGGCCACCCCGGAGGCGCTGGTGGAATCGGCGCTGTGGGAGGCGAGCCTGTTCGAGGAGCACGGTTTCGGCGA
CATCAAGATCTCGGTCAAGCACAACGACCCGGTGATCATGGTGGAGGCCTACCGGCAGCTGGCCGCGCAGTGCGATTACC
CGCTGCACCTGGGCGTCACCGAGGCGGGTCCGGCGTTCCAGGGCACCATCAAGTCGGCGGTGGCCTTCGGCGCGCTGCTG
AGCGAGGGCATCGGTGACACCATCCGGGTCTCGCTGTCGGCGCCGCCGGTCGAAGAGGTCAAGGTCGGCACGCAGATCCT
GCAGTCGCTGAACCTGCGCCCGCGCAAGCTCGAGATCGTGTCCTGTCCGTCGTGCGGCCGTGCCCAGGTCGATGTCTACA
CCCTCGCCAACGAGGTGACCGCGGGGCTGGAGGGCATGGAGGTGCCGCTGCGGGTGGCCGTGATGGGGTGCGTGGTGAAC
GGCCCGGGCGAGGCGCGTGAGGCCGATCTGGGCGTGGCCTCCGGCAACGGCAAGGGCCAGATCTTCGTCAAGGGCCAGGT
GATCAAGACCGTGCCCGAGCACCAGATCGTGGAGACGCTGATCGAAGAGGCGATGCGGATCGCCGAGGAGATGGGCGAGC
AGGCCGGCTCCGGCGAGCCGGTCGTCACCGTGAGCTGA

Upstream 100 bases:

>100_bases
GGTGCTGTGGCTTCGACCTCACCTGGTGAGGCGGGCTTCGCCGGTGACTAAGCTCGGAAGCGAACGGCCCGCACGAACGT
AGGAAAGTAGGCAGCCGACA

Downstream 100 bases:

>100_bases
CCTGGTCTTTCCCACGAGTTGTACCGCGTCGGTCCGGTTCGTGGCAGGCTGTCGGTGTGCGGAGTCTGCGGGAGCCGACG
CGTCGGGCGAAATACGTTGC

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MTSTIGLGMPTAPAAVLAPRRKTRQLMVGSVGVGSDHPVSVQSMTTTKTHDVNATLQQIAQLTASGCDIVRVACPRQEDA
DALATIAKKSQIPVIADIHFQPRYIFAAIDAGCAAVRVNPGNIKEFDGRVKEVAKAAGAAGIPIRIGVNAGSLDKRMLEK
YGKATPEALVESALWEASLFEEHGFGDIKISVKHNDPVIMVEAYRQLAAQCDYPLHLGVTEAGPAFQGTIKSAVAFGALL
SEGIGDTIRVSLSAPPVEEVKVGTQILQSLNLRPRKLEIVSCPSCGRAQVDVYTLANEVTAGLEGMEVPLRVAVMGCVVN
GPGEAREADLGVASGNGKGQIFVKGQVIKTVPEHQIVETLIEEAMRIAEEMGEQAGSGEPVVTVS

Sequences:

>Translated_385_residues
MTSTIGLGMPTAPAAVLAPRRKTRQLMVGSVGVGSDHPVSVQSMTTTKTHDVNATLQQIAQLTASGCDIVRVACPRQEDA
DALATIAKKSQIPVIADIHFQPRYIFAAIDAGCAAVRVNPGNIKEFDGRVKEVAKAAGAAGIPIRIGVNAGSLDKRMLEK
YGKATPEALVESALWEASLFEEHGFGDIKISVKHNDPVIMVEAYRQLAAQCDYPLHLGVTEAGPAFQGTIKSAVAFGALL
SEGIGDTIRVSLSAPPVEEVKVGTQILQSLNLRPRKLEIVSCPSCGRAQVDVYTLANEVTAGLEGMEVPLRVAVMGCVVN
GPGEAREADLGVASGNGKGQIFVKGQVIKTVPEHQIVETLIEEAMRIAEEMGEQAGSGEPVVTVS
>Mature_384_residues
TSTIGLGMPTAPAAVLAPRRKTRQLMVGSVGVGSDHPVSVQSMTTTKTHDVNATLQQIAQLTASGCDIVRVACPRQEDAD
ALATIAKKSQIPVIADIHFQPRYIFAAIDAGCAAVRVNPGNIKEFDGRVKEVAKAAGAAGIPIRIGVNAGSLDKRMLEKY
GKATPEALVESALWEASLFEEHGFGDIKISVKHNDPVIMVEAYRQLAAQCDYPLHLGVTEAGPAFQGTIKSAVAFGALLS
EGIGDTIRVSLSAPPVEEVKVGTQILQSLNLRPRKLEIVSCPSCGRAQVDVYTLANEVTAGLEGMEVPLRVAVMGCVVNG
PGEAREADLGVASGNGKGQIFVKGQVIKTVPEHQIVETLIEEAMRIAEEMGEQAGSGEPVVTVS

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=358, Percent_Identity=45.2513966480447, Blast_Score=312, Evalue=3e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_NOCFA (Q5YS74)

Other databases:

- EMBL:   AP006618
- RefSeq:   YP_120331.1
- ProteinModelPortal:   Q5YS74
- GeneID:   3109118
- GenomeReviews:   AP006618_GR
- KEGG:   nfa:nfa41180
- NMPDR:   fig|247156.1.peg.3975
- HOGENOM:   HBG335271
- OMA:   APIVVQS
- ProtClustDB:   PRK00366
- BioCyc:   NFAR247156:NFA41180-MONOMER
- BRENDA:   1.17.4.3
- HAMAP:   MF_00159
- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE; SSF51717 DHP_synth_like

EC number: =1.17.7.1

Molecular weight: Translated: 40476; Mature: 40345

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTIGLGMPTAPAAVLAPRRKTRQLMVGSVGVGSDHPVSVQSMTTTKTHDVNATLQQIA
CCCCCCCCCCCCCHHHHCCCHHHHHEEEEECCCCCCCCEEEEEEECCCCCCCHHHHHHHH
QLTASGCDIVRVACPRQEDADALATIAKKSQIPVIADIHFQPRYIFAAIDAGCAAVRVNP
HHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCEEEEEEECCCCEEEEECC
GNIKEFDGRVKEVAKAAGAAGIPIRIGVNAGSLDKRMLEKYGKATPEALVESALWEASLF
CCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
EEHGFGDIKISVKHNDPVIMVEAYRQLAAQCDYPLHLGVTEAGPAFQGTIKSAVAFGALL
HHCCCCEEEEEEECCCCEEEHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHH
SEGIGDTIRVSLSAPPVEEVKVGTQILQSLNLRPRKLEIVSCPSCGRAQVDVYTLANEVT
HCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEEEHHHHHH
AGLEGMEVPLRVAVMGCVVNGPGEAREADLGVASGNGKGQIFVKGQVIKTVPEHQIVETL
CCCCCCCCCCEEEEEEEEECCCCCCCCCCCEEECCCCCEEEEEECEEEECCCHHHHHHHH
IEEAMRIAEEMGEQAGSGEPVVTVS
HHHHHHHHHHHHHHCCCCCCEEEEC
>Mature Secondary Structure 
TSTIGLGMPTAPAAVLAPRRKTRQLMVGSVGVGSDHPVSVQSMTTTKTHDVNATLQQIA
CCCCCCCCCCCCHHHHCCCHHHHHEEEEECCCCCCCCEEEEEEECCCCCCCHHHHHHHH
QLTASGCDIVRVACPRQEDADALATIAKKSQIPVIADIHFQPRYIFAAIDAGCAAVRVNP
HHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCEEEEEEECCCCEEEEECC
GNIKEFDGRVKEVAKAAGAAGIPIRIGVNAGSLDKRMLEKYGKATPEALVESALWEASLF
CCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
EEHGFGDIKISVKHNDPVIMVEAYRQLAAQCDYPLHLGVTEAGPAFQGTIKSAVAFGALL
HHCCCCEEEEEEECCCCEEEHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHH
SEGIGDTIRVSLSAPPVEEVKVGTQILQSLNLRPRKLEIVSCPSCGRAQVDVYTLANEVT
HCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEEEHHHHHH
AGLEGMEVPLRVAVMGCVVNGPGEAREADLGVASGNGKGQIFVKGQVIKTVPEHQIVETL
CCCCCCCCCCEEEEEEEEECCCCCCCCCCCEEECCCCCEEEEEECEEEECCCHHHHHHHH
IEEAMRIAEEMGEQAGSGEPVVTVS
HHHHHHHHHHHHHHCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA