Click here to switch to the map view.

The map label for this gene is hsaD [H]

Identifier: 54026065

GI number: 54026065

Start: 4245917

End: 4246783

Strand: Reverse

Name: hsaD [H]

Synonym: nfa40940

Alternate gene names: 54026065

Gene position: 4246783-4245917 (Counterclockwise)

Preceding gene: 54026066

Following gene: 54026062

Centisome position: 70.53

GC content: 70.01

Gene sequence:

>867_bases
ATGAGTACTCCAGTCGATACCCTCGCCGTCCCCGAACTGCGCGAACTCACCACCGAACACGGGGTGTTGCGCTACCGCGA
ATGCGGGGACGGGCCGCCGCTGCTGCTGCTGCACGGCTCCGGCCCCGGCGTCACCGGATGGCGCAACTTCGGCGGCAACG
TCCCGGTCTTCGCCGAGCACTACCGGACGCTGGTCCTGGAGTTCCCCGGGTTCGGCGTCAGTGACGATTTCGGCGCCGTC
CACCCGATGCTGTCGGCGCAGCAGGCCGTGCGCGCTTTCCTCGACGGGCTCGGCCTGGAACAGGTGCGCATCATCGGCAA
CTCGATGGGTGGTTTCGTCGCCACCGATTTCGCCATCGCCGCGCCCGAGCGGGTGGAGCGCCTGGTCACCATCGGCGGCA
TCGGGACCCCGTTGTTCAGTGCGCAGCCGGGGGAGGGCATCATCCGGCTCGGCGAGTTCGTCGACAACCCCACCCGGGAA
CGGCTGATCGCGTGGCTGCGGTCGATGGTGTTCGACCAGTCGCTGGTGACCGAGGAACTGATCGAGCAGCGCTGGCAGCA
GGCGACCGACCCGGCCACGCTGGAGAATTCGCGGCGCATGTACGGCAGCGCCGCGCTGGCCCGCATGGCCGAGGCGGCGC
GCGCCGCCGACACCACCCCGGGGTGGGCGAATCTCGGCCGGATCACCGCCCCGGTCCTGGTCACCTGGGGCCGTGACGAC
CGGGTCAGCCCGGTCGACATGGCGCTGCTCCCGATGCGCACGCTCCGCAACGGCGAAGTGCACATCTTCCCCAACTGCGG
GCACTGGGTGATGATCGAGCAGAAGCGCGCCTGGGAGGCCGTGGTGCTGGCCTTCCTCGGCCGCTAG

Upstream 100 bases:

>100_bases
CTCGGCACCCCGCGGTGAATCCGGCGGCACCGGCGGAAAGCTGTGCCGGTCACCGGGAATTCGGCCCGGCGGGGCGCCGC
TCCGGTGGCAGGCTCGGGGC

Downstream 100 bases:

>100_bases
CCGGCCGCCGCGACCGGCGCCGCGTCGACCACCGGCGCCGGTCTCGCGCGAGAGCTGCCGACGCGCGACAGCAGCGGGCG
AGAGCTGTCGACGGCCGCGC

Product: putative hydrolase

Products: NA

Alternate protein names: 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase; HOPDA hydrolase; Meta-cleavage product hydrolase; MCP hydrolase [H]

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MSTPVDTLAVPELRELTTEHGVLRYRECGDGPPLLLLHGSGPGVTGWRNFGGNVPVFAEHYRTLVLEFPGFGVSDDFGAV
HPMLSAQQAVRAFLDGLGLEQVRIIGNSMGGFVATDFAIAAPERVERLVTIGGIGTPLFSAQPGEGIIRLGEFVDNPTRE
RLIAWLRSMVFDQSLVTEELIEQRWQQATDPATLENSRRMYGSAALARMAEAARAADTTPGWANLGRITAPVLVTWGRDD
RVSPVDMALLPMRTLRNGEVHIFPNCGHWVMIEQKRAWEAVVLAFLGR

Sequences:

>Translated_288_residues
MSTPVDTLAVPELRELTTEHGVLRYRECGDGPPLLLLHGSGPGVTGWRNFGGNVPVFAEHYRTLVLEFPGFGVSDDFGAV
HPMLSAQQAVRAFLDGLGLEQVRIIGNSMGGFVATDFAIAAPERVERLVTIGGIGTPLFSAQPGEGIIRLGEFVDNPTRE
RLIAWLRSMVFDQSLVTEELIEQRWQQATDPATLENSRRMYGSAALARMAEAARAADTTPGWANLGRITAPVLVTWGRDD
RVSPVDMALLPMRTLRNGEVHIFPNCGHWVMIEQKRAWEAVVLAFLGR
>Mature_287_residues
STPVDTLAVPELRELTTEHGVLRYRECGDGPPLLLLHGSGPGVTGWRNFGGNVPVFAEHYRTLVLEFPGFGVSDDFGAVH
PMLSAQQAVRAFLDGLGLEQVRIIGNSMGGFVATDFAIAAPERVERLVTIGGIGTPLFSAQPGEGIIRLGEFVDNPTRER
LIAWLRSMVFDQSLVTEELIEQRWQQATDPATLENSRRMYGSAALARMAEAARAADTTPGWANLGRITAPVLVTWGRDDR
VSPVDMALLPMRTLRNGEVHIFPNCGHWVMIEQKRAWEAVVLAFLGR

Specific function: Catalyzes the hydrolysis of a carbon-carbon bond in 4,5- 9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oic acid (4,9-DSHA) to yield 9,17-dioxo-1,2,3,4,10,19- hexanorandrostan-5-oic acid (DOHNAA) and 2-hydroxy-hexa-2,4- dienoic acid (HHD). Is

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. HsaD family [H]

Homologues:

Organism=Escherichia coli, GI87081721, Length=273, Percent_Identity=36.2637362637363, Blast_Score=157, Evalue=7e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.7.1.8 [H]

Molecular weight: Translated: 31571; Mature: 31439

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPVDTLAVPELRELTTEHGVLRYRECGDGPPLLLLHGSGPGVTGWRNFGGNVPVFAEH
CCCCCCCCCCCHHHHHHHHCCCEEEHCCCCCCCEEEEECCCCCCCCHHHCCCCCCCHHHH
YRTLVLEFPGFGVSDDFGAVHPMLSAQQAVRAFLDGLGLEQVRIIGNSMGGFVATDFAIA
HEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHEECCCCCCHHHHHHHHC
APERVERLVTIGGIGTPLFSAQPGEGIIRLGEFVDNPTRERLIAWLRSMVFDQSLVTEEL
CHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
IEQRWQQATDPATLENSRRMYGSAALARMAEAARAADTTPGWANLGRITAPVLVTWGRDD
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCEEECEEEEECCCC
RVSPVDMALLPMRTLRNGEVHIFPNCGHWVMIEQKRAWEAVVLAFLGR
CCCCHHHHHHHHHHHCCCCEEEEECCCCEEEEECHHHHHHHHHHHHCC
>Mature Secondary Structure 
STPVDTLAVPELRELTTEHGVLRYRECGDGPPLLLLHGSGPGVTGWRNFGGNVPVFAEH
CCCCCCCCCCHHHHHHHHCCCEEEHCCCCCCCEEEEECCCCCCCCHHHCCCCCCCHHHH
YRTLVLEFPGFGVSDDFGAVHPMLSAQQAVRAFLDGLGLEQVRIIGNSMGGFVATDFAIA
HEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHEECCCCCCHHHHHHHHC
APERVERLVTIGGIGTPLFSAQPGEGIIRLGEFVDNPTRERLIAWLRSMVFDQSLVTEEL
CHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
IEQRWQQATDPATLENSRRMYGSAALARMAEAARAADTTPGWANLGRITAPVLVTWGRDD
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCEEECEEEEECCCC
RVSPVDMALLPMRTLRNGEVHIFPNCGHWVMIEQKRAWEAVVLAFLGR
CCCCHHHHHHHHHHHCCCCEEEEECCCCEEEEECHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]