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The map label for this gene is dnaN2 [H]

Identifier: 54025936

GI number: 54025936

Start: 4158604

End: 4159749

Strand: Reverse

Name: dnaN2 [H]

Synonym: nfa39660

Alternate gene names: 54025936

Gene position: 4159749-4158604 (Counterclockwise)

Preceding gene: 54025937

Following gene: 54025935

Centisome position: 69.08

GC content: 68.94

Gene sequence:

>1146_bases
ATGAGCATCCGATTCCGCGTCGCCCGCGGCGACCTCGCAGACGCAGCCACCTGGGTAGCGCGTAGCCTCCCGAGCCGCCC
ACCCGTCCCCGTGCTCGGCGGTGCCCACCTGCGCGCCGACGACGACGGCCTCACCATCTCCGTCTTCGACTACGAGGTGT
CCGCGCAGATGCGGATCGCCGCCGAGGTCGCCCACGACGGCGAAGTGCTGGTGTCGGGGAAGCTGCTCGCCGACATCACC
AAGGCGCTGCCGAACAAGCCCGTCGACATCACCTTCGACGGCAGCCGAGTCCTCATCGCCTGCGGCAGCGCGAAGTTCTC
CCTGCCCACTATGCCGGTCGAGGACTACCCGAAGCTGCCCGACGTCCCTGCCCGGAGCGGCGAGCTGGCCGCGGACCTGT
TCGCGACCGCGGTCAGCCAGGTCGCTGTGGCGGCCGGCCGGGACGACACCCTGCCGATGCTGACCGGTATCCGCGTCGAA
ATTGACGGCGCCGAGGTGACGTTGGCGGCGACCGACCGGTACCGGTTGGCGGTGCGTACGCTCGAATGGTCCCCAACCGA
CCCGAGTGTCGAAACCCAGGTTCTGGTTCCCGCCCGGACTCTCGCCGAGGCGGTGAAGGCGCTGGAATCGGCGGCGCCCG
CCCGGTTGGCGATCGACGACAGCATGTTCGGACTGGTGAACGCCCGCCGCCGCACCACCACACGCCTACTGGATGCCGAG
TTCCCGAAGTTCCGGCAGTTGCTGCCGAAGGAGCACACGTCGGTGGCGACTGTGTTCGTCCCGGATCTCGTGGACGCGAT
CAAGCGTGTGGCGCTCGTCGCCGAGCGGGGCGCACAGGTGCGGCTGGAGTTCAGCGAGGACGGATTGCTGCTGTCCGCTG
GCGGCGACGATGCGGGCCGCGCCGAGGAAGCGGTGCCCGCCGAGTTTCGGGGCGAGCCGCTGACGATTGCGTTCAACCCG
GGCTATCTGATGGACGGGTTGACGGCGTTGCATTCGACCAGTGTCACGCTCGGGTTCACGACGCCGACGCGGCCCGCCGT
GCTGGTGCCCAGCGGCGACGTCGAGGTGAAGGAGAGCAGTGGCGGGTTCGCTGCGCTGTCTGGTCCATTCGTATACATGC
TGATGCCCGTGCGGATTCCGGGGTGA

Upstream 100 bases:

>100_bases
ACTGCGCCGCCGACACGCCTGGCTACGAGTGGGAGCCCGACTACGACCACCACCAGAAGAAGCCCACCACCAAAAAGCCC
ACCCCAACCAAGAAGGCCCG

Downstream 100 bases:

>100_bases
CCGGTCCCGCAGTTGGGCCGACACCGTAAGTAGCTTCTCGGTTGCCGTTCATGTAGAAGCCAGCGCGACGCTCGGAACCA
TCCGCTAAGCTCAAACCCCT

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MSIRFRVARGDLADAATWVARSLPSRPPVPVLGGAHLRADDDGLTISVFDYEVSAQMRIAAEVAHDGEVLVSGKLLADIT
KALPNKPVDITFDGSRVLIACGSAKFSLPTMPVEDYPKLPDVPARSGELAADLFATAVSQVAVAAGRDDTLPMLTGIRVE
IDGAEVTLAATDRYRLAVRTLEWSPTDPSVETQVLVPARTLAEAVKALESAAPARLAIDDSMFGLVNARRRTTTRLLDAE
FPKFRQLLPKEHTSVATVFVPDLVDAIKRVALVAERGAQVRLEFSEDGLLLSAGGDDAGRAEEAVPAEFRGEPLTIAFNP
GYLMDGLTALHSTSVTLGFTTPTRPAVLVPSGDVEVKESSGGFAALSGPFVYMLMPVRIPG

Sequences:

>Translated_381_residues
MSIRFRVARGDLADAATWVARSLPSRPPVPVLGGAHLRADDDGLTISVFDYEVSAQMRIAAEVAHDGEVLVSGKLLADIT
KALPNKPVDITFDGSRVLIACGSAKFSLPTMPVEDYPKLPDVPARSGELAADLFATAVSQVAVAAGRDDTLPMLTGIRVE
IDGAEVTLAATDRYRLAVRTLEWSPTDPSVETQVLVPARTLAEAVKALESAAPARLAIDDSMFGLVNARRRTTTRLLDAE
FPKFRQLLPKEHTSVATVFVPDLVDAIKRVALVAERGAQVRLEFSEDGLLLSAGGDDAGRAEEAVPAEFRGEPLTIAFNP
GYLMDGLTALHSTSVTLGFTTPTRPAVLVPSGDVEVKESSGGFAALSGPFVYMLMPVRIPG
>Mature_380_residues
SIRFRVARGDLADAATWVARSLPSRPPVPVLGGAHLRADDDGLTISVFDYEVSAQMRIAAEVAHDGEVLVSGKLLADITK
ALPNKPVDITFDGSRVLIACGSAKFSLPTMPVEDYPKLPDVPARSGELAADLFATAVSQVAVAAGRDDTLPMLTGIRVEI
DGAEVTLAATDRYRLAVRTLEWSPTDPSVETQVLVPARTLAEAVKALESAAPARLAIDDSMFGLVNARRRTTTRLLDAEF
PKFRQLLPKEHTSVATVFVPDLVDAIKRVALVAERGAQVRLEFSEDGLLLSAGGDDAGRAEEAVPAEFRGEPLTIAFNPG
YLMDGLTALHSTSVTLGFTTPTRPAVLVPSGDVEVKESSGGFAALSGPFVYMLMPVRIPG

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=387, Percent_Identity=27.6485788113695, Blast_Score=129, Evalue=2e-31,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40468; Mature: 40336

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIRFRVARGDLADAATWVARSLPSRPPVPVLGGAHLRADDDGLTISVFDYEVSAQMRIA
CCEEEEECCCCHHHHHHHHHHHCCCCCCCCEECCCEEEECCCCCEEEEEEEEECCEEEEE
AEVAHDGEVLVSGKLLADITKALPNKPVDITFDGSRVLIACGSAKFSLPTMPVEDYPKLP
EEECCCCCEEEECHHHHHHHHHCCCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCC
DVPARSGELAADLFATAVSQVAVAAGRDDTLPMLTGIRVEIDGAEVTLAATDRYRLAVRT
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECEEEEECCCEEEEEECCCEEEEEEE
LEWSPTDPSVETQVLVPARTLAEAVKALESAAPARLAIDDSMFGLVNARRRTTTRLLDAE
EECCCCCCCCCEEEEECHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHCCC
FPKFRQLLPKEHTSVATVFVPDLVDAIKRVALVAERGAQVRLEFSEDGLLLSAGGDDAGR
CHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEECCCCCCCC
AEEAVPAEFRGEPLTIAFNPGYLMDGLTALHSTSVTLGFTTPTRPAVLVPSGDVEVKESS
CCCCCCHHHCCCEEEEEECCCHHHHHHHHHHCCEEEEEECCCCCCEEEEECCCEEEEECC
GGFAALSGPFVYMLMPVRIPG
CCEEEECCCEEEEEEEEECCC
>Mature Secondary Structure 
SIRFRVARGDLADAATWVARSLPSRPPVPVLGGAHLRADDDGLTISVFDYEVSAQMRIA
CEEEEECCCCHHHHHHHHHHHCCCCCCCCEECCCEEEECCCCCEEEEEEEEECCEEEEE
AEVAHDGEVLVSGKLLADITKALPNKPVDITFDGSRVLIACGSAKFSLPTMPVEDYPKLP
EEECCCCCEEEECHHHHHHHHHCCCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCC
DVPARSGELAADLFATAVSQVAVAAGRDDTLPMLTGIRVEIDGAEVTLAATDRYRLAVRT
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECEEEEECCCEEEEEECCCEEEEEEE
LEWSPTDPSVETQVLVPARTLAEAVKALESAAPARLAIDDSMFGLVNARRRTTTRLLDAE
EECCCCCCCCCEEEEECHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHCCC
FPKFRQLLPKEHTSVATVFVPDLVDAIKRVALVAERGAQVRLEFSEDGLLLSAGGDDAGR
CHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEECCCCCCCC
AEEAVPAEFRGEPLTIAFNPGYLMDGLTALHSTSVTLGFTTPTRPAVLVPSGDVEVKESS
CCCCCCHHHCCCEEEEEECCCHHHHHHHHHHCCEEEEEECCCCCCEEEEECCCEEEEECC
GGFAALSGPFVYMLMPVRIPG
CCEEEECCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8733228 [H]