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The map label for this gene is dapF [H]

Identifier: 54025799

GI number: 54025799

Start: 4049104

End: 4050012

Strand: Reverse

Name: dapF [H]

Synonym: nfa38290

Alternate gene names: 54025799

Gene position: 4050012-4049104 (Counterclockwise)

Preceding gene: 54025800

Following gene: 54025798

Centisome position: 67.26

GC content: 74.37

Gene sequence:

>909_bases
GTGACGGACATCGGCGGGACGGACATCGAGTTCACGAAGGGCCACGGCACCGAGAACGACTTCGTCGTGCTGCCCGACGA
GGACGTCCGGCTCGACCTGACGCCCGCGCGGGTGGCCGCGCTCTGCGACCGGCAGCGCGGCCTCGGCGCGGACGGGGTGC
TGCGGGTGGCGCGCGCCGGTGCGCTGCTGCGCGCGGGCGTGCTCGACGCGTTGCCCGCCGGGGTGTCCGACACCGACTGG
TTCATGGACTACCGCAACGCCGACGGATCGATCGCGGAGATGTGCGGCAACGGCGTGCGGGTCTTCGCCCACTATCTGGC
CGCCACCGGACGTGCGGAGGGCACCGAGCACGTGGTAGGCAGCCGGGCGGGCGCGCGGCCGGTGACCGTGCACGCGGCAG
GCCCGACCCACGGCGAGGTCACCGTGGCGATGGGGGAGGTCCGTGCGCTGGGCGCCTCCACCGCGACCGTCGCCGGCTGG
GGCTACTCCGGTCTCGGTATCGATGTCGGGAATCCGCACCTGGCGTGCGTGGACCCCACACTCACCGCGGAGGCGCTGGC
CAAGCTGGACCTGACCGTCTCGCCCGGCTACGACCCCGACCTGTTCCCGCACGGGGTGAACGTCGAGATCCTGACCCCGC
TCGACGAGCAGCGCGCGGTGGACATGCGCGTCTACGAGCGCGGCGTCGGCGAAACCCGGTCGTGCGGAACGGGAACCGTG
GCGGCGGCGGCCGCGGCCCTGAGTGCCGAGGGCTTCGAGCTGGCCACCGGCAGCGGGGCGGTCACGGTACGGGTGCCCGG
CGGCGCGGTGCGGGTCGGGCTGGAGGCGGGCTCGGCGTGGCTGCGCGGTCCGTCGGTGCTGGTCGCGACCGGTCGCCTCA
CGGCGGACTGGTGGCAGACGCTCGGATGA

Upstream 100 bases:

>100_bases
GTTCCCTGGGCGCACCGTCGGCGGGTGCGGTCGCCCTCTACGAAATATCCCGGCAGCGGCGGACGTTTGTCCGATGACGA
CGGAAACGGAGACCACAGGC

Downstream 100 bases:

>100_bases
TCACCCGGGCGAAATCGGAATGCACGCCACCCCACCGGCGTGGCAGCATGGATGGTGTATGAGGAAATCAGAGACGTTCG
ACATCACTTCGACCTCCGGC

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MTDIGGTDIEFTKGHGTENDFVVLPDEDVRLDLTPARVAALCDRQRGLGADGVLRVARAGALLRAGVLDALPAGVSDTDW
FMDYRNADGSIAEMCGNGVRVFAHYLAATGRAEGTEHVVGSRAGARPVTVHAAGPTHGEVTVAMGEVRALGASTATVAGW
GYSGLGIDVGNPHLACVDPTLTAEALAKLDLTVSPGYDPDLFPHGVNVEILTPLDEQRAVDMRVYERGVGETRSCGTGTV
AAAAAALSAEGFELATGSGAVTVRVPGGAVRVGLEAGSAWLRGPSVLVATGRLTADWWQTLG

Sequences:

>Translated_302_residues
MTDIGGTDIEFTKGHGTENDFVVLPDEDVRLDLTPARVAALCDRQRGLGADGVLRVARAGALLRAGVLDALPAGVSDTDW
FMDYRNADGSIAEMCGNGVRVFAHYLAATGRAEGTEHVVGSRAGARPVTVHAAGPTHGEVTVAMGEVRALGASTATVAGW
GYSGLGIDVGNPHLACVDPTLTAEALAKLDLTVSPGYDPDLFPHGVNVEILTPLDEQRAVDMRVYERGVGETRSCGTGTV
AAAAAALSAEGFELATGSGAVTVRVPGGAVRVGLEAGSAWLRGPSVLVATGRLTADWWQTLG
>Mature_301_residues
TDIGGTDIEFTKGHGTENDFVVLPDEDVRLDLTPARVAALCDRQRGLGADGVLRVARAGALLRAGVLDALPAGVSDTDWF
MDYRNADGSIAEMCGNGVRVFAHYLAATGRAEGTEHVVGSRAGARPVTVHAAGPTHGEVTVAMGEVRALGASTATVAGWG
YSGLGIDVGNPHLACVDPTLTAEALAKLDLTVSPGYDPDLFPHGVNVEILTPLDEQRAVDMRVYERGVGETRSCGTGTVA
AAAAALSAEGFELATGSGAVTVRVPGGAVRVGLEAGSAWLRGPSVLVATGRLTADWWQTLG

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=299, Percent_Identity=27.7591973244147, Blast_Score=82, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 31027; Mature: 30896

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDIGGTDIEFTKGHGTENDFVVLPDEDVRLDLTPARVAALCDRQRGLGADGVLRVARAG
CCCCCCCCEEEECCCCCCCCEEEECCCCCEEEECHHHHHHHHHHHCCCCHHHHHHHHHHH
ALLRAGVLDALPAGVSDTDWFMDYRNADGSIAEMCGNGVRVFAHYLAATGRAEGTEHVVG
HHHHHHHHHHCCCCCCCCHHEEEECCCCCHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHC
SRAGARPVTVHAAGPTHGEVTVAMGEVRALGASTATVAGWGYSGLGIDVGNPHLACVDPT
CCCCCCCEEEEECCCCCCEEEEEECCEEECCCCCEEEECCCCCCEEEECCCCEEEEECCC
LTAEALAKLDLTVSPGYDPDLFPHGVNVEILTPLDEQRAVDMRVYERGVGETRSCGTGTV
HHHHHHHEEEEEECCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCHH
AAAAAALSAEGFELATGSGAVTVRVPGGAVRVGLEAGSAWLRGPSVLVATGRLTADWWQT
HHHHHHHCCCCEEEECCCCEEEEEECCCEEEEEEECCCCEECCCEEEEEECCCCHHHHHH
LG
CC
>Mature Secondary Structure 
TDIGGTDIEFTKGHGTENDFVVLPDEDVRLDLTPARVAALCDRQRGLGADGVLRVARAG
CCCCCCCEEEECCCCCCCCEEEECCCCCEEEECHHHHHHHHHHHCCCCHHHHHHHHHHH
ALLRAGVLDALPAGVSDTDWFMDYRNADGSIAEMCGNGVRVFAHYLAATGRAEGTEHVVG
HHHHHHHHHHCCCCCCCCHHEEEECCCCCHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHC
SRAGARPVTVHAAGPTHGEVTVAMGEVRALGASTATVAGWGYSGLGIDVGNPHLACVDPT
CCCCCCCEEEEECCCCCCEEEEEECCEEECCCCCEEEECCCCCCEEEECCCCEEEEECCC
LTAEALAKLDLTVSPGYDPDLFPHGVNVEILTPLDEQRAVDMRVYERGVGETRSCGTGTV
HHHHHHHEEEEEECCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCHH
AAAAAALSAEGFELATGSGAVTVRVPGGAVRVGLEAGSAWLRGPSVLVATGRLTADWWQT
HHHHHHHCCCCEEEECCCCEEEEEECCCEEEEEEECCCCEECCCEEEEEECCCCHHHHHH
LG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA