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The map label for this gene is aspS

Identifier: 54025619

GI number: 54025619

Start: 3863075

End: 3864868

Strand: Reverse

Name: aspS

Synonym: nfa36490

Alternate gene names: 54025619

Gene position: 3864868-3863075 (Counterclockwise)

Preceding gene: 54025622

Following gene: 54025617

Centisome position: 64.19

GC content: 70.07

Gene sequence:

>1794_bases
GTGCTGCGCACCCACTTGGCTGGTTCGCTGCGAAGCGAGCATGCCGGTCAGACCGTCACCCTCACCGGATGGGTGGCCCG
GCGGCGCGACCACGGTGGGGTGATCTTCATCGATCTGCGCGACGCCTCGGGGGTGTCGCAGGCGGTGTTCCGCGAGGGCG
AGGCCGCCGAACAGGCGCATCGGCTGCGCGCGGAGTACTGCGTGCGGGTCACCGGCGTGGTCGAGCAGCGGCCCGACGGC
AACGAGAACCCGGAACTGCCGACCGGGCAGATCGAGGTGAACGTCCGCGAGCTCGAGGTGCTCAACGAGAGCGCGCCGCT
GCCGTTCCAGCTCGACGAGCAGCCCGGCGAGGAGGCCCGTCTCAAGCACCGCTACCTGGACCTGCGCCGCGAGGGCCCGG
CGCATGCCATCCGGCTGCGCTCCAAGGTCAACGCCGCTGCCCGCGAGGTGCTGGCCCGGCACGAGTTCGTCGAGGTGGAG
ACCCCGACGCTGACCAAGTCCACGCCCGAGGGCGCGCGTGACTTCCTGGTGCCCGCGCGCCTGCAGCCGGGCAACTTCTA
CGCGCTGCCGCAGAGCCCGCAGCTGTTCAAGCAGCTGCTCATGGTGGGCGGCATCGAACGGTACTTCCAGATCGCCCGCT
GCTACCGCGACGAGGACTTCCGTGCCGACCGGCAGCCGGAATTCACCCAGCTCGACATCGAGATGAGCTTCGTGGAGCAG
GCCGACGTGATCCTGCTCGCCGAGGAGATCCTGGTCGCGTTGTGGAAGCTGATCGGCTACGAGATCCCGACCCCGATCCC
GCACATGACCTACGCCGAGGCGATGCGCCGCTTCGGTTCCGACAAGCCCGACCTGCGGTTCGGCGTCGAGATCACCGAGT
GCACCGAGTACTTCAAGAACACCCCGTTCCGGGTGTTCCAGGCGCCCTACGTCGGCGCCGTGGTGATGCCCGGTGGGGCG
AGTCAGCCGCGGCGCCAACTCGACGCCTGGCAGGAGTGGGCCAAGCAGCGCGGGGCGAAGGGCCTGGCGTATGTGCTGGT
CAACGAGGACGGCAGTCTCGGAGGTCCGGTCGCCAAGAACCTCAGCGACGCCGAGCGCGAGGGGCTGGCCAAGCACGTGG
GCGCGGAGCCGGGCGACTGCGTGTTCTTCGCCGCGGGCCCGGCCAAGGCGCAGCGGGCGCTGCTCGGCGCGGCGCGCGGG
GAGATCGCGCGCAAGGTCGGCCTGATCGACGAGAACGCCTGGTCGTTCGTGTGGATCGTGGACGCGCCGTTGTTCGAGCC
CGCCGACGACGCGACCGCCAGCGGTGACGTCGCGCTCGGGCACTCGGCGTGGACGGCCGTGCATCACGCGTTCACTTCGC
CCAAGCCGGAGTCGCTGGACACCTTCGACACCGACCCGGGGTCGGCGTTGGCCTACGCCTACGACATCGTCTGCAACGGC
AACGAGATCGGTGGCGGCTCGATCCGTATCCACCGCCGTGATGTGCAGGAGCGGGTGTTCAAGGTGATGGGTATCGGCGA
GCAGGAGGCGCAGGAGAAGTTCGGCTTCCTGCTGGACGCCTTCGCCTACGGCGCGCCCCCGCACGGCGGCATCGCCTTCG
GCTGGGACCGCATCGTCGCGCTGCTGGCCGGACTGGACTCGATCCGCGAGGTGATCGCTTTCCCGAAGACCGGCGGCGGC
GTCGACCCGCTCACCGACGCGCCCGCCCCGATCACCGCGCAGCAGCGCAAGGAGGCGGGGCTGGACGCCAAGCCGGAACA
GGCCGAGGTGAAGAAGGCCGAACAGGCGGGCTGA

Upstream 100 bases:

>100_bases
GGCCGATATCGGTTTCCACTCGCCGATCGTGTCTCACTACGATGGCAAGCGCACCTGGTCGACGCCCGTACCGGTGTGAG
AAATCGTCGAAAGGAATCCC

Downstream 100 bases:

>100_bases
GGCTCCCCCACGACGGAATCGGCCGCGGCCCCGGACGGGGTCGCGGCCGATTCGCGGTAGCGCGACGGAAGCGCCTCAGG
CGGGAACCAGTCTGCCGCCG

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 597; Mature: 597

Protein sequence:

>597_residues
MLRTHLAGSLRSEHAGQTVTLTGWVARRRDHGGVIFIDLRDASGVSQAVFREGEAAEQAHRLRAEYCVRVTGVVEQRPDG
NENPELPTGQIEVNVRELEVLNESAPLPFQLDEQPGEEARLKHRYLDLRREGPAHAIRLRSKVNAAAREVLARHEFVEVE
TPTLTKSTPEGARDFLVPARLQPGNFYALPQSPQLFKQLLMVGGIERYFQIARCYRDEDFRADRQPEFTQLDIEMSFVEQ
ADVILLAEEILVALWKLIGYEIPTPIPHMTYAEAMRRFGSDKPDLRFGVEITECTEYFKNTPFRVFQAPYVGAVVMPGGA
SQPRRQLDAWQEWAKQRGAKGLAYVLVNEDGSLGGPVAKNLSDAEREGLAKHVGAEPGDCVFFAAGPAKAQRALLGAARG
EIARKVGLIDENAWSFVWIVDAPLFEPADDATASGDVALGHSAWTAVHHAFTSPKPESLDTFDTDPGSALAYAYDIVCNG
NEIGGGSIRIHRRDVQERVFKVMGIGEQEAQEKFGFLLDAFAYGAPPHGGIAFGWDRIVALLAGLDSIREVIAFPKTGGG
VDPLTDAPAPITAQQRKEAGLDAKPEQAEVKKAEQAG

Sequences:

>Translated_597_residues
MLRTHLAGSLRSEHAGQTVTLTGWVARRRDHGGVIFIDLRDASGVSQAVFREGEAAEQAHRLRAEYCVRVTGVVEQRPDG
NENPELPTGQIEVNVRELEVLNESAPLPFQLDEQPGEEARLKHRYLDLRREGPAHAIRLRSKVNAAAREVLARHEFVEVE
TPTLTKSTPEGARDFLVPARLQPGNFYALPQSPQLFKQLLMVGGIERYFQIARCYRDEDFRADRQPEFTQLDIEMSFVEQ
ADVILLAEEILVALWKLIGYEIPTPIPHMTYAEAMRRFGSDKPDLRFGVEITECTEYFKNTPFRVFQAPYVGAVVMPGGA
SQPRRQLDAWQEWAKQRGAKGLAYVLVNEDGSLGGPVAKNLSDAEREGLAKHVGAEPGDCVFFAAGPAKAQRALLGAARG
EIARKVGLIDENAWSFVWIVDAPLFEPADDATASGDVALGHSAWTAVHHAFTSPKPESLDTFDTDPGSALAYAYDIVCNG
NEIGGGSIRIHRRDVQERVFKVMGIGEQEAQEKFGFLLDAFAYGAPPHGGIAFGWDRIVALLAGLDSIREVIAFPKTGGG
VDPLTDAPAPITAQQRKEAGLDAKPEQAEVKKAEQAG
>Mature_597_residues
MLRTHLAGSLRSEHAGQTVTLTGWVARRRDHGGVIFIDLRDASGVSQAVFREGEAAEQAHRLRAEYCVRVTGVVEQRPDG
NENPELPTGQIEVNVRELEVLNESAPLPFQLDEQPGEEARLKHRYLDLRREGPAHAIRLRSKVNAAAREVLARHEFVEVE
TPTLTKSTPEGARDFLVPARLQPGNFYALPQSPQLFKQLLMVGGIERYFQIARCYRDEDFRADRQPEFTQLDIEMSFVEQ
ADVILLAEEILVALWKLIGYEIPTPIPHMTYAEAMRRFGSDKPDLRFGVEITECTEYFKNTPFRVFQAPYVGAVVMPGGA
SQPRRQLDAWQEWAKQRGAKGLAYVLVNEDGSLGGPVAKNLSDAEREGLAKHVGAEPGDCVFFAAGPAKAQRALLGAARG
EIARKVGLIDENAWSFVWIVDAPLFEPADDATASGDVALGHSAWTAVHHAFTSPKPESLDTFDTDPGSALAYAYDIVCNG
NEIGGGSIRIHRRDVQERVFKVMGIGEQEAQEKFGFLLDAFAYGAPPHGGIAFGWDRIVALLAGLDSIREVIAFPKTGGG
VDPLTDAPAPITAQQRKEAGLDAKPEQAEVKKAEQAG

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=613, Percent_Identity=40.9461663947798, Blast_Score=447, Evalue=1e-125,
Organism=Homo sapiens, GI45439306, Length=249, Percent_Identity=29.718875502008, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI5031815, Length=252, Percent_Identity=28.1746031746032, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI194272210, Length=252, Percent_Identity=28.1746031746032, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1788173, Length=580, Percent_Identity=47.2413793103448, Blast_Score=538, Evalue=1e-154,
Organism=Escherichia coli, GI1789256, Length=315, Percent_Identity=26.031746031746, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1790571, Length=289, Percent_Identity=27.3356401384083, Blast_Score=93, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI32566633, Length=607, Percent_Identity=35.7495881383855, Blast_Score=337, Evalue=9e-93,
Organism=Caenorhabditis elegans, GI71984122, Length=317, Percent_Identity=26.4984227129338, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI71994340, Length=140, Percent_Identity=31.4285714285714, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17535925, Length=140, Percent_Identity=31.4285714285714, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17535927, Length=140, Percent_Identity=31.4285714285714, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6325153, Length=630, Percent_Identity=31.9047619047619, Blast_Score=271, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6321807, Length=263, Percent_Identity=27.3764258555133, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323011, Length=278, Percent_Identity=27.6978417266187, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24584738, Length=604, Percent_Identity=38.9072847682119, Blast_Score=364, Evalue=1e-100,
Organism=Drosophila melanogaster, GI17136276, Length=250, Percent_Identity=25.6, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24640849, Length=251, Percent_Identity=25.8964143426295, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24640851, Length=251, Percent_Identity=25.8964143426295, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19921528, Length=280, Percent_Identity=26.4285714285714, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_NOCFA (Q5YTJ4)

Other databases:

- EMBL:   AP006618
- RefSeq:   YP_119861.1
- ProteinModelPortal:   Q5YTJ4
- SMR:   Q5YTJ4
- GeneID:   3109879
- GenomeReviews:   AP006618_GR
- KEGG:   nfa:nfa36490
- NMPDR:   fig|247156.1.peg.3561
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- ProtClustDB:   PRK00476
- BioCyc:   NFAR247156:NFA36490-MONOMER
- BRENDA:   6.1.1.12
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 65560; Mature: 65560

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRTHLAGSLRSEHAGQTVTLTGWVARRRDHGGVIFIDLRDASGVSQAVFREGEAAEQAH
CCCCHHHHHHHHHCCCCEEEEEEHHHHHCCCCCEEEEEECCCCCHHHHHHHCCHHHHHHH
RLRAEYCVRVTGVVEQRPDGNENPELPTGQIEVNVRELEVLNESAPLPFQLDEQPGEEAR
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHCCCCCCCEEECCCCCHHHH
LKHRYLDLRREGPAHAIRLRSKVNAAAREVLARHEFVEVETPTLTKSTPEGARDFLVPAR
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHCCCCCE
LQPGNFYALPQSPQLFKQLLMVGGIERYFQIARCYRDEDFRADRQPEFTQLDIEMSFVEQ
ECCCCEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEHHHCC
ADVILLAEEILVALWKLIGYEIPTPIPHMTYAEAMRRFGSDKPDLRFGVEITECTEYFKN
CCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCHHHHHHHHCC
TPFRVFQAPYVGAVVMPGGASQPRRQLDAWQEWAKQRGAKGLAYVLVNEDGSLGGPVAKN
CCEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHC
LSDAEREGLAKHVGAEPGDCVFFAAGPAKAQRALLGAARGEIARKVGLIDENAWSFVWIV
CCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCEECCCEEEEEEE
DAPLFEPADDATASGDVALGHSAWTAVHHAFTSPKPESLDTFDTDPGSALAYAYDIVCNG
ECCCCCCCCCCCCCCCEEECHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEEECC
NEIGGGSIRIHRRDVQERVFKVMGIGEQEAQEKFGFLLDAFAYGAPPHGGIAFGWDRIVA
CEECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHH
LLAGLDSIREVIAFPKTGGGVDPLTDAPAPITAQQRKEAGLDAKPEQAEVKKAEQAG
HHHCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MLRTHLAGSLRSEHAGQTVTLTGWVARRRDHGGVIFIDLRDASGVSQAVFREGEAAEQAH
CCCCHHHHHHHHHCCCCEEEEEEHHHHHCCCCCEEEEEECCCCCHHHHHHHCCHHHHHHH
RLRAEYCVRVTGVVEQRPDGNENPELPTGQIEVNVRELEVLNESAPLPFQLDEQPGEEAR
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHCCCCCCCEEECCCCCHHHH
LKHRYLDLRREGPAHAIRLRSKVNAAAREVLARHEFVEVETPTLTKSTPEGARDFLVPAR
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHCCCCCE
LQPGNFYALPQSPQLFKQLLMVGGIERYFQIARCYRDEDFRADRQPEFTQLDIEMSFVEQ
ECCCCEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEHHHCC
ADVILLAEEILVALWKLIGYEIPTPIPHMTYAEAMRRFGSDKPDLRFGVEITECTEYFKN
CCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCHHHHHHHHCC
TPFRVFQAPYVGAVVMPGGASQPRRQLDAWQEWAKQRGAKGLAYVLVNEDGSLGGPVAKN
CCEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHC
LSDAEREGLAKHVGAEPGDCVFFAAGPAKAQRALLGAARGEIARKVGLIDENAWSFVWIV
CCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCEECCCEEEEEEE
DAPLFEPADDATASGDVALGHSAWTAVHHAFTSPKPESLDTFDTDPGSALAYAYDIVCNG
ECCCCCCCCCCCCCCCEEECHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEEECC
NEIGGGSIRIHRRDVQERVFKVMGIGEQEAQEKFGFLLDAFAYGAPPHGGIAFGWDRIVA
CEECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHH
LLAGLDSIREVIAFPKTGGGVDPLTDAPAPITAQQRKEAGLDAKPEQAEVKKAEQAG
HHHCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA