Click here to switch to the map view.

The map label for this gene is pyrB

Identifier: 54025593

GI number: 54025593

Start: 3836412

End: 3837371

Strand: Reverse

Name: pyrB

Synonym: nfa36230

Alternate gene names: 54025593

Gene position: 3837371-3836412 (Counterclockwise)

Preceding gene: 54025594

Following gene: 54025592

Centisome position: 63.73

GC content: 71.35

Gene sequence:

>960_bases
GTGCGACATCTGCTGAGCGTGACCGATCTGGATCGCACCGCCGCCACCGAGCTGCTCGACGAGGCCGAACGCTTCGAACA
GGCGCTGCTCGGACGCGAGGTGCACAAGCTGCCGACGCTGCGCGGACGCACCGTGATGACGGTCTTCTACGAGAACTCCA
CGCGCACCCGGGTGTCCTTCGAGGTGGCGGGCAAGTGGATGAGCGCCGACGTCATCAACGTCAGCGCGAGTTCGTCCTCG
GTGTCCAAGGGTGAGTCGCTGCGCGACACCGCGCTCACCCTGCACGCCGCGGGCGCGGACGCGCTGATCGTGCGCCATCC
CGCCTCCGGCGCCGCCCATCAGATCGCGCGGTGGATGGACGGGTGGGCGCGCGAGTCGGGCCGCTCCCACGGTCCGGCGA
TCGTCAACGCGGGCGACGGCATGCACGAGCACCCCACCCAGGCCCTGCTGGACGCGCTGACCCTGCGGCAGCGCCTCGGC
GACATCGAGGGCAAACGGGTGGTCATCGTCGGCGACATCCTGCACAGCCGGGTCGCCCGCTCGAACGTCTTCCTGCTGCA
CACCCTCGGCGCGGAGGTGGTGCTGGTCGCGCCGCGCACGCTGCTGCCGGTGGGCGTACAGAGCTGGCCGGCCCGCGTCT
CCCACCACCTCGACGCCGAACTGCCCGGCGCCGACGCGGTGCTGATGCTGCGGGTCCAGGCCGAACGCATGAACGGCGGG
TTCTTCCCGTCGGCGCGGGAGTACTCGGTCAACTACGGCCTCTCCGAGCGCAGGCTCGCGCTGCTGGCCGAGCACGCGGT
GGTGATGCACCCCGGGCCGATGCTGCGCGGCATGGAGATCGCCTCCGCGGTCGCGGATTCGCCGCAGGCCGCCGTCCTGC
AGCAGGTGACCAACGGTGTGCACCTGCGGATGGCGGTGCTGTTCCGGCTGCTGGTCGGGGCGCAGGAGGCGATCGCGTGA

Upstream 100 bases:

>100_bases
CAAGAACGTGCCCACCTCCCGCAGCGAGGACATCTCGGTGCTGCTCACCGAGCACGACGGTCGCGACGGCGTCTACCTCC
ACCACGAGGAGGGCTAGCCG

Downstream 100 bases:

>100_bases
ACGGCGGGGCACACGACCGGGAGGAGCGGGCATGAGTGGACTGTTGATCAAGGGCGCCGTCCTCTACGGCGAGGGTGAGC
CGGTCGACGTCTTCGTCGAC

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MRHLLSVTDLDRTAATELLDEAERFEQALLGREVHKLPTLRGRTVMTVFYENSTRTRVSFEVAGKWMSADVINVSASSSS
VSKGESLRDTALTLHAAGADALIVRHPASGAAHQIARWMDGWARESGRSHGPAIVNAGDGMHEHPTQALLDALTLRQRLG
DIEGKRVVIVGDILHSRVARSNVFLLHTLGAEVVLVAPRTLLPVGVQSWPARVSHHLDAELPGADAVLMLRVQAERMNGG
FFPSAREYSVNYGLSERRLALLAEHAVVMHPGPMLRGMEIASAVADSPQAAVLQQVTNGVHLRMAVLFRLLVGAQEAIA

Sequences:

>Translated_319_residues
MRHLLSVTDLDRTAATELLDEAERFEQALLGREVHKLPTLRGRTVMTVFYENSTRTRVSFEVAGKWMSADVINVSASSSS
VSKGESLRDTALTLHAAGADALIVRHPASGAAHQIARWMDGWARESGRSHGPAIVNAGDGMHEHPTQALLDALTLRQRLG
DIEGKRVVIVGDILHSRVARSNVFLLHTLGAEVVLVAPRTLLPVGVQSWPARVSHHLDAELPGADAVLMLRVQAERMNGG
FFPSAREYSVNYGLSERRLALLAEHAVVMHPGPMLRGMEIASAVADSPQAAVLQQVTNGVHLRMAVLFRLLVGAQEAIA
>Mature_319_residues
MRHLLSVTDLDRTAATELLDEAERFEQALLGREVHKLPTLRGRTVMTVFYENSTRTRVSFEVAGKWMSADVINVSASSSS
VSKGESLRDTALTLHAAGADALIVRHPASGAAHQIARWMDGWARESGRSHGPAIVNAGDGMHEHPTQALLDALTLRQRLG
DIEGKRVVIVGDILHSRVARSNVFLLHTLGAEVVLVAPRTLLPVGVQSWPARVSHHLDAELPGADAVLMLRVQAERMNGG
FFPSAREYSVNYGLSERRLALLAEHAVVMHPGPMLRGMEIASAVADSPQAAVLQQVTNGVHLRMAVLFRLLVGAQEAIA

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=320, Percent_Identity=34.0625, Blast_Score=157, Evalue=1e-38,
Organism=Escherichia coli, GI2367364, Length=323, Percent_Identity=28.7925696594427, Blast_Score=116, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI193204318, Length=339, Percent_Identity=33.6283185840708, Blast_Score=124, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6322331, Length=318, Percent_Identity=33.6477987421384, Blast_Score=136, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24642586, Length=331, Percent_Identity=32.3262839879154, Blast_Score=144, Evalue=8e-35,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_NOCFA (Q5YTM0)

Other databases:

- EMBL:   AP006618
- RefSeq:   YP_119835.1
- ProteinModelPortal:   Q5YTM0
- SMR:   Q5YTM0
- GeneID:   3111426
- GenomeReviews:   AP006618_GR
- KEGG:   nfa:nfa36230
- NMPDR:   fig|247156.1.peg.3536
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   PRK00856
- BioCyc:   NFAR247156:NFA36230-MONOMER
- BRENDA:   2.1.3.2
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 34556; Mature: 34556

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHLLSVTDLDRTAATELLDEAERFEQALLGREVHKLPTLRGRTVMTVFYENSTRTRVSF
CCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCEEEEE
EVAGKWMSADVINVSASSSSVSKGESLRDTALTLHAAGADALIVRHPASGAAHQIARWMD
EECCCEEECEEEEEECCCCCCCCCCCHHHHEEEEEECCCCEEEEECCCCCHHHHHHHHHH
GWARESGRSHGPAIVNAGDGMHEHPTQALLDALTLRQRLGDIEGKRVVIVGDILHSRVAR
HHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHH
SNVFLLHTLGAEVVLVAPRTLLPVGVQSWPARVSHHLDAELPGADAVLMLRVQAERMNGG
CCEEEEEECCCCEEEECCCHHCCCCCCHHHHHHHHCCCCCCCCCCEEEEEEEEHHHCCCC
FFPSAREYSVNYGLSERRLALLAEHAVVMHPGPMLRGMEIASAVADSPQAAVLQQVTNGV
CCCCCCCEEECCCCCHHHHHHHHHCEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHCCH
HLRMAVLFRLLVGAQEAIA
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRHLLSVTDLDRTAATELLDEAERFEQALLGREVHKLPTLRGRTVMTVFYENSTRTRVSF
CCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCEEEEE
EVAGKWMSADVINVSASSSSVSKGESLRDTALTLHAAGADALIVRHPASGAAHQIARWMD
EECCCEEECEEEEEECCCCCCCCCCCHHHHEEEEEECCCCEEEEECCCCCHHHHHHHHHH
GWARESGRSHGPAIVNAGDGMHEHPTQALLDALTLRQRLGDIEGKRVVIVGDILHSRVAR
HHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHH
SNVFLLHTLGAEVVLVAPRTLLPVGVQSWPARVSHHLDAELPGADAVLMLRVQAERMNGG
CCEEEEEECCCCEEEECCCHHCCCCCCHHHHHHHHCCCCCCCCCCEEEEEEEEHHHCCCC
FFPSAREYSVNYGLSERRLALLAEHAVVMHPGPMLRGMEIASAVADSPQAAVLQQVTNGV
CCCCCCCEEECCCCCHHHHHHHHHCEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHCCH
HLRMAVLFRLLVGAQEAIA
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA