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The map label for this gene is carA [H]

Identifier: 54025590

GI number: 54025590

Start: 3833455

End: 3834573

Strand: Reverse

Name: carA [H]

Synonym: nfa36200

Alternate gene names: 54025590

Gene position: 3834573-3833455 (Counterclockwise)

Preceding gene: 54025591

Following gene: 54025589

Centisome position: 63.68

GC content: 70.51

Gene sequence:

>1119_bases
ATGAGCGCAGACACTGCAGCCGCCCTGGTGCTCGAGGACGGCCGGGTGTTCCGCGGCACGGCCTACGGAGCGGTCGGGCA
GACCCTCGGTGAGGCGGTGTTCTGCACCGCGATGACCGGGTACCAGGAGACCCTGACCGACCCGAGCTACCACCGCCAGA
TCGTGGTGGCCACCGCGCCGCAGATCGGCAACACCGGCTGGAACGACGAGGACGACGAATCGCAGCGGATCTGGGTCGCC
GGATACGCGGTGCGCGATCCCGCGCGCCGGGTCTCCAACTGGCGCGCCACCCGGTCGCTGCCCGACGAGCTGGACCGCCA
GGGCATCGTCGGCATCGCCGGCATCGACACCCGTGCCCTGGTGCGCCACCTGCGCACCCGCGGCTCGATGAAGGCGGGCA
TCTTCTCCGGCGCCGCCCTCGCCGACGCCGAGGAACTGCTGGCCCGGGTGACCGGGCAGCCCTCGATGCTGGGCGCCGAC
CTGGCCGAGGAGGTCAGCACCGACGGGGTCTACACGATCGAGCCGACCGGCGATCACCGGTTCACCGTCGTCGCCGTCGA
CCTCGGCATCAAGACCAACACCCCGCGCATGTTCGCCCAGCGCGGTATCCGGGTACACGTCGTGCCCTCGACCACCTCGC
TCGACCAGGTGCTGGAGCTGAAGCCGGACGGGGTCTTCCTGTCCAACGGGCCCGGCGACCCGGCCACCCAGAACGACGCG
GTCGCGATCACCCAGGGCGTGCTCGAACGCGGCGTGCCGCTGTTCGGCATCTGCTTCGGCAACCAGATCCTGGGCCGCGC
GCTGGGCCGGGAGACCTACAAGATGAAGTTCGGCCACCGTGGCATCAACATCCCGGTCGTCGAGCACGAGACCGGCCGCA
TCTCGATCACCGCGCAGAACCACGGGTTCGCGCTGGAAGGGGAGCGGGGCGAGCAGTTCGACACCCCGTTCGGCCGCGCC
GAGATCAGCCACGTGTGCGCCAACGACGGCACCGTCGAGGGGGTGCGCCTGCTCGACGGCCGCGCCTTCTCGGTGCAGTA
CCACCCCGAGGCCGCCGCGGGTCCGCACGATGCCGCATATCTGTTCGACCGCTTCGCCGGTCTCATGGAAGGAGCCTGA

Upstream 100 bases:

>100_bases
GGAAGCTGCCGACCGGAACCGAGATCGATACCGGGTTCCGCGGCGACGACAAGACGGTGTACCCGGCGTGGGCCGCGTCG
ATGACGGGAGAAGAGGAGAG

Downstream 100 bases:

>100_bases
TGCCACGCCGCGAGGATCTGAAGCACATCCTGGTGATCGGCTCGGGCCCGATCGTCATCGGCCAGGCCTGCGAGTTCGAC
TACTCCGGCACCCAGGCGTG

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MSADTAAALVLEDGRVFRGTAYGAVGQTLGEAVFCTAMTGYQETLTDPSYHRQIVVATAPQIGNTGWNDEDDESQRIWVA
GYAVRDPARRVSNWRATRSLPDELDRQGIVGIAGIDTRALVRHLRTRGSMKAGIFSGAALADAEELLARVTGQPSMLGAD
LAEEVSTDGVYTIEPTGDHRFTVVAVDLGIKTNTPRMFAQRGIRVHVVPSTTSLDQVLELKPDGVFLSNGPGDPATQNDA
VAITQGVLERGVPLFGICFGNQILGRALGRETYKMKFGHRGINIPVVEHETGRISITAQNHGFALEGERGEQFDTPFGRA
EISHVCANDGTVEGVRLLDGRAFSVQYHPEAAAGPHDAAYLFDRFAGLMEGA

Sequences:

>Translated_372_residues
MSADTAAALVLEDGRVFRGTAYGAVGQTLGEAVFCTAMTGYQETLTDPSYHRQIVVATAPQIGNTGWNDEDDESQRIWVA
GYAVRDPARRVSNWRATRSLPDELDRQGIVGIAGIDTRALVRHLRTRGSMKAGIFSGAALADAEELLARVTGQPSMLGAD
LAEEVSTDGVYTIEPTGDHRFTVVAVDLGIKTNTPRMFAQRGIRVHVVPSTTSLDQVLELKPDGVFLSNGPGDPATQNDA
VAITQGVLERGVPLFGICFGNQILGRALGRETYKMKFGHRGINIPVVEHETGRISITAQNHGFALEGERGEQFDTPFGRA
EISHVCANDGTVEGVRLLDGRAFSVQYHPEAAAGPHDAAYLFDRFAGLMEGA
>Mature_371_residues
SADTAAALVLEDGRVFRGTAYGAVGQTLGEAVFCTAMTGYQETLTDPSYHRQIVVATAPQIGNTGWNDEDDESQRIWVAG
YAVRDPARRVSNWRATRSLPDELDRQGIVGIAGIDTRALVRHLRTRGSMKAGIFSGAALADAEELLARVTGQPSMLGADL
AEEVSTDGVYTIEPTGDHRFTVVAVDLGIKTNTPRMFAQRGIRVHVVPSTTSLDQVLELKPDGVFLSNGPGDPATQNDAV
AITQGVLERGVPLFGICFGNQILGRALGRETYKMKFGHRGINIPVVEHETGRISITAQNHGFALEGERGEQFDTPFGRAE
ISHVCANDGTVEGVRLLDGRAFSVQYHPEAAAGPHDAAYLFDRFAGLMEGA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=370, Percent_Identity=41.6216216216216, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI169790915, Length=383, Percent_Identity=36.5535248041775, Blast_Score=206, Evalue=4e-53,
Organism=Homo sapiens, GI21361331, Length=383, Percent_Identity=36.5535248041775, Blast_Score=205, Evalue=5e-53,
Organism=Escherichia coli, GI1786215, Length=379, Percent_Identity=46.4379947229551, Blast_Score=313, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI193204318, Length=377, Percent_Identity=40.3183023872679, Blast_Score=238, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6324878, Length=384, Percent_Identity=36.71875, Blast_Score=224, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6322331, Length=398, Percent_Identity=35.427135678392, Blast_Score=222, Evalue=8e-59,
Organism=Drosophila melanogaster, GI45555749, Length=384, Percent_Identity=39.0625, Blast_Score=223, Evalue=1e-58,
Organism=Drosophila melanogaster, GI24642586, Length=384, Percent_Identity=39.0625, Blast_Score=223, Evalue=2e-58,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 39949; Mature: 39818

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADTAAALVLEDGRVFRGTAYGAVGQTLGEAVFCTAMTGYQETLTDPSYHRQIVVATAP
CCCCCEEEEEEECCEEEEEEECHHHHHHHHHEEEEEEECCCHHHCCCCCCCCEEEEEECC
QIGNTGWNDEDDESQRIWVAGYAVRDPARRVSNWRATRSLPDELDRQGIVGIAGIDTRAL
CCCCCCCCCCCCCCCEEEEEEEEECCHHHHHHHHHHHCCCCHHHCCCCEEEEECCCHHHH
VRHLRTRGSMKAGIFSGAALADAEELLARVTGQPSMLGADLAEEVSTDGVYTIEPTGDHR
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCCCCE
FTVVAVDLGIKTNTPRMFAQRGIRVHVVPSTTSLDQVLELKPDGVFLSNGPGDPATQNDA
EEEEEEEECCCCCCCHHHHHCCCEEEEECCCCCHHHHHEECCCCEEEECCCCCCCCCCCC
VAITQGVLERGVPLFGICFGNQILGRALGRETYKMKFGHRGINIPVVEHETGRISITAQN
CHHHHHHHHCCCCEEEEECCHHHHHHHHCCCEEEEEECCCCCCEEEEECCCCEEEEEECC
HGFALEGERGEQFDTPFGRAEISHVCANDGTVEGVRLLDGRAFSVQYHPEAAAGPHDAAY
CCEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEECCEEEEEEECCCCCCCCCHHHH
LFDRFAGLMEGA
HHHHHHHHHCCC
>Mature Secondary Structure 
SADTAAALVLEDGRVFRGTAYGAVGQTLGEAVFCTAMTGYQETLTDPSYHRQIVVATAP
CCCCEEEEEEECCEEEEEEECHHHHHHHHHEEEEEEECCCHHHCCCCCCCCEEEEEECC
QIGNTGWNDEDDESQRIWVAGYAVRDPARRVSNWRATRSLPDELDRQGIVGIAGIDTRAL
CCCCCCCCCCCCCCCEEEEEEEEECCHHHHHHHHHHHCCCCHHHCCCCEEEEECCCHHHH
VRHLRTRGSMKAGIFSGAALADAEELLARVTGQPSMLGADLAEEVSTDGVYTIEPTGDHR
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCCCCE
FTVVAVDLGIKTNTPRMFAQRGIRVHVVPSTTSLDQVLELKPDGVFLSNGPGDPATQNDA
EEEEEEEECCCCCCCHHHHHCCCEEEEECCCCCHHHHHEECCCCEEEECCCCCCCCCCCC
VAITQGVLERGVPLFGICFGNQILGRALGRETYKMKFGHRGINIPVVEHETGRISITAQN
CHHHHHHHHCCCCEEEEECCHHHHHHHHCCCEEEEEECCCCCCEEEEECCCCEEEEEECC
HGFALEGERGEQFDTPFGRAEISHVCANDGTVEGVRLLDGRAFSVQYHPEAAAGPHDAAY
CCEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEECCEEEEEEECCCCCCCCCHHHH
LFDRFAGLMEGA
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA