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The map label for this gene is ribH [H]

Identifier: 54025569

GI number: 54025569

Start: 3811038

End: 3811517

Strand: Reverse

Name: ribH [H]

Synonym: nfa35990

Alternate gene names: 54025569

Gene position: 3811517-3811038 (Counterclockwise)

Preceding gene: 54025570

Following gene: 54025568

Centisome position: 63.3

GC content: 74.17

Gene sequence:

>480_bases
ATGAGCGGCACCGGTGTTCCCACCTTCGAGCTGGCCGACGCCAAGGACCTGCGCCTGGGCATCGTCGCGTCGCGCTGGCA
CACCACCATCTGCGACACCCTGGTCGCCAACGCCGAGCGCGTGGCGAAGCAGGCCGGCGTCGAGCACGTGACGGTGGTGC
GCTGCGCGGGCGCGATGGAACTGCCGGTGGTGGCCCAGGAACTGGCCCGCACGCACGACGCGGTGGTCGCGCTCGGCGTG
GTGATCCGCGGCGGCACCCCGCACTTCGAGTACGTGTGCGACGCGGTGACCGCCGGCCTGACCCGGGTGTCGCTGGACGC
GGCCACCCCGGTCGCCAACGGTGTGCTCACCACCAACACCGAGGAGCAGGCGCTGGACCGGGCGGGGCTGCCCGGTTCGG
CCGAGGACAAGGGTGAGCAGGCGTGCGCGGCGGCGCTGGACGCCGCGCTGACCCTGCGGGCGCTGCGCGGCCAGGGCTGA

Upstream 100 bases:

>100_bases
ACCAAGCGCGACCGCATGGGGCACGACCTGGTCGGGCTCGACGATCTGGATCTGGGAGAGACCGCACAGTGAGCGGTATT
CGCGGGAAAGGCATTTCGGA

Downstream 100 bases:

>100_bases
CCCATGCCGGTGGTTCGACTGTGGCGCCGCACCGATCCGGCCGCGTCCGCCGCCGCGGGGCAGCGGTGGGAGTTCGAGGT
GCGGCCGCGACGCGCGGTGC

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain [H]

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MSGTGVPTFELADAKDLRLGIVASRWHTTICDTLVANAERVAKQAGVEHVTVVRCAGAMELPVVAQELARTHDAVVALGV
VIRGGTPHFEYVCDAVTAGLTRVSLDAATPVANGVLTTNTEEQALDRAGLPGSAEDKGEQACAAALDAALTLRALRGQG

Sequences:

>Translated_159_residues
MSGTGVPTFELADAKDLRLGIVASRWHTTICDTLVANAERVAKQAGVEHVTVVRCAGAMELPVVAQELARTHDAVVALGV
VIRGGTPHFEYVCDAVTAGLTRVSLDAATPVANGVLTTNTEEQALDRAGLPGSAEDKGEQACAAALDAALTLRALRGQG
>Mature_158_residues
SGTGVPTFELADAKDLRLGIVASRWHTTICDTLVANAERVAKQAGVEHVTVVRCAGAMELPVVAQELARTHDAVVALGVV
IRGGTPHFEYVCDAVTAGLTRVSLDAATPVANGVLTTNTEEQALDRAGLPGSAEDKGEQACAAALDAALTLRALRGQG

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786617, Length=146, Percent_Identity=45.2054794520548, Blast_Score=108, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324429, Length=164, Percent_Identity=36.5853658536585, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002180 [H]

Pfam domain/function: PF00885 DMRL_synthase [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 16372; Mature: 16241

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGTGVPTFELADAKDLRLGIVASRWHTTICDTLVANAERVAKQAGVEHVTVVRCAGAME
CCCCCCCCEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
LPVVAQELARTHDAVVALGVVIRGGTPHFEYVCDAVTAGLTRVSLDAATPVANGVLTTNT
CHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHEECCCCCCCCCCEEECCC
EEQALDRAGLPGSAEDKGEQACAAALDAALTLRALRGQG
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SGTGVPTFELADAKDLRLGIVASRWHTTICDTLVANAERVAKQAGVEHVTVVRCAGAME
CCCCCCCEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
LPVVAQELARTHDAVVALGVVIRGGTPHFEYVCDAVTAGLTRVSLDAATPVANGVLTTNT
CHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHEECCCCCCCCCCEEECCC
EEQALDRAGLPGSAEDKGEQACAAALDAALTLRALRGQG
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA