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The map label for this gene is menE [H]

Identifier: 54025512

GI number: 54025512

Start: 3753069

End: 3754616

Strand: Reverse

Name: menE [H]

Synonym: nfa35420

Alternate gene names: 54025512

Gene position: 3754616-3753069 (Counterclockwise)

Preceding gene: 54025513

Following gene: 54025511

Centisome position: 62.36

GC content: 70.87

Gene sequence:

>1548_bases
ATGACGGATTTCCCGGACACCCACGCCGCCGCCGACCCGGACCGCCCGGCCTACATCATGGCCGAGACGGACACGGTGGT
GACCTACCGGCACCTCGTCGACGCCTCCCGCGCGGTGGCGGCACTGCTGTGGTCGCGCGGGCTGCGCCACGGCGACTGTG
TCGTGCTCCTGATGGAGAACCATGTCGACTTCCCCAAGATCGCCTGGGCGGCCCAGCGCATCGGCCTGCGCTACGTGACC
ATCTCGACCCGGCTGTTGCCCGAGGAGGTCGCCTACATCCTCGCCGACAGCGGCGCGCGGGCGCTGTTCACCAGCGCCCG
CCACGCCGACGTCGCCGCGGCCGCGTCGGCACGGGTACCCGCCGTGCGCGAGCGGTTCGACGTCGACGCCGACCGGCCCG
GATTCGAAAACGTCAGCGCGGCAGTGGCATCGGTGCCGGACGGGGTGCGGCCCGATGAACGCGAGGGCGTGGATCTGCTG
TACTCCTCGGGCACCACCGGCAGGCCCAAAGGCGTGGTGGCGGAGCTGCCGCTGGCGCCGCTGGGCACGCCGCCCGGTGT
CGCGGGACTGCTGCACAGCCGGTGGGGGATCGGACGCGACAGCGTCTACCTGTCCCCGGCGCCGCTGTATCACGCCGCGC
CCCTCCGGTTCACGATGACGGTGCACCGCTTCGGCGGCACCGTGATCGTCATGGAGCGGTTCGACGCCGAGGCGGCGCTG
GCGGCGATCGAACGGTACCGGGTGACGCACACGCAGATGGTGCCGACGATGTTCATCCGGATGCTCAAACTGCCCGACGA
ACAGCGGCTGCGGTACGACCTGTCGACCCTGCGCACCGTCATCCACGCGGCCGCGCCCTGTCCGCCGGACACCAAGCGCG
CCATGATCGATTGGCTGGGCCCCATCGTGCACGAGTTCTATTCGTGCACGGAGAACGCCCTGTTCACGGCGTTGGACTCG
CACGAATGGCTGGCCCACCCCGGATCGGTCGGGCGCGCGATCCTCGGGACCCCGCACATCCTCGACGACGGAGGCCGGGA
GTTGCCGCCCGGCGAACCGGGCACGATCTGGTCCGAAGGCGGGCTGATGTTCGAGTACCTGAACGATCCGGCCAAGACCG
CGGCCTCGCGCAACGAGCGGGGCTGGACGACCGTCGGCGACATCGGTTACCTCGACGAGGACGGCTACCTGTATCTGGCG
GACCGCCGTGCCGATCTCATCCTGTCCGGCGGGGTCAACGTCTACCCGCGCGAGGCCGAGGACGCCCTCGTCGTGCATCC
CAAGGTCGCCGACGCGGCGGTGTTCGGCATCCCGCACGACGAACTCGGTGAAGTCGCGCACGCCGTGGTCGTGCCCGCGC
CCGGCGTCGCGCCCGATGCGGCGCTGGCCGGCGAATTGCTCGCGTACCTGCGCGAGCGACTGGCCGGTTACAAGTGCCCG
CGCGCCATCGACTTCGAGCCGGAATTGCCCCGCGCGGCGACCGGCAAGCTCTACAAGCGGGTGCTGCGTGAGCGGTACGC
GCAGCGGGCTGCGGTCGCACCGTCCTGA

Upstream 100 bases:

>100_bases
GTCGGCGCCTTCCTCGCCGTCGCCGGTCTGGTGGGCCTGCTGTGCCTGCTGGCCATCGTCCGCCGCTACTCGCGTGCCCG
CTGAACTCGAGGACATCGCC

Downstream 100 bases:

>100_bases
GGGCCACCCGGCCCCCCACCCCCTCCCGATCCACGTCCGGCCGTGCTCTCGGCCGTCGTACCGTCGCGCCCGCGCCCGGG
CGCACGTCGCCACTCGAGAA

Product: acyl-CoA synthetase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MTDFPDTHAAADPDRPAYIMAETDTVVTYRHLVDASRAVAALLWSRGLRHGDCVVLLMENHVDFPKIAWAAQRIGLRYVT
ISTRLLPEEVAYILADSGARALFTSARHADVAAAASARVPAVRERFDVDADRPGFENVSAAVASVPDGVRPDEREGVDLL
YSSGTTGRPKGVVAELPLAPLGTPPGVAGLLHSRWGIGRDSVYLSPAPLYHAAPLRFTMTVHRFGGTVIVMERFDAEAAL
AAIERYRVTHTQMVPTMFIRMLKLPDEQRLRYDLSTLRTVIHAAAPCPPDTKRAMIDWLGPIVHEFYSCTENALFTALDS
HEWLAHPGSVGRAILGTPHILDDGGRELPPGEPGTIWSEGGLMFEYLNDPAKTAASRNERGWTTVGDIGYLDEDGYLYLA
DRRADLILSGGVNVYPREAEDALVVHPKVADAAVFGIPHDELGEVAHAVVVPAPGVAPDAALAGELLAYLRERLAGYKCP
RAIDFEPELPRAATGKLYKRVLRERYAQRAAVAPS

Sequences:

>Translated_515_residues
MTDFPDTHAAADPDRPAYIMAETDTVVTYRHLVDASRAVAALLWSRGLRHGDCVVLLMENHVDFPKIAWAAQRIGLRYVT
ISTRLLPEEVAYILADSGARALFTSARHADVAAAASARVPAVRERFDVDADRPGFENVSAAVASVPDGVRPDEREGVDLL
YSSGTTGRPKGVVAELPLAPLGTPPGVAGLLHSRWGIGRDSVYLSPAPLYHAAPLRFTMTVHRFGGTVIVMERFDAEAAL
AAIERYRVTHTQMVPTMFIRMLKLPDEQRLRYDLSTLRTVIHAAAPCPPDTKRAMIDWLGPIVHEFYSCTENALFTALDS
HEWLAHPGSVGRAILGTPHILDDGGRELPPGEPGTIWSEGGLMFEYLNDPAKTAASRNERGWTTVGDIGYLDEDGYLYLA
DRRADLILSGGVNVYPREAEDALVVHPKVADAAVFGIPHDELGEVAHAVVVPAPGVAPDAALAGELLAYLRERLAGYKCP
RAIDFEPELPRAATGKLYKRVLRERYAQRAAVAPS
>Mature_514_residues
TDFPDTHAAADPDRPAYIMAETDTVVTYRHLVDASRAVAALLWSRGLRHGDCVVLLMENHVDFPKIAWAAQRIGLRYVTI
STRLLPEEVAYILADSGARALFTSARHADVAAAASARVPAVRERFDVDADRPGFENVSAAVASVPDGVRPDEREGVDLLY
SSGTTGRPKGVVAELPLAPLGTPPGVAGLLHSRWGIGRDSVYLSPAPLYHAAPLRFTMTVHRFGGTVIVMERFDAEAALA
AIERYRVTHTQMVPTMFIRMLKLPDEQRLRYDLSTLRTVIHAAAPCPPDTKRAMIDWLGPIVHEFYSCTENALFTALDSH
EWLAHPGSVGRAILGTPHILDDGGRELPPGEPGTIWSEGGLMFEYLNDPAKTAASRNERGWTTVGDIGYLDEDGYLYLAD
RRADLILSGGVNVYPREAEDALVVHPKVADAAVFGIPHDELGEVAHAVVVPAPGVAPDAALAGELLAYLRERLAGYKCPR
AIDFEPELPRAATGKLYKRVLRERYAQRAAVAPS

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=544, Percent_Identity=28.125, Blast_Score=168, Evalue=1e-41,
Organism=Homo sapiens, GI38505220, Length=500, Percent_Identity=28.2, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI42544132, Length=532, Percent_Identity=26.5037593984962, Blast_Score=151, Evalue=2e-36,
Organism=Homo sapiens, GI122937307, Length=516, Percent_Identity=27.3255813953488, Blast_Score=142, Evalue=9e-34,
Organism=Homo sapiens, GI115511026, Length=510, Percent_Identity=26.4705882352941, Blast_Score=139, Evalue=6e-33,
Organism=Homo sapiens, GI157311624, Length=499, Percent_Identity=27.0541082164329, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI157311622, Length=499, Percent_Identity=27.0541082164329, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI58082049, Length=499, Percent_Identity=27.2545090180361, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI62865631, Length=545, Percent_Identity=25.3211009174312, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI13325055, Length=545, Percent_Identity=25.3211009174312, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI40807357, Length=539, Percent_Identity=26.3450834879406, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI187761345, Length=520, Percent_Identity=23.8461538461538, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI187761343, Length=520, Percent_Identity=23.8461538461538, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI28416953, Length=554, Percent_Identity=23.826714801444, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI253970489, Length=377, Percent_Identity=25.7294429708223, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI8923896, Length=377, Percent_Identity=25.7294429708223, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI13375727, Length=547, Percent_Identity=23.5831809872029, Blast_Score=83, Evalue=6e-16,
Organism=Escherichia coli, GI1788107, Length=543, Percent_Identity=29.097605893186, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI221142682, Length=507, Percent_Identity=29.1913214990138, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI145693145, Length=529, Percent_Identity=28.1663516068053, Blast_Score=158, Evalue=1e-39,
Organism=Escherichia coli, GI1790505, Length=559, Percent_Identity=25.4025044722719, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1786529, Length=537, Percent_Identity=25.512104283054, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1786810, Length=531, Percent_Identity=23.3521657250471, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17531443, Length=514, Percent_Identity=27.431906614786, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI32563687, Length=503, Percent_Identity=27.037773359841, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17558820, Length=494, Percent_Identity=28.9473684210526, Blast_Score=176, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17560308, Length=537, Percent_Identity=28.4916201117318, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17559526, Length=534, Percent_Identity=26.9662921348315, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17557194, Length=536, Percent_Identity=28.9179104477612, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI71994703, Length=538, Percent_Identity=25.4646840148699, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI71994694, Length=538, Percent_Identity=25.4646840148699, Blast_Score=139, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI71994690, Length=538, Percent_Identity=25.4646840148699, Blast_Score=138, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17538037, Length=533, Percent_Identity=27.7673545966229, Blast_Score=135, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI71996755, Length=499, Percent_Identity=23.4468937875752, Blast_Score=114, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI17560140, Length=500, Percent_Identity=25, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17551278, Length=556, Percent_Identity=26.0791366906475, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI71983001, Length=563, Percent_Identity=23.6234458259325, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71982997, Length=563, Percent_Identity=23.6234458259325, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71985884, Length=158, Percent_Identity=33.5443037974684, Blast_Score=72, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=549, Percent_Identity=26.4116575591985, Blast_Score=144, Evalue=4e-35,
Organism=Drosophila melanogaster, GI21355181, Length=489, Percent_Identity=27.8118609406953, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI18859661, Length=528, Percent_Identity=27.2727272727273, Blast_Score=142, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24581924, Length=527, Percent_Identity=25.8064516129032, Blast_Score=133, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24648255, Length=503, Percent_Identity=26.0437375745527, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI24648257, Length=519, Percent_Identity=25.4335260115607, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI24648253, Length=503, Percent_Identity=25.6461232604374, Blast_Score=128, Evalue=9e-30,
Organism=Drosophila melanogaster, GI24648260, Length=505, Percent_Identity=24.7524752475248, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI21356441, Length=502, Percent_Identity=24.9003984063745, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI62472339, Length=537, Percent_Identity=24.7672253258845, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24667955, Length=537, Percent_Identity=24.7672253258845, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI21358303, Length=471, Percent_Identity=24.416135881104, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI281365686, Length=332, Percent_Identity=27.710843373494, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI21356947, Length=494, Percent_Identity=25.1012145748988, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24653035, Length=514, Percent_Identity=23.1517509727626, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24667959, Length=376, Percent_Identity=26.5957446808511, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI20130357, Length=383, Percent_Identity=28.4595300261097, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI161076582, Length=528, Percent_Identity=22.3484848484848, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19922652, Length=509, Percent_Identity=23.9685658153242, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24656500, Length=515, Percent_Identity=22.5242718446602, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI28573631, Length=557, Percent_Identity=26.032315978456, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI20130353, Length=559, Percent_Identity=25.9391771019678, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24583465, Length=571, Percent_Identity=25.569176882662, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24583463, Length=571, Percent_Identity=25.569176882662, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI281364798, Length=571, Percent_Identity=25.569176882662, Blast_Score=96, Evalue=8e-20,
Organism=Drosophila melanogaster, GI45550496, Length=557, Percent_Identity=24.9551166965889, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI45552801, Length=557, Percent_Identity=24.9551166965889, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21355225, Length=393, Percent_Identity=24.9363867684478, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 56051; Mature: 55920

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDFPDTHAAADPDRPAYIMAETDTVVTYRHLVDASRAVAALLWSRGLRHGDCVVLLMEN
CCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
HVDFPKIAWAAQRIGLRYVTISTRLLPEEVAYILADSGARALFTSARHADVAAAASARVP
CCCCHHHHHHHHHCCEEEEEEECHHCHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHCCCC
AVRERFDVDADRPGFENVSAAVASVPDGVRPDEREGVDLLYSSGTTGRPKGVVAELPLAP
HHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCEEEECCCCC
LGTPPGVAGLLHSRWGIGRDSVYLSPAPLYHAAPLRFTMTVHRFGGTVIVMERFDAEAAL
CCCCCCHHHHHHHHCCCCCCCEEECCCCCCCCCCEEEEEEEEECCCEEEEEECCCHHHHH
AAIERYRVTHTQMVPTMFIRMLKLPDEQRLRYDLSTLRTVIHAAAPCPPDTKRAMIDWLG
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
PIVHEFYSCTENALFTALDSHEWLAHPGSVGRAILGTPHILDDGGRELPPGEPGTIWSEG
HHHHHHHHHCCHHHEEEECCCCHHCCCCCCCHHHHCCCCEECCCCCCCCCCCCCCEECCC
GLMFEYLNDPAKTAASRNERGWTTVGDIGYLDEDGYLYLADRRADLILSGGVNVYPREAE
CEEEEECCCHHHHHHCCCCCCCEEECCCCEECCCCEEEEECCCCEEEEECCCEECCCCCC
DALVVHPKVADAAVFGIPHDELGEVAHAVVVPAPGVAPDAALAGELLAYLRERLAGYKCP
CEEEECCCCCCEEEECCCHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCC
RAIDFEPELPRAATGKLYKRVLRERYAQRAAVAPS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TDFPDTHAAADPDRPAYIMAETDTVVTYRHLVDASRAVAALLWSRGLRHGDCVVLLMEN
CCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
HVDFPKIAWAAQRIGLRYVTISTRLLPEEVAYILADSGARALFTSARHADVAAAASARVP
CCCCHHHHHHHHHCCEEEEEEECHHCHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHCCCC
AVRERFDVDADRPGFENVSAAVASVPDGVRPDEREGVDLLYSSGTTGRPKGVVAELPLAP
HHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCEEEECCCCC
LGTPPGVAGLLHSRWGIGRDSVYLSPAPLYHAAPLRFTMTVHRFGGTVIVMERFDAEAAL
CCCCCCHHHHHHHHCCCCCCCEEECCCCCCCCCCEEEEEEEEECCCEEEEEECCCHHHHH
AAIERYRVTHTQMVPTMFIRMLKLPDEQRLRYDLSTLRTVIHAAAPCPPDTKRAMIDWLG
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
PIVHEFYSCTENALFTALDSHEWLAHPGSVGRAILGTPHILDDGGRELPPGEPGTIWSEG
HHHHHHHHHCCHHHEEEECCCCHHCCCCCCCHHHHCCCCEECCCCCCCCCCCCCCEECCC
GLMFEYLNDPAKTAASRNERGWTTVGDIGYLDEDGYLYLADRRADLILSGGVNVYPREAE
CEEEEECCCHHHHHHCCCCCCCEEECCCCEECCCCEEEEECCCCEEEEECCCEECCCCCC
DALVVHPKVADAAVFGIPHDELGEVAHAVVVPAPGVAPDAALAGELLAYLRERLAGYKCP
CEEEECCCCCCEEEECCCHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCC
RAIDFEPELPRAATGKLYKRVLRERYAQRAAVAPS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA