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The map label for this gene is folD

Identifier: 54025400

GI number: 54025400

Start: 3634547

End: 3635398

Strand: Reverse

Name: folD

Synonym: nfa34300

Alternate gene names: 54025400

Gene position: 3635398-3634547 (Counterclockwise)

Preceding gene: 54025402

Following gene: 54025397

Centisome position: 60.38

GC content: 73.0

Gene sequence:

>852_bases
GTGGATACCGTTTCGCTGACCGGCAAGGAACTCGCCGCCGCCCTCAACGCCGACACCAAGCAGCGCGCCGAAGCGCTCAC
CGCGGGGGGCGTCGCCGCGCGGCTGGCGCTGATCGTGGCCAACGACGATCCGGCCAGCGCCTGGTATGTCAACTCGCTGC
GCAAGGCCGCCGAACGGCTCGGAATCGCCTGTGAGCGTATCGATCTCGGGGCCGACGCGACCGCCGACGGCATCCGCGCC
GAACTGCGCGAACGCAGCGCCGACCCCGCCTTCGACGCCATCATGTTGCAGACCCCGCTGCCCGCGAGCGTCGCGCTCGA
CGACGTGAGCTCGGCGATCGCGGCCGACAAGGACGTCGACGGTGTGAGCCCGCTCTCGCTCGGCCTGCTCGCCGCCGGGC
TGGCGGGCTTCGTGCCCGCCACCGCGCAGGCGGTGGTGGAGTTGCTGAAGCATCACGAGATCGCGCTGTCGGGCCGCCAT
GTCGCCGTCGTCGGCCGCTCGAACGTGGTCGGCAAGCCGCTGGCACAGCTGCTGCTGGCCGAGAACGCGACGGTCACCGT
CTGCCATTCCCGCACCACCGACCTGGCCGCGATCACCTCCACGGCCGATGTGGTGGTCGCCGCCGTCGGCCGCGCCGGAC
TGGTGACCGGCGATCACGTGCGTGCGGGCGCGGTGGTCGTCGATGTCGGCACCAACGAGGCCGAGGACGGCGGCATCGTC
GGTGACGTGGACGCGGAGTCGGTGCGCGGCAAGGCCGCCGGGCTGAGCCCGGTGCCCGGCGGGGTCGGGCCGGTCACCAC
CGCGCTGCTCATGCGGCACGTCGTCGAGGCCGCCGAGCGGCACCGCGGCTGA

Upstream 100 bases:

>100_bases
GACCGGCCGCCCCGATTGCGGCGCAATTGCGGCTGCTCGCCGCGGTCGTGTGCACCCGCAGCACCGGCGCGCCGGGACGC
GAGGATTAGGCTGTCCCGAC

Downstream 100 bases:

>100_bases
CGGCTCAACCGTCGGCGGCGATCGCGGTGATGAGGGTGCGGTCCGCGCTGGTGGCCACGCTCACCACCTGCGGGTTGTAG
CTGACCGGGGCCTGGCCCGG

Product: putative methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MDTVSLTGKELAAALNADTKQRAEALTAGGVAARLALIVANDDPASAWYVNSLRKAAERLGIACERIDLGADATADGIRA
ELRERSADPAFDAIMLQTPLPASVALDDVSSAIAADKDVDGVSPLSLGLLAAGLAGFVPATAQAVVELLKHHEIALSGRH
VAVVGRSNVVGKPLAQLLLAENATVTVCHSRTTDLAAITSTADVVVAAVGRAGLVTGDHVRAGAVVVDVGTNEAEDGGIV
GDVDAESVRGKAAGLSPVPGGVGPVTTALLMRHVVEAAERHRG

Sequences:

>Translated_283_residues
MDTVSLTGKELAAALNADTKQRAEALTAGGVAARLALIVANDDPASAWYVNSLRKAAERLGIACERIDLGADATADGIRA
ELRERSADPAFDAIMLQTPLPASVALDDVSSAIAADKDVDGVSPLSLGLLAAGLAGFVPATAQAVVELLKHHEIALSGRH
VAVVGRSNVVGKPLAQLLLAENATVTVCHSRTTDLAAITSTADVVVAAVGRAGLVTGDHVRAGAVVVDVGTNEAEDGGIV
GDVDAESVRGKAAGLSPVPGGVGPVTTALLMRHVVEAAERHRG
>Mature_283_residues
MDTVSLTGKELAAALNADTKQRAEALTAGGVAARLALIVANDDPASAWYVNSLRKAAERLGIACERIDLGADATADGIRA
ELRERSADPAFDAIMLQTPLPASVALDDVSSAIAADKDVDGVSPLSLGLLAAGLAGFVPATAQAVVELLKHHEIALSGRH
VAVVGRSNVVGKPLAQLLLAENATVTVCHSRTTDLAAITSTADVVVAAVGRAGLVTGDHVRAGAVVVDVGTNEAEDGGIV
GDVDAESVRGKAAGLSPVPGGVGPVTTALLMRHVVEAAERHRG

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=288, Percent_Identity=36.8055555555556, Blast_Score=185, Evalue=4e-47,
Organism=Homo sapiens, GI222418558, Length=295, Percent_Identity=35.2542372881356, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI94721354, Length=295, Percent_Identity=34.9152542372881, Blast_Score=164, Evalue=1e-40,
Organism=Escherichia coli, GI1786741, Length=279, Percent_Identity=37.6344086021505, Blast_Score=180, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17568735, Length=260, Percent_Identity=36.1538461538462, Blast_Score=149, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6321643, Length=294, Percent_Identity=36.0544217687075, Blast_Score=174, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6319558, Length=286, Percent_Identity=36.013986013986, Blast_Score=172, Evalue=6e-44,
Organism=Drosophila melanogaster, GI17136818, Length=292, Percent_Identity=36.3013698630137, Blast_Score=173, Evalue=9e-44,
Organism=Drosophila melanogaster, GI17136816, Length=292, Percent_Identity=36.3013698630137, Blast_Score=173, Evalue=1e-43,
Organism=Drosophila melanogaster, GI62472483, Length=282, Percent_Identity=36.1702127659575, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI45551871, Length=282, Percent_Identity=36.1702127659575, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24645718, Length=282, Percent_Identity=36.1702127659575, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI17137370, Length=282, Percent_Identity=36.1702127659575, Blast_Score=162, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_NOCFA (Q5YU63)

Other databases:

- EMBL:   AP006618
- RefSeq:   YP_119642.1
- HSSP:   P11586
- ProteinModelPortal:   Q5YU63
- SMR:   Q5YU63
- GeneID:   3109677
- GenomeReviews:   AP006618_GR
- KEGG:   nfa:nfa34300
- NMPDR:   fig|247156.1.peg.3351
- HOGENOM:   HBG328751
- OMA:   DEEIFPP
- BioCyc:   NFAR247156:NFA34300-MONOMER
- BRENDA:   1.5.1.5
- BRENDA:   3.5.4.9
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 28553; Mature: 28553

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTVSLTGKELAAALNADTKQRAEALTAGGVAARLALIVANDDPASAWYVNSLRKAAERL
CCCCCCCHHHHHHHHCCCHHHHHHHHHHCCHHHEEEEEEECCCCCHHHHHHHHHHHHHHH
GIACERIDLGADATADGIRAELRERSADPAFDAIMLQTPLPASVALDDVSSAIAADKDVD
CCCCEEEECCCCCCHHHHHHHHHHCCCCCCHHEEEECCCCCHHHHHHHHHHHHHCCCCCC
GVSPLSLGLLAAGLAGFVPATAQAVVELLKHHEIALSGRHVAVVGRSNVVGKPLAQLLLA
CCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHEEECCCEEEEEECCCCCHHHHHHHHHH
ENATVTVCHSRTTDLAAITSTADVVVAAVGRAGLVTGDHVRAGAVVVDVGTNEAEDGGIV
CCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEECCCEECCEEEEECCCCCCCCCCEE
GDVDAESVRGKAAGLSPVPGGVGPVTTALLMRHVVEAAERHRG
ECCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDTVSLTGKELAAALNADTKQRAEALTAGGVAARLALIVANDDPASAWYVNSLRKAAERL
CCCCCCCHHHHHHHHCCCHHHHHHHHHHCCHHHEEEEEEECCCCCHHHHHHHHHHHHHHH
GIACERIDLGADATADGIRAELRERSADPAFDAIMLQTPLPASVALDDVSSAIAADKDVD
CCCCEEEECCCCCCHHHHHHHHHHCCCCCCHHEEEECCCCCHHHHHHHHHHHHHCCCCCC
GVSPLSLGLLAAGLAGFVPATAQAVVELLKHHEIALSGRHVAVVGRSNVVGKPLAQLLLA
CCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHEEECCCEEEEEECCCCCHHHHHHHHHH
ENATVTVCHSRTTDLAAITSTADVVVAAVGRAGLVTGDHVRAGAVVVDVGTNEAEDGGIV
CCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEECCCEECCEEEEECCCCCCCCCCEE
GDVDAESVRGKAAGLSPVPGGVGPVTTALLMRHVVEAAERHRG
ECCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA